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The map label for this gene is aroDE

Identifier: 15618943

GI number: 15618943

Start: 1188567

End: 1190000

Strand: Reverse

Name: aroDE

Synonym: CPn1035

Alternate gene names: 15618943

Gene position: 1190000-1188567 (Counterclockwise)

Preceding gene: 15618944

Following gene: 15618942

Centisome position: 96.73

GC content: 43.58

Gene sequence:

>1434_bases
ATGTTATGCGCCACTGTTAGCGGTCCCTCATTTTGTGAAGCAAAACAACAAATTCTAAAGTCTCTACACCTTGTAGATAT
TATAGAACTACGACTCGATCTCATAAACGAACTTGATGATCAAGAACTGCATACCCTCATTACCACAGCCCAAAACCCCA
TCCTCACCTTTAGACAACACAAAGAGATGTCGACAGCGCTATGGATTCAAAAACTATATTCCCTAGCAAAACTTGAACCC
AAGTGGATGGATATCGATGTGAGTCTTCCTAAGACCGCCCTTCAAACCATCCGCAAGAGCCATCCTAAAATAAAGCTCAT
CCTTTCCTATCATACAGACAAAAATGAAGACCTCGATGCAATCTATAATGAGATGCTTGCAACTCCCGCTGAGATCTACA
AAATCGTACTCTCCCCAGAGAACTCCAGTGAAGCTCTGAATTATATAAAAAAAGCTCGCCTGTTACCAAAACCATCTACA
GTCCTTTGTATGGGAACCCATGGGCTCCCCTCTAGAGTTCTCTCCCCACTGATCAGCAACGCTATGAATTACGCTGCTGG
AATCAGTGCGCCTCAAGTAGCCCCGGGACAACCTAAACTTGAGGAGCTGCTCTCCTATAACTACTCGAAGCTATCTGAAA
AATCACACATTTACGGACTCATCGGAGATCCTGTAGACCGTAGCATCAGTCACCTCTCCCACAACTTCTTACTCTCTAAG
CTTTCTCTCAATGCAACATATATCAAATTCCCTGTTACTATAGGGGAAGTCGTCACCTTTTTCTCTGCGATCCGAGATCT
TCCTTTTTCAGGACTTAGTGTCACTATGCCACTAAAAACCGCAATCTTCGACCATGTCGATGCTCTTGATGCATCGGCGC
AACTCTGCGAGTCTATAAACACCCTGGTCTTCCGAAATCAGAAAATTCTAGGTTACAATACAGACGGTGAGGGAGTAGCC
AAACTCCTAAAACAAAAGAATATCTCTGTTAACAACAAACACATTGCCATTGTAGGTGCTGGAGGTGCTGCTAAAGCAAT
CGCTGCAACACTGGCGATGCAAGGTGCTAATCTCCACATCTTCAATAGAACTCTTTCTTCAGCAGCTGCTTTAGCTACTT
GCTGTAAAGGCAAAGCCTATCCCTTAGGCTCCTTAGAGAATTTTAAAACTATAGACATAATCATCAACTGTCTCCCTCCG
GAAGTGACCTTCCCTTGGAGATTCCCTCCTATAGTCATGGACATCAATACCAAACCGCATCCCTCTCCCTACCTTGAACG
TGCTCAAAAACATGGCTCTCTCATTATCCATGGCTACGAAATGTTCATAGAACAAGCCCTGCTACAATTTGCTCTTTGGT
TCCCAGATTTCCTAACTCCAGAGTCTTGTGACTCTTTCCGAAACTATGTAAAAAACTTCATGGCAAAAGTATAA

Upstream 100 bases:

>100_bases
ATCATGATAGAGCATCTAGGTCGTGCAGCTCCCTTTAATGGTACCTATTGTGCATCTCCAAATATGGAGATTCTCTATGA
TATACTATGGAGTGAGTGTC

Downstream 100 bases:

>100_bases
AAAATACTTGTCTTACTCTATCCTTGTCCAAAAGATTGCAAGGTACGTTATGAAAACCTGGTTGTTCTTCACTTTCTTAT
TCTCCTGCTCGTCGTTCTAT

Product: bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase protein

Products: NA

Alternate protein names: 3-dehydroquinate dehydratase; 3-dehydroquinase; Type I DHQase; Shikimate dehydrogenase

