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The map label for this gene is yckB

Identifier: 16077407

GI number: 16077407

Start: 367995

End: 368858

Strand: Reverse

Name: yckB

Synonym: BSU03380

Alternate gene names: 16077407

Gene position: 368858-367995 (Counterclockwise)

Preceding gene: 16077411

Following gene: 16077406

Centisome position: 8.75

GC content: 45.14

Gene sequence:

>864_bases
GTGAAATCATTCATGCATTCCAAAGCCGTTATATTCAGTTTCACAATGGCTTTCTTTCTTATATTGGCAGCCTGTTCCGG
CAAAAATGAAGCCGATTCCAAGGATACAGGCTGGGAGCAGATTAAGGATAAAGGGAAAATCGTAGTCGCTACATCAGGTA
CGCTCTATCCCACTTCTTATCACGATACTGACTCTGGCTCTGATAAGCTGACGGGCTACGAGGTTGAAGTTGTTCGTGAA
GCGGCAAAACGCTTGGGGCTGAAGGTTGAGTTCAAGGAGATGGGCATTGACGGGATGCTGACAGCGGTGAACAGCGGACA
GGTTGACGCAGCCGCGAATGACATCGATGTGACGAAGGACCGTGAGGAGAAGTTCGCATTTTCCACTCCATATAAATATT
CATACGGCACGGCAATTGTCCGAAAAGACGATTTATCAGGGATTAAAACGCTCAAGGATTTAAAAGGGAAGAAAGCGGCT
GGCGCGGCTACAACCGTCTACATGGAGGTCGCGAGAAAATACGGCGCCAAAGAAGTGATTTATGATAATGCGACGAATGA
GCAATATTTAAAAGATGTTGCCAACGGACGCACTGACGTCATTTTAAACGACTATTACCTGCAGACACTTGCGCTCGCGG
CATTCCCGGATCTCAACATTACAATTCATCCTGACATCAAGTACATGCCGAACAAACAGGCCCTTGTCATGAAAAAAAGC
AATGCGGCACTTCAGAAAAAGATGAACGAAGCATTGAAAGAGATGAGCAAGGATGGCTCTTTGACTAAACTGTCGAAACA
ATTCTTCAATAAAGCAGATGTATCAAAAAAAATTGATGCCGACGTTCAGGACGTTGATTTGTAA

Upstream 100 bases:

>100_bases
TATACACAATTCGAACTTTTTGAAAACGCGATCAACATATTGCAACCCCTTATGTTCAATGCTATCTTTTTAGTTGATTA
ACAGATTGGGGGAACTATAG

Downstream 100 bases:

>100_bases
TCGGACGCCATGATCAATAGCATTCAGTGGGAATATATTTTCAACACGAAGCTGGCCATTGAATCGTTCCCTTATGTCAT
AAAAGGCATTGGTTATACAC

Product: ABC transporter binding lipoprotein

Products: NA

Alternate protein names: ORF2

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MKSFMHSKAVIFSFTMAFFLILAACSGKNEADSKDTGWEQIKDKGKIVVATSGTLYPTSYHDTDSGSDKLTGYEVEVVRE
AAKRLGLKVEFKEMGIDGMLTAVNSGQVDAAANDIDVTKDREEKFAFSTPYKYSYGTAIVRKDDLSGIKTLKDLKGKKAA
GAATTVYMEVARKYGAKEVIYDNATNEQYLKDVANGRTDVILNDYYLQTLALAAFPDLNITIHPDIKYMPNKQALVMKKS
NAALQKKMNEALKEMSKDGSLTKLSKQFFNKADVSKKIDADVQDVDL

Sequences:

>Translated_287_residues
MKSFMHSKAVIFSFTMAFFLILAACSGKNEADSKDTGWEQIKDKGKIVVATSGTLYPTSYHDTDSGSDKLTGYEVEVVRE
AAKRLGLKVEFKEMGIDGMLTAVNSGQVDAAANDIDVTKDREEKFAFSTPYKYSYGTAIVRKDDLSGIKTLKDLKGKKAA
GAATTVYMEVARKYGAKEVIYDNATNEQYLKDVANGRTDVILNDYYLQTLALAAFPDLNITIHPDIKYMPNKQALVMKKS
NAALQKKMNEALKEMSKDGSLTKLSKQFFNKADVSKKIDADVQDVDL
>Mature_287_residues
MKSFMHSKAVIFSFTMAFFLILAACSGKNEADSKDTGWEQIKDKGKIVVATSGTLYPTSYHDTDSGSDKLTGYEVEVVRE
AAKRLGLKVEFKEMGIDGMLTAVNSGQVDAAANDIDVTKDREEKFAFSTPYKYSYGTAIVRKDDLSGIKTLKDLKGKKAA
GAATTVYMEVARKYGAKEVIYDNATNEQYLKDVANGRTDVILNDYYLQTLALAAFPDLNITIHPDIKYMPNKQALVMKKS
NAALQKKMNEALKEMSKDGSLTKLSKQFFNKADVSKKIDADVQDVDL

Specific function: Probably part of a binding-protein-dependent transport system

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family

Homologues:

Organism=Escherichia coli, GI1788228, Length=239, Percent_Identity=34.7280334728033, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787085, Length=228, Percent_Identity=25.4385964912281, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787031, Length=229, Percent_Identity=24.8908296943231, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787088, Length=223, Percent_Identity=24.2152466367713, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788649, Length=234, Percent_Identity=24.7863247863248, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): YCKB_BACSU (P42400)

Other databases:

- EMBL:   D30762
- EMBL:   AL009126
- PIR:   D69760
- RefSeq:   NP_388220.1
- PDB:   2IEE
- PDBsum:   2IEE
- ProteinModelPortal:   P42400
- SMR:   P42400
- EnsemblBacteria:   EBBACT00000003943
- GeneID:   938320
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU03380
- NMPDR:   fig|224308.1.peg.339
- GenoList:   BSU03380
- GeneTree:   EBGT00050000000372
- HOGENOM:   HBG698334
- OMA:   PDYELAF
- ProtClustDB:   CLSK2752308
- BioCyc:   BSUB:BSU03380-MONOMER
- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313
- PANTHER:   PTHR18966
- SMART:   SM00062

Pfam domain/function: PF00497 SBP_bac_3

EC number: NA

Molecular weight: Translated: 31720; Mature: 31720

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS01039 SBP_BACTERIAL_3; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSFMHSKAVIFSFTMAFFLILAACSGKNEADSKDTGWEQIKDKGKIVVATSGTLYPTSY
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCEEEEECCEECCCCC
HDTDSGSDKLTGYEVEVVREAAKRLGLKVEFKEMGIDGMLTAVNSGQVDAAANDIDVTKD
CCCCCCCCCCCCHHHHHHHHHHHHCCCEEEHHHCCCCEEEEEECCCCCCCCCCCCCCCCC
REEKFAFSTPYKYSYGTAIVRKDDLSGIKTLKDLKGKKAAGAATTVYMEVARKYGAKEVI
CHHHEEECCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEE
YDNATNEQYLKDVANGRTDVILNDYYLQTLALAAFPDLNITIHPDIKYMPNKQALVMKKS
ECCCCCHHHHHHHHCCCCEEEECHHHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEECC
NAALQKKMNEALKEMSKDGSLTKLSKQFFNKADVSKKIDADVQDVDL
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHCCCCCHHCCC
>Mature Secondary Structure
MKSFMHSKAVIFSFTMAFFLILAACSGKNEADSKDTGWEQIKDKGKIVVATSGTLYPTSY
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCEEEEECCEECCCCC
HDTDSGSDKLTGYEVEVVREAAKRLGLKVEFKEMGIDGMLTAVNSGQVDAAANDIDVTKD
CCCCCCCCCCCCHHHHHHHHHHHHCCCEEEHHHCCCCEEEEEECCCCCCCCCCCCCCCCC
REEKFAFSTPYKYSYGTAIVRKDDLSGIKTLKDLKGKKAAGAATTVYMEVARKYGAKEVI
CHHHEEECCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEE
YDNATNEQYLKDVANGRTDVILNDYYLQTLALAAFPDLNITIHPDIKYMPNKQALVMKKS
ECCCCCHHHHHHHHCCCCEEEECHHHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEECC
NAALQKKMNEALKEMSKDGSLTKLSKQFFNKADVSKKIDADVQDVDL
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7704255; 9384377