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The map label for this gene is pyrF

Identifier: 15606705

GI number: 15606705

Start: 1115453

End: 1116148

Strand: Direct

Name: pyrF

Synonym: aq_1580

Alternate gene names: 15606705

Gene position: 1115453-1116148 (Clockwise)

Preceding gene: 15606704

Following gene: 15606706

Centisome position: 71.9

GC content: 43.97

Gene sequence:

>696_bases
ATGGCAAGAATTTGTGTTGCTCTGGACGTCCCCTGGGAAAGGGCTATAAAGATAGTAAAAGACCTTTACGACTTCTTTGA
AACATATCCCCTTATCTTAAAAATCGGACACAAGATTTACCTTGAAAAGGGCGGAGACGCTGTAAAGGAACTCAAGGAGG
ACTTTCCTTACGAGGTGTTCCTCGATTTAAAACTCCATGACATCCCTTCGGTTGTAGGACTTGCCGTTGAAAAGATCAGT
GAACTGGGAACGGATTACACCACGGTTCATCTGCTCGGCGGTCCGGAAATGGTAAAGGAAGCGGTAAAGAACAAGGGAAG
CATGAAAATCCTCGGTGTTACTATACTCACCTCGCACGACGAGAACTACATAAAGTTTTTAAAAAGCAACTTTGAAGATA
TAAAGAACTTCACCCTTTACCTCGCAAGAGTTGGTATAGAAAACGGCGTTGACGGAGTTGTTTGCTCAGGTGAAGAAGTT
GAGTTCTTAAAGAAGAACATAGAAAAGGACTTCATAGCGGTAGTTCCGGGAATAAGGATTAAAAAGGAAAAGAGGCACGA
CCAGAAGAGGGTTCTCACACCCGCGGAGGCAAAGAAAAGGGGAGCAGACGTTATAGTAATCGGAAGGGAAATTACAGAGA
GCAAGAATCCCGTCTACGTGGTAGAAAGGGCTCTAAAGATGATGGGAGAGATATGA

Upstream 100 bases:

>100_bases
GGGAGCTTGCCGTAAAGGCTTACAACGGGATAAGGGCGGATAACTGGGATTACGTAAAAAATGTTATGGAAAGGGCTAAA
AAGTACGTAAACTTATAGAA

Downstream 100 bases:

>100_bases
AAAAGCTTTTAATTTTATTAACCTTTGTCTCCTTTGTCTTTTCTAAGACCTTTTACTACGACGTTTACGTCTTCTTTTTC
AGAGTTGGAGAGATAAAAAT

Product: orotidine-5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MARICVALDVPWERAIKIVKDLYDFFETYPLILKIGHKIYLEKGGDAVKELKEDFPYEVFLDLKLHDIPSVVGLAVEKIS
ELGTDYTTVHLLGGPEMVKEAVKNKGSMKILGVTILTSHDENYIKFLKSNFEDIKNFTLYLARVGIENGVDGVVCSGEEV
EFLKKNIEKDFIAVVPGIRIKKEKRHDQKRVLTPAEAKKRGADVIVIGREITESKNPVYVVERALKMMGEI

Sequences:

>Translated_231_residues
MARICVALDVPWERAIKIVKDLYDFFETYPLILKIGHKIYLEKGGDAVKELKEDFPYEVFLDLKLHDIPSVVGLAVEKIS
ELGTDYTTVHLLGGPEMVKEAVKNKGSMKILGVTILTSHDENYIKFLKSNFEDIKNFTLYLARVGIENGVDGVVCSGEEV
EFLKKNIEKDFIAVVPGIRIKKEKRHDQKRVLTPAEAKKRGADVIVIGREITESKNPVYVVERALKMMGEI
>Mature_230_residues
ARICVALDVPWERAIKIVKDLYDFFETYPLILKIGHKIYLEKGGDAVKELKEDFPYEVFLDLKLHDIPSVVGLAVEKISE
LGTDYTTVHLLGGPEMVKEAVKNKGSMKILGVTILTSHDENYIKFLKSNFEDIKNFTLYLARVGIENGVDGVVCSGEEVE
FLKKNIEKDFIAVVPGIRIKKEKRHDQKRVLTPAEAKKRGADVIVIGREITESKNPVYVVERALKMMGEI

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=195, Percent_Identity=33.3333333333333, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_AQUAE (O67520)

Other databases:

- EMBL:   AE000657
- PIR:   F70436
- RefSeq:   NP_214085.1
- ProteinModelPortal:   O67520
- GeneID:   1193143
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1580
- NMPDR:   fig|224324.1.peg.1100
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- PhylomeDB:   O67520
- BioCyc:   AAEO224324:AQ_1580-MONOMER
- BRENDA:   4.1.1.23
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 26107; Mature: 25976

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 64-64 BINDING 9-9 BINDING 34-34 BINDING 117-117 BINDING 179-179 BINDING 188-188 BINDING 208-208 BINDING 209-209

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARICVALDVPWERAIKIVKDLYDFFETYPLILKIGHKIYLEKGGDAVKELKEDFPYEVF
CCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHCCCEEEE
LDLKLHDIPSVVGLAVEKISELGTDYTTVHLLGGPEMVKEAVKNKGSMKILGVTILTSHD
EEEEECCCHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHCCCCEEEEEEEEEECCC
ENYIKFLKSNFEDIKNFTLYLARVGIENGVDGVVCSGEEVEFLKKNIEKDFIAVVPGIRI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHCHHHHHHCCCCEE
KKEKRHDQKRVLTPAEAKKRGADVIVIGREITESKNPVYVVERALKMMGEI
EHHHHHHHHHCCCCHHHHHCCCCEEEECCHHCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARICVALDVPWERAIKIVKDLYDFFETYPLILKIGHKIYLEKGGDAVKELKEDFPYEVF
CEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHCCCEEEE
LDLKLHDIPSVVGLAVEKISELGTDYTTVHLLGGPEMVKEAVKNKGSMKILGVTILTSHD
EEEEECCCHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHCCCCEEEEEEEEEECCC
ENYIKFLKSNFEDIKNFTLYLARVGIENGVDGVVCSGEEVEFLKKNIEKDFIAVVPGIRI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHCHHHHHHCCCCEE
KKEKRHDQKRVLTPAEAKKRGADVIVIGREITESKNPVYVVERALKMMGEI
EHHHHHHHHHCCCCHHHHHCCCCEEEECCHHCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320