Click here to switch to the map view.

The map label for this gene is lmrB

Identifier: 16077336

GI number: 16077336

Start: 288653

End: 290092

Strand: Reverse

Name: lmrB

Synonym: BSU02670

Alternate gene names: 16077336

Gene position: 290092-288653 (Counterclockwise)

Preceding gene: 16077337

Following gene: 16077334

Centisome position: 6.88

GC content: 47.36

Gene sequence:

>1440_bases
ATGATTTTGGAAACAACAGCTAAAGCATCTCAGCAATACAAAGTGATGCCGATTATGATTTCCTTGCTGTTGGCCGGTTT
TATCGGCATGTTCAGTGAAACAGCGCTGAATATTGCGTTAACCGACCTTATGAAGGAATTGAACATTACAGCGGCAACCG
TCCAATGGTTAACGACGGGCTACCTGCTTGTACTCGGTATCCTTGTTCCTGTTTCAGGACTGCTGTTGCAGTGGTTCACA
ACAAGACAGCTTTTTACCGTGTCACTTATCTTTTCAATTTTAGGTACATTCATTGCGGCGCTTGCGCCGAGCTTCTCGTT
TTTATTAGCGGCAAGGATCGTTCAGGCACTTGGAACCGGTCTTTTACTGCCGCTGATGTTTAACACAATCTTGGTGATTT
TCCCGCCTCATAAACGGGGTGCCGCAATGGGAACGATCGGGCTTGTCATTATGTTCGCGCCCGCCATCGGCCCAACTTTC
TCAGGATTGGTTCTGGAGCATCTCAACTGGCACTGGATTTTCTGGATCTCTCTTCCATTCCTTGTGCTGGCGCTTGTTTT
CGGTATCGCATATATGCAAAATGTATCTGAAACGACAAAGCCGAAAATCGATGTATTGTCTATCATCCTGTCGACGATTG
GCTTCGGCGGCATTGTATTCGGATTCAGCAACGCGGGTGAAGGCTCCGGGGGATGGTCCAGCCCGACTGTTATCGTGTCG
CTGATTGTCGGCGTTGTCGGCCTTATCTTATTTTCAATCCGCCAGCTGACAATGAAGCAGCCTATGATGAACCTCCGTGC
GTTCAAATACCCGATGTTTATTTTGGGTGTGATCATGGTGTTCATTTGTATGATGGTCATTCTGTCATCTATGCTGCTTC
TGCCGATGTATCTGCAAGGCGGCTTAGTCCTCACTGCATTTGCATCTGGTCTTGTTCTCTTGCCGGGCGGTATTTTAAAT
GGATTTATGTCCCCTGTTACAGGCCGCTTGTTCGATAAATACGGGCCGAAATGGCTTGTCATTCCGGGATTTGTGATTGT
CACCGTTGTACTATGGTTCTTCTCAAATGTCACGACCACTTCAACAGCTGTGCTGATTATCATCCTGCACACCTGCTTGA
TGATCGGGATTTCTATGATCATGATGCCTGCACAGACAAACGGTTTAAACCAACTGCCGCGTGAATTTTATCCAGACGGC
ACCGCCATTATGAACACGCTGCAGCAAATGGCCGGCGCTATCGGAACAGCGGTTGCGGTCAGCATTATGGCTGCAGGCCA
GCATGATTATATGAGTACAGTTAAAAACCCTGCCGATCCGGCAGTCATCCCGCAAGCTTTGACAGCAGGCGTACAGCACG
CATTTGTGTTTGCAATGATTGTTGCCATTATCGGTTTAATTGGCGCTTTCTTTATGAAACGCGTTAAAGTAGATCATTAA

Upstream 100 bases:

>100_bases
AAAAAACAGTACGCCGCTCCTTCATATCTCCAGCTGCATTCCTGACCTGCTGAAGAGATAAAAAAAACGACATACTACAT
GCAGTTGATAGAGAGGGGAA

Downstream 100 bases:

>100_bases
TATGAAAAGCCCCTGACTAGTGTTCAGGGGCTTTTTCATGTTTACTTGTGGATCGTTTTCGCCGCGATTTCATCAATCGC
GTGCCGCATTTCCTCCTTCG

Product: efflux transporter; drug-export protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MILETTAKASQQYKVMPIMISLLLAGFIGMFSETALNIALTDLMKELNITAATVQWLTTGYLLVLGILVPVSGLLLQWFT
TRQLFTVSLIFSILGTFIAALAPSFSFLLAARIVQALGTGLLLPLMFNTILVIFPPHKRGAAMGTIGLVIMFAPAIGPTF
SGLVLEHLNWHWIFWISLPFLVLALVFGIAYMQNVSETTKPKIDVLSIILSTIGFGGIVFGFSNAGEGSGGWSSPTVIVS
LIVGVVGLILFSIRQLTMKQPMMNLRAFKYPMFILGVIMVFICMMVILSSMLLLPMYLQGGLVLTAFASGLVLLPGGILN
GFMSPVTGRLFDKYGPKWLVIPGFVIVTVVLWFFSNVTTTSTAVLIIILHTCLMIGISMIMMPAQTNGLNQLPREFYPDG
TAIMNTLQQMAGAIGTAVAVSIMAAGQHDYMSTVKNPADPAVIPQALTAGVQHAFVFAMIVAIIGLIGAFFMKRVKVDH

Sequences:

>Translated_479_residues
MILETTAKASQQYKVMPIMISLLLAGFIGMFSETALNIALTDLMKELNITAATVQWLTTGYLLVLGILVPVSGLLLQWFT
TRQLFTVSLIFSILGTFIAALAPSFSFLLAARIVQALGTGLLLPLMFNTILVIFPPHKRGAAMGTIGLVIMFAPAIGPTF
SGLVLEHLNWHWIFWISLPFLVLALVFGIAYMQNVSETTKPKIDVLSIILSTIGFGGIVFGFSNAGEGSGGWSSPTVIVS
LIVGVVGLILFSIRQLTMKQPMMNLRAFKYPMFILGVIMVFICMMVILSSMLLLPMYLQGGLVLTAFASGLVLLPGGILN
GFMSPVTGRLFDKYGPKWLVIPGFVIVTVVLWFFSNVTTTSTAVLIIILHTCLMIGISMIMMPAQTNGLNQLPREFYPDG
TAIMNTLQQMAGAIGTAVAVSIMAAGQHDYMSTVKNPADPAVIPQALTAGVQHAFVFAMIVAIIGLIGAFFMKRVKVDH
>Mature_479_residues
MILETTAKASQQYKVMPIMISLLLAGFIGMFSETALNIALTDLMKELNITAATVQWLTTGYLLVLGILVPVSGLLLQWFT
TRQLFTVSLIFSILGTFIAALAPSFSFLLAARIVQALGTGLLLPLMFNTILVIFPPHKRGAAMGTIGLVIMFAPAIGPTF
SGLVLEHLNWHWIFWISLPFLVLALVFGIAYMQNVSETTKPKIDVLSIILSTIGFGGIVFGFSNAGEGSGGWSSPTVIVS
LIVGVVGLILFSIRQLTMKQPMMNLRAFKYPMFILGVIMVFICMMVILSSMLLLPMYLQGGLVLTAFASGLVLLPGGILN
GFMSPVTGRLFDKYGPKWLVIPGFVIVTVVLWFFSNVTTTSTAVLIIILHTCLMIGISMIMMPAQTNGLNQLPREFYPDG
TAIMNTLQQMAGAIGTAVAVSIMAAGQHDYMSTVKNPADPAVIPQALTAGVQHAFVFAMIVAIIGLIGAFFMKRVKVDH

Specific function: Proton-dependent transporter. May mediate the efflux of lincomycin

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family

Homologues:

Organism=Escherichia coli, GI1788710, Length=425, Percent_Identity=25.4117647058824, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1789042, Length=430, Percent_Identity=25.3488372093023, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI87081983, Length=383, Percent_Identity=24.5430809399478, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1790195, Length=406, Percent_Identity=24.6305418719212, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1788392, Length=398, Percent_Identity=25.6281407035176, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6325455, Length=232, Percent_Identity=26.7241379310345, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LMRB_BACSU (O35018)

Other databases:

- EMBL:   AB000617
- EMBL:   AL009126
- PIR:   E69652
- RefSeq:   NP_388149.1
- ProteinModelPortal:   O35018
- EnsemblBacteria:   EBBACT00000000482
- GeneID:   938388
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU02670
- NMPDR:   fig|224308.1.peg.268
- GenoList:   BSU02670
- GeneTree:   EBGT00070000031768
- HOGENOM:   HBG679341
- OMA:   GENGWGS
- ProtClustDB:   CLSK886630
- BioCyc:   BSUB:BSU02670-MONOMER
- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411
- PRINTS:   PR01036
- TIGRFAMs:   TIGR00711

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 51710; Mature: 51710

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xc9f44c8)-; HASH(0xb294a58)-; HASH(0xc3d9688)-; HASH(0xc90e288)-; HASH(0xb38ee34)-; HASH(0xbd8ceac)-; HASH(0xc3d9880)-; HASH(0xcb24450)-; HASH(0xb3dc3fc)-; HASH(0xc82ec48)-; HASH(0xcae402c)-; HASH(0xcab087c)-; HASH(0xbd94a04)-; HASH(0xc84b498)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILETTAKASQQYKVMPIMISLLLAGFIGMFSETALNIALTDLMKELNITAATVQWLTTG
CCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
YLLVLGILVPVSGLLLQWFTTRQLFTVSLIFSILGTFIAALAPSFSFLLAARIVQALGTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLPLMFNTILVIFPPHKRGAAMGTIGLVIMFAPAIGPTFSGLVLEHLNWHWIFWISLPF
HHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHH
LVLALVFGIAYMQNVSETTKPKIDVLSIILSTIGFGGIVFGFSNAGEGSGGWSSPTVIVS
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCHHHHHH
LIVGVVGLILFSIRQLTMKQPMMNLRAFKYPMFILGVIMVFICMMVILSSMLLLPMYLQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLVLTAFASGLVLLPGGILNGFMSPVTGRLFDKYGPKWLVIPGFVIVTVVLWFFSNVTTT
CHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHCCCHH
STAVLIIILHTCLMIGISMIMMPAQTNGLNQLPREFYPDGTAIMNTLQQMAGAIGTAVAV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHH
SIMAAGQHDYMSTVKNPADPAVIPQALTAGVQHAFVFAMIVAIIGLIGAFFMKRVKVDH
HHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
>Mature Secondary Structure
MILETTAKASQQYKVMPIMISLLLAGFIGMFSETALNIALTDLMKELNITAATVQWLTTG
CCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
YLLVLGILVPVSGLLLQWFTTRQLFTVSLIFSILGTFIAALAPSFSFLLAARIVQALGTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLPLMFNTILVIFPPHKRGAAMGTIGLVIMFAPAIGPTFSGLVLEHLNWHWIFWISLPF
HHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHH
LVLALVFGIAYMQNVSETTKPKIDVLSIILSTIGFGGIVFGFSNAGEGSGGWSSPTVIVS
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCHHHHHH
LIVGVVGLILFSIRQLTMKQPMMNLRAFKYPMFILGVIMVFICMMVILSSMLLLPMYLQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLVLTAFASGLVLLPGGILNGFMSPVTGRLFDKYGPKWLVIPGFVIVTVVLWFFSNVTTT
CHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHCCCHH
STAVLIIILHTCLMIGISMIMMPAQTNGLNQLPREFYPDGTAIMNTLQQMAGAIGTAVAV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHH
SIMAAGQHDYMSTVKNPADPAVIPQALTAGVQHAFVFAMIVAIIGLIGAFFMKRVKVDH
HHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377