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The map label for this gene is pcp

Identifier: 16077334

GI number: 16077334

Start: 286773

End: 287420

Strand: Reverse

Name: pcp

Synonym: BSU02650

Alternate gene names: 16077334

Gene position: 287420-286773 (Counterclockwise)

Preceding gene: 16077336

Following gene: 16077328

Centisome position: 6.82

GC content: 50.0

Gene sequence:

>648_bases
ATGAGAAAAAAAGTGCTGATCACAGGCTTTGACCCTTTTGACAAAGAAACCGTCAATCCATCATGGGAAGCGGCGAAACG
GCTTAATGGCTTCGAGACAGAAGAAGCCATTATTACAGCGGAACAAATTCCAACCGTCTTTAGATCCGCTCTGGACACTC
TGCGCCAAGCCATTCAAAAACATCAGCCAGATATCGTAATTTGTGTCGGCCAAGCAGGAGGACGGATGCAGATTACACCG
GAACGAGTGGCAATCAACCTTGCAGATGCGCGAATCCCCGATAACGAAGGACATCAGCCGATTGATGAAGAGATTTCTCC
AGATGGGCCCGCCGCTTACTGGACAAGGCTTCCCGTGAAACGAATGACTGCTAAGATGAAGGAACACGGCATTCCAGCTG
CGGTTTCCTACACAGCGGGGACCTTTGTATGCAACTATTTGTTCTACGGGTTAATGGATCACATTAGCCGGACATCCCCA
CACATTCGCGGCGGTTTTATTCATATTCCTTACATTCCGCAGCAAACAATCGACAAAACAGCGCCGAGCCTCAGCCTGGA
CACGATTGTCCGGGCATTGAGAATCGCCGCTGTTACGGCCGCACAATATGATGAGGATGTGAAGTCACCGGGTGGTACGC
TGCACTAA

Upstream 100 bases:

>100_bases
ACTGCTCCGGCAAATGCTCCATTATGATTCACTCCTTTACATTATTTTGATGAAAAACCCATAAATAAACAATAGAATAA
AAGAAAAAGGAGATGGGAGA

Downstream 100 bases:

>100_bases
GCGGCCGCCGCTGTTTCTTCCCTCATCCTCTTTTTGTAGACAAACGCCGATAGGGTGACACTCACTTCAAACAGGACAAG
AAGCGGGATCATCACGAGAA

Product: pyrrolidone-carboxylate peptidase

Products: NA

Alternate protein names: 5-oxoprolyl-peptidase; Pyroglutamyl-peptidase I; PGP-I; Pyrase

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MRKKVLITGFDPFDKETVNPSWEAAKRLNGFETEEAIITAEQIPTVFRSALDTLRQAIQKHQPDIVICVGQAGGRMQITP
ERVAINLADARIPDNEGHQPIDEEISPDGPAAYWTRLPVKRMTAKMKEHGIPAAVSYTAGTFVCNYLFYGLMDHISRTSP
HIRGGFIHIPYIPQQTIDKTAPSLSLDTIVRALRIAAVTAAQYDEDVKSPGGTLH

Sequences:

>Translated_215_residues
MRKKVLITGFDPFDKETVNPSWEAAKRLNGFETEEAIITAEQIPTVFRSALDTLRQAIQKHQPDIVICVGQAGGRMQITP
ERVAINLADARIPDNEGHQPIDEEISPDGPAAYWTRLPVKRMTAKMKEHGIPAAVSYTAGTFVCNYLFYGLMDHISRTSP
HIRGGFIHIPYIPQQTIDKTAPSLSLDTIVRALRIAAVTAAQYDEDVKSPGGTLH
>Mature_215_residues
MRKKVLITGFDPFDKETVNPSWEAAKRLNGFETEEAIITAEQIPTVFRSALDTLRQAIQKHQPDIVICVGQAGGRMQITP
ERVAINLADARIPDNEGHQPIDEEISPDGPAAYWTRLPVKRMTAKMKEHGIPAAVSYTAGTFVCNYLFYGLMDHISRTSP
HIRGGFIHIPYIPQQTIDKTAPSLSLDTIVRALRIAAVTAAQYDEDVKSPGGTLH

Specific function: Removes 5-oxoproline from various penultimate amino acid residues except L-proline

COG id: COG2039

COG function: function code O; Pyrrolidone-carboxylate peptidase (N-terminal pyroglutamyl peptidase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C15 family

Homologues:

Organism=Homo sapiens, GI8923198, Length=172, Percent_Identity=26.7441860465116, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PCP_BACSU (P28618)

Other databases:

- EMBL:   X66034
- EMBL:   D30808
- EMBL:   AL009126
- PIR:   S23432
- RefSeq:   NP_388147.1
- ProteinModelPortal:   P28618
- SMR:   P28618
- MEROPS:   C15.001
- EnsemblBacteria:   EBBACT00000004041
- GeneID:   938396
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU02650
- NMPDR:   fig|224308.1.peg.266
- GenoList:   BSU02650
- GeneTree:   EBGT00050000001251
- HOGENOM:   HBG360405
- OMA:   NTAGTYV
- ProtClustDB:   PRK13197
- BioCyc:   BSUB:BSU02650-MONOMER
- BRENDA:   3.4.19.3
- GO:   GO:0005737
- GO:   GO:0006508
- HAMAP:   MF_00417
- InterPro:   IPR000816
- InterPro:   IPR016125
- Gene3D:   G3DSA:3.40.630.20
- PANTHER:   PTHR23402
- PIRSF:   PIRSF015592
- PRINTS:   PR00706
- TIGRFAMs:   TIGR00504

Pfam domain/function: PF01470 Peptidase_C15; SSF53182 Peptidase_C15-like

EC number: =3.4.19.3

Molecular weight: Translated: 23774; Mature: 23774

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS01334 PYRASE_CYS; PS01333 PYRASE_GLU

Important sites: ACT_SITE 81-81 ACT_SITE 144-144 ACT_SITE 168-168

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKKVLITGFDPFDKETVNPSWEAAKRLNGFETEEAIITAEQIPTVFRSALDTLRQAIQK
CCCEEEEECCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
HQPDIVICVGQAGGRMQITPERVAINLADARIPDNEGHQPIDEEISPDGPAAYWTRLPVK
CCCCEEEEECCCCCEEEECHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHH
RMTAKMKEHGIPAAVSYTAGTFVCNYLFYGLMDHISRTSPHIRGGFIHIPYIPQQTIDKT
HHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHC
APSLSLDTIVRALRIAAVTAAQYDEDVKSPGGTLH
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRKKVLITGFDPFDKETVNPSWEAAKRLNGFETEEAIITAEQIPTVFRSALDTLRQAIQK
CCCEEEEECCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
HQPDIVICVGQAGGRMQITPERVAINLADARIPDNEGHQPIDEEISPDGPAAYWTRLPVK
CCCCEEEEECCCCCEEEECHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHH
RMTAKMKEHGIPAAVSYTAGTFVCNYLFYGLMDHISRTSPHIRGGFIHIPYIPQQTIDKT
HHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHC
APSLSLDTIVRALRIAAVTAAQYDEDVKSPGGTLH
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1353026; 7704254; 9384377; 1362573