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The map label for this gene is bioB

Identifier: 15606288

GI number: 15606288

Start: 674673

End: 675671

Strand: Direct

Name: bioB

Synonym: aq_975

Alternate gene names: 15606288

Gene position: 674673-675671 (Clockwise)

Preceding gene: 15606287

Following gene: 15606289

Centisome position: 43.49

GC content: 44.64

Gene sequence:

>999_bases
ATGGACAAAGTTGAGAGGAGACTTTACGAGCTTTACGAGAAGGCTATCAATTACGAGCCCTTATCCAAGGAGGAAGCCCT
TTATATACTTGAGGTAGATGACATATACGTCCCCTTCCTCGTACACCTTGCCCAGAAGATAAAGAAACACTACTTTCCCG
AAAACGAAGTGGAGTTTTGCTCCATAATAAACGCAAAGAGCGGAGCTTGTTCCGAGGACTGCAAGTTCTGTGCACAATCA
AAGTACTACAAAACTCCCATAAATGTTTACAATCTCGTTCCCGTTGACGAGATGGTGGAAGGTGCGATAAGAGGTGTTGA
GTTCGGTGCGAATAGGTACTGTATAGTTTTAAGTGGAAAGAGTGCAACCAAGGAGGAAGTTGAAAGGATAACTGAAGCAG
TAAAGGAAATAAAAAATGAAGGACTCCCCATAAACGTGTGCGTGTCCGCGGGAACTCTGGACGAGGAAAGCCTTAAAAAA
CTAAAAGAGGCGGGAGTAAAGAGGATAAACCACAACCTAGAAACTTCTAGAAATTTCTTCAAGAACATAGTAACCACTCA
CACGTGGGAAGATAGGTATGAAACCATAAAGAGGATAAAAAAGGTAGGACTCTCAACATGCAGCGGTGGGATTTTTGGCA
TGGGAGAGAGTAACGAGGACAGAGTTGACATGGCCCTCACCTACAGGGAACTAGAGGTTGACTCCATACCCCTTAACTTC
CTGATGCCCATAGAGGGCACTCCTATGGAAAACGCCCCCGGAGTTGAGGTTATGGAAGCCCTGAAAATTATTGCCATGTT
CAGGTTCACTAACCCAAAGGCGGAACTCAGACTCTGCGGAGGAAGGGAACAAAACTTAAGAGATTTCCACGGAATGGCTA
CGCTCATGACCAACGCCATGATGGTTGGAGGATATCTCACGAGAGCGGGAAGGGACATAAAGAAGGATTATCAACTACTG
AAAGATTTGAAAGCTAAAAGAAAAGTAAGCGTGGAGTAA

Upstream 100 bases:

>100_bases
GAAACCCGTACGAAAAAGTTAAAATGTATGAAGTTTTTCACAACACAAACGTGTGGAAGGTTTACAACGAACTTGATTTA
GAACTTTGAGGAGGCACGTT

Downstream 100 bases:

>100_bases
TATTCCCTTCTCCTTTCCCTTCTTTTCACCTCAAAAATAAAAATTTTTCTCCTAAATTAATTCATAGGAGGAATTTATAT
GCCTTTGTGGGGAAGGTTCA

Product: biotin synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MDKVERRLYELYEKAINYEPLSKEEALYILEVDDIYVPFLVHLAQKIKKHYFPENEVEFCSIINAKSGACSEDCKFCAQS
KYYKTPINVYNLVPVDEMVEGAIRGVEFGANRYCIVLSGKSATKEEVERITEAVKEIKNEGLPINVCVSAGTLDEESLKK
LKEAGVKRINHNLETSRNFFKNIVTTHTWEDRYETIKRIKKVGLSTCSGGIFGMGESNEDRVDMALTYRELEVDSIPLNF
LMPIEGTPMENAPGVEVMEALKIIAMFRFTNPKAELRLCGGREQNLRDFHGMATLMTNAMMVGGYLTRAGRDIKKDYQLL
KDLKAKRKVSVE

Sequences:

>Translated_332_residues
MDKVERRLYELYEKAINYEPLSKEEALYILEVDDIYVPFLVHLAQKIKKHYFPENEVEFCSIINAKSGACSEDCKFCAQS
KYYKTPINVYNLVPVDEMVEGAIRGVEFGANRYCIVLSGKSATKEEVERITEAVKEIKNEGLPINVCVSAGTLDEESLKK
LKEAGVKRINHNLETSRNFFKNIVTTHTWEDRYETIKRIKKVGLSTCSGGIFGMGESNEDRVDMALTYRELEVDSIPLNF
LMPIEGTPMENAPGVEVMEALKIIAMFRFTNPKAELRLCGGREQNLRDFHGMATLMTNAMMVGGYLTRAGRDIKKDYQLL
KDLKAKRKVSVE
>Mature_332_residues
MDKVERRLYELYEKAINYEPLSKEEALYILEVDDIYVPFLVHLAQKIKKHYFPENEVEFCSIINAKSGACSEDCKFCAQS
KYYKTPINVYNLVPVDEMVEGAIRGVEFGANRYCIVLSGKSATKEEVERITEAVKEIKNEGLPINVCVSAGTLDEESLKK
LKEAGVKRINHNLETSRNFFKNIVTTHTWEDRYETIKRIKKVGLSTCSGGIFGMGESNEDRVDMALTYRELEVDSIPLNF
LMPIEGTPMENAPGVEVMEALKIIAMFRFTNPKAELRLCGGREQNLRDFHGMATLMTNAMMVGGYLTRAGRDIKKDYQLL
KDLKAKRKVSVE

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=288, Percent_Identity=32.6388888888889, Blast_Score=185, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6321725, Length=265, Percent_Identity=35.4716981132075, Blast_Score=180, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_AQUAE (O67104)

Other databases:

- EMBL:   AE000657
- PIR:   E70384
- RefSeq:   NP_213667.1
- HSSP:   P12996
- ProteinModelPortal:   O67104
- SMR:   O67104
- GeneID:   1193738
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_975
- NMPDR:   fig|224324.1.peg.682
- HOGENOM:   HBG405569
- OMA:   LEICSGG
- PhylomeDB:   O67104
- ProtClustDB:   CLSK2299571
- BioCyc:   AAEO224324:AQ_975-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 37771; Mature: 37771

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKVERRLYELYEKAINYEPLSKEEALYILEVDDIYVPFLVHLAQKIKKHYFPENEVEFC
CCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHH
SIINAKSGACSEDCKFCAQSKYYKTPINVYNLVPVDEMVEGAIRGVEFGANRYCIVLSGK
HHCCCCCCCCHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHEECCCEEEEEEECC
SATKEEVERITEAVKEIKNEGLPINVCVSAGTLDEESLKKLKEAGVKRINHNLETSRNFF
CCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
KNIVTTHTWEDRYETIKRIKKVGLSTCSGGIFGMGESNEDRVDMALTYRELEVDSIPLNF
HHHHHCCCHHHHHHHHHHHHHHCCHHCCCCEEECCCCCCCCCHHEEEHHHHEECCCCCEE
LMPIEGTPMENAPGVEVMEALKIIAMFRFTNPKAELRLCGGREQNLRDFHGMATLMTNAM
EECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHH
MVGGYLTRAGRDIKKDYQLLKDLKAKRKVSVE
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDKVERRLYELYEKAINYEPLSKEEALYILEVDDIYVPFLVHLAQKIKKHYFPENEVEFC
CCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHH
SIINAKSGACSEDCKFCAQSKYYKTPINVYNLVPVDEMVEGAIRGVEFGANRYCIVLSGK
HHCCCCCCCCHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHEECCCEEEEEEECC
SATKEEVERITEAVKEIKNEGLPINVCVSAGTLDEESLKKLKEAGVKRINHNLETSRNFF
CCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
KNIVTTHTWEDRYETIKRIKKVGLSTCSGGIFGMGESNEDRVDMALTYRELEVDSIPLNF
HHHHHCCCHHHHHHHHHHHHHHCCHHCCCCEEECCCCCCCCCHHEEEHHHHEECCCCCEE
LMPIEGTPMENAPGVEVMEALKIIAMFRFTNPKAELRLCGGREQNLRDFHGMATLMTNAM
EECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHH
MVGGYLTRAGRDIKKDYQLLKDLKAKRKVSVE
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320