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MLCATVSGPSFCEAKQQILKSLHLVDIIELRLDLINELDDQELHTLITTAQNPILTFRQHKEMSTALWIQKLYSLAKLEP
KWMDIDVSLPKTALQTIRKSHPKIKLILSYHTDKNEDLDAIYNEMLATPAEIYKIVLSPENSSEALNYIKKARLLPKPST
VLCMGTHGLPSRVLSPLISNAMNYAAGISAPQVAPGQPKLEELLSYNYSKLSEKSHIYGLIGDPVDRSISHLSHNFLLSK
LSLNATYIKFPVTIGEVVTFFSAIRDLPFSGLSVTMPLKTAIFDHVDALDASAQLCESINTLVFRNQKILGYNTDGEGVA
KLLKQKNISVNNKHIAIVGAGGAAKAIAATLAMQGANLHIFNRTLSSAAALATCCKGKAYPLGSLENFKTIDIIINCLPP
EVTFPWRFPPIVMDINTKPHPSPYLERAQKHGSLIIHGYEMFIEQALLQFALWFPDFLTPESCDSFRNYVKNFMAKV

Sequences:

>Translated_477_residues
MLCATVSGPSFCEAKQQILKSLHLVDIIELRLDLINELDDQELHTLITTAQNPILTFRQHKEMSTALWIQKLYSLAKLEP
KWMDIDVSLPKTALQTIRKSHPKIKLILSYHTDKNEDLDAIYNEMLATPAEIYKIVLSPENSSEALNYIKKARLLPKPST
VLCMGTHGLPSRVLSPLISNAMNYAAGISAPQVAPGQPKLEELLSYNYSKLSEKSHIYGLIGDPVDRSISHLSHNFLLSK
LSLNATYIKFPVTIGEVVTFFSAIRDLPFSGLSVTMPLKTAIFDHVDALDASAQLCESINTLVFRNQKILGYNTDGEGVA
KLLKQKNISVNNKHIAIVGAGGAAKAIAATLAMQGANLHIFNRTLSSAAALATCCKGKAYPLGSLENFKTIDIIINCLPP
EVTFPWRFPPIVMDINTKPHPSPYLERAQKHGSLIIHGYEMFIEQALLQFALWFPDFLTPESCDSFRNYVKNFMAKV
>Mature_477_residues
MLCATVSGPSFCEAKQQILKSLHLVDIIELRLDLINELDDQELHTLITTAQNPILTFRQHKEMSTALWIQKLYSLAKLEP
KWMDIDVSLPKTALQTIRKSHPKIKLILSYHTDKNEDLDAIYNEMLATPAEIYKIVLSPENSSEALNYIKKARLLPKPST
VLCMGTHGLPSRVLSPLISNAMNYAAGISAPQVAPGQPKLEELLSYNYSKLSEKSHIYGLIGDPVDRSISHLSHNFLLSK
LSLNATYIKFPVTIGEVVTFFSAIRDLPFSGLSVTMPLKTAIFDHVDALDASAQLCESINTLVFRNQKILGYNTDGEGVA
KLLKQKNISVNNKHIAIVGAGGAAKAIAATLAMQGANLHIFNRTLSSAAALATCCKGKAYPLGSLENFKTIDIIINCLPP
EVTFPWRFPPIVMDINTKPHPSPYLERAQKHGSLIIHGYEMFIEQALLQFALWFPDFLTPESCDSFRNYVKNFMAKV

Specific function: Bifunctional enzyme that catalyzes two sequential steps of the aromatic amino acids biosynthetic pathway. The first reaction is catalyzed by the 3-dehydroquinate dehydratase, coded by the AroD domain; the second reaction is catalyzed by the shikimate 5-de

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1787983, Length=269, Percent_Identity=29.7397769516729, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789675, Length=253, Percent_Identity=26.4822134387352, Blast_Score=76, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320332, Length=432, Percent_Identity=23.6111111111111, Blast_Score=87, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ARODE_CHLPN (Q9Z6M4)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   G72003
- PIR:   H86619
- RefSeq:   NP_225229.1
- RefSeq:   NP_301090.1
- RefSeq:   NP_445356.1
- RefSeq:   NP_877347.2
- ProteinModelPortal:   Q9Z6M4
- SMR:   Q9Z6M4
- GeneID:   1467754
- GeneID:   894704
- GeneID:   919809
- GeneID:   962771
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0817
- KEGG:   cpn:CPn1035
- KEGG:   cpt:CpB1075
- TIGR:   CP_0817
- HOGENOM:   HBG414053
- OMA:   ISHLSHN
- ProtClustDB:   PRK09310
- BioCyc:   CPNE115711:CP_0817-MONOMER
- BioCyc:   CPNE115713:CPN1035-MONOMER
- BioCyc:   CPNE138677:CPJ1035-MONOMER
- BioCyc:   CPNE182082:CPB1075-MONOMER
- BRENDA:   1.1.1.25
- BRENDA:   4.2.1.10
- GO:   GO:0005737
- HAMAP:   MF_00214
- HAMAP:   MF_00222
- InterPro:   IPR013785
- InterPro:   IPR001381
- InterPro:   IPR016040
- InterPro:   IPR011342
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.20.20.70
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00507

Pfam domain/function: PF01487 DHquinase_I; PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =4.2.1.10; =1.1.1.25

Molecular weight: Translated: 52945; Mature: 52945

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS01028 DEHYDROQUINASE_I

Important sites: ACT_SITE 111-111 ACT_SITE 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLCATVSGPSFCEAKQQILKSLHLVDIIELRLDLINELDDQELHTLITTAQNPILTFRQH
CEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEHHHH
KEMSTALWIQKLYSLAKLEPKWMDIDVSLPKTALQTIRKSHPKIKLILSYHTDKNEDLDA
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCEEEEEEECCCCCCCHHH
IYNEMLATPAEIYKIVLSPENSSEALNYIKKARLLPKPSTVLCMGTHGLPSRVLSPLISN
HHHHHHCCHHHHEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHH
AMNYAAGISAPQVAPGQPKLEELLSYNYSKLSEKSHIYGLIGDPVDRSISHLSHNFLLSK
HHHHHHCCCCCCCCCCCHHHHHHHCCCHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHH
LSLNATYIKFPVTIGEVVTFFSAIRDLPFSGLSVTMPLKTAIFDHVDALDASAQLCESIN
HCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHH
TLVFRNQKILGYNTDGEGVAKLLKQKNISVNNKHIAIVGAGGAAKAIAATLAMQGANLHI
HHHEECCEEEEECCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEE
FNRTLSSAAALATCCKGKAYPLGSLENFKTIDIIINCLPPEVTFPWRFPPIVMDINTKPH
EHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEECCCCCC
PSPYLERAQKHGSLIIHGYEMFIEQALLQFALWFPDFLTPESCDSFRNYVKNFMAKV
CCHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLCATVSGPSFCEAKQQILKSLHLVDIIELRLDLINELDDQELHTLITTAQNPILTFRQH
CEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEHHHH
KEMSTALWIQKLYSLAKLEPKWMDIDVSLPKTALQTIRKSHPKIKLILSYHTDKNEDLDA
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCEEEEEEECCCCCCCHHH
IYNEMLATPAEIYKIVLSPENSSEALNYIKKARLLPKPSTVLCMGTHGLPSRVLSPLISN
HHHHHHCCHHHHEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHH
AMNYAAGISAPQVAPGQPKLEELLSYNYSKLSEKSHIYGLIGDPVDRSISHLSHNFLLSK
HHHHHHCCCCCCCCCCCHHHHHHHCCCHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHH
LSLNATYIKFPVTIGEVVTFFSAIRDLPFSGLSVTMPLKTAIFDHVDALDASAQLCESIN
HCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHH
TLVFRNQKILGYNTDGEGVAKLLKQKNISVNNKHIAIVGAGGAAKAIAATLAMQGANLHI
HHHEECCEEEEECCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEE
FNRTLSSAAALATCCKGKAYPLGSLENFKTIDIIINCLPPEVTFPWRFPPIVMDINTKPH
EHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEECCCCCC
PSPYLERAQKHGSLIIHGYEMFIEQALLQFALWFPDFLTPESCDSFRNYVKNFMAKV
CCHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362