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The map label for this gene is trmE

Identifier: 15618747

GI number: 15618747

Start: 947778

End: 949106

Strand: Reverse

Name: trmE

Synonym: CPn0838

Alternate gene names: 15618747

Gene position: 949106-947778 (Counterclockwise)

Preceding gene: 15618753

Following gene: 15618746

Centisome position: 77.15

GC content: 43.49

Gene sequence:

>1329_bases
ATGCTAAAGCACGATACCATTGCTGCCATTGCCACTCCTCCAGGGGAAGGAAGTATTGCTGTTGTACGACTCTCTGGCCC
ACAAGCGATTGTCATTGCCGATCGTATTTTTTCTGGATCTGTGGCTAGCTTTGCCTCCCATACGATACATCTTGGACAAG
TCATTTTTGAAGAGACATTAATTGACCAAGCTCTTCTTCTTCTAATGCGCTCTCCTCGCTCCTTCACTGGAGAAGATGTA
GTCGAATTTCAGTGTCATGGAGGATTTTTCGCTTGCTCCCAAATTTTAGACGCTTTGATTGCTTTAGGCGCCCGTCCTGC
ACTCCCTGGAGAGTTTTCTCAACGAGCGTTTCTAAATGGAAAAATTGACCTGGTTCAAGCAGAGGCAATCCAAAATCTCA
TTGTTGCGGAAAATATAGACGCCTTTCGGATTGCTCAAACGCATTTTCAAGGAAATTTTTCTAAGAAAATTCAAGAAATA
CATACTCTGATTATCGAAGCATTGGCGTTTTTGGAAGTCCTAGCCGACTTCCCTGAAGAAGAACAACCAGACCTCCTCGT
CCCTCAAGAAAAAATTCAAAATGCTCTGCATATCGTGGAAGATTTTATTTCCAGTTTTGATGAGGGGCAGAGGCTTGCTC
AGGGAACAAGTTTGATCCTTGCAGGGAAACCTAACGTAGGGAAATCCTCCCTACTCAATGCGCTTCTTCAGAAAAATCGG
GCGATTGTAACACATATTCCTGGAACCACTCGTGATATCTTAGAGGAGCAGTGGCTCTTGCAAGGCAAACGCATCCGACT
GCTAGACACAGCAGGACAAAGAACAACAGACAATGACATTGAAAAAGAGGGCATTGAACGAGCTCTTTCTGCTATGGAAG
AAGCGGATGGGATCCTCTGGGTAATAGATGCGACGCAACCTCTAGAAGATCTTCCTAAAATTCTTTTTACAAAACCTTCA
TTCCTCCTATGGAATAAAGCTGACCTCACCCCTCCTCCATTCCTTGACACTTCGCTCCCTCAATTTGCGATCTCAGCAAA
AACTGGTGAGGGACTTACTCAAGTGAAACAAGCTTTAATCCAATGGATGCAAAAACAAGAGGCTGGAAAAACCTCTAAAG
TGTTTTTAGTTTCTTCTCGCCATCACATGATCTTACAAGAGGTGGCTCGCTGTCTGAAAGAAGCGCAGCAAAACCTCTAT
CTGCAACCTCCAGAAATCATTGCTTTGGAGTTAAGAGAGGCACTTCACTCGATTGGGATGCTTTCTGGCAAAGAAGTTAC
TGAAAGTATTTTAGGGGAAATTTTTAGTAAATTTTGCATTGGAAAATAG

Upstream 100 bases:

>100_bases
TTCCAATTGAATTAGTCTTAATATTTATCCAAACTCCTTTCACCTCTTTAATCTAAAACTTGGTATTCTATCCGAAAGTT
CCTTTAACTTTTCTATTTTT

Downstream 100 bases:

>100_bases
CTCACCATGAAGCAATTTATTCTCAGAACACTCAACGCGCTATTTCCCAATCCAAAACCATCTTTAGAAGGGTGGTCCTC
TCCCTTTCAACTACTTATTG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MLKHDTIAAIATPPGEGSIAVVRLSGPQAIVIADRIFSGSVASFASHTIHLGQVIFEETLIDQALLLLMRSPRSFTGEDV
VEFQCHGGFFACSQILDALIALGARPALPGEFSQRAFLNGKIDLVQAEAIQNLIVAENIDAFRIAQTHFQGNFSKKIQEI
HTLIIEALAFLEVLADFPEEEQPDLLVPQEKIQNALHIVEDFISSFDEGQRLAQGTSLILAGKPNVGKSSLLNALLQKNR
AIVTHIPGTTRDILEEQWLLQGKRIRLLDTAGQRTTDNDIEKEGIERALSAMEEADGILWVIDATQPLEDLPKILFTKPS
FLLWNKADLTPPPFLDTSLPQFAISAKTGEGLTQVKQALIQWMQKQEAGKTSKVFLVSSRHHMILQEVARCLKEAQQNLY
LQPPEIIALELREALHSIGMLSGKEVTESILGEIFSKFCIGK

Sequences:

>Translated_442_residues
MLKHDTIAAIATPPGEGSIAVVRLSGPQAIVIADRIFSGSVASFASHTIHLGQVIFEETLIDQALLLLMRSPRSFTGEDV
VEFQCHGGFFACSQILDALIALGARPALPGEFSQRAFLNGKIDLVQAEAIQNLIVAENIDAFRIAQTHFQGNFSKKIQEI
HTLIIEALAFLEVLADFPEEEQPDLLVPQEKIQNALHIVEDFISSFDEGQRLAQGTSLILAGKPNVGKSSLLNALLQKNR
AIVTHIPGTTRDILEEQWLLQGKRIRLLDTAGQRTTDNDIEKEGIERALSAMEEADGILWVIDATQPLEDLPKILFTKPS
FLLWNKADLTPPPFLDTSLPQFAISAKTGEGLTQVKQALIQWMQKQEAGKTSKVFLVSSRHHMILQEVARCLKEAQQNLY
LQPPEIIALELREALHSIGMLSGKEVTESILGEIFSKFCIGK
>Mature_442_residues
MLKHDTIAAIATPPGEGSIAVVRLSGPQAIVIADRIFSGSVASFASHTIHLGQVIFEETLIDQALLLLMRSPRSFTGEDV
VEFQCHGGFFACSQILDALIALGARPALPGEFSQRAFLNGKIDLVQAEAIQNLIVAENIDAFRIAQTHFQGNFSKKIQEI
HTLIIEALAFLEVLADFPEEEQPDLLVPQEKIQNALHIVEDFISSFDEGQRLAQGTSLILAGKPNVGKSSLLNALLQKNR
AIVTHIPGTTRDILEEQWLLQGKRIRLLDTAGQRTTDNDIEKEGIERALSAMEEADGILWVIDATQPLEDLPKILFTKPS
FLLWNKADLTPPPFLDTSLPQFAISAKTGEGLTQVKQALIQWMQKQEAGKTSKVFLVSSRHHMILQEVARCLKEAQQNLY
LQPPEIIALELREALHSIGMLSGKEVTESILGEIFSKFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=475, Percent_Identity=33.8947368421053, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI193082993, Length=460, Percent_Identity=33.695652173913, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI193082988, Length=502, Percent_Identity=31.8725099601594, Blast_Score=171, Evalue=2e-42,
Organism=Escherichia coli, GI2367268, Length=466, Percent_Identity=36.9098712446352, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI87082120, Length=158, Percent_Identity=35.4430379746835, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17507259, Length=450, Percent_Identity=31.7777777777778, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6323665, Length=495, Percent_Identity=32.9292929292929, Blast_Score=200, Evalue=3e-52,
Organism=Drosophila melanogaster, GI45550813, Length=473, Percent_Identity=30.8668076109937, Blast_Score=174, Evalue=9e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_CHLPN (Q9Z768)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   D86595
- PIR:   E72029
- RefSeq:   NP_225033.1
- RefSeq:   NP_300895.1
- RefSeq:   NP_445568.1
- RefSeq:   NP_877139.1
- ProteinModelPortal:   Q9Z768
- SMR:   Q9Z768
- GeneID:   1467546
- GeneID:   894670
- GeneID:   919600
- GeneID:   963508
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP1031
- KEGG:   cpn:CPn0838
- KEGG:   cpt:CpB0867
- TIGR:   CP_1031
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- PhylomeDB:   Q9Z768
- ProtClustDB:   PRK05291
- BioCyc:   CPNE115711:CP_1031-MONOMER
- BioCyc:   CPNE115713:CPN0838-MONOMER
- BioCyc:   CPNE138677:CPJ0838-MONOMER
- BioCyc:   CPNE182082:CPB0867-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 48716; Mature: 48716

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: BINDING 23-23 BINDING 82-82 BINDING 121-121 BINDING 442-442

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKHDTIAAIATPPGEGSIAVVRLSGPQAIVIADRIFSGSVASFASHTIHLGQVIFEETL
CCCCCCEEEEECCCCCCCEEEEEECCCCEEEEECHHHCCCHHHHHHHHHHHHHHHHHHHH
IDQALLLLMRSPRSFTGEDVVEFQCHGGFFACSQILDALIALGARPALPGEFSQRAFLNG
HHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCC
KIDLVQAEAIQNLIVAENIDAFRIAQTHFQGNFSKKIQEIHTLIIEALAFLEVLADFPEE
CEEEEEHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EQPDLLVPQEKIQNALHIVEDFISSFDEGQRLAQGTSLILAGKPNVGKSSLLNALLQKNR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCC
AIVTHIPGTTRDILEEQWLLQGKRIRLLDTAGQRTTDNDIEKEGIERALSAMEEADGILW
EEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEEE
VIDATQPLEDLPKILFTKPSFLLWNKADLTPPPFLDTSLPQFAISAKTGEGLTQVKQALI
EEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHH
QWMQKQEAGKTSKVFLVSSRHHMILQEVARCLKEAQQNLYLQPPEIIALELREALHSIGM
HHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
LSGKEVTESILGEIFSKFCIGK
CCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKHDTIAAIATPPGEGSIAVVRLSGPQAIVIADRIFSGSVASFASHTIHLGQVIFEETL
CCCCCCEEEEECCCCCCCEEEEEECCCCEEEEECHHHCCCHHHHHHHHHHHHHHHHHHHH
IDQALLLLMRSPRSFTGEDVVEFQCHGGFFACSQILDALIALGARPALPGEFSQRAFLNG
HHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCC
KIDLVQAEAIQNLIVAENIDAFRIAQTHFQGNFSKKIQEIHTLIIEALAFLEVLADFPEE
CEEEEEHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EQPDLLVPQEKIQNALHIVEDFISSFDEGQRLAQGTSLILAGKPNVGKSSLLNALLQKNR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCC
AIVTHIPGTTRDILEEQWLLQGKRIRLLDTAGQRTTDNDIEKEGIERALSAMEEADGILW
EEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEEE
VIDATQPLEDLPKILFTKPSFLLWNKADLTPPPFLDTSLPQFAISAKTGEGLTQVKQALI
EEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHH
QWMQKQEAGKTSKVFLVSSRHHMILQEVARCLKEAQQNLYLQPPEIIALELREALHSIGM
HHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
LSGKEVTESILGEIFSKFCIGK
CCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362