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The map label for this gene is ycnB

Identifier: 16077452

GI number: 16077452

Start: 436036

End: 437454

Strand: Reverse

Name: ycnB

Synonym: BSU03840

Alternate gene names: 16077452

Gene position: 437454-436036 (Counterclockwise)

Preceding gene: 255767120

Following gene: 16077447

Centisome position: 10.38

GC content: 49.68

Gene sequence:

>1419_bases
TTGAACACAAGTATTGAACAAAAACCTTTTAACCGTTCTGTCATTGTCGGCATTTTGTTAGCCGGAGCGTTTGTGGCGAT
TTTGAACCAGACATTGTTGATTACTGCACTTCCCCATATCATGAGGGATTTCAATGTCGATGCCAACCAAGCACAATGGC
TGACAACATCATTTATGTTAACCAACGGGATTTTAATTCCGATTACCGCGTTTTTAATTGAGAAATTCACAAGCCGGGCG
CTGCTCATCACAGCAATGAGCATTTTCACTGCCGGAACAGTCGTCGGAGCGTTCGCGCCGAACTTCCCGGTTCTGCTGAC
AGCGCGTATCATTCAAGCGGCTGGAGCCGGCATTATGATGCCGCTCATGCAGACTGTATTCCTGACAATCTTTCCGATTG
AAAAGCGCGGCCAGGCTATGGGTATGGTCGGATTGGTCATCTCATTCGCGCCTGCGATCGGACCGACTCTTTCCGGATGG
GCTGTCGAAGCTTTCTCTTGGAGATCATTGTTTTATATTATTCTTCCGTTTGCTGTGATTGATTTGATTCTTGCCAGCAT
CCTGATGAAAAACGTGACGACTTTAAGAAAAACACAGATTGATATTTTATCAGTCATCCTTTCAACATTTGGGTTCGGCG
GCCTTCTGTACGGCTTCTCAAGCGTCGGCTCTTATGGCTGGTCCAGTTCAACCGTCTTGATTTCACTGCTGGTGGGTGTC
ATCGCGCTGCTCTTGTTTATCACAAGACAAATGAAGCTTAAAAAACCGATGCTGGAATTCCGCGTCTTCACGTTTGGCGT
TTTCAGCTTAACAACACTGCTGGGAACCCTCGTCTTTGCATTATTGATCGGTACGGAAACCATCCTGCCGCTTTATACAC
AAAATGTCAGAGACGTCACAGCTTTTGATACAGGGCTTATGCTCCTGCCGGGAGCCGTTGTGATGGGCTTTATGTCGCCG
ATTATCGGCAGAATTTTTGACCGTGTCGGCGGAAGAGGGCTGGCGATTGCCGGTTTCTGCATCATCTTCCTGACATCTCT
GCCGTTTATGCAGCTGACTGACCATACGTCACTCGCTTGGATTGTCGTTTTATACACCGTTCGTCTCTTAGGCACCGCCA
TGATCATGATGCCGGTGACAACAGCCGGAATCAATGCGCTGCCGCGCCACTTGATTCCGCACGGAACTGCGATGAACAAC
ACGATTCGCCAAGTCGGCGGCTCGATCGGAACCGCACTATTGGTTTCCGTGATGAGCAACCAGGCGGCACATGCAGGCAC
GACAAATGTGAAGCACGCCGCCCTGCACGGCATGAACGCCGCTTTTATAGTGGCTGCCGTCATTGCACTTGTCGGCTTCC
TTCTCTCATTCACATTAAAGAAACCTCAGCGCCCTGCCGAACAGCAGCCGGCACGCTGA

Upstream 100 bases:

>100_bases
AAATCACTGATTGGCTATCTGGAACAAACCGGTGTTTTAACACAGCCCGTTTCCATGTTAAAAACACTGTTAATTACTTA
GAGAAAAGAAGGGATATGTA

Downstream 100 bases:

