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The map label for this gene is ostA [H]

Identifier: 15606281

GI number: 15606281

Start: 666538

End: 668499

Strand: Direct

Name: ostA [H]

Synonym: aq_967

Alternate gene names: 15606281

Gene position: 666538-668499 (Clockwise)

Preceding gene: 15606280

Following gene: 15606283

Centisome position: 42.97

GC content: 40.37

Gene sequence:

>1962_bases
GTGGCTCTCTTAATACTTTTCTTTCTTCTAGGACTTTCCTTCTCCTTGGAAGTTTTCTCGGAAAAACTCGAAATTAAAGA
AAATGAAATAATAGCGGAAGGAGATGCGGAAGCTTACTACAAGAATTACTACATAAAGGCTAAGAAGATAAAACTTTTGC
GGGACAAGGACGAGGTTTACGCCTACGAAGACGTTTACATAAAAAACTTAAAAACGGGTTTTGAAATAAGGGGAAGTTTT
GCTTACGTGAATATGAAGAAAAACAAAGGTTACTTTCTGAACGCTAAAGGTAAGTTCAGAGAGTTTAACTTCGAGGCAAA
AAAGATTGAACAGCTGGATAAGGAAAGGTTTAAGGTTTACGAGGCCACGGTAACGACCTGCCCCTTAGATGATAAAGAAC
TTTACCTCTGTATATTCAGGGCGAATATAACGAAGGAAAGGGCTCTCATTTTCCACAACAGATTGAAGTTCTTTAAAGTT
CCCATTTTCTACCTGCCCGTTTACCTGATTCCCCTCGGGGGAAGGAAAACGGGCTTCCTCATGCCCACGATAGGGAGTTC
CACTTACAGTTCTTTTATATACATCCAGCCCTTCTTCTGGGCAATATCCCGCGACAAGGACATGACGATAACCGCGGACT
ACAGAAAGGATCAGATGAAGGGACTTTCCTTAGAGTACAGACAAGCCTTTGACGAAGAAAGCGACCTGATAACTCAATTT
TCTCTTTATAAGGAGGATAAAAAGAAGGGGGACTGGTGGAAAGGTAGGGAAATGTACAGGGAAAACAGGTTCAGGATATT
TATGAAATACAGGAAAAAACCTTTTAAGATAGGTGTGGAAGAACTCTCAGACCCTTACTACTACGAAGACATATCCTTTA
AACAGGAGGAAATCACGAAGCCCTACACGAAAACTTACCTGATTTACGAAAAGGAAACAAAAAACTACCTCTTTTACTTC
CAGACGGTTCGATACAGGGATTTAACGGAAGAGAAAAACAACGCTGTAAACCTCCTCCCTGAAGCCAGTTTCCACCTAAA
ACCCTTTTACTACAAAGGATTTAATTTCTCATTGGACTCTTCGTTTACGAATTTTTACAGGAATTACGACGGGAGCTTTA
TGCGTATTTCTCTGGAACCTACGGTGGGAAAATACTTTGAATTGTTCAAAGTCAGTAATTATTCTTCTTTGACCCTTATA
AACAGGTACTACCCGGATTCAGAAGAAGACAAAATCGTGAGTACTTTCAGGTTCAGACACAGCGTTCCCCAGTACTTTTC
TCTATCCTACGGAGGATTTAAATTCAAAAACTTCCTTGAGGGACTATACACCTTTTCTCCAAAGGATTACGAGGTTCAGA
TATTCGATTATCAGGATGAGCTCAACGAAGAGAACAATTTCCAGTTTACACTTGTAGGAAATCTATACAAAAACAGAAAG
CTTATGGATTACTTCCTGAACACAGGATACAACCTTCTTGGCTCTTACACTTTTCCCACAGACGGAAAGAAGATTGACAA
GAAACTCCTCCCCCTTTACTACAACGTCTCCGTATACCCTGTGGAAAACGTCAGGTTCTGGCAGGACGCGGTTTACGACT
TTAACCTCGGTGTCTTTGCAAGGAATGTAATCGGTACGGATATTTCCTGGAAGAATTTCTCCGTGGGGATTTCCAATTCT
GTGTACAAAAACAGTGAAAAGAAAGTTACAACGGATCAGATAGCTCTTAACCTCAAGTACAGCGGGGATAAGTTCTTTTC
AGGCATTTCACTAAATTACGACAGACTAGTAGATAAGGAGACTTACAGGAACGTTTACGTAGGCGTAAAAGGAAAGTGCT
GGAGTTTAGTGCTGGATTATAAGAGAAATTACTACAGGAACAGGGACGAGTACGTGGATCAGGTATTTTTGAGGTTCAAC
CTCTTCTTCAAAGAAGAGATAGAAATTCCTTTAAGACGTTAA

Upstream 100 bases:

>100_bases
TGACTACCGGAAAGTACAAGCAGGAAGAACTCAAGAAGGCTAACTTCAAACCCGATTACGTTTTTCACTCTCTCAAAGAA
TTAATGCGAGAAATCAAAAA

Downstream 100 bases:

>100_bases
ACCGAGACAGCGGAAAGCATAAGGCTTTTTAATTTCCTCGTCTGGGCTTCTATGTCCTTCGCGCCGAATATTCCCGATCC
TACAACTACAACGTCCGCTC

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 653; Mature: 652

Protein sequence:

>653_residues
MALLILFFLLGLSFSLEVFSEKLEIKENEIIAEGDAEAYYKNYYIKAKKIKLLRDKDEVYAYEDVYIKNLKTGFEIRGSF
AYVNMKKNKGYFLNAKGKFREFNFEAKKIEQLDKERFKVYEATVTTCPLDDKELYLCIFRANITKERALIFHNRLKFFKV
PIFYLPVYLIPLGGRKTGFLMPTIGSSTYSSFIYIQPFFWAISRDKDMTITADYRKDQMKGLSLEYRQAFDEESDLITQF
SLYKEDKKKGDWWKGREMYRENRFRIFMKYRKKPFKIGVEELSDPYYYEDISFKQEEITKPYTKTYLIYEKETKNYLFYF
QTVRYRDLTEEKNNAVNLLPEASFHLKPFYYKGFNFSLDSSFTNFYRNYDGSFMRISLEPTVGKYFELFKVSNYSSLTLI
NRYYPDSEEDKIVSTFRFRHSVPQYFSLSYGGFKFKNFLEGLYTFSPKDYEVQIFDYQDELNEENNFQFTLVGNLYKNRK
LMDYFLNTGYNLLGSYTFPTDGKKIDKKLLPLYYNVSVYPVENVRFWQDAVYDFNLGVFARNVIGTDISWKNFSVGISNS
VYKNSEKKVTTDQIALNLKYSGDKFFSGISLNYDRLVDKETYRNVYVGVKGKCWSLVLDYKRNYYRNRDEYVDQVFLRFN
LFFKEEIEIPLRR

Sequences:

>Translated_653_residues
MALLILFFLLGLSFSLEVFSEKLEIKENEIIAEGDAEAYYKNYYIKAKKIKLLRDKDEVYAYEDVYIKNLKTGFEIRGSF
AYVNMKKNKGYFLNAKGKFREFNFEAKKIEQLDKERFKVYEATVTTCPLDDKELYLCIFRANITKERALIFHNRLKFFKV
PIFYLPVYLIPLGGRKTGFLMPTIGSSTYSSFIYIQPFFWAISRDKDMTITADYRKDQMKGLSLEYRQAFDEESDLITQF
SLYKEDKKKGDWWKGREMYRENRFRIFMKYRKKPFKIGVEELSDPYYYEDISFKQEEITKPYTKTYLIYEKETKNYLFYF
QTVRYRDLTEEKNNAVNLLPEASFHLKPFYYKGFNFSLDSSFTNFYRNYDGSFMRISLEPTVGKYFELFKVSNYSSLTLI
NRYYPDSEEDKIVSTFRFRHSVPQYFSLSYGGFKFKNFLEGLYTFSPKDYEVQIFDYQDELNEENNFQFTLVGNLYKNRK
LMDYFLNTGYNLLGSYTFPTDGKKIDKKLLPLYYNVSVYPVENVRFWQDAVYDFNLGVFARNVIGTDISWKNFSVGISNS
VYKNSEKKVTTDQIALNLKYSGDKFFSGISLNYDRLVDKETYRNVYVGVKGKCWSLVLDYKRNYYRNRDEYVDQVFLRFN
LFFKEEIEIPLRR
>Mature_652_residues
ALLILFFLLGLSFSLEVFSEKLEIKENEIIAEGDAEAYYKNYYIKAKKIKLLRDKDEVYAYEDVYIKNLKTGFEIRGSFA
YVNMKKNKGYFLNAKGKFREFNFEAKKIEQLDKERFKVYEATVTTCPLDDKELYLCIFRANITKERALIFHNRLKFFKVP
IFYLPVYLIPLGGRKTGFLMPTIGSSTYSSFIYIQPFFWAISRDKDMTITADYRKDQMKGLSLEYRQAFDEESDLITQFS
LYKEDKKKGDWWKGREMYRENRFRIFMKYRKKPFKIGVEELSDPYYYEDISFKQEEITKPYTKTYLIYEKETKNYLFYFQ
TVRYRDLTEEKNNAVNLLPEASFHLKPFYYKGFNFSLDSSFTNFYRNYDGSFMRISLEPTVGKYFELFKVSNYSSLTLIN
RYYPDSEEDKIVSTFRFRHSVPQYFSLSYGGFKFKNFLEGLYTFSPKDYEVQIFDYQDELNEENNFQFTLVGNLYKNRKL
MDYFLNTGYNLLGSYTFPTDGKKIDKKLLPLYYNVSVYPVENVRFWQDAVYDFNLGVFARNVIGTDISWKNFSVGISNSV
YKNSEKKVTTDQIALNLKYSGDKFFSGISLNYDRLVDKETYRNVYVGVKGKCWSLVLDYKRNYYRNRDEYVDQVFLRFNL
FFKEEIEIPLRR

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

None

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 78316; Mature: 78185

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLILFFLLGLSFSLEVFSEKLEIKENEIIAEGDAEAYYKNYYIKAKKIKLLRDKDEVY
CHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEECCCCCCEEEEEEEEEEEEEEEECCCCEE
AYEDVYIKNLKTGFEIRGSFAYVNMKKNKGYFLNAKGKFREFNFEAKKIEQLDKERFKVY
EEEEEEEECCCCCEEEEEEEEEEEEECCCCEEEECCCCCEECCCCHHHHHHHHHHHHEEE
EATVTTCPLDDKELYLCIFRANITKERALIFHNRLKFFKVPIFYLPVYLIPLGGRKTGFL
EEEEEECCCCCCCEEEEEEECCCCCCHHHHHCCCHHHEECHHHHEEEEEEECCCCCCEEE
MPTIGSSTYSSFIYIQPFFWAISRDKDMTITADYRKDQMKGLSLEYRQAFDEESDLITQF
EECCCCCCCCCEEEEEEEEEEEECCCCEEEEECCCHHHHCCCCCHHHHHCCCCHHHHHHH
SLYKEDKKKGDWWKGREMYRENRFRIFMKYRKKPFKIGVEELSDPYYYEDISFKQEEITK
HHHHHHCCCCCCCCCHHHHHCCCEEEEEECCCCCEEECHHHCCCCEEECCCCCCHHHCCC
PYTKTYLIYEKETKNYLFYFQTVRYRDLTEEKNNAVNLLPEASFHLKPFYYKGFNFSLDS
CCEEEEEEEEECCCCEEEEEEEEEECCCCCCCCCCEEEECCCCCEECEEEEECCCEEECC
SFTNFYRNYDGSFMRISLEPTVGKYFELFKVSNYSSLTLINRYYPDSEEDKIVSTFRFRH
HHHHHHHCCCCCEEEEEECCCCCHHEEEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHH
SVPQYFSLSYGGFKFKNFLEGLYTFSPKDYEVQIFDYQDELNEENNFQFTLVGNLYKNRK
CCCCCEEECCCCCHHHHHHHHHHCCCCCCEEEEEEECHHHCCCCCCEEEEEEECCCCCCH
LMDYFLNTGYNLLGSYTFPTDGKKIDKKLLPLYYNVSVYPVENVRFWQDAVYDFNLGVFA
HHHHHHHCCCHHEEEECCCCCCHHHHHCCCCEEEEEEEEEECCCEEHHHHHEEECCCCEE
RNVIGTDISWKNFSVGISNSVYKNSEKKVTTDQIALNLKYSGDKFFSGISLNYDRLVDKE
HHHHCCCCCCCEEEEECCCCHHCCCCCCEEEEEEEEEEEECCCCEEEEECCCHHHHCCHH
TYRNVYVGVKGKCWSLVLDYKRNYYRNRDEYVDQVFLRFNLFFKEEIEIPLRR
HHCEEEEEECCCCEEHEEHHHHHHCCCHHHHHHHHHHHHEEEEEEECCCCCCC
>Mature Secondary Structure 
ALLILFFLLGLSFSLEVFSEKLEIKENEIIAEGDAEAYYKNYYIKAKKIKLLRDKDEVY
HHHHHHHHHCCCCCHHHHHHHHCCCCCEEEECCCCCCEEEEEEEEEEEEEEEECCCCEE
AYEDVYIKNLKTGFEIRGSFAYVNMKKNKGYFLNAKGKFREFNFEAKKIEQLDKERFKVY
EEEEEEEECCCCCEEEEEEEEEEEEECCCCEEEECCCCCEECCCCHHHHHHHHHHHHEEE
EATVTTCPLDDKELYLCIFRANITKERALIFHNRLKFFKVPIFYLPVYLIPLGGRKTGFL
EEEEEECCCCCCCEEEEEEECCCCCCHHHHHCCCHHHEECHHHHEEEEEEECCCCCCEEE
MPTIGSSTYSSFIYIQPFFWAISRDKDMTITADYRKDQMKGLSLEYRQAFDEESDLITQF
EECCCCCCCCCEEEEEEEEEEEECCCCEEEEECCCHHHHCCCCCHHHHHCCCCHHHHHHH
SLYKEDKKKGDWWKGREMYRENRFRIFMKYRKKPFKIGVEELSDPYYYEDISFKQEEITK
HHHHHHCCCCCCCCCHHHHHCCCEEEEEECCCCCEEECHHHCCCCEEECCCCCCHHHCCC
PYTKTYLIYEKETKNYLFYFQTVRYRDLTEEKNNAVNLLPEASFHLKPFYYKGFNFSLDS
CCEEEEEEEEECCCCEEEEEEEEEECCCCCCCCCCEEEECCCCCEECEEEEECCCEEECC
SFTNFYRNYDGSFMRISLEPTVGKYFELFKVSNYSSLTLINRYYPDSEEDKIVSTFRFRH
HHHHHHHCCCCCEEEEEECCCCCHHEEEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHH
SVPQYFSLSYGGFKFKNFLEGLYTFSPKDYEVQIFDYQDELNEENNFQFTLVGNLYKNRK
CCCCCEEECCCCCHHHHHHHHHHCCCCCCEEEEEEECHHHCCCCCCEEEEEEECCCCCCH
LMDYFLNTGYNLLGSYTFPTDGKKIDKKLLPLYYNVSVYPVENVRFWQDAVYDFNLGVFA
HHHHHHHCCCHHEEEECCCCCCHHHHHCCCCEEEEEEEEEECCCEEHHHHHEEECCCCEE
RNVIGTDISWKNFSVGISNSVYKNSEKKVTTDQIALNLKYSGDKFFSGISLNYDRLVDKE
HHHHCCCCCCCEEEEECCCCHHCCCCCCEEEEEEEEEEEECCCCEEEEECCCHHHHCCHH
TYRNVYVGVKGKCWSLVLDYKRNYYRNRDEYVDQVFLRFNLFFKEEIEIPLRR
HHCEEEEEECCCCEEHEEHHHHHHCCCHHHHHHHHHHHHEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA