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The map label for this gene is tig

Identifier: 15618757

GI number: 15618757

Start: 960174

End: 961502

Strand: Reverse

Name: tig

Synonym: CPn0848

Alternate gene names: 15618757

Gene position: 961502-960174 (Counterclockwise)

Preceding gene: 15618763

Following gene: 15618756

Centisome position: 78.16

GC content: 39.2

Gene sequence:

>1329_bases
GTGCCACGTAGTCTCTCCAATGAGCAGTTCTCCGTCGATTTAGAAGAATCTCCTGGTTGTATTGTCTCAGCTTTAGTAAA
AGTCTCCCCAGAAGTACTTAACAAACTCAACAAGCAAGCTCTTAAAAAGATTAAAAAAGAAATTACACTTCCAGGATTCC
GCAAAGGAAAAGCCCCTGATGATGTAATTGCATCCCGATATCCTACTAACGTAAGAAAAGAGTTAGGAGAACTAGTAACT
CAAGACGCTTATCATGCCTTATCTACAGTCGGAGATCGTCGTCCTCTTTCGCCTAAAGCTGTCCGTTCGAACTCAATCAC
TCAATTCGATCTTCAAGAAGGAGCCAAAGTAGAGTTCTCTTATGAAGCTTTCCCTGCAATTTCTGATCTTCCTTGGGAAA
ACCTTTCTTTACCTCAGGAAGAAGCTGCTAGTGAGATTTCAGATAGTGATATCGAGAAGGGACTCACAAACATTGGTATG
TTCTTTGCAACAAAAACTCCTGTAGAACGTCCTTCCCAAGAAGGCGATTTCATTTCTATTTCCTTGCACGTCTCTAAAAG
TAATGATGAGAATGCTTCCTCTGCAGCGATTTTTGAAAACAAATATTTTAAGCTTTCTGAAGAAGAAATGACCGATGCCT
TTAAAGAAAAATTCTTAGGAATTTCTACTGGACATCGTGTTGTTGAGACAATTACCTCCCCTGAAATTCAATCCTTCTTA
AGAGGAGATACTTTAACATTTACAGTAAACGCTGTAATCGAAGTCTCGATCCCCGAGATAGATGACGAAAAAGCTCGTCA
ACTACAAGCAGAATCTCTAGACGATTTAAAAGCGAAATTACGCATACAACTAGAGAAGCAAGCTAAAGATAAACAATTGC
AAAAACGCTTTTCTGAAGCTGAAGATGCTCTTGCCATGCTCGTAGATTTTGAACTTCCTACCTCCCTGCTTGAAGAACGT
ATTTCCTTAATCACAAGAGAAAAACTTCTTAATGCACGTCTTATCCAATATTGTTCTGATGAAGAACTTGAAAAAAGAAA
ATCAGAACTTATCAAGGAAGCAGAAGAAGATGCTACAAAAGCATTAAAGTTACTGTTTTTGACTCATAAAATTTTCTCTG
ATGAAAAACTCACCATTAGCCGCGAAGAACTGCAATACATGATGGATGTATGTTCAAGAGAGCGCTTCGGACAACAACCT
CCTAAAGATATTTCCAATGACACCTTACAAGAACTTGTAATGTCAGCCCGCGATCGGCTGACCTATAGCAAAGCTATCGA
ACATGTTCTTCGCAAAGCAGAACTGTTGGCTTCAACTCCTTCTGCATAA

Upstream 100 bases:

>100_bases
TGTAGCTCAGTGGTAGAGCGTCACGTTGCCAACGTGAAGGTCGTGAGTTCAAGCCTCATCACCCGCTTTTCCTTTCAAGT
CTAATAAAAAGGGTTGTCTT

Downstream 100 bases:

>100_bases
ATTTTAGTTAGAGGAAAATACTTGACCCAAGAAAGTCTTAAACATAGAATTCGAGATTTTAATGTCTAAGCTTGTTGCTG
ACGTAGACAGCCTAAAAAGT

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MPRSLSNEQFSVDLEESPGCIVSALVKVSPEVLNKLNKQALKKIKKEITLPGFRKGKAPDDVIASRYPTNVRKELGELVT
QDAYHALSTVGDRRPLSPKAVRSNSITQFDLQEGAKVEFSYEAFPAISDLPWENLSLPQEEAASEISDSDIEKGLTNIGM
FFATKTPVERPSQEGDFISISLHVSKSNDENASSAAIFENKYFKLSEEEMTDAFKEKFLGISTGHRVVETITSPEIQSFL
RGDTLTFTVNAVIEVSIPEIDDEKARQLQAESLDDLKAKLRIQLEKQAKDKQLQKRFSEAEDALAMLVDFELPTSLLEER
ISLITREKLLNARLIQYCSDEELEKRKSELIKEAEEDATKALKLLFLTHKIFSDEKLTISREELQYMMDVCSRERFGQQP
PKDISNDTLQELVMSARDRLTYSKAIEHVLRKAELLASTPSA

Sequences:

>Translated_442_residues
MPRSLSNEQFSVDLEESPGCIVSALVKVSPEVLNKLNKQALKKIKKEITLPGFRKGKAPDDVIASRYPTNVRKELGELVT
QDAYHALSTVGDRRPLSPKAVRSNSITQFDLQEGAKVEFSYEAFPAISDLPWENLSLPQEEAASEISDSDIEKGLTNIGM
FFATKTPVERPSQEGDFISISLHVSKSNDENASSAAIFENKYFKLSEEEMTDAFKEKFLGISTGHRVVETITSPEIQSFL
RGDTLTFTVNAVIEVSIPEIDDEKARQLQAESLDDLKAKLRIQLEKQAKDKQLQKRFSEAEDALAMLVDFELPTSLLEER
ISLITREKLLNARLIQYCSDEELEKRKSELIKEAEEDATKALKLLFLTHKIFSDEKLTISREELQYMMDVCSRERFGQQP
PKDISNDTLQELVMSARDRLTYSKAIEHVLRKAELLASTPSA
>Mature_441_residues
PRSLSNEQFSVDLEESPGCIVSALVKVSPEVLNKLNKQALKKIKKEITLPGFRKGKAPDDVIASRYPTNVRKELGELVTQ
DAYHALSTVGDRRPLSPKAVRSNSITQFDLQEGAKVEFSYEAFPAISDLPWENLSLPQEEAASEISDSDIEKGLTNIGMF
FATKTPVERPSQEGDFISISLHVSKSNDENASSAAIFENKYFKLSEEEMTDAFKEKFLGISTGHRVVETITSPEIQSFLR
GDTLTFTVNAVIEVSIPEIDDEKARQLQAESLDDLKAKLRIQLEKQAKDKQLQKRFSEAEDALAMLVDFELPTSLLEERI
SLITREKLLNARLIQYCSDEELEKRKSELIKEAEEDATKALKLLFLTHKIFSDEKLTISREELQYMMDVCSRERFGQQPP
KDISNDTLQELVMSARDRLTYSKAIEHVLRKAELLASTPSA

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=437, Percent_Identity=21.5102974828375, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_CHLPN (Q9Z758)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   F86596
- PIR:   H72028
- RefSeq:   NP_225043.1
- RefSeq:   NP_300905.1
- RefSeq:   NP_445558.1
- RefSeq:   NP_877149.1
- ProteinModelPortal:   Q9Z758
- PHCI-2DPAGE:   Q9Z758
- GeneID:   1467556
- GeneID:   894678
- GeneID:   919610
- GeneID:   963498
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP1021
- KEGG:   cpn:CPn0848
- KEGG:   cpt:CpB0877
- TIGR:   CP_1021
- HOGENOM:   HBG402733
- OMA:   NARLIQY
- ProtClustDB:   PRK01490
- BioCyc:   CPNE115711:CP_1021-MONOMER
- BioCyc:   CPNE115713:CPN0848-MONOMER
- BioCyc:   CPNE138677:CPJ0848-MONOMER
- BioCyc:   CPNE182082:CPB0877-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 49840; Mature: 49709

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRSLSNEQFSVDLEESPGCIVSALVKVSPEVLNKLNKQALKKIKKEITLPGFRKGKAPD
CCCCCCCCCEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
DVIASRYPTNVRKELGELVTQDAYHALSTVGDRRPLSPKAVRSNSITQFDLQEGAKVEFS
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCEEEECCCCCEEEEE
YEAFPAISDLPWENLSLPQEEAASEISDSDIEKGLTNIGMFFATKTPVERPSQEGDFISI
HHHCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCEEEE
SLHVSKSNDENASSAAIFENKYFKLSEEEMTDAFKEKFLGISTGHRVVETITSPEIQSFL
EEEEECCCCCCCCHHHHECCCCEECCHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHH
RGDTLTFTVNAVIEVSIPEIDDEKARQLQAESLDDLKAKLRIQLEKQAKDKQLQKRFSEA
CCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDALAMLVDFELPTSLLEERISLITREKLLNARLIQYCSDEELEKRKSELIKEAEEDATK
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
ALKLLFLTHKIFSDEKLTISREELQYMMDVCSRERFGQQPPKDISNDTLQELVMSARDRL
HHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
TYSKAIEHVLRKAELLASTPSA
HHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PRSLSNEQFSVDLEESPGCIVSALVKVSPEVLNKLNKQALKKIKKEITLPGFRKGKAPD
CCCCCCCCEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
DVIASRYPTNVRKELGELVTQDAYHALSTVGDRRPLSPKAVRSNSITQFDLQEGAKVEFS
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCEEEECCCCCEEEEE
YEAFPAISDLPWENLSLPQEEAASEISDSDIEKGLTNIGMFFATKTPVERPSQEGDFISI
HHHCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCEEEE
SLHVSKSNDENASSAAIFENKYFKLSEEEMTDAFKEKFLGISTGHRVVETITSPEIQSFL
EEEEECCCCCCCCHHHHECCCCEECCHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHH
RGDTLTFTVNAVIEVSIPEIDDEKARQLQAESLDDLKAKLRIQLEKQAKDKQLQKRFSEA
CCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDALAMLVDFELPTSLLEERISLITREKLLNARLIQYCSDEELEKRKSELIKEAEEDATK
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
ALKLLFLTHKIFSDEKLTISREELQYMMDVCSRERFGQQPPKDISNDTLQELVMSARDRL
HHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
TYSKAIEHVLRKAELLASTPSA
HHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362