>100_bases
TTAACAAAAAAAGAGCCCCGCTATTTAGCGGAGGCTCTTTTTGGTTTTACTTTTCTAAACCGTCTTTCACTTCTTTAATC
ATTTGCGTCATAGACTCAAG

Product: efflux transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MNTSIEQKPFNRSVIVGILLAGAFVAILNQTLLITALPHIMRDFNVDANQAQWLTTSFMLTNGILIPITAFLIEKFTSRA
LLITAMSIFTAGTVVGAFAPNFPVLLTARIIQAAGAGIMMPLMQTVFLTIFPIEKRGQAMGMVGLVISFAPAIGPTLSGW
AVEAFSWRSLFYIILPFAVIDLILASILMKNVTTLRKTQIDILSVILSTFGFGGLLYGFSSVGSYGWSSSTVLISLLVGV
IALLLFITRQMKLKKPMLEFRVFTFGVFSLTTLLGTLVFALLIGTETILPLYTQNVRDVTAFDTGLMLLPGAVVMGFMSP
IIGRIFDRVGGRGLAIAGFCIIFLTSLPFMQLTDHTSLAWIVVLYTVRLLGTAMIMMPVTTAGINALPRHLIPHGTAMNN
TIRQVGGSIGTALLVSVMSNQAAHAGTTNVKHAALHGMNAAFIVAAVIALVGFLLSFTLKKPQRPAEQQPAR

Sequences:

>Translated_472_residues
MNTSIEQKPFNRSVIVGILLAGAFVAILNQTLLITALPHIMRDFNVDANQAQWLTTSFMLTNGILIPITAFLIEKFTSRA
LLITAMSIFTAGTVVGAFAPNFPVLLTARIIQAAGAGIMMPLMQTVFLTIFPIEKRGQAMGMVGLVISFAPAIGPTLSGW
AVEAFSWRSLFYIILPFAVIDLILASILMKNVTTLRKTQIDILSVILSTFGFGGLLYGFSSVGSYGWSSSTVLISLLVGV
IALLLFITRQMKLKKPMLEFRVFTFGVFSLTTLLGTLVFALLIGTETILPLYTQNVRDVTAFDTGLMLLPGAVVMGFMSP
IIGRIFDRVGGRGLAIAGFCIIFLTSLPFMQLTDHTSLAWIVVLYTVRLLGTAMIMMPVTTAGINALPRHLIPHGTAMNN
TIRQVGGSIGTALLVSVMSNQAAHAGTTNVKHAALHGMNAAFIVAAVIALVGFLLSFTLKKPQRPAEQQPAR
>Mature_472_residues
MNTSIEQKPFNRSVIVGILLAGAFVAILNQTLLITALPHIMRDFNVDANQAQWLTTSFMLTNGILIPITAFLIEKFTSRA
LLITAMSIFTAGTVVGAFAPNFPVLLTARIIQAAGAGIMMPLMQTVFLTIFPIEKRGQAMGMVGLVISFAPAIGPTLSGW
AVEAFSWRSLFYIILPFAVIDLILASILMKNVTTLRKTQIDILSVILSTFGFGGLLYGFSSVGSYGWSSSTVLISLLVGV
IALLLFITRQMKLKKPMLEFRVFTFGVFSLTTLLGTLVFALLIGTETILPLYTQNVRDVTAFDTGLMLLPGAVVMGFMSP
IIGRIFDRVGGRGLAIAGFCIIFLTSLPFMQLTDHTSLAWIVVLYTVRLLGTAMIMMPVTTAGINALPRHLIPHGTAMNN
TIRQVGGSIGTALLVSVMSNQAAHAGTTNVKHAALHGMNAAFIVAAVIALVGFLLSFTLKKPQRPAEQQPAR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family

Homologues:

