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Identifier: 15618820

GI number: 15618820

Start: 1042982

End: 1044043

Strand: Reverse

Name: Not Available

Synonym: CPn0911

Alternate gene names: 15618820

Gene position: 1044043-1042982 (Counterclockwise)

Preceding gene: 15618827

Following gene: 15618817

Centisome position: 84.87

GC content: 42.0

Gene sequence:

>1062_bases
ATGAATTTATGTAAAAGAATTTCATTTGAAGAAGGATTAGAGCTATTTGTTTCGTCCCCTATCGAGAGATTACAAGAGCG
TGCGGACGCGATTCGTAAAGAACGATATCCTTCAAATGAAGTTACTTATGTTTTGGATGCCAATCCAAACTATACTAATA
TTTGTAAAATAGATTGTACGTTTTGTGCGTTCTACAGAAAGCCTAAATCTCCCGACGCTTATCTATTATCTTTTGATGAA
GTTCGCAGCCTATTACAACGTTATGTAAGTTCAGGAGTCAAAACTGTTTTACTGCAAGGTGGTGTGCACCCGGGGTTAGG
CATCGATTATCTTGAAGAACTCGTACGCATTACTGTTCAAGAATTTCCTTCCATCCATCCTCATTTCTTTTCTGCAGTAG
AAATCGAACATGCTTGCCGGGTCTCTGGCATCAGTATTGAGCAAGGTCTCCAAAGGTTATGGGATGCGGGGCAACGTACC
ATCCCTGGGGGCGGGGCTGAGATTCTTTCAGAGAGAGTTAGAAAAATCATCTCTCCAAAAAAAATGCAGCCTGGGGGCTG
GATCAATCTACATAAGTTAGCCCATCTCATGGGCTTTCGCACAACAGCAACAATGATGTTTGGGCATGTGGAGAACCCAG
AAGATATCCTTATACATCTTCAAACACTCCGTGATGCTCAAGATAGCTGCCCTGGCTTTTATAGTTTTATCCCCTGGAGT
TATAAACCTGGGAATACGGCCCTAAGACGTAACGTTCCTCAACAAGCTTCTATTGAAACCTATTACCGCATTTTAGCTTT
GGGCAGAATCTTTCTGGATAATTTTGATCACGTAGCAGCCTCATGGTTTGGCGAGGGTAAAAGCTTAGGAGCGAAAGCTC
TCCATTACGGAGCTGATGATTTTGGAGGGGTTATTTTAGACGAAAGCGTGCATAAAGCTACAGGATGGTCGATACAGAGC
TCTGAAGAAGAAATCTGTAATATCATTCGATCTGAAGGTTTCATTCCTGTGGAGAGAAATACCTTTTATCAACACATCAG
CTGTACAGTAAGTAGTCTTTAG

Upstream 100 bases:

>100_bases
GTCTGAATTATCCATAATAACAACTTAGGTGTGGGGCTTGACAAATAAAATTGTTAAAACTTAAAGTCTGCTGCTAGCAA
TCTTTAACGTCAATTATTTT

Downstream 100 bases:

>100_bases
CTATCTTCTGAGCAATTGCGTCCGAAGAGAGGCCTAATGTCGGAAGTTCTCGAAATATCGAATAACGTTTAAACCATGTT
TTTTGTTTTTTAGTATAATG

Product: hypothetical protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MNLCKRISFEEGLELFVSSPIERLQERADAIRKERYPSNEVTYVLDANPNYTNICKIDCTFCAFYRKPKSPDAYLLSFDE
VRSLLQRYVSSGVKTVLLQGGVHPGLGIDYLEELVRITVQEFPSIHPHFFSAVEIEHACRVSGISIEQGLQRLWDAGQRT
IPGGGAEILSERVRKIISPKKMQPGGWINLHKLAHLMGFRTTATMMFGHVENPEDILIHLQTLRDAQDSCPGFYSFIPWS
YKPGNTALRRNVPQQASIETYYRILALGRIFLDNFDHVAASWFGEGKSLGAKALHYGADDFGGVILDESVHKATGWSIQS
SEEEICNIIRSEGFIPVERNTFYQHISCTVSSL

Sequences:

>Translated_353_residues
MNLCKRISFEEGLELFVSSPIERLQERADAIRKERYPSNEVTYVLDANPNYTNICKIDCTFCAFYRKPKSPDAYLLSFDE
VRSLLQRYVSSGVKTVLLQGGVHPGLGIDYLEELVRITVQEFPSIHPHFFSAVEIEHACRVSGISIEQGLQRLWDAGQRT
IPGGGAEILSERVRKIISPKKMQPGGWINLHKLAHLMGFRTTATMMFGHVENPEDILIHLQTLRDAQDSCPGFYSFIPWS
YKPGNTALRRNVPQQASIETYYRILALGRIFLDNFDHVAASWFGEGKSLGAKALHYGADDFGGVILDESVHKATGWSIQS
SEEEICNIIRSEGFIPVERNTFYQHISCTVSSL
>Mature_353_residues
MNLCKRISFEEGLELFVSSPIERLQERADAIRKERYPSNEVTYVLDANPNYTNICKIDCTFCAFYRKPKSPDAYLLSFDE
VRSLLQRYVSSGVKTVLLQGGVHPGLGIDYLEELVRITVQEFPSIHPHFFSAVEIEHACRVSGISIEQGLQRLWDAGQRT
IPGGGAEILSERVRKIISPKKMQPGGWINLHKLAHLMGFRTTATMMFGHVENPEDILIHLQTLRDAQDSCPGFYSFIPWS
YKPGNTALRRNVPQQASIETYYRILALGRIFLDNFDHVAASWFGEGKSLGAKALHYGADDFGGVILDESVHKATGWSIQS
SEEEICNIIRSEGFIPVERNTFYQHISCTVSSL

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 39822; Mature: 39822

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLCKRISFEEGLELFVSSPIERLQERADAIRKERYPSNEVTYVLDANPNYTNICKIDCT
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEHH
FCAFYRKPKSPDAYLLSFDEVRSLLQRYVSSGVKTVLLQGGVHPGLGIDYLEELVRITVQ
HHHHHCCCCCCCCEEEEHHHHHHHHHHHHHCCHHEEHHCCCCCCCCCHHHHHHHHHHHHH
EFPSIHPHFFSAVEIEHACRVSGISIEQGLQRLWDAGQRTIPGGGAEILSERVRKIISPK
HCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCC
KMQPGGWINLHKLAHLMGFRTTATMMFGHVENPEDILIHLQTLRDAQDSCPGFYSFIPWS
CCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCCCC
YKPGNTALRRNVPQQASIETYYRILALGRIFLDNFDHVAASWFGEGKSLGAKALHYGADD
CCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHCCHHHHHCCCCC
FGGVILDESVHKATGWSIQSSEEEICNIIRSEGFIPVERNTFYQHISCTVSSL
CCCEEECCCHHHHCCCCCCCCHHHHHHHHHHCCCEECCCCHHHHHHHEEECCC
>Mature Secondary Structure
MNLCKRISFEEGLELFVSSPIERLQERADAIRKERYPSNEVTYVLDANPNYTNICKIDCT
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEHH
FCAFYRKPKSPDAYLLSFDEVRSLLQRYVSSGVKTVLLQGGVHPGLGIDYLEELVRITVQ
HHHHHCCCCCCCCEEEEHHHHHHHHHHHHHCCHHEEHHCCCCCCCCCHHHHHHHHHHHHH
EFPSIHPHFFSAVEIEHACRVSGISIEQGLQRLWDAGQRTIPGGGAEILSERVRKIISPK
HCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCC
KMQPGGWINLHKLAHLMGFRTTATMMFGHVENPEDILIHLQTLRDAQDSCPGFYSFIPWS
CCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCCCC
YKPGNTALRRNVPQQASIETYYRILALGRIFLDNFDHVAASWFGEGKSLGAKALHYGADD
CCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHCCHHHHHCCCCC
FGGVILDESVHKATGWSIQSSEEEICNIIRSEGFIPVERNTFYQHISCTVSSL
CCCEEECCCHHHHCCCCCCCCHHHHHHHHHHCCCEECCCCHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA