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The map label for this gene is ycgS

Identifier: 16077395

GI number: 16077395

Start: 351842

End: 352696

Strand: Reverse

Name: ycgS

Synonym: BSU03260

Alternate gene names: 16077395

Gene position: 352696-351842 (Counterclockwise)

Preceding gene: 16077397

Following gene: 16077394

Centisome position: 8.37

GC content: 46.43

Gene sequence:

>855_bases
ATGAAAAGACGAGTTGAAACGATCACCTTCGATGGCGGTACACTAGAATATTCTGTGACAGGCAAGGGAACTCCGATCCT
TGTCATGCATGGCGGGCATTCAAATTGTTACGAGGAATTCGGATACACAGCGTTGATTGAACAAGGGTATTCCATCATTA
CGCCTTCAAGGCCTGGCTATGGACGGACGTCAAAAGAAATTGGAAAAAGTCTTGCCAATGCCTGCCGTTTCTATGTGAAA
TTATTAGATCATCTACAAATCGAGAGTGTCCATGTGATCGCCATCTCAGCAGGAGGGCCGAGCGGCATATGCTTTGCCTC
ACATTATCCGGAAAGAGTAAATACATTGACCCTGCAATCCGCAGTCACCAAAGAGTGGCTCACCCCAAAAGACACTGAAT
ATAAATTGGGGGAAATTCTCTTCCGCCCGCCGGTGGAAAAGTGGATATGGAAGCTGATTTCTTCCTTGAATAACGCTTTC
CCGCGTTTGATGTTTCGAGCTATGAGCCCCCAATTCAGCACGCTTCCGTTTCAGCGAATCAAGTCCTTGATGAATGAAAA
AGACATAGAAGCATTTCGGAAAATGAACAGCCGGCAGCGCTCGGGTGAAGGATTTCTGATTGACTTGTCACAAACCGCCG
CTGTCTCTTTAAAAGACCTGCAAGCCATCATATGTCCCGTGCTGATTATGCAAAGTGTTTATGACGGATTGGTTGACTTG
TCACATGCACATCATGCAAAAGAGCACATTCGTGGCGCTGTTCTCTGTTTGCTTCACTCATGGGGACACCTGATTTGGCT
CGGAAAAGAAGCTGCAGAAACAGGCAGTATCCTACTCGGCTTTTTGGAATCCTAA

Upstream 100 bases:

>100_bases
AGTATATCATATTTCAAGGGCCTTTGTTTATTCATTTCTTCTACCTTGTGGTCAATTGCTGAAAACTCTATAATAGGCAA
AAACCAATTGGGTGAAACAG

Downstream 100 bases:

>100_bases
CCAAAGTGAAACGATCCTTTCCCCTCCTCCGTCAAACAAGCAGTGCCTTTTTTTCTGGTATCATAAGAAAGGCAGCTTAA
CATGAGCGGAGGTTTTATTG

Product: aromatic hydrocarbon hydrolase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MKRRVETITFDGGTLEYSVTGKGTPILVMHGGHSNCYEEFGYTALIEQGYSIITPSRPGYGRTSKEIGKSLANACRFYVK
LLDHLQIESVHVIAISAGGPSGICFASHYPERVNTLTLQSAVTKEWLTPKDTEYKLGEILFRPPVEKWIWKLISSLNNAF
PRLMFRAMSPQFSTLPFQRIKSLMNEKDIEAFRKMNSRQRSGEGFLIDLSQTAAVSLKDLQAIICPVLIMQSVYDGLVDL
SHAHHAKEHIRGAVLCLLHSWGHLIWLGKEAAETGSILLGFLES

Sequences:

>Translated_284_residues
MKRRVETITFDGGTLEYSVTGKGTPILVMHGGHSNCYEEFGYTALIEQGYSIITPSRPGYGRTSKEIGKSLANACRFYVK
LLDHLQIESVHVIAISAGGPSGICFASHYPERVNTLTLQSAVTKEWLTPKDTEYKLGEILFRPPVEKWIWKLISSLNNAF
PRLMFRAMSPQFSTLPFQRIKSLMNEKDIEAFRKMNSRQRSGEGFLIDLSQTAAVSLKDLQAIICPVLIMQSVYDGLVDL
SHAHHAKEHIRGAVLCLLHSWGHLIWLGKEAAETGSILLGFLES
>Mature_284_residues
MKRRVETITFDGGTLEYSVTGKGTPILVMHGGHSNCYEEFGYTALIEQGYSIITPSRPGYGRTSKEIGKSLANACRFYVK
LLDHLQIESVHVIAISAGGPSGICFASHYPERVNTLTLQSAVTKEWLTPKDTEYKLGEILFRPPVEKWIWKLISSLNNAF
PRLMFRAMSPQFSTLPFQRIKSLMNEKDIEAFRKMNSRQRSGEGFLIDLSQTAAVSLKDLQAIICPVLIMQSVYDGLVDL
SHAHHAKEHIRGAVLCLLHSWGHLIWLGKEAAETGSILLGFLES

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YCGS_BACSU (P94396)

Other databases:

- EMBL:   D50453
- EMBL:   AL009126
- PIR:   F69759
- RefSeq:   NP_388208.1
- ProteinModelPortal:   P94396
- SMR:   P94396
- EnsemblBacteria:   EBBACT00000004053
- GeneID:   938332
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU03260
- GenoList:   BSU03260
- GeneTree:   EBGT00050000000449
- HOGENOM:   HBG534375
- OMA:   HAKEHIR
- ProtClustDB:   CLSK249496
- BioCyc:   BSUB:BSU03260-MONOMER
- InterPro:   IPR000073
- PRINTS:   PR00111

Pfam domain/function: PF00561 Abhydrolase_1

EC number: 3.7.1.- [C]

Molecular weight: Translated: 31740; Mature: 31740

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: ACT_SITE 96-96

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRVETITFDGGTLEYSVTGKGTPILVMHGGHSNCYEEFGYTALIEQGYSIITPSRPGY
CCCEEEEEEECCCEEEEEEECCCCEEEEEECCCCHHHHHHCHHHHHHCCCEEECCCCCCC
GRTSKEIGKSLANACRFYVKLLDHLQIESVHVIAISAGGPSGICFASHYPERVNTLTLQS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEECCCCCHHHHHHHHHH
AVTKEWLTPKDTEYKLGEILFRPPVEKWIWKLISSLNNAFPRLMFRAMSPQFSTLPFQRI
HHHHHCCCCCCCCHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
KSLMNEKDIEAFRKMNSRQRSGEGFLIDLSQTAAVSLKDLQAIICPVLIMQSVYDGLVDL
HHHHCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SHAHHAKEHIRGAVLCLLHSWGHLIWLGKEAAETGSILLGFLES
HHHHHHHHHHHHHHHHHHHHCCCEEEECCHHCCCCHHHHEEECC
>Mature Secondary Structure
MKRRVETITFDGGTLEYSVTGKGTPILVMHGGHSNCYEEFGYTALIEQGYSIITPSRPGY
CCCEEEEEEECCCEEEEEEECCCCEEEEEECCCCHHHHHHCHHHHHHCCCEEECCCCCCC
GRTSKEIGKSLANACRFYVKLLDHLQIESVHVIAISAGGPSGICFASHYPERVNTLTLQS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEECCCCCHHHHHHHHHH
AVTKEWLTPKDTEYKLGEILFRPPVEKWIWKLISSLNNAFPRLMFRAMSPQFSTLPFQRI
HHHHHCCCCCCCCHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
KSLMNEKDIEAFRKMNSRQRSGEGFLIDLSQTAAVSLKDLQAIICPVLIMQSVYDGLVDL
HHHHCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SHAHHAKEHIRGAVLCLLHSWGHLIWLGKEAAETGSILLGFLES
HHHHHHHHHHHHHHHHHHHHCCCEEEECCHHCCCCHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377