Organism=Escherichia coli, GI1788710, Length=406, Percent_Identity=26.1083743842365, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1789042, Length=435, Percent_Identity=25.9770114942529, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI87081983, Length=415, Percent_Identity=26.9879518072289, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1790195, Length=419, Percent_Identity=26.9689737470167, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1788392, Length=419, Percent_Identity=27.6849642004773, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323735, Length=343, Percent_Identity=25.3644314868805, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=283, Percent_Identity=24.3816254416961, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YCNB_BACSU (P94422)

Other databases:

- EMBL:   D50453
- EMBL:   AL009126
- PIR:   F69763
- RefSeq:   NP_388266.1
- ProteinModelPortal:   P94422
- EnsemblBacteria:   EBBACT00000001704
- GeneID:   938275
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU03840
- NMPDR:   fig|224308.1.peg.385
- GenoList:   BSU03840
- GeneTree:   EBGT00070000031768
- HOGENOM:   HBG679341
- OMA:   GTINTRT
- ProtClustDB:   CLSK886702
- BioCyc:   BSUB:BSU03840-MONOMER
- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411
- PRINTS:   PR01036
- TIGRFAMs:   TIGR00711

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 50799; Mature: 50799

Theoretical pI: Translated: 11.03; Mature: 11.03

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xc9615a4)-; HASH(0xc5cbff8)-; HASH(0xc62ca90)-; HASH(0xc8bb964)-; HASH(0xc60b5fc)-; HASH(0xc96994c)-; HASH(0xc62bf48)-; HASH(0xc6fa8b0)-; HASH(0x40a8bbfc)-; HASH(0xcb39364)-; HASH(0xbd704a4)-; HASH(0xcd38288)-; HASH(0xcd55e14)-; HASH(0xcd413c4)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSIEQKPFNRSVIVGILLAGAFVAILNQTLLITALPHIMRDFNVDANQAQWLTTSFML
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
TNGILIPITAFLIEKFTSRALLITAMSIFTAGTVVGAFAPNFPVLLTARIIQAAGAGIMM
HCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHH
PLMQTVFLTIFPIEKRGQAMGMVGLVISFAPAIGPTLSGWAVEAFSWRSLFYIILPFAVI
HHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DLILASILMKNVTTLRKTQIDILSVILSTFGFGGLLYGFSSVGSYGWSSSTVLISLLVGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
IALLLFITRQMKLKKPMLEFRVFTFGVFSLTTLLGTLVFALLIGTETILPLYTQNVRDVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHH
AFDTGLMLLPGAVVMGFMSPIIGRIFDRVGGRGLAIAGFCIIFLTSLPFMQLTDHTSLAW
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHCCCCCHHHH
IVVLYTVRLLGTAMIMMPVTTAGINALPRHLIPHGTAMNNTIRQVGGSIGTALLVSVMSN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
QAAHAGTTNVKHAALHGMNAAFIVAAVIALVGFLLSFTLKKPQRPAEQQPAR
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
>Mature Secondary Structure
MNTSIEQKPFNRSVIVGILLAGAFVAILNQTLLITALPHIMRDFNVDANQAQWLTTSFML
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
TNGILIPITAFLIEKFTSRALLITAMSIFTAGTVVGAFAPNFPVLLTARIIQAAGAGIMM
HCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHH
PLMQTVFLTIFPIEKRGQAMGMVGLVISFAPAIGPTLSGWAVEAFSWRSLFYIILPFAVI
HHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DLILASILMKNVTTLRKTQIDILSVILSTFGFGGLLYGFSSVGSYGWSSSTVLISLLVGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
IALLLFITRQMKLKKPMLEFRVFTFGVFSLTTLLGTLVFALLIGTETILPLYTQNVRDVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHH
AFDTGLMLLPGAVVMGFMSPIIGRIFDRVGGRGLAIAGFCIIFLTSLPFMQLTDHTSLAW
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHCCCCCHHHH
IVVLYTVRLLGTAMIMMPVTTAGINALPRHLIPHGTAMNNTIRQVGGSIGTALLVSVMSN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
QAAHAGTTNVKHAALHGMNAAFIVAAVIALVGFLLSFTLKKPQRPAEQQPAR
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377