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The map label for this gene is fdoH [H]

Identifier: 15606331

GI number: 15606331

Start: 723927

End: 724838

Strand: Direct

Name: fdoH [H]

Synonym: aq_1046

Alternate gene names: 15606331

Gene position: 723927-724838 (Clockwise)

Preceding gene: 15606330

Following gene: 15606332

Centisome position: 46.66

GC content: 48.36

Gene sequence:

>912_bases
ATGAGCACATTAACTACTGGTGGAACTGTAGGGCTTGGGATTAAGAGACTTTCCGCTTCTAAAGAACCAGACACTTCCGT
AAAAACTTATCCCGAACTTGCGATTCTCGTAGATGTCTCTTCTTGTATAGGTTGTAAAGCCTGTGAAGCGGCATGTCAGC
AATGGCACGATACAACTCCTCCCATACTTTCTCCAGAAGAAGTAATAAAGAGGAAAATAGCTGGTTACCAAACTCATCCC
GATCTGCTGCCGGAAACATTTATGCTAATGAAGTTTAAGGAAGGAGAAACAAGCCGTGGATTTACTTGGTTTATAACCAA
ATACCAATGTATGCACTGCAGGGAACCGGGATGTCTGATCGCCTGCCCCTCCCCCGGGGCGGTAATCCAGTACCAGAACG
GCGTGGTGGACTTCGACCACTCCAAGTGCATAGGTTGTAAAATGTGCTTGGTGGGCTGTCCCTTTGATATTCCGAGGTAC
GATAAGAATAACAAACCTTGGAAATGCAACTTCTGTATCGACAGGGTAAGTGCAGGTTTAGAACCAGCCTGCGTGAAGAC
CTGCCCAACCAACTGCCTCTCCTTCGGCTTTAAGGAGGATATGATAGAGAGGGGTAAGAAGATAGTGGAGAGATTAAAGG
AGAGGGGGTACCCGAACGCAACCCTCTACGATCCTCCGGGTGTCGGTGGAACGGGATACATTTACGTTCTTCCCCACGGT
GATCATGTGAAGGACTATCAGCTTCCCGAAAACCCGAGGGTGGATGCATCGATAGGTCTGTGGAAGGGTCCTCTGAAGAC
GATAGGAAGCATAGCCTTTTGGGGCACACTTCTGGGTGTGTTCCTTCAGTTGATACTCTGGGGACCGATAAAGGTCCTCG
GCGGAAAGGAAGAAAAAAGTGAGGAGGGTTAA

Upstream 100 bases:

>100_bases
TCATAACGCCCAACGTCTGGGATCCCAACTCTAGAACTCCTGAGTTTAAGGGCTTTCTTGCAAACATTGAAAAGGTGAAA
ACTTAAGGGAGGTGTAAAAA

Downstream 100 bases:

>100_bases
TCATGGCGGTAGAGTACAAAAAGGCTTACGAAGAGCAGATAGAGAGGTTCTCAGCCCTCGACAGGATTCTCCACTGGACG
ACAGCCCTCGCCTTCCTTTA

Product: formate dehydrogenase beta subunit

Products: CO2; NADH

Alternate protein names: Anaerobic formate dehydrogenase iron-sulfur subunit; Formate dehydrogenase-N subunit beta; FDH-N subunit beta [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MSTLTTGGTVGLGIKRLSASKEPDTSVKTYPELAILVDVSSCIGCKACEAACQQWHDTTPPILSPEEVIKRKIAGYQTHP
DLLPETFMLMKFKEGETSRGFTWFITKYQCMHCREPGCLIACPSPGAVIQYQNGVVDFDHSKCIGCKMCLVGCPFDIPRY
DKNNKPWKCNFCIDRVSAGLEPACVKTCPTNCLSFGFKEDMIERGKKIVERLKERGYPNATLYDPPGVGGTGYIYVLPHG
DHVKDYQLPENPRVDASIGLWKGPLKTIGSIAFWGTLLGVFLQLILWGPIKVLGGKEEKSEEG

Sequences:

>Translated_303_residues
MSTLTTGGTVGLGIKRLSASKEPDTSVKTYPELAILVDVSSCIGCKACEAACQQWHDTTPPILSPEEVIKRKIAGYQTHP
DLLPETFMLMKFKEGETSRGFTWFITKYQCMHCREPGCLIACPSPGAVIQYQNGVVDFDHSKCIGCKMCLVGCPFDIPRY
DKNNKPWKCNFCIDRVSAGLEPACVKTCPTNCLSFGFKEDMIERGKKIVERLKERGYPNATLYDPPGVGGTGYIYVLPHG
DHVKDYQLPENPRVDASIGLWKGPLKTIGSIAFWGTLLGVFLQLILWGPIKVLGGKEEKSEEG
>Mature_302_residues
STLTTGGTVGLGIKRLSASKEPDTSVKTYPELAILVDVSSCIGCKACEAACQQWHDTTPPILSPEEVIKRKIAGYQTHPD
LLPETFMLMKFKEGETSRGFTWFITKYQCMHCREPGCLIACPSPGAVIQYQNGVVDFDHSKCIGCKMCLVGCPFDIPRYD
KNNKPWKCNFCIDRVSAGLEPACVKTCPTNCLSFGFKEDMIERGKKIVERLKERGYPNATLYDPPGVGGTGYIYVLPHGD
HVKDYQLPENPRVDASIGLWKGPLKTIGSIAFWGTLLGVFLQLILWGPIKVLGGKEEKSEEG

Specific function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iro

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787749, Length=293, Percent_Identity=45.7337883959044, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI1790326, Length=277, Percent_Identity=45.1263537906137, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1789370, Length=226, Percent_Identity=30.9734513274336, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787122, Length=166, Percent_Identity=33.7349397590361, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787872, Length=174, Percent_Identity=31.6091954022989, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI87082180, Length=177, Percent_Identity=29.3785310734463, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI2367345, Length=168, Percent_Identity=29.7619047619048, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI226510944, Length=214, Percent_Identity=28.0373831775701, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787478, Length=100, Percent_Identity=36, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788811, Length=169, Percent_Identity=26.0355029585799, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787740, Length=121, Percent_Identity=29.7520661157025, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 33434; Mature: 33303

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: PS00198 4FE4S_FERREDOXIN ; PS01307 MOTA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
5.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLTTGGTVGLGIKRLSASKEPDTSVKTYPELAILVDVSSCIGCKACEAACQQWHDTTP
CCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEECHHHHCHHHHHHHHHHHCCCCC
PILSPEEVIKRKIAGYQTHPDLLPETFMLMKFKEGETSRGFTWFITKYQCMHCREPGCLI
CCCCHHHHHHHHHCCCCCCCCCCHHHEEEEEECCCCCCCCEEEEEEEEEEEECCCCCEEE
ACPSPGAVIQYQNGVVDFDHSKCIGCKMCLVGCPFDIPRYDKNNKPWKCNFCIDRVSAGL
ECCCCCCEEEECCCEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEHHHHHCCC
EPACVKTCPTNCLSFGFKEDMIERGKKIVERLKERGYPNATLYDPPGVGGTGYIYVLPHG
CCHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEEEECC
DHVKDYQLPENPRVDASIGLWKGPLKTIGSIAFWGTLLGVFLQLILWGPIKVLGGKEEKS
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCC
EEG
CCC
>Mature Secondary Structure 
STLTTGGTVGLGIKRLSASKEPDTSVKTYPELAILVDVSSCIGCKACEAACQQWHDTTP
CCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEECHHHHCHHHHHHHHHHHCCCCC
PILSPEEVIKRKIAGYQTHPDLLPETFMLMKFKEGETSRGFTWFITKYQCMHCREPGCLI
CCCCHHHHHHHHHCCCCCCCCCCHHHEEEEEECCCCCCCCEEEEEEEEEEEECCCCCEEE
ACPSPGAVIQYQNGVVDFDHSKCIGCKMCLVGCPFDIPRYDKNNKPWKCNFCIDRVSAGL
ECCCCCCEEEECCCEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEHHHHHCCC
EPACVKTCPTNCLSFGFKEDMIERGKKIVERLKERGYPNATLYDPPGVGGTGYIYVLPHG
CCHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEEEECC
DHVKDYQLPENPRVDASIGLWKGPLKTIGSIAFWGTLLGVFLQLILWGPIKVLGGKEEKS
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCC
EEG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1834669; 9097039; 9278503; 11884747 [H]