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The map label for this gene is alkA

Identifier: 16077248

GI number: 16077248

Start: 202547

End: 203458

Strand: Reverse

Name: alkA

Synonym: BSU01800

Alternate gene names: 16077248

Gene position: 203458-202547 (Counterclockwise)

Preceding gene: 16077272

Following gene: 255767046

Centisome position: 4.83

GC content: 36.95

Gene sequence:

>912_bases
GTGACATGGCATGAAGTCAATGATGTTATTGTCATTACATTACCTGAAATTTTCGACATGAATGCGAACCTTGGCTACCT
AACCAGGGAAAAAAATGAATGCATGTATGAAATAGAGAACAATATCATTACAAAAGTTATAGCCATTGGGGAAATTCGGT
CCTTAGTGCAGGTAAGCGTAATCAATAATAAACAAATGATTGTTCAATTCCTAAACGATTCTAGACCTGTTGAGCAGTGG
AAACGGGAAGAGATTGTAAAATATATTCATGAATGGTTTGATCTTGATAACGATTTAACTCCATTTTATGAAATGGCAAA
AGCAGATCCATTACTTAAAATGCCTGCCCGAAAATTTTATGGGTTGCGAGTGATCGGCATTCCCGATTTATTTGAAGCTT
TATGTTGGGGAGTTTTAGGGCAACAAATTAACTTAGCCTTCGCGTACTCCTTAAAGAAGCAATTTGTAGAAGCATTTGGC
GATTCTATTGAATGGAATGGTAAAAAGTATTGGGTGTTCCCACCGTACGAGCGAATTGCACGGTTAACCCCTACCGACCT
GGCAGATATTAAAATGACGGTGAAAAAAAGTGAATATATCATTGGAATAGCCAGATTAATGGCAAGTGGAGAATTATCGA
GGGAAAAATTAATGAAAATGAACTTTAAAGATGCTGAAAAAAACTTAATTAAAATACGAGGAATCGGTCCTTGGACAGCC
AATTATGTTCTAATGCGCTGCCTTAGGTTCCCGACAGCTTTTCCAATCGATGATGTGGGTCTTATTCATTCGATAAAAAT
ATTACGTAATATGAACCGAAAGCCTACGAAAGATGAAATCTTAGAAATATCGGTTCCATGGAAAGAATGGCAATCATACG
CTACCTTTTATTTATGGCGTGTCCTTTACTGA

Upstream 100 bases:

>100_bases
AATAGCAAGATAACAAAATGAGTAAAGATGATTATGTGATAAACTAATTTCAACCAGCGTGAGTTTTCTTATAATTATTT
AAGATAAGGAGAATGAAAAA

Downstream 100 bases:

>100_bases
ATCATTTCAACTTTAACCGGAATGTACTCAATAAAAAAAGAATGAACTTTAAAACCTAATCCCACAAGTACATTGTAAGT
GCGACTTCTAGAATTCTTTG

Product: DNA-3-methyladenine glycosylase

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosidase

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MTWHEVNDVIVITLPEIFDMNANLGYLTREKNECMYEIENNIITKVIAIGEIRSLVQVSVINNKQMIVQFLNDSRPVEQW
KREEIVKYIHEWFDLDNDLTPFYEMAKADPLLKMPARKFYGLRVIGIPDLFEALCWGVLGQQINLAFAYSLKKQFVEAFG
DSIEWNGKKYWVFPPYERIARLTPTDLADIKMTVKKSEYIIGIARLMASGELSREKLMKMNFKDAEKNLIKIRGIGPWTA
NYVLMRCLRFPTAFPIDDVGLIHSIKILRNMNRKPTKDEILEISVPWKEWQSYATFYLWRVLY

Sequences:

>Translated_303_residues
MTWHEVNDVIVITLPEIFDMNANLGYLTREKNECMYEIENNIITKVIAIGEIRSLVQVSVINNKQMIVQFLNDSRPVEQW
KREEIVKYIHEWFDLDNDLTPFYEMAKADPLLKMPARKFYGLRVIGIPDLFEALCWGVLGQQINLAFAYSLKKQFVEAFG
DSIEWNGKKYWVFPPYERIARLTPTDLADIKMTVKKSEYIIGIARLMASGELSREKLMKMNFKDAEKNLIKIRGIGPWTA
NYVLMRCLRFPTAFPIDDVGLIHSIKILRNMNRKPTKDEILEISVPWKEWQSYATFYLWRVLY
>Mature_302_residues
TWHEVNDVIVITLPEIFDMNANLGYLTREKNECMYEIENNIITKVIAIGEIRSLVQVSVINNKQMIVQFLNDSRPVEQWK
REEIVKYIHEWFDLDNDLTPFYEMAKADPLLKMPARKFYGLRVIGIPDLFEALCWGVLGQQINLAFAYSLKKQFVEAFGD
SIEWNGKKYWVFPPYERIARLTPTDLADIKMTVKKSEYIIGIARLMASGELSREKLMKMNFKDAEKNLIKIRGIGPWTAN
YVLMRCLRFPTAFPIDDVGLIHSIKILRNMNRKPTKDEILEISVPWKEWQSYATFYLWRVLY

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2- methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkylbase DNA glycosidase alkA family

Homologues:

Organism=Escherichia coli, GI1788383, Length=286, Percent_Identity=25.1748251748252, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): 3MGA_BACSU (P37878)

Other databases:

- EMBL:   D14465
- EMBL:   AB006424
- EMBL:   AL009126
- PIR:   E69584
- RefSeq:   NP_388061.1
- ProteinModelPortal:   P37878
- SMR:   P37878
- EnsemblBacteria:   EBBACT00000003321
- GeneID:   938586
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU01800
- NMPDR:   fig|224308.1.peg.180
- GenoList:   BSU01800
- GeneTree:   EBGT00050000002395
- HOGENOM:   HBG341234
- OMA:   KDFAANW
- PhylomeDB:   P37878
- ProtClustDB:   CLSK886592
- BioCyc:   BSUB:BSU01800-MONOMER
- BRENDA:   3.2.2.21
- InterPro:   IPR010316
- InterPro:   IPR000035
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR023170
- InterPro:   IPR012904
- Gene3D:   G3DSA:3.30.310.20
- Gene3D:   G3DSA:1.10.340.30
- Gene3D:   G3DSA:1.10.1670.10
- SMART:   SM00478

Pfam domain/function: PF00730 HhH-GPD; PF07934 OGG_N; SSF48150 DNA_glycsylse

EC number: =3.2.2.21

Molecular weight: Translated: 35634; Mature: 35503

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: PS00516 ALKYLBASE_DNA_GLYCOS

Important sites: ACT_SITE 258-258

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWHEVNDVIVITLPEIFDMNANLGYLTREKNECMYEIENNIITKVIAIGEIRSLVQVSV
CCCCCCCCEEEEEEHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
INNKQMIVQFLNDSRPVEQWKREEIVKYIHEWFDLDNDLTPFYEMAKADPLLKMPARKFY
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHCCHHHHC
GLRVIGIPDLFEALCWGVLGQQINLAFAYSLKKQFVEAFGDSIEWNGKKYWVFPPYERIA
CEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEEECCHHHHH
RLTPTDLADIKMTVKKSEYIIGIARLMASGELSREKLMKMNFKDAEKNLIKIRGIGPWTA
CCCCCCHHHHEEEEEHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEEEECCCCCHH
NYVLMRCLRFPTAFPIDDVGLIHSIKILRNMNRKPTKDEILEISVPWKEWQSYATFYLWR
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHEEEEECCHHHHHHHHHHHHHH
VLY
HCC
>Mature Secondary Structure 
TWHEVNDVIVITLPEIFDMNANLGYLTREKNECMYEIENNIITKVIAIGEIRSLVQVSV
CCCCCCCEEEEEEHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
INNKQMIVQFLNDSRPVEQWKREEIVKYIHEWFDLDNDLTPFYEMAKADPLLKMPARKFY
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHCCHHHHC
GLRVIGIPDLFEALCWGVLGQQINLAFAYSLKKQFVEAFGDSIEWNGKKYWVFPPYERIA
CEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEEECCHHHHH
RLTPTDLADIKMTVKKSEYIIGIARLMASGELSREKLMKMNFKDAEKNLIKIRGIGPWTA
CCCCCCHHHHEEEEEHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEEEECCCCCHH
NYVLMRCLRFPTAFPIDDVGLIHSIKILRNMNRKPTKDEILEISVPWKEWQSYATFYLWR
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHEEEEECCHHHHHHHHHHHHHH
VLY
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8376346; 9384377