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The map label for this gene is speE2

Identifier: 15606549

GI number: 15606549

Start: 959557

End: 961038

Strand: Direct

Name: speE2

Synonym: aq_1350

Alternate gene names: 15606549

Gene position: 959557-961038 (Clockwise)

Preceding gene: 15606548

Following gene: 15606550

Centisome position: 61.85

GC content: 42.51

Gene sequence:

>1482_bases
ATGAGTGTTTCTACAGCTTTAAAGCTCTGTATTTTCTTTACTGGTTTTGCGGGAATAGTTGCCGAGTACTCCCTTGCCAC
TCTGGCTACTTACCTCCTCGGAAACGCGGTTCTCCAGTGGAGCGTGGTGATTTCCATCTTCCTGCTTTCTATGGGCCTTG
GCTCTCACGCGAGCAGGTATATTCCTGATGATAAAACACCTCTCGCTTTTGTCCTCGCGGAACTCTTCCTCTCCCTCCTC
GTTCCCTTTTCCGTTCCCATAGCCTATCACTTCGCAAATAACTTCCTTCACCTCCAGACGGTTATTTACGGACTTTCCTT
CGTTATAGGGAGCTTAATAGGTCTGGAAATTCCCCTGGCTGTTCGTATAAACAACATGTACGAAGAACTCAAGGTAAATA
TATCTTCCGTGCTCGAGAAGGATTACCTTGGCTCAGTTCCAGCGGGACTACTTTACGCATACCTTTTTCTCCCTAAACTC
GGTCTTCCCCTAACGGCTATTCTCGCGGGATTTTTCAACTTAATTTCTGCATTTTTACTGGTAAAGGTATTAAAACCGAA
AAAGTTTTTAAAGTTTTTGGCGATTTTTACTTTCTTTTTACTCGCAACTTACGCAGTTGGACACAAGAGGATAACACTCT
ACGAAGAGCAGAAGTTTTACGGAGAGGAGATAATCCACTTTGAGCAAACCCCTTACCAGAAGATAGTTTTAACACGGTTT
GGAAAACACTACTCTTTATACTTGGACGGACACCTCCAGTTCTCAACACTTGATGAAAAACGCTATCACGAGACGCTCGT
TCACGTTCCCGCCTCTTTTTTAAAAAGATATGAAAAGGCTCTAATACTCGGCGGAGGGGACGGACTGGCTTTAAGAGAAC
TCAGGAAATACCCTTTCGGGGAAATACACCTCGTTGATCTGGACCCTAAAATGATTGAGTTTTCAAAAAAGAATCTCGTT
ATGAGGAAAATTAACGAAAACAGCTTTTACGACACGAGGTTAAAGGTATTTTCGGAGGACGCCTTTAACTTTGTAAAAAA
GACAAAAGAGAAGTATGATTTCGTGATTGTGGACCTCATAGACCCCAGAACCCCATCAAGTGCGAGAGTTTACTCCCTGG
AGTTTTACATGTCTTTGAAAAACAAACTAAAAGAAGACGGCATTTTCATAACTCAGGCGGGAGACACTTTCTACAAGAGG
GAGGTGTTCTGTTCCATTCTAAAAACTATTAAGAAGGCGGGATTTTACGCGTACCCTTTAGTTGTGTACATCCCTACCTT
TGGCGAGTGGGGGATGGTTATAGGGAGTAAAGAGCCTTTAAACTTTGAGAACTTTGAACTCAAGGAAAAAACCGAGTTCC
TGAACAGGGAAAGGGCTTTAGCCTTTTACACACTCGGGAAGAGTTTGGAGTGCCCGAATGTGGAAGTAAACACTTTACTG
AAACCCGTGCTTATTTATTATTACTACAAAATACAAAATTAA

Upstream 100 bases:

>100_bases
TTATTCTTTCCTCAGAGGTTTACGAGAATAACCTTAGTGCCTCCCTTATACTGAGTGCTACATTCATAGCGAGTGCCTTC
ATAACCCTTGCGGTTATGGG

Downstream 100 bases:

>100_bases
CACTTTTAAACTAAATTAATATAACATGGAAAAGGTATTGGCAGAGGAAAGGATTGACCTCGGAAGATACGACGAGAGAT
TTTACGCAATTGTGGGAAGG

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase 2; PAPT 2; SPDSY 2

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSVSTALKLCIFFTGFAGIVAEYSLATLATYLLGNAVLQWSVVISIFLLSMGLGSHASRYIPDDKTPLAFVLAELFLSLL
VPFSVPIAYHFANNFLHLQTVIYGLSFVIGSLIGLEIPLAVRINNMYEELKVNISSVLEKDYLGSVPAGLLYAYLFLPKL
GLPLTAILAGFFNLISAFLLVKVLKPKKFLKFLAIFTFFLLATYAVGHKRITLYEEQKFYGEEIIHFEQTPYQKIVLTRF
GKHYSLYLDGHLQFSTLDEKRYHETLVHVPASFLKRYEKALILGGGDGLALRELRKYPFGEIHLVDLDPKMIEFSKKNLV
MRKINENSFYDTRLKVFSEDAFNFVKKTKEKYDFVIVDLIDPRTPSSARVYSLEFYMSLKNKLKEDGIFITQAGDTFYKR
EVFCSILKTIKKAGFYAYPLVVYIPTFGEWGMVIGSKEPLNFENFELKEKTEFLNRERALAFYTLGKSLECPNVEVNTLL
KPVLIYYYYKIQN

Sequences:

>Translated_493_residues
MSVSTALKLCIFFTGFAGIVAEYSLATLATYLLGNAVLQWSVVISIFLLSMGLGSHASRYIPDDKTPLAFVLAELFLSLL
VPFSVPIAYHFANNFLHLQTVIYGLSFVIGSLIGLEIPLAVRINNMYEELKVNISSVLEKDYLGSVPAGLLYAYLFLPKL
GLPLTAILAGFFNLISAFLLVKVLKPKKFLKFLAIFTFFLLATYAVGHKRITLYEEQKFYGEEIIHFEQTPYQKIVLTRF
GKHYSLYLDGHLQFSTLDEKRYHETLVHVPASFLKRYEKALILGGGDGLALRELRKYPFGEIHLVDLDPKMIEFSKKNLV
MRKINENSFYDTRLKVFSEDAFNFVKKTKEKYDFVIVDLIDPRTPSSARVYSLEFYMSLKNKLKEDGIFITQAGDTFYKR
EVFCSILKTIKKAGFYAYPLVVYIPTFGEWGMVIGSKEPLNFENFELKEKTEFLNRERALAFYTLGKSLECPNVEVNTLL
KPVLIYYYYKIQN
>Mature_492_residues
SVSTALKLCIFFTGFAGIVAEYSLATLATYLLGNAVLQWSVVISIFLLSMGLGSHASRYIPDDKTPLAFVLAELFLSLLV
PFSVPIAYHFANNFLHLQTVIYGLSFVIGSLIGLEIPLAVRINNMYEELKVNISSVLEKDYLGSVPAGLLYAYLFLPKLG
LPLTAILAGFFNLISAFLLVKVLKPKKFLKFLAIFTFFLLATYAVGHKRITLYEEQKFYGEEIIHFEQTPYQKIVLTRFG
KHYSLYLDGHLQFSTLDEKRYHETLVHVPASFLKRYEKALILGGGDGLALRELRKYPFGEIHLVDLDPKMIEFSKKNLVM
RKINENSFYDTRLKVFSEDAFNFVKKTKEKYDFVIVDLIDPRTPSSARVYSLEFYMSLKNKLKEDGIFITQAGDTFYKRE
VFCSILKTIKKAGFYAYPLVVYIPTFGEWGMVIGSKEPLNFENFELKEKTEFLNRERALAFYTLGKSLECPNVEVNTLLK
PVLIYYYYKIQN

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG4262

COG function: function code R; Predicted spermidine synthase with an N-terminal membrane domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=232, Percent_Identity=31.8965517241379, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1786312, Length=196, Percent_Identity=32.6530612244898, Blast_Score=104, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI212645616, Length=236, Percent_Identity=28.3898305084746, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25154106, Length=233, Percent_Identity=29.6137339055794, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325326, Length=227, Percent_Identity=29.9559471365639, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323175, Length=248, Percent_Identity=29.4354838709677, Blast_Score=93, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24645443, Length=257, Percent_Identity=29.9610894941634, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21355941, Length=198, Percent_Identity=30.3030303030303, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE2_AQUAE (O67365)

Other databases:

- EMBL:   AE000657
- PIR:   B70417
- RefSeq:   NP_213929.1
- ProteinModelPortal:   O67365
- GeneID:   1192803
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1350
- NMPDR:   fig|224324.1.peg.944
- HOGENOM:   HBG564037
- OMA:   YAKATRF
- PhylomeDB:   O67365
- ProtClustDB:   PRK03612
- BioCyc:   AAEO224324:AQ_1350-MONOMER
- BRENDA:   2.5.1.16
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 56479; Mature: 56348

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 233-233 BINDING 287-287 BINDING 306-306 BINDING 358-358 BINDING 361-361

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSTALKLCIFFTGFAGIVAEYSLATLATYLLGNAVLQWSVVISIFLLSMGLGSHASRY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
IPDDKTPLAFVLAELFLSLLVPFSVPIAYHFANNFLHLQTVIYGLSFVIGSLIGLEIPLA
CCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEE
VRINNMYEELKVNISSVLEKDYLGSVPAGLLYAYLFLPKLGLPLTAILAGFFNLISAFLL
EEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
VKVLKPKKFLKFLAIFTFFLLATYAVGHKRITLYEEQKFYGEEIIHFEQTPYQKIVLTRF
HHHHCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHCHHHEECCCCCHHHHHHHHC
GKHYSLYLDGHLQFSTLDEKRYHETLVHVPASFLKRYEKALILGGGDGLALRELRKYPFG
CCEEEEEEECEEEEEECCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHCCCC
EIHLVDLDPKMIEFSKKNLVMRKINENSFYDTRLKVFSEDAFNFVKKTKEKYDFVIVDLI
CEEEEECCCHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
DPRTPSSARVYSLEFYMSLKNKLKEDGIFITQAGDTFYKREVFCSILKTIKKAGFYAYPL
CCCCCCCCEEEEHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEE
VVYIPTFGEWGMVIGSKEPLNFENFELKEKTEFLNRERALAFYTLGKSLECPNVEVNTLL
EEEEECCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHH
KPVLIYYYYKIQN
HHHHHHHEEEECC
>Mature Secondary Structure 
SVSTALKLCIFFTGFAGIVAEYSLATLATYLLGNAVLQWSVVISIFLLSMGLGSHASRY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
IPDDKTPLAFVLAELFLSLLVPFSVPIAYHFANNFLHLQTVIYGLSFVIGSLIGLEIPLA
CCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEE
VRINNMYEELKVNISSVLEKDYLGSVPAGLLYAYLFLPKLGLPLTAILAGFFNLISAFLL
EEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
VKVLKPKKFLKFLAIFTFFLLATYAVGHKRITLYEEQKFYGEEIIHFEQTPYQKIVLTRF
HHHHCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHCHHHEECCCCCHHHHHHHHC
GKHYSLYLDGHLQFSTLDEKRYHETLVHVPASFLKRYEKALILGGGDGLALRELRKYPFG
CCEEEEEEECEEEEEECCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHCCCC
EIHLVDLDPKMIEFSKKNLVMRKINENSFYDTRLKVFSEDAFNFVKKTKEKYDFVIVDLI
CEEEEECCCHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
DPRTPSSARVYSLEFYMSLKNKLKEDGIFITQAGDTFYKREVFCSILKTIKKAGFYAYPL
CCCCCCCCEEEEHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEE
VVYIPTFGEWGMVIGSKEPLNFENFELKEKTEFLNRERALAFYTLGKSLECPNVEVNTLL
EEEEECCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHH
KPVLIYYYYKIQN
HHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320