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The map label for this gene is ispF

Identifier: 15606273

GI number: 15606273

Start: 657836

End: 658306

Strand: Direct

Name: ispF

Synonym: aq_957

Alternate gene names: 15606273

Gene position: 657836-658306 (Clockwise)

Preceding gene: 15606272

Following gene: 15606274

Centisome position: 42.4

GC content: 44.16

Gene sequence:

>471_bases
ATGGAACTCCGAATTGGTTTTGGTTTTGACTCTCACGAATTTGTAGAAGGAAAGTTATTAATTCTAGGCGGAGTGGAAAT
TGAAAAAGATTATGGACTGAAGGGGCACTCGGACGGGGACGCCCTTCTCCACGCTATAACGGACGCCATTCTCGGAGCCC
TCGGAGAGAGGGACATAGGTGAGATTTTTAAGGACACGGATCCGAGATGGAAAAACGCACCTTCGCGGATTTTCCTTGAA
AAGGCTCTGGAAGTAATGAGTGAAAAAGGATTTAACATATCAAATATCGACTGCGTAATTGTTGCAGACAGACCGAAGAT
AGCACCTCACAAGGAAAGGATAAAGGAAAGTCTTTCAAAACTCCTCGGCATTCCGAAGGAAAGGATTTCCTTAAAAGGAA
AGAGGAGAGAGGGTTTTTGTGAAGGAAACGGACTCGTGTGTATGTGTACGGTGTTGCTCGTTAAAATGTAA

Upstream 100 bases:

>100_bases
CTCCCGAAGAGGCTAATCCCCTTACGGCGGTAAGACAGATAATTTACGCTCTGCTGAATCCGAGACCTTCAAGAATAGGT
AAGCCTTTGATAGACAAGTG

Downstream 100 bases:

>100_bases
ATACCCATGGAAAATTTCCTCAGCTTCTTCAGGATACTGATAACCTTTCCGGTAGTCTTTCTTATACTGGAGAAAGAATT
TTTCCTCGCCTTCTGGCTTT

Product: hypothetical protein

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MELRIGFGFDSHEFVEGKLLILGGVEIEKDYGLKGHSDGDALLHAITDAILGALGERDIGEIFKDTDPRWKNAPSRIFLE
KALEVMSEKGFNISNIDCVIVADRPKIAPHKERIKESLSKLLGIPKERISLKGKRREGFCEGNGLVCMCTVLLVKM

Sequences:

>Translated_156_residues
MELRIGFGFDSHEFVEGKLLILGGVEIEKDYGLKGHSDGDALLHAITDAILGALGERDIGEIFKDTDPRWKNAPSRIFLE
KALEVMSEKGFNISNIDCVIVADRPKIAPHKERIKESLSKLLGIPKERISLKGKRREGFCEGNGLVCMCTVLLVKM
>Mature_156_residues
MELRIGFGFDSHEFVEGKLLILGGVEIEKDYGLKGHSDGDALLHAITDAILGALGERDIGEIFKDTDPRWKNAPSRIFLE
KALEVMSEKGFNISNIDCVIVADRPKIAPHKERIKESLSKLLGIPKERISLKGKRREGFCEGNGLVCMCTVLLVKM

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=41.6666666666667, Blast_Score=115, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_AQUAE (O67089)

Other databases:

- EMBL:   AE000657
- PIR:   F70382
- RefSeq:   NP_213652.1
- ProteinModelPortal:   O67089
- SMR:   O67089
- GeneID:   1193631
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_957
- NMPDR:   fig|224324.1.peg.667
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- PhylomeDB:   O67089
- ProtClustDB:   CLSK2299564
- BioCyc:   AAEO224324:AQ_957-MONOMER
- BRENDA:   4.6.1.12
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17307; Mature: 17307

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRIGFGFDSHEFVEGKLLILGGVEIEKDYGLKGHSDGDALLHAITDAILGALGERDIG
CEEEEECCCCCCCEECCEEEEEECEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
EIFKDTDPRWKNAPSRIFLEKALEVMSEKGFNISNIDCVIVADRPKIAPHKERIKESLSK
HHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
LLGIPKERISLKGKRREGFCEGNGLVCMCTVLLVKM
HHCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MELRIGFGFDSHEFVEGKLLILGGVEIEKDYGLKGHSDGDALLHAITDAILGALGERDIG
CEEEEECCCCCCCEECCEEEEEECEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
EIFKDTDPRWKNAPSRIFLEKALEVMSEKGFNISNIDCVIVADRPKIAPHKERIKESLSK
HHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
LLGIPKERISLKGKRREGFCEGNGLVCMCTVLLVKM
HHCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320