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The map label for this gene is argF

Identifier: 15606793

GI number: 15606793

Start: 1203752

End: 1204672

Strand: Direct

Name: argF

Synonym: aq_1711

Alternate gene names: 15606793

Gene position: 1203752-1204672 (Clockwise)

Preceding gene: 15606784

Following gene: 15606796

Centisome position: 77.59

GC content: 45.49

Gene sequence:

>921_bases
ATGAAAAGGGACTTTGTAGACCTGTGGGATTTAAGTCCCAAAGAGGCATGGGAGATAGTAAAAAAAACCCTGAAAGTTAA
AAAGGGAGAAGAAGAACTTGGAAAACCGCTTTCCGGGAAAACGATAGCCCTTCTCTTCACAAAGCCCTCAACGAGGACAA
GGGTTTCCTTTGAAGTGGGAATTTATCAGCTCGGCGGGAATTCCTTATTTTTTCAAGAGAAAGAGCTACAAGTTTCCAGG
GGGGAGGACGTAAGAGATACCGCAAGAACACTGTCAAAATACGTTGACGGGGTGATAGTGAGAAATCACTCCCACACGTG
GTTGAAGGAGTTTGCAAATTTCGCGAGCGTTCCGGTTATAAACGCTTTGACCAACATGTCCCATCCCTGTCAGATACTGA
GTGATGTGTTCACACTTTACGAACACTACGGTGAGGAACTTAAAAACCTAAAGGTAGCCTACGTGGGGGACGGGAACAAC
GTGTGCAATACCTTAATGGTCGGGGCGGGGATGTTCGGACTGAAACTCTTCGTGGCAACCCCTGAAGGTTACGAGCCAAA
TTCCTATTACTACAAAAAAGCACTTGAGTTTTCAAAGGAAAACGGCGGGAGCGTGGAACTAACTAACAACCCGGTGGAAT
CGGTAAAAGATGCGGATGTCGTTTACACGGACGTTTGGGTAAGCATGGGAGAAGAAAACAAAAACATAGAAGCGTTTTTA
CCTTATCAGGTAAACGAAAAACTCCTCTCTTTCGCAAAGAGTTCCGTCAAAGTAATGCACTGCCTCCCTGCAAAGAAAGG
GCAGGAGATTACCGAAGAGGTTTTTGAGAAGAACGCTGACTTTATATTCACGCAGGCGGAAAACAGACTGCACACACAGA
AGACACTCATGGAGTTCCTATTCAGAGAACCTCAAGCTTAG

Upstream 100 bases:

>100_bases
TTAAACTCCCTTTTTTCACCGAGCTTAGGAAAACCAAAAGCGTAGGTTTTTACCATGGATTTCTCCTCCTTTTAGAACTT
CTTATTTTAAAATATCTTTT

Downstream 100 bases:

>100_bases
CCTTCAGGGCTCCCGCCGACTTTATAGCTTGGAGAACCTGGATTATTTCTCGCGGTGTTGCTCCTATAGAGTTCAGAGCG
TCAACGAGTTCGGAAACAGT

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MKRDFVDLWDLSPKEAWEIVKKTLKVKKGEEELGKPLSGKTIALLFTKPSTRTRVSFEVGIYQLGGNSLFFQEKELQVSR
GEDVRDTARTLSKYVDGVIVRNHSHTWLKEFANFASVPVINALTNMSHPCQILSDVFTLYEHYGEELKNLKVAYVGDGNN
VCNTLMVGAGMFGLKLFVATPEGYEPNSYYYKKALEFSKENGGSVELTNNPVESVKDADVVYTDVWVSMGEENKNIEAFL
PYQVNEKLLSFAKSSVKVMHCLPAKKGQEITEEVFEKNADFIFTQAENRLHTQKTLMEFLFREPQA

Sequences:

>Translated_306_residues
MKRDFVDLWDLSPKEAWEIVKKTLKVKKGEEELGKPLSGKTIALLFTKPSTRTRVSFEVGIYQLGGNSLFFQEKELQVSR
GEDVRDTARTLSKYVDGVIVRNHSHTWLKEFANFASVPVINALTNMSHPCQILSDVFTLYEHYGEELKNLKVAYVGDGNN
VCNTLMVGAGMFGLKLFVATPEGYEPNSYYYKKALEFSKENGGSVELTNNPVESVKDADVVYTDVWVSMGEENKNIEAFL
PYQVNEKLLSFAKSSVKVMHCLPAKKGQEITEEVFEKNADFIFTQAENRLHTQKTLMEFLFREPQA
>Mature_306_residues
MKRDFVDLWDLSPKEAWEIVKKTLKVKKGEEELGKPLSGKTIALLFTKPSTRTRVSFEVGIYQLGGNSLFFQEKELQVSR
GEDVRDTARTLSKYVDGVIVRNHSHTWLKEFANFASVPVINALTNMSHPCQILSDVFTLYEHYGEELKNLKVAYVGDGNN
VCNTLMVGAGMFGLKLFVATPEGYEPNSYYYKKALEFSKENGGSVELTNNPVESVKDADVVYTDVWVSMGEENKNIEAFL
PYQVNEKLLSFAKSSVKVMHCLPAKKGQEITEEVFEKNADFIFTQAENRLHTQKTLMEFLFREPQA

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=312, Percent_Identity=38.7820512820513, Blast_Score=221, Evalue=6e-58,
Organism=Homo sapiens, GI18105007, Length=285, Percent_Identity=29.8245614035088, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1790703, Length=324, Percent_Identity=37.3456790123457, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI1786469, Length=323, Percent_Identity=35.6037151702786, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI2367364, Length=310, Percent_Identity=28.0645161290323, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI48994908, Length=332, Percent_Identity=26.5060240963855, Blast_Score=90, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI193204318, Length=309, Percent_Identity=27.5080906148867, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6322373, Length=317, Percent_Identity=31.8611987381703, Blast_Score=178, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6322331, Length=297, Percent_Identity=28.956228956229, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24642586, Length=269, Percent_Identity=27.5092936802974, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_AQUAE (O67607)

Other databases:

- EMBL:   AE000657
- PIR:   E70447
- RefSeq:   NP_214173.1
- ProteinModelPortal:   O67607
- SMR:   O67607
- PRIDE:   O67607
- GeneID:   1193322
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1711
- NMPDR:   fig|224324.1.peg.1188
- HOGENOM:   HBG579429
- OMA:   CVVTDTW
- PhylomeDB:   O67607
- ProtClustDB:   PRK00779
- BioCyc:   AAEO224324:AQ_1711-MONOMER
- BRENDA:   2.1.3.3
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 34724; Mature: 34724

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 101-101 BINDING 128-128

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRDFVDLWDLSPKEAWEIVKKTLKVKKGEEELGKPLSGKTIALLFTKPSTRTRVSFEVG
CCCCCCHHHCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEEEECCCCCEEEEEEEE
IYQLGGNSLFFQEKELQVSRGEDVRDTARTLSKYVDGVIVRNHSHTWLKEFANFASVPVI
EEEECCCEEEEEHHHHHCCCCCCHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHCCHHH
NALTNMSHPCQILSDVFTLYEHYGEELKNLKVAYVGDGNNVCNTLMVGAGMFGLKLFVAT
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCHHEEEEEEEC
PEGYEPNSYYYKKALEFSKENGGSVELTNNPVESVKDADVVYTDVWVSMGEENKNIEAFL
CCCCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHCCCCEEEEEHHEECCCCCCCEEEEE
PYQVNEKLLSFAKSSVKVMHCLPAKKGQEITEEVFEKNADFIFTQAENRLHTQKTLMEFL
CHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHH
FREPQA
HCCCCC
>Mature Secondary Structure
MKRDFVDLWDLSPKEAWEIVKKTLKVKKGEEELGKPLSGKTIALLFTKPSTRTRVSFEVG
CCCCCCHHHCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEEEECCCCCEEEEEEEE
IYQLGGNSLFFQEKELQVSRGEDVRDTARTLSKYVDGVIVRNHSHTWLKEFANFASVPVI
EEEECCCEEEEEHHHHHCCCCCCHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHCCHHH
NALTNMSHPCQILSDVFTLYEHYGEELKNLKVAYVGDGNNVCNTLMVGAGMFGLKLFVAT
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCHHEEEEEEEC
PEGYEPNSYYYKKALEFSKENGGSVELTNNPVESVKDADVVYTDVWVSMGEENKNIEAFL
CCCCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHCCCCEEEEEHHEECCCCCCCEEEEE
PYQVNEKLLSFAKSSVKVMHCLPAKKGQEITEEVFEKNADFIFTQAENRLHTQKTLMEFL
CHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHH
FREPQA
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320