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The map label for this gene is polA

Identifier: 15606966

GI number: 15606966

Start: 1384011

End: 1385735

Strand: Direct

Name: polA

Synonym: aq_1967

Alternate gene names: 15606966

Gene position: 1384011-1385735 (Clockwise)

Preceding gene: 15606959

Following gene: 15606967

Centisome position: 89.21

GC content: 44.58

Gene sequence:

>1725_bases
ATGGATTTTGAATACGTAACGGGAGAAGAGGGATTAAAAAAGGCAATAAAAAGGCTCGAAAATTCTCCATACCTTTACCT
GGATACGGAAACCACAGGAGACAGGATAAGGCTCGTACAAATCGGAGACGAAGAAAACACCTACGTTATTGACCTCTACG
AAATTCAGGATATAGAACCTCTGAGGAAATTAATAAACGAAAGGGGGATAGTAGGGCACAACCTTAAGTTCGATCTTAAG
TACCTCTACAGGTACGGGATATTTCCCTCGGCAACGTTTGACACTATGATAGCGAGCTACCTCCTCGGATACGAGAGACA
CTCCCTCAATCACATAGTTTCAAACCTACTCGGATATTCCATGGACAAGAGTTATCAGACTTCCGACTGGGGAGCGAGCG
TTCTGAGCGACGCTCAGCTCAAGTACGCTGCAAACGACGTTATAGTCCTCAGAGAACTCTTCCCTAAGATGAGGGACATG
TTAAACGAGCTAGACGCTGAGAGGGGAGAGGAACTGCTCAAGACTAGAACGGCAAAGATTTTCGATCTGAAGAGTCCCGT
AGCAATAGTGGAAATGGCTTTCGTAAGGGAAGTTGCAAAACTCGAGATAAACGGCTTTCCCGTGGACGTAGAAGAGCTAA
CCAACAAGTTAAAAGCTGTGGAAAGGGAAACCCAGAAGAGGATACAGGAGTTTTACATAAAGTACAGAGTTGACCCTCTC
TCTCCGAAACAGCTCGCCTCACTCCTGACGAAGAAGTTTAAACTGAACCTTCCCAAGACTCCTAAAGGGAACGTATCTAC
AGACGACAAGGCTCTTACTTCCTATCAGGACGTAGAACCCGTAAAACTCGTTCTGGAAATAAGAAAGCTTAAGAAGATCG
CGGACAAGTTAAAGGAGTTAAAAGAACACTTGAAGAACGGGAGAGTTTACCCGGAGTTCAAGCAAATAGGAGCTGTAACG
GGAAGGATGTCCTCCGCACACCCAAATATCCAGAACATACACAGGGATATGAGAGGAATTTTCAAGGCGGAGGAGGGAAA
TACTTTCGTCATTTCGGACTTTTCTCAGATAGAGCTCAGGATTGCGGCCGAATACGTAAAGGACCCGCTTATGCTGGACG
CCTTCAAAAAGGGAAAGGACATGCACAGGTACACCGCTTCAGTGGTACTCGGAAAGAAAGAGGAAGAAATAACAAAAGAG
GAGAGACAGCTCGCAAAAGCTATAAACTTCGGTCTCATATACGGCATTTCCGCTAAAGGGCTTGCAGAATACGCAAAGCT
TGGTTACGGCGTTGAAATTTCTTTAGAAGAAGCTCAGGTTTTGAGAGAGAGGTTTTTCAAGAACTTCAAAGCTTTCAAAG
AGTGGCACGACAGAGTTAAGAAAGAACTAAAGGAAAAGGGAGAGGTAAAAGGTCATACGCTTCTTGGAAGGAGATTTTCC
GCAAATACCTTTAACGACGCTGTAAATTACCCCATACAGGGAACGGGTGCGGACCTACTAAAACTGGCAGTTCTACTTTT
TGACGCAAACCTCCAGAAAAAGGGAATAGATGCAAAGCTCGTGAACCTCGTGCACGACGAGATAGTCGTAGAGTGCGAAA
AGGAAAAAGCGGAAGAAGTAAAAGAAATACTCGAAAAATCCATGAAAACGGCGGGAAAGATAATACTGAAAGAGGTTCCC
GTGGAAGTAGAAAGCGTTATAAACGAAAGGTGGACGAAAGATTAA

Upstream 100 bases:

>100_bases
GATTTCCTTACGAGCCTTATTTCTCCTTCTTCGGAATTTATACCATTTTCCAAGGTTTTTAAAAGCTCTTCATACTTCAC
CATAATATAAATTAAAAGCT

Downstream 100 bases:

>100_bases
TATAATCCTTATGCTTAAACTCAAGTTCAACGAAGAAGGACTCATTCCCGTAATCGCTCAGGACTACAGAACGGGCGAAG
TAAGAATGCTCGCCTACGCA

Product: DNA polymerase I (PolI)

Products: NA

Alternate protein names: POL I

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MDFEYVTGEEGLKKAIKRLENSPYLYLDTETTGDRIRLVQIGDEENTYVIDLYEIQDIEPLRKLINERGIVGHNLKFDLK
YLYRYGIFPSATFDTMIASYLLGYERHSLNHIVSNLLGYSMDKSYQTSDWGASVLSDAQLKYAANDVIVLRELFPKMRDM
LNELDAERGEELLKTRTAKIFDLKSPVAIVEMAFVREVAKLEINGFPVDVEELTNKLKAVERETQKRIQEFYIKYRVDPL
SPKQLASLLTKKFKLNLPKTPKGNVSTDDKALTSYQDVEPVKLVLEIRKLKKIADKLKELKEHLKNGRVYPEFKQIGAVT
GRMSSAHPNIQNIHRDMRGIFKAEEGNTFVISDFSQIELRIAAEYVKDPLMLDAFKKGKDMHRYTASVVLGKKEEEITKE
ERQLAKAINFGLIYGISAKGLAEYAKLGYGVEISLEEAQVLRERFFKNFKAFKEWHDRVKKELKEKGEVKGHTLLGRRFS
ANTFNDAVNYPIQGTGADLLKLAVLLFDANLQKKGIDAKLVNLVHDEIVVECEKEKAEEVKEILEKSMKTAGKIILKEVP
VEVESVINERWTKD

Sequences:

>Translated_574_residues
MDFEYVTGEEGLKKAIKRLENSPYLYLDTETTGDRIRLVQIGDEENTYVIDLYEIQDIEPLRKLINERGIVGHNLKFDLK
YLYRYGIFPSATFDTMIASYLLGYERHSLNHIVSNLLGYSMDKSYQTSDWGASVLSDAQLKYAANDVIVLRELFPKMRDM
LNELDAERGEELLKTRTAKIFDLKSPVAIVEMAFVREVAKLEINGFPVDVEELTNKLKAVERETQKRIQEFYIKYRVDPL
SPKQLASLLTKKFKLNLPKTPKGNVSTDDKALTSYQDVEPVKLVLEIRKLKKIADKLKELKEHLKNGRVYPEFKQIGAVT
GRMSSAHPNIQNIHRDMRGIFKAEEGNTFVISDFSQIELRIAAEYVKDPLMLDAFKKGKDMHRYTASVVLGKKEEEITKE
ERQLAKAINFGLIYGISAKGLAEYAKLGYGVEISLEEAQVLRERFFKNFKAFKEWHDRVKKELKEKGEVKGHTLLGRRFS
ANTFNDAVNYPIQGTGADLLKLAVLLFDANLQKKGIDAKLVNLVHDEIVVECEKEKAEEVKEILEKSMKTAGKIILKEVP
VEVESVINERWTKD
>Mature_574_residues
MDFEYVTGEEGLKKAIKRLENSPYLYLDTETTGDRIRLVQIGDEENTYVIDLYEIQDIEPLRKLINERGIVGHNLKFDLK
YLYRYGIFPSATFDTMIASYLLGYERHSLNHIVSNLLGYSMDKSYQTSDWGASVLSDAQLKYAANDVIVLRELFPKMRDM
LNELDAERGEELLKTRTAKIFDLKSPVAIVEMAFVREVAKLEINGFPVDVEELTNKLKAVERETQKRIQEFYIKYRVDPL
SPKQLASLLTKKFKLNLPKTPKGNVSTDDKALTSYQDVEPVKLVLEIRKLKKIADKLKELKEHLKNGRVYPEFKQIGAVT
GRMSSAHPNIQNIHRDMRGIFKAEEGNTFVISDFSQIELRIAAEYVKDPLMLDAFKKGKDMHRYTASVVLGKKEEEITKE
ERQLAKAINFGLIYGISAKGLAEYAKLGYGVEISLEEAQVLRERFFKNFKAFKEWHDRVKKELKEKGEVKGHTLLGRRFS
ANTFNDAVNYPIQGTGADLLKLAVLLFDANLQKKGIDAKLVNLVHDEIVVECEKEKAEEVKEILEKSMKTAGKIILKEVP
VEVESVINERWTKD

Specific function: In Addition To Polymerase Activity, This DNA Polymerase Exhibits 3' To 5' And 5' To 3' Exonuclease Activity. It Is Able To Utilize Nicked Circular Duplex DNA As A Template And Can Unwind The Parental DNA Strand From Its Template. [C]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 3'-5' exonuclease domain

Homologues:

Organism=Homo sapiens, GI112420958, Length=439, Percent_Identity=27.3348519362187, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI139394648, Length=281, Percent_Identity=24.9110320284698, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1790294, Length=629, Percent_Identity=33.0683624801272, Blast_Score=225, Evalue=5e-60,
Organism=Caenorhabditis elegans, GI86563272, Length=358, Percent_Identity=25.4189944134078, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI17933644, Length=420, Percent_Identity=26.6666666666667, Blast_Score=128, Evalue=1e-29,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): DPO1_AQUAE (O67779)

Other databases:

- EMBL:   AE000657
- PIR:   G70468
- RefSeq:   NP_214348.1
- ProteinModelPortal:   O67779
- SMR:   O67779
- GeneID:   1193676
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1967
- NMPDR:   fig|224324.1.peg.1363
- HOGENOM:   HBG507671
- OMA:   NLVHDEI
- ProtClustDB:   PRK14975
- BioCyc:   AAEO224324:AQ_1967-MONOMER
- BRENDA:   2.7.7.7
- GO:   GO:0005622
- InterPro:   IPR002562
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR002298
- InterPro:   IPR012337
- PRINTS:   PR00868
- SMART:   SM00474
- SMART:   SM00482

Pfam domain/function: PF01612 3_5_exonuc; PF00476 DNA_pol_A; SSF53098 RNaseH_fold

EC number: =2.7.7.7

Molecular weight: Translated: 65834; Mature: 65834

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFEYVTGEEGLKKAIKRLENSPYLYLDTETTGDRIRLVQIGDEENTYVIDLYEIQDIEP
CCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCCEEEEEEEECCCHHH
LRKLINERGIVGHNLKFDLKYLYRYGIFPSATFDTMIASYLLGYERHSLNHIVSNLLGYS
HHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
MDKSYQTSDWGASVLSDAQLKYAANDVIVLRELFPKMRDMLNELDAERGEELLKTRTAKI
CCCCCCCCCHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDLKSPVAIVEMAFVREVAKLEINGFPVDVEELTNKLKAVERETQKRIQEFYIKYRVDPL
EECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SPKQLASLLTKKFKLNLPKTPKGNVSTDDKALTSYQDVEPVKLVLEIRKLKKIADKLKEL
CHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
KEHLKNGRVYPEFKQIGAVTGRMSSAHPNIQNIHRDMRGIFKAEEGNTFVISDFSQIELR
HHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEECCCCCEEEEECHHHHHHH
IAAEYVKDPLMLDAFKKGKDMHRYTASVVLGKKEEEITKEERQLAKAINFGLIYGISAKG
HHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHEEECCHHH
LAEYAKLGYGVEISLEEAQVLRERFFKNFKAFKEWHDRVKKELKEKGEVKGHTLLGRRFS
HHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
ANTFNDAVNYPIQGTGADLLKLAVLLFDANLQKKGIDAKLVNLVHDEIVVECEKEKAEEV
CCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KEILEKSMKTAGKIILKEVPVEVESVINERWTKD
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MDFEYVTGEEGLKKAIKRLENSPYLYLDTETTGDRIRLVQIGDEENTYVIDLYEIQDIEP
CCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCCEEEEEEEECCCHHH
LRKLINERGIVGHNLKFDLKYLYRYGIFPSATFDTMIASYLLGYERHSLNHIVSNLLGYS
HHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
MDKSYQTSDWGASVLSDAQLKYAANDVIVLRELFPKMRDMLNELDAERGEELLKTRTAKI
CCCCCCCCCHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDLKSPVAIVEMAFVREVAKLEINGFPVDVEELTNKLKAVERETQKRIQEFYIKYRVDPL
EECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SPKQLASLLTKKFKLNLPKTPKGNVSTDDKALTSYQDVEPVKLVLEIRKLKKIADKLKEL
CHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
KEHLKNGRVYPEFKQIGAVTGRMSSAHPNIQNIHRDMRGIFKAEEGNTFVISDFSQIELR
HHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEECCCCCEEEEECHHHHHHH
IAAEYVKDPLMLDAFKKGKDMHRYTASVVLGKKEEEITKEERQLAKAINFGLIYGISAKG
HHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHEEECCHHH
LAEYAKLGYGVEISLEEAQVLRERFFKNFKAFKEWHDRVKKELKEKGEVKGHTLLGRRFS
HHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
ANTFNDAVNYPIQGTGADLLKLAVLLFDANLQKKGIDAKLVNLVHDEIVVECEKEKAEEV
CCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KEILEKSMKTAGKIILKEVPVEVESVINERWTKD
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320