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The map label for this gene is cysS

Identifier: 15618841

GI number: 15618841

Start: 1065718

End: 1067142

Strand: Reverse

Name: cysS

Synonym: CPn0932

Alternate gene names: 15618841

Gene position: 1067142-1065718 (Counterclockwise)

Preceding gene: 15618843

Following gene: 15618838

Centisome position: 86.74

GC content: 42.25

Gene sequence:

>1425_bases
ATGGCATTTTCTCATATCGAGGGTCTCTATTTCTACAATACAGCATCTCAAAAAAAAGAACTTTTTTTCCCCAATCACAC
CCCTGTAAGACTATATACTTGTGGTCCTACCGTGTATGACTACGCTCACATAGGGAACTTCCGAACTTATGTCTTTGAAG
ATATTCTCAAACGCACTCTAGTATTCTTTGGCTACTCTGTAACCCATGTGATGAATATTACTGATGTAGAAGACAAAACC
ATAGCAGGAGCCTCGAAAAAAAATATTCCGCTGCAAGAATACACGCAGCCATATACCGAAGCCTTTTTTGAAGATCTCGA
TACTTTAAATATTGCTAGAGCAGATTTCTATCCACACGCAACACACTACATCCCTCAAATGATCCAAGCGATTACAAAGC
TCTTAGAGCAAGGAATCGCCTATATAGGTCAGGATGCTTCCGTATATTTTTCTCTAAACCGTTTTCCAAATTATGGGAAG
CTCTCTCATCTGGATCTAAGTAGCTTACGCTGCTGCTCCAGAATTTCTGCTGATGAGTATGACAAAGAAAACCCAAGTGA
TTTTGTACTGTGGAAAGCATACAATCCAGAACGTGATGGCGTCATTTATTGGGAAAGTCCTTTCGGGAAAGGAAGACCTG
GATGGCATTTAGAATGTTCGATTATGGCGATGGAACTTCTTGGAGATTCTTTGGATATCCATGCGGGAGGTGTAGATAAT
ATATTTCCTCATCACGAAAATGAAATCGCCCAATCTGAGGCTCTTTCAGGGAAACCCTTTGCACGCTATTGGCTGCATTC
GGAACATCTGCTTATTGATGGGAAGAAAATGTCAAAGAGCTTAGGGAATTTCCTCACTCTACGAGACCTTCTCCACCAGG
AATTTACTGGTCAAGAAGTACGCTATATGCTACTGCAAAGCCATTATCGTACGCAACTCAACTTTACAGAAGAAGCTCTC
CTTGCCTGTAGGCATGCCTTACGCCGCTTAAAAGATTTTGTTTCTCGACTTGAAGGCGTAGATCTCCCTGGGGAATCTCC
CCTTCCCAGAACCTTGGACTCTAGCAGTCAATTCATAGAAGCTTTCTCAAGAGCTCTTGCTAATGACTTAAATGTTTCTA
CAGGATTTGCATCTCTGTTCGATTTTGTTCACGAAATCAACACCTTGATCGACCAAGGGCACTTTTCTAAAGCCGACTCT
CTCTATATCTTAGACACTCTTAAAAAAGTCGATACTGTACTTGGTGTGCTTCCTCTAACCACAAGCGTGTGCATCCCAGA
AACCGTAATGCAACTTGTTGCAGAACGTGAGGAAGCTAGAAAAACTAAAAATTGGGCGATGGCAGACACCTTACGTGATG
AGATACTTGCCGCAGGCTTCCTTGTCGAAGACTCTAAGTCAGGGCCTAAAGTTAAGCCCCTTTAA

Upstream 100 bases:

>100_bases
GATCTAGAAAATTTATTTTGTTAGGATAGGCCGAAGAGACTGACGTTAAATTCTCTCTGTCTATATAACAACAAACTCCT
GAGTCAAGAGTGATACAGTT

Downstream 100 bases:

>100_bases
TTCGTCTTCTCTGCATCGAAGCGCCGCATTACAGGGAAATACAGCACATCGCGTATAGATGCAGCATTTGTAAGAATCAT
AACAAGTCTATCGACACCTA

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MAFSHIEGLYFYNTASQKKELFFPNHTPVRLYTCGPTVYDYAHIGNFRTYVFEDILKRTLVFFGYSVTHVMNITDVEDKT
IAGASKKNIPLQEYTQPYTEAFFEDLDTLNIARADFYPHATHYIPQMIQAITKLLEQGIAYIGQDASVYFSLNRFPNYGK
LSHLDLSSLRCCSRISADEYDKENPSDFVLWKAYNPERDGVIYWESPFGKGRPGWHLECSIMAMELLGDSLDIHAGGVDN
IFPHHENEIAQSEALSGKPFARYWLHSEHLLIDGKKMSKSLGNFLTLRDLLHQEFTGQEVRYMLLQSHYRTQLNFTEEAL
LACRHALRRLKDFVSRLEGVDLPGESPLPRTLDSSSQFIEAFSRALANDLNVSTGFASLFDFVHEINTLIDQGHFSKADS
LYILDTLKKVDTVLGVLPLTTSVCIPETVMQLVAEREEARKTKNWAMADTLRDEILAAGFLVEDSKSGPKVKPL

Sequences:

>Translated_474_residues
MAFSHIEGLYFYNTASQKKELFFPNHTPVRLYTCGPTVYDYAHIGNFRTYVFEDILKRTLVFFGYSVTHVMNITDVEDKT
IAGASKKNIPLQEYTQPYTEAFFEDLDTLNIARADFYPHATHYIPQMIQAITKLLEQGIAYIGQDASVYFSLNRFPNYGK
LSHLDLSSLRCCSRISADEYDKENPSDFVLWKAYNPERDGVIYWESPFGKGRPGWHLECSIMAMELLGDSLDIHAGGVDN
IFPHHENEIAQSEALSGKPFARYWLHSEHLLIDGKKMSKSLGNFLTLRDLLHQEFTGQEVRYMLLQSHYRTQLNFTEEAL
LACRHALRRLKDFVSRLEGVDLPGESPLPRTLDSSSQFIEAFSRALANDLNVSTGFASLFDFVHEINTLIDQGHFSKADS
LYILDTLKKVDTVLGVLPLTTSVCIPETVMQLVAEREEARKTKNWAMADTLRDEILAAGFLVEDSKSGPKVKPL
>Mature_473_residues
AFSHIEGLYFYNTASQKKELFFPNHTPVRLYTCGPTVYDYAHIGNFRTYVFEDILKRTLVFFGYSVTHVMNITDVEDKTI
AGASKKNIPLQEYTQPYTEAFFEDLDTLNIARADFYPHATHYIPQMIQAITKLLEQGIAYIGQDASVYFSLNRFPNYGKL
SHLDLSSLRCCSRISADEYDKENPSDFVLWKAYNPERDGVIYWESPFGKGRPGWHLECSIMAMELLGDSLDIHAGGVDNI
FPHHENEIAQSEALSGKPFARYWLHSEHLLIDGKKMSKSLGNFLTLRDLLHQEFTGQEVRYMLLQSHYRTQLNFTEEALL
ACRHALRRLKDFVSRLEGVDLPGESPLPRTLDSSSQFIEAFSRALANDLNVSTGFASLFDFVHEINTLIDQGHFSKADSL
YILDTLKKVDTVLGVLPLTTSVCIPETVMQLVAEREEARKTKNWAMADTLRDEILAAGFLVEDSKSGPKVKPL

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=393, Percent_Identity=37.4045801526718, Blast_Score=235, Evalue=7e-62,
Organism=Homo sapiens, GI10835051, Length=453, Percent_Identity=30.6843267108168, Blast_Score=176, Evalue=5e-44,
Organism=Homo sapiens, GI21269877, Length=453, Percent_Identity=30.6843267108168, Blast_Score=176, Evalue=5e-44,
Organism=Homo sapiens, GI62240992, Length=453, Percent_Identity=30.6843267108168, Blast_Score=176, Evalue=6e-44,
Organism=Homo sapiens, GI303304987, Length=453, Percent_Identity=30.6843267108168, Blast_Score=175, Evalue=6e-44,
Organism=Escherichia coli, GI1786737, Length=432, Percent_Identity=39.5833333333333, Blast_Score=306, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI17509795, Length=244, Percent_Identity=36.4754098360656, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17509797, Length=244, Percent_Identity=36.4754098360656, Blast_Score=162, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6324082, Length=316, Percent_Identity=33.8607594936709, Blast_Score=181, Evalue=3e-46,
Organism=Drosophila melanogaster, GI19922218, Length=416, Percent_Identity=36.5384615384615, Blast_Score=245, Evalue=5e-65,
Organism=Drosophila melanogaster, GI19922364, Length=356, Percent_Identity=32.0224719101124, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_CHLPN (Q9Z6X4)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   B86607
- PIR:   F72016
- RefSeq:   NP_225127.1
- RefSeq:   NP_300989.1
- RefSeq:   NP_445468.1
- RefSeq:   NP_877238.1
- ProteinModelPortal:   Q9Z6X4
- SMR:   Q9Z6X4
- PHCI-2DPAGE:   Q9Z6X4
- GeneID:   1467645
- GeneID:   895249
- GeneID:   919694
- GeneID:   963392
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0931
- KEGG:   cpn:CPn0932
- KEGG:   cpt:CpB0966
- TIGR:   CP_0931
- HOGENOM:   HBG327651
- OMA:   IGNLRTY
- ProtClustDB:   PRK00260
- BioCyc:   CPNE115711:CP_0931-MONOMER
- BioCyc:   CPNE115713:CPN0932-MONOMER
- BioCyc:   CPNE138677:CPJ0932-MONOMER
- BioCyc:   CPNE182082:CPB0966-MONOMER
- BRENDA:   6.1.1.16
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 53930; Mature: 53799

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 279-279

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFSHIEGLYFYNTASQKKELFFPNHTPVRLYTCGPTVYDYAHIGNFRTYVFEDILKRTL
CCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHH
VFFGYSVTHVMNITDVEDKTIAGASKKNIPLQEYTQPYTEAFFEDLDTLNIARADFYPHA
HHHCCHHHHEEECCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCCH
THYIPQMIQAITKLLEQGIAYIGQDASVYFSLNRFPNYGKLSHLDLSSLRCCSRISADEY
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCC
DKENPSDFVLWKAYNPERDGVIYWESPFGKGRPGWHLECSIMAMELLGDSLDIHAGGVDN
CCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCEEEHHHHHHHHHCCCCEEECCCCCC
IFPHHENEIAQSEALSGKPFARYWLHSEHLLIDGKKMSKSLGNFLTLRDLLHQEFTGQEV
CCCCCCHHHHHHHHCCCCCHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCHHH
RYMLLQSHYRTQLNFTEEALLACRHALRRLKDFVSRLEGVDLPGESPLPRTLDSSSQFIE
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
AFSRALANDLNVSTGFASLFDFVHEINTLIDQGHFSKADSLYILDTLKKVDTVLGVLPLT
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TSVCIPETVMQLVAEREEARKTKNWAMADTLRDEILAAGFLVEDSKSGPKVKPL
HHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEECCCCCCCCCCC
>Mature Secondary Structure 
AFSHIEGLYFYNTASQKKELFFPNHTPVRLYTCGPTVYDYAHIGNFRTYVFEDILKRTL
CCCCCCCEEEECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHH
VFFGYSVTHVMNITDVEDKTIAGASKKNIPLQEYTQPYTEAFFEDLDTLNIARADFYPHA
HHHCCHHHHEEECCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCCH
THYIPQMIQAITKLLEQGIAYIGQDASVYFSLNRFPNYGKLSHLDLSSLRCCSRISADEY
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCC
DKENPSDFVLWKAYNPERDGVIYWESPFGKGRPGWHLECSIMAMELLGDSLDIHAGGVDN
CCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCEEEHHHHHHHHHCCCCEEECCCCCC
IFPHHENEIAQSEALSGKPFARYWLHSEHLLIDGKKMSKSLGNFLTLRDLLHQEFTGQEV
CCCCCCHHHHHHHHCCCCCHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCHHH
RYMLLQSHYRTQLNFTEEALLACRHALRRLKDFVSRLEGVDLPGESPLPRTLDSSSQFIE
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
AFSRALANDLNVSTGFASLFDFVHEINTLIDQGHFSKADSLYILDTLKKVDTVLGVLPLT
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TSVCIPETVMQLVAEREEARKTKNWAMADTLRDEILAAGFLVEDSKSGPKVKPL
HHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362