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The map label for this gene is lepB

Identifier: 15606272

GI number: 15606272

Start: 657066

End: 657836

Strand: Direct

Name: lepB

Synonym: aq_955

Alternate gene names: 15606272

Gene position: 657066-657836 (Clockwise)

Preceding gene: 15606271

Following gene: 15606273

Centisome position: 42.35

GC content: 44.88

Gene sequence:

>771_bases
ATGAGAAAGCAGATCGTAGAACTATTTTTAATAATCCTCGCTGTTTTATTCATAAGGGAGTACATAGCACAGGCTTACAC
CATTCCCTCCGCATCTATGGAGCCTACCCTTCTCGTAGGAGATTTCATACTGGTGAACAAACTCGTTTACTCGCTCTCTG
AACCCATGCGGGGGGATATGATAGTATTCAAGTATCCAAAGAATCCCGACATAGACTTCATAAAACGCATAATTGCAAGG
GGCGGGGATACCGTTGAGTTCTTTCCTTACTACGATGAGAAAAACAACGTCCTTATATACAAGGTTGCGGTAAACGGAAA
GCTTTACGAACTCACCTACGAAGGGGAAAAGAACTATTCCTACGACTGCTACCAGTACAGAGAAAAGCTCTACAGGGAAG
ACGGAGAAGTTATACAGCACAGCGTGTGTTTCAGGAATACACTTTTAAAAGTACCAGGGATGGTCTATAACGCGATAAGT
TCTGATCTCTGTCTGAAGTACAACGAAGACGGCTTTTGCGTTAAGTTTGTAGTGCCCGAAGGCTACTACTTCGTAATGGG
GGACAACAGGGACAACAGTCAGGACAGCAGGTTCTGGGGTTTTGTGCCGAGGGAAAACATAGAGGGTAAAGCTTTCGTGA
TTTACTACTCCGGAAAGGTCCCGTCTCTCACTCCCGAAGAGGCTAATCCCCTTACGGCGGTAAGACAGATAATTTACGCT
CTGCTGAATCCGAGACCTTCAAGAATAGGTAAGCCTTTGATAGACAAGTGA

Upstream 100 bases:

>100_bases
GTGTTGAGAGACTCGCGATGCTCCTCTTTGGCATAGATAACATAAAGCTCTTTTACGAAAACGACCTCAGGTTCATAAAG
CAGTTCTTCTGAAGGTTAAA

Downstream 100 bases:

>100_bases
TGGAACTCCGAATTGGTTTTGGTTTTGACTCTCACGAATTTGTAGAAGGAAAGTTATTAATTCTAGGCGGAGTGGAAATT
GAAAAAGATTATGGACTGAA

Product: type-I signal peptidase

Products: NA

Alternate protein names: SPase I; Leader peptidase I

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MRKQIVELFLIILAVLFIREYIAQAYTIPSASMEPTLLVGDFILVNKLVYSLSEPMRGDMIVFKYPKNPDIDFIKRIIAR
GGDTVEFFPYYDEKNNVLIYKVAVNGKLYELTYEGEKNYSYDCYQYREKLYREDGEVIQHSVCFRNTLLKVPGMVYNAIS
SDLCLKYNEDGFCVKFVVPEGYYFVMGDNRDNSQDSRFWGFVPRENIEGKAFVIYYSGKVPSLTPEEANPLTAVRQIIYA
LLNPRPSRIGKPLIDK

Sequences:

>Translated_256_residues
MRKQIVELFLIILAVLFIREYIAQAYTIPSASMEPTLLVGDFILVNKLVYSLSEPMRGDMIVFKYPKNPDIDFIKRIIAR
GGDTVEFFPYYDEKNNVLIYKVAVNGKLYELTYEGEKNYSYDCYQYREKLYREDGEVIQHSVCFRNTLLKVPGMVYNAIS
SDLCLKYNEDGFCVKFVVPEGYYFVMGDNRDNSQDSRFWGFVPRENIEGKAFVIYYSGKVPSLTPEEANPLTAVRQIIYA
LLNPRPSRIGKPLIDK
>Mature_256_residues
MRKQIVELFLIILAVLFIREYIAQAYTIPSASMEPTLLVGDFILVNKLVYSLSEPMRGDMIVFKYPKNPDIDFIKRIIAR
GGDTVEFFPYYDEKNNVLIYKVAVNGKLYELTYEGEKNYSYDCYQYREKLYREDGEVIQHSVCFRNTLLKVPGMVYNAIS
SDLCLKYNEDGFCVKFVVPEGYYFVMGDNRDNSQDSRFWGFVPRENIEGKAFVIYYSGKVPSLTPEEANPLTAVRQIIYA
LLNPRPSRIGKPLIDK

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family

Homologues:

Organism=Escherichia coli, GI1788921, Length=247, Percent_Identity=33.6032388663968, Blast_Score=126, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEP_AQUAE (O67088)

Other databases:

- EMBL:   AE000657
- PIR:   E70382
- RefSeq:   NP_213651.1
- ProteinModelPortal:   O67088
- SMR:   O67088
- MEROPS:   S26.001
- GeneID:   1193630
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_955