Click here to switch to the map view.

The map label for this gene is fdoI [H]

Identifier: 15606332

GI number: 15606332

Start: 724841

End: 725476

Strand: Direct

Name: fdoI [H]

Synonym: aq_1049

Alternate gene names: 15606332

Gene position: 724841-725476 (Clockwise)

Preceding gene: 15606331

Following gene: 15606333

Centisome position: 46.72

GC content: 51.1

Gene sequence:

>636_bases
ATGGCGGTAGAGTACAAAAAGGCTTACGAAGAGCAGATAGAGAGGTTCTCAGCCCTCGACAGGATTCTCCACTGGACGAC
AGCCCTCGCCTTCCTTTACTGTTTCCTGTCGGGGATAGGGATAGCCTACGCCAAGTTCCACTGGCTGCTGACCTTTCTCG
GTGGTGGTGAATTCGCCCGCTGGCTCCATCCGTGGGCCGGCGTCGTCTTTTCAATAGGCGCGGTCCTTATGATCCTCAAA
TGGGCAAGGGACATGGTGATCACTAGAGAGGACATAGTATGGCTGACGAAGATAAAAGCCTACATAAGCGGAAGGCACGA
GGAACTTCCCGAGGTCGGAAAATATAATGCAGGGCAGAAGCTATACGCCTGGGTGGTCTTCCTTAGCGCTATAGTCTTTT
TCCTTACGGGTATACTTATGTGGTTTCCGGAAAACTTCAGTATGGGACTAGTGCGCTGGTCTGTGGTTATACACGAGATA
ACTTTCATAATAGCCGGAGCCTTCACTATAATACACATCTACATGGCAACCGTCGGAGTTCCGGGCTCCATATGGGGCAT
GATAGGAGGAAAGGTATCAGCCACGTGGGCTAAGTTCCATCACCCCAAGTGGTACAGGGAGGTGGTAAAGAGGTAA

Upstream 100 bases:

>100_bases
CCTTTTGGGGCACACTTCTGGGTGTGTTCCTTCAGTTGATACTCTGGGGACCGATAAAGGTCCTCGGCGGAAAGGAAGAA
AAAAGTGAGGAGGGTTAATC

Downstream 100 bases:

>100_bases
GTGTCATGAGAAAGCTTAGGTGTTTGAGCAGTGGGATGCGCTCTGCGCCGTCCTTTTTTCTTTCTCAATAGCACAGGAGC
TCAAAACACATGCAGACATA

Product: formate dehydrogenase gamma subunit

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase cytochrome b556 subunit; FDH-Z subunit gamma; Formate dehydrogenase-O subunit gamma [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MAVEYKKAYEEQIERFSALDRILHWTTALAFLYCFLSGIGIAYAKFHWLLTFLGGGEFARWLHPWAGVVFSIGAVLMILK
WARDMVITREDIVWLTKIKAYISGRHEELPEVGKYNAGQKLYAWVVFLSAIVFFLTGILMWFPENFSMGLVRWSVVIHEI
TFIIAGAFTIIHIYMATVGVPGSIWGMIGGKVSATWAKFHHPKWYREVVKR

Sequences:

>Translated_211_residues
MAVEYKKAYEEQIERFSALDRILHWTTALAFLYCFLSGIGIAYAKFHWLLTFLGGGEFARWLHPWAGVVFSIGAVLMILK
WARDMVITREDIVWLTKIKAYISGRHEELPEVGKYNAGQKLYAWVVFLSAIVFFLTGILMWFPENFSMGLVRWSVVIHEI
TFIIAGAFTIIHIYMATVGVPGSIWGMIGGKVSATWAKFHHPKWYREVVKR
>Mature_210_residues
AVEYKKAYEEQIERFSALDRILHWTTALAFLYCFLSGIGIAYAKFHWLLTFLGGGEFARWLHPWAGVVFSIGAVLMILKW
ARDMVITREDIVWLTKIKAYISGRHEELPEVGKYNAGQKLYAWVVFLSAIVFFLTGILMWFPENFSMGLVRWSVVIHEIT
FIIAGAFTIIHIYMATVGVPGSIWGMIGGKVSATWAKFHHPKWYREVVKR

Specific function: Allows to use formate as major electron donor during aerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1790325, Length=204, Percent_Identity=34.3137254901961, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787750, Length=204, Percent_Identity=38.2352941176471, Blast_Score=137, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 24297; Mature: 24166

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVEYKKAYEEQIERFSALDRILHWTTALAFLYCFLSGIGIAYAKFHWLLTFLGGGEFAR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
WLHPWAGVVFSIGAVLMILKWARDMVITREDIVWLTKIKAYISGRHEELPEVGKYNAGQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHCCCCCCHH
LYAWVVFLSAIVFFLTGILMWFPENFSMGLVRWSVVIHEITFIIAGAFTIIHIYMATVGV
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PGSIWGMIGGKVSATWAKFHHPKWYREVVKR
CHHHHHHHCCCHHHHHHHHCCCHHHHHHHCC
>Mature Secondary Structure 
AVEYKKAYEEQIERFSALDRILHWTTALAFLYCFLSGIGIAYAKFHWLLTFLGGGEFAR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
WLHPWAGVVFSIGAVLMILKWARDMVITREDIVWLTKIKAYISGRHEELPEVGKYNAGQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHCCCCCCHH
LYAWVVFLSAIVFFLTGILMWFPENFSMGLVRWSVVIHEITFIIAGAFTIIHIYMATVGV
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PGSIWGMIGGKVSATWAKFHHPKWYREVVKR
CHHHHHHHCCCHHHHHHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]