Click here to switch to the map view.

The map label for this gene is tmk

Identifier: 15606283

GI number: 15606283

Start: 669174

End: 669761

Strand: Direct

Name: tmk

Synonym: aq_969

Alternate gene names: 15606283

Gene position: 669174-669761 (Clockwise)

Preceding gene: 15606281

Following gene: 15606286

Centisome position: 43.14

GC content: 41.16

Gene sequence:

>588_bases
ATGTTAATCGCTTTTGAGGGAATAGACGGTTCGGGAAAGACTACACAGGCGAAAAAACTGTATGAGTACCTTAAACAAAA
GGGCTACTTTGTGTCCCTTTACAGGGAGCCCGGCGGAACAAAAGTTGGAGAAGTATTAAGAGAAATTTTACTTACGGAAG
AGCTAGACGAAAGGACTGAACTCCTGCTCTTTGAAGCATCCCGCTCAAAACTCATTGAAGAAAAGATAATCCCCGACTTA
AAAAGGGATAAAGTAGTTATACTCGACAGGTTTGTCCTTTCAACGATAGCGTATCAGGGATACGGAAAAGGTCTGGACGT
AGAGTTTATAAAGAACTTAAACGAATTTGCGACAAGGGGTGTAAAACCCGATATAACCTTACTCCTTGACATCCCCGTGG
ATATAGCGCTGAGGAGACTGAAAGAAAAGAACAGGTTTGAAAATAAGGAATTTCTTGAAAAAGTGAGAAAGGGATTTTTA
GAACTTGCAAAGGAAGAGGAAAACGTGGTTGTAATAGATGCGAGTGGGGAAGAAGAGGAAGTGTTTAAGGAAATATTGAG
GGCTTTAAGTGGTGTACTCCGCGTTTAG

Upstream 100 bases:

>100_bases
GTATATCCGCACCGCCCCTAATCGTGGCTTGTATCTGCTCTCCTATACACCACCAATCACCTGCGAGGATTGAAGGAGCT
AAAAGTTTCATAATAGTTAT

Downstream 100 bases:

>100_bases
TTTGATGTAGTCGTACCCTATGTCGGAGGAGTAGCACACCCACTCGTACTCTCCTTCGTTTAGTTCCACAGTTATATCAA
CTTCCCTGTCTTCAATCAAG

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MLIAFEGIDGSGKTTQAKKLYEYLKQKGYFVSLYREPGGTKVGEVLREILLTEELDERTELLLFEASRSKLIEEKIIPDL
KRDKVVILDRFVLSTIAYQGYGKGLDVEFIKNLNEFATRGVKPDITLLLDIPVDIALRRLKEKNRFENKEFLEKVRKGFL
ELAKEEENVVVIDASGEEEEVFKEILRALSGVLRV

Sequences:

>Translated_195_residues
MLIAFEGIDGSGKTTQAKKLYEYLKQKGYFVSLYREPGGTKVGEVLREILLTEELDERTELLLFEASRSKLIEEKIIPDL
KRDKVVILDRFVLSTIAYQGYGKGLDVEFIKNLNEFATRGVKPDITLLLDIPVDIALRRLKEKNRFENKEFLEKVRKGFL
ELAKEEENVVVIDASGEEEEVFKEILRALSGVLRV
>Mature_195_residues
MLIAFEGIDGSGKTTQAKKLYEYLKQKGYFVSLYREPGGTKVGEVLREILLTEELDERTELLLFEASRSKLIEEKIIPDL
KRDKVVILDRFVLSTIAYQGYGKGLDVEFIKNLNEFATRGVKPDITLLLDIPVDIALRRLKEKNRFENKEFLEKVRKGFL
ELAKEEENVVVIDASGEEEEVFKEILRALSGVLRV

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family

Homologues:

Organism=Homo sapiens, GI42544174, Length=197, Percent_Identity=32.48730964467, Blast_Score=77, Evalue=9e-15,
Organism=Escherichia coli, GI1787340, Length=195, Percent_Identity=35.3846153846154, Blast_Score=113, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6322517, Length=189, Percent_Identity=30.1587301587302, Blast_Score=67, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28573735, Length=170, Percent_Identity=32.9411764705882, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KTHY_AQUAE (O67099)

Other databases:

- EMBL:   AE000657
- PIR:   H70383
- RefSeq:   NP_213662.1
- PDB:   2PBR
- PDBsum:   2PBR
- ProteinModelPortal:   O67099
- SMR:   O67099
- GeneID:   1193641
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_969
- NMPDR:   fig|224324.1.peg.677
- HOGENOM:   HBG626009
- OMA:   LFITLEG
- PhylomeDB:   O67099
- ProtClustDB:   CLSK2748394
- BioCyc:   AAEO224324:AQ_969-MONOMER
- BRENDA:   2.7.4.9
- HAMAP:   MF_00165
- InterPro:   IPR018095
- InterPro:   IPR018094
- PANTHER:   PTHR10344
- TIGRFAMs:   TIGR00041

Pfam domain/function: PF02223 Thymidylate_kin

EC number: =2.7.4.9

Molecular weight: Translated: 22370; Mature: 22370

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIAFEGIDGSGKTTQAKKLYEYLKQKGYFVSLYREPGGTKVGEVLREILLTEELDERTE
CEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
LLLFEASRSKLIEEKIIPDLKRDKVVILDRFVLSTIAYQGYGKGLDVEFIKNLNEFATRG
EEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
VKPDITLLLDIPVDIALRRLKEKNRFENKEFLEKVRKGFLELAKEEENVVVIDASGEEEE
CCCCEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHH
VFKEILRALSGVLRV
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MLIAFEGIDGSGKTTQAKKLYEYLKQKGYFVSLYREPGGTKVGEVLREILLTEELDERTE
CEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
LLLFEASRSKLIEEKIIPDLKRDKVVILDRFVLSTIAYQGYGKGLDVEFIKNLNEFATRG
EEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
VKPDITLLLDIPVDIALRRLKEKNRFENKEFLEKVRKGFLELAKEEENVVVIDASGEEEE
CCCCEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHH
VFKEILRALSGVLRV
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320