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The map label for this gene is birA

Identifier: 15618775

GI number: 15618775

Start: 982913

End: 983491

Strand: Reverse

Name: birA

Synonym: CPn0866

Alternate gene names: 15618775

Gene position: 983491-982913 (Counterclockwise)

Preceding gene: 15618777

Following gene: 15618772

Centisome position: 79.94

GC content: 41.62

Gene sequence:

>579_bases
ATGAAAGTTATTTATTACGAAATAGAAGAAATTCCCTCCACGAACACAATGGCAAAATCCTATATGCATTTGTGGGATCC
TTATGCATTAACTGTAATCTCCACAAAATGTCAAACTGCGGGCACAGGAAAATTTGGTAAAAGCTGGAAATCTTCAAAGG
GGGATCTTCTTAATACCTTCTGTTTTTTTATTACAGACCTCCACATCGATGTATCACGTCTCTTTCGCTTAGGCACAGAA
GCTGTTGTTGCCTTATGTAAGGATTTAGGAATTACAGAAGCAAAAATAAAATGGCCTAACGATGTCCTGGTTCATGGAGA
AAAGCTCTGTGGAGTTCTTCCAGAGACTCTCCCTGTAGAAGGACTTCTTGGAGTCGTTCTTGGGATCGGCCTCAATGGCA
ATACTACGAAACAAGCTTTAAAAGATGTCGGACAACCTGCAACATCCCTTCAAGAGATTCTTGGCCATCCCATAGACCTT
GAGACTACCAGGGAGCTTCTTATACACCATCTCCTAGGAGTGTTGCAGGAGAATCTTCCCGATAGCTTAGCTACTAAATC
GAATCGAGGGAACATCTAA

Upstream 100 bases:

>100_bases
AGGTAAGTACGACAAGAAAAACCCAAGAATTCACATACCGAAAATATTTATGATATCTCATAATCGTATTACTATTGAGC
AGACTGTAAATTTCTAGAAT

Downstream 100 bases:

>100_bases
AAAGCGGAACAACTTTGAGGCAAGTCTCAAAGGGGCTCGCTGTCGCAACTTAAGATACTTCTCCACAGCTTCTTCGTAAG
CTGCAATCTCAAAATCAAAT

Product: biotin--protein ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MKVIYYEIEEIPSTNTMAKSYMHLWDPYALTVISTKCQTAGTGKFGKSWKSSKGDLLNTFCFFITDLHIDVSRLFRLGTE
AVVALCKDLGITEAKIKWPNDVLVHGEKLCGVLPETLPVEGLLGVVLGIGLNGNTTKQALKDVGQPATSLQEILGHPIDL
ETTRELLIHHLLGVLQENLPDSLATKSNRGNI

Sequences:

>Translated_192_residues
MKVIYYEIEEIPSTNTMAKSYMHLWDPYALTVISTKCQTAGTGKFGKSWKSSKGDLLNTFCFFITDLHIDVSRLFRLGTE
AVVALCKDLGITEAKIKWPNDVLVHGEKLCGVLPETLPVEGLLGVVLGIGLNGNTTKQALKDVGQPATSLQEILGHPIDL
ETTRELLIHHLLGVLQENLPDSLATKSNRGNI
>Mature_192_residues
MKVIYYEIEEIPSTNTMAKSYMHLWDPYALTVISTKCQTAGTGKFGKSWKSSKGDLLNTFCFFITDLHIDVSRLFRLGTE
AVVALCKDLGITEAKIKWPNDVLVHGEKLCGVLPETLPVEGLLGVVLGIGLNGNTTKQALKDVGQPATSLQEILGHPIDL
ETTRELLIHHLLGVLQENLPDSLATKSNRGNI

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 21057; Mature: 21057

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIYYEIEEIPSTNTMAKSYMHLWDPYALTVISTKCQTAGTGKFGKSWKSSKGDLLNTF
CEEEEEEHHHCCCCHHHHHHHHHHCCCHHEEEEHHHCCCCCCCCCCCCCCCCCCHHHHHH
CFFITDLHIDVSRLFRLGTEAVVALCKDLGITEAKIKWPNDVLVHGEKLCGVLPETLPVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEECCHHHHHCCCCCCCHH
GLLGVVLGIGLNGNTTKQALKDVGQPATSLQEILGHPIDLETTRELLIHHLLGVLQENLP
HHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
DSLATKSNRGNI
HHHHCCCCCCCC
>Mature Secondary Structure
MKVIYYEIEEIPSTNTMAKSYMHLWDPYALTVISTKCQTAGTGKFGKSWKSSKGDLLNTF
CEEEEEEHHHCCCCHHHHHHHHHHCCCHHEEEEHHHCCCCCCCCCCCCCCCCCCHHHHHH
CFFITDLHIDVSRLFRLGTEAVVALCKDLGITEAKIKWPNDVLVHGEKLCGVLPETLPVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEECCHHHHHCCCCCCCHH
GLLGVVLGIGLNGNTTKQALKDVGQPATSLQEILGHPIDLETTRELLIHHLLGVLQENLP
HHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
DSLATKSNRGNI
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]