Click here to switch to the map view.

The map label for this gene is aspS

Identifier: 15606775

GI number: 15606775

Start: 1177548

End: 1179359

Strand: Direct

Name: aspS

Synonym: aq_1677

Alternate gene names: 15606775

Gene position: 1177548-1179359 (Clockwise)

Preceding gene: 15606773

Following gene: 15606776

Centisome position: 75.91

GC content: 45.58

Gene sequence:

>1812_bases
ATGCTCACAGAGTACGGAGACTTCAAAAGAACCAAGTACTGCGGAGAGGTAAGCGAAGAAGACATCGGAAAGGAAGTCAA
GCTCGCGGGTTGGGTTCACAGAAAGAGACACCACGGCGGAGTAATATTCATAGACCTGAGGGACAGGGAGGGAATAGTTC
AGGTAGTAGTAGAAGAAAAGACAAATCCCGAGGCTTATGAGGTTGCGGACAAATTGAAGTCAGAGTACGTTATCGGAGTC
GTAGGAAAGGTGAGAAAAAGACCGGAAGGAACGGAAAATCCCAAGCTAAAAACAGGATACGTTGAAGTGGTTGCGGATAG
AATTCTCGTCTTCAACACGAGCGAAGCCCTACCCTTCCCAGTAGAAGAAGAAACACATGTATCAGAAGAGACGAAGTTAA
AGTACAGGTACATAGACCTTAGAAGGGAGAGTATGAAGAACAACCTCATATTCAGACACAGGGTTTACCAGATAACTAGA
AACTTCTTCACAAAAGAGGGTTTTATAGAAATAGAAACTCCCTTCCTTACAAAGTCAACTCCCGAAGGAGCGAGGGACTT
TTTAGTTCCTTCAAGGCTACACCCGGGCAAGTTTTACGCCCTTCCTCAATCTCCTCAGCTATTTAAACAAATACTAATGA
TTGCAGGGTTTGACAGGTACTTCCAGATAGTAAAGTGTTTCAGGGACGAGGACCTGAGGGCGGACAGACAGCCCGAATTT
ACCCAGATAGATTACGAGATGTCCTTCGTGAGCGAAGAAGAGGTAATGGATGTTGCGGAAAGACTTATCGCCACCCTCTT
TAAAGAACTCCTCGGAGTAGAACTAAAAACACCCTTTGAGAGGATTTCTTACAGGGAAGCTATGGAGAAGTACGGGACGG
ATAAGCCAGACAGGAGGTTCGGACTAGAACTTATAGAACTCACGGACATATTCAAAAACACAGCCTTTAAGGTGTTTAAA
AGCGTCGTTGAAGCGGGAGGGATAATAAAGGCTATAAACTTCAAAGGCTCAAATCTCTCAAGGAAGGAGATAGACGAACT
CACAAAGTTCGTGCAAAGTCTCGGTGCAAAGGGACTTGCGTGGATAAAGGTAGAAAAGGACAAACTCACATCACCGATAG
TGAAGTTCTTCACCGAAGAAGAAACTCAGAAACTCCTTGAAAGGACGAAAGCAGAACCCGGAGATGTGATACTCTTTTCC
GCGGACAAAAAGGAAATGGTTTACAAAATCCTCGGAAACCTGAGACTCCACCTCGGAAAGAAGTACAAGCTTATAGACGA
GAGCAAGTGGGACGTTTTCTGGATAGTTGATTTCCCTCTTATGGAGTGGGACGAAGAGGAAGAGAGGTTCGTATCACTTC
ACCACCCCTTCACCATGCCAAGGGAAGAGAACATTCCAAAACTAAAGGAAGCCTTGGAAGAGGAAGATTTAGAAAAGAAA
AAGGAAATTGTTCACTCGGTAAGGGCGAGGGCTTACGACATGGTCCTGAACGGAGAGGAAATAGGCGGAGGTTCTATACG
TATCCACAGAAGGGATATTCAGGAAGTTGTTTTCAAACTCCTCGGTATAGGTGAGGTGGAAGCTCAGGAGAAGTTCGGCT
TCCTTCTGGAAGCCCTCAAATACGGAGCACCTCCCCACGGTGGACTTGCCTTCGGGCTTGATAGGGTAGTGGCTTTGATG
CTCGGACTTGATTCTATAAGGGACACGATAGCCTTCCCGAAGACTCAAAGAGGTATATGTCCTCTTACGGGAGCGCCCGA
TTACGTGGACCCCAAACAACTCAAGGAACTTCACATAAAAGTTTTAGAATAA

Upstream 100 bases:

>100_bases
TTACCTCCCGTTTAATTTTCCTTTAATAATATTTCTTGAGTATAATTGTGTCAACCTTTTATACCTTTCGTGCACACTCC
CTCCACTATAATTAATTTCT

Downstream 100 bases:

>100_bases
AAGTATGGAGAAGAGAATTACGACACCTCTTACGGACGAAGTTGTGAAGTCCTTAAGGGCGGGAGACAAGGTTCTCATAA
CGGGCTACATTTATACCGCA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 603; Mature: 603

Protein sequence:

>603_residues
MLTEYGDFKRTKYCGEVSEEDIGKEVKLAGWVHRKRHHGGVIFIDLRDREGIVQVVVEEKTNPEAYEVADKLKSEYVIGV
VGKVRKRPEGTENPKLKTGYVEVVADRILVFNTSEALPFPVEEETHVSEETKLKYRYIDLRRESMKNNLIFRHRVYQITR
NFFTKEGFIEIETPFLTKSTPEGARDFLVPSRLHPGKFYALPQSPQLFKQILMIAGFDRYFQIVKCFRDEDLRADRQPEF
TQIDYEMSFVSEEEVMDVAERLIATLFKELLGVELKTPFERISYREAMEKYGTDKPDRRFGLELIELTDIFKNTAFKVFK
SVVEAGGIIKAINFKGSNLSRKEIDELTKFVQSLGAKGLAWIKVEKDKLTSPIVKFFTEEETQKLLERTKAEPGDVILFS
ADKKEMVYKILGNLRLHLGKKYKLIDESKWDVFWIVDFPLMEWDEEEERFVSLHHPFTMPREENIPKLKEALEEEDLEKK
KEIVHSVRARAYDMVLNGEEIGGGSIRIHRRDIQEVVFKLLGIGEVEAQEKFGFLLEALKYGAPPHGGLAFGLDRVVALM
LGLDSIRDTIAFPKTQRGICPLTGAPDYVDPKQLKELHIKVLE

Sequences:

>Translated_603_residues
MLTEYGDFKRTKYCGEVSEEDIGKEVKLAGWVHRKRHHGGVIFIDLRDREGIVQVVVEEKTNPEAYEVADKLKSEYVIGV
VGKVRKRPEGTENPKLKTGYVEVVADRILVFNTSEALPFPVEEETHVSEETKLKYRYIDLRRESMKNNLIFRHRVYQITR
NFFTKEGFIEIETPFLTKSTPEGARDFLVPSRLHPGKFYALPQSPQLFKQILMIAGFDRYFQIVKCFRDEDLRADRQPEF
TQIDYEMSFVSEEEVMDVAERLIATLFKELLGVELKTPFERISYREAMEKYGTDKPDRRFGLELIELTDIFKNTAFKVFK
SVVEAGGIIKAINFKGSNLSRKEIDELTKFVQSLGAKGLAWIKVEKDKLTSPIVKFFTEEETQKLLERTKAEPGDVILFS
ADKKEMVYKILGNLRLHLGKKYKLIDESKWDVFWIVDFPLMEWDEEEERFVSLHHPFTMPREENIPKLKEALEEEDLEKK
KEIVHSVRARAYDMVLNGEEIGGGSIRIHRRDIQEVVFKLLGIGEVEAQEKFGFLLEALKYGAPPHGGLAFGLDRVVALM
LGLDSIRDTIAFPKTQRGICPLTGAPDYVDPKQLKELHIKVLE
>Mature_603_residues
MLTEYGDFKRTKYCGEVSEEDIGKEVKLAGWVHRKRHHGGVIFIDLRDREGIVQVVVEEKTNPEAYEVADKLKSEYVIGV
VGKVRKRPEGTENPKLKTGYVEVVADRILVFNTSEALPFPVEEETHVSEETKLKYRYIDLRRESMKNNLIFRHRVYQITR
NFFTKEGFIEIETPFLTKSTPEGARDFLVPSRLHPGKFYALPQSPQLFKQILMIAGFDRYFQIVKCFRDEDLRADRQPEF
TQIDYEMSFVSEEEVMDVAERLIATLFKELLGVELKTPFERISYREAMEKYGTDKPDRRFGLELIELTDIFKNTAFKVFK
SVVEAGGIIKAINFKGSNLSRKEIDELTKFVQSLGAKGLAWIKVEKDKLTSPIVKFFTEEETQKLLERTKAEPGDVILFS
ADKKEMVYKILGNLRLHLGKKYKLIDESKWDVFWIVDFPLMEWDEEEERFVSLHHPFTMPREENIPKLKEALEEEDLEKK
KEIVHSVRARAYDMVLNGEEIGGGSIRIHRRDIQEVVFKLLGIGEVEAQEKFGFLLEALKYGAPPHGGLAFGLDRVVALM
LGLDSIRDTIAFPKTQRGICPLTGAPDYVDPKQLKELHIKVLE

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=613, Percent_Identity=38.9885807504078, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI194272210, Length=381, Percent_Identity=24.4094488188976, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI5031815, Length=381, Percent_Identity=24.4094488188976, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI45439306, Length=259, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=7e-17,
Organism=Homo sapiens, GI4758762, Length=285, Percent_Identity=25.9649122807018, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI226491502, Length=302, Percent_Identity=26.4900662251656, Blast_Score=74, Evalue=5e-13,
Organism=Escherichia coli, GI1788173, Length=602, Percent_Identity=49.3355481727575, Blast_Score=589, Evalue=1e-169,
Organism=Escherichia coli, GI1789256, Length=348, Percent_Identity=26.4367816091954, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1790571, Length=346, Percent_Identity=26.3005780346821, Blast_Score=104, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI32566633, Length=605, Percent_Identity=35.8677685950413, Blast_Score=334, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI71994340, Length=312, Percent_Identity=22.4358974358974, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17535925, Length=312, Percent_Identity=22.4358974358974, Blast_Score=93, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17535927, Length=312, Percent_Identity=22.4358974358974, Blast_Score=93, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17551876, Length=280, Percent_Identity=28.2142857142857, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71984122, Length=290, Percent_Identity=26.2068965517241, Blast_Score=81, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325153, Length=639, Percent_Identity=32.5508607198748, Blast_Score=263, Evalue=4e-71,
Organism=Saccharomyces cerevisiae, GI6323011, Length=286, Percent_Identity=31.4685314685315, Blast_Score=97, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6320242, Length=277, Percent_Identity=26.7148014440433, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321807, Length=294, Percent_Identity=26.1904761904762, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324256, Length=172, Percent_Identity=25, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24584738, Length=608, Percent_Identity=37.5, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI17136276, Length=574, Percent_Identity=24.7386759581882, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24640851, Length=307, Percent_Identity=27.3615635179153, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24640849, Length=307, Percent_Identity=27.3615635179153, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19921528, Length=294, Percent_Identity=29.2517006802721, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_AQUAE (O67589)

Other databases:

- EMBL:   AE000657
- PIR:   D70445
- RefSeq:   NP_214155.1
- ProteinModelPortal:   O67589
- SMR:   O67589
- GeneID:   1193305
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1677
- NMPDR:   fig|224324.1.peg.1170
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- PhylomeDB:   O67589
- ProtClustDB:   PRK00476
- BioCyc:   AAEO224324:AQ_1677-MONOMER
- BRENDA:   6.1.1.12
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 69730; Mature: 69730

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTEYGDFKRTKYCGEVSEEDIGKEVKLAGWVHRKRHHGGVIFIDLRDREGIVQVVVEEK
CCCCCCCCHHHHHCCCCCHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECC
TNPEAYEVADKLKSEYVIGVVGKVRKRPEGTENPKLKTGYVEVVADRILVFNTSEALPFP
CCCHHHHHHHHHHHCEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHCEEEEEECCCCCCCC
VEEETHVSEETKLKYRYIDLRRESMKNNLIFRHRVYQITRNFFTKEGFIEIETPFLTKST
CCCCCCCCHHHHEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
PEGARDFLVPSRLHPGKFYALPQSPQLFKQILMIAGFDRYFQIVKCFRDEDLRADRQPEF
CCCHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCC
TQIDYEMSFVSEEEVMDVAERLIATLFKELLGVELKTPFERISYREAMEKYGTDKPDRRF
EEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHH
GLELIELTDIFKNTAFKVFKSVVEAGGIIKAINFKGSNLSRKEIDELTKFVQSLGAKGLA
CHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEE
WIKVEKDKLTSPIVKFFTEEETQKLLERTKAEPGDVILFSADKKEMVYKILGNLRLHLGK
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHCCC
KYKLIDESKWDVFWIVDFPLMEWDEEEERFVSLHHPFTMPREENIPKLKEALEEEDLEKK
CEEEECCCCCCEEEEEECCCCCCCCHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
KEIVHSVRARAYDMVLNGEEIGGGSIRIHRRDIQEVVFKLLGIGEVEAQEKFGFLLEALK
HHHHHHHHHHHHHEEECCCCCCCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
YGAPPHGGLAFGLDRVVALMLGLDSIRDTIAFPKTQRGICPLTGAPDYVDPKQLKELHIK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
VLE
CCC
>Mature Secondary Structure
MLTEYGDFKRTKYCGEVSEEDIGKEVKLAGWVHRKRHHGGVIFIDLRDREGIVQVVVEEK
CCCCCCCCHHHHHCCCCCHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECC
TNPEAYEVADKLKSEYVIGVVGKVRKRPEGTENPKLKTGYVEVVADRILVFNTSEALPFP
CCCHHHHHHHHHHHCEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHCEEEEEECCCCCCCC
VEEETHVSEETKLKYRYIDLRRESMKNNLIFRHRVYQITRNFFTKEGFIEIETPFLTKST
CCCCCCCCHHHHEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
PEGARDFLVPSRLHPGKFYALPQSPQLFKQILMIAGFDRYFQIVKCFRDEDLRADRQPEF
CCCHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCC
TQIDYEMSFVSEEEVMDVAERLIATLFKELLGVELKTPFERISYREAMEKYGTDKPDRRF
EEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHH
GLELIELTDIFKNTAFKVFKSVVEAGGIIKAINFKGSNLSRKEIDELTKFVQSLGAKGLA
CHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEE
WIKVEKDKLTSPIVKFFTEEETQKLLERTKAEPGDVILFSADKKEMVYKILGNLRLHLGK
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHCCC
KYKLIDESKWDVFWIVDFPLMEWDEEEERFVSLHHPFTMPREENIPKLKEALEEEDLEKK
CEEEECCCCCCEEEEEECCCCCCCCHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
KEIVHSVRARAYDMVLNGEEIGGGSIRIHRRDIQEVVFKLLGIGEVEAQEKFGFLLEALK
HHHHHHHHHHHHHEEECCCCCCCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
YGAPPHGGLAFGLDRVVALMLGLDSIRDTIAFPKTQRGICPLTGAPDYVDPKQLKELHIK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
VLE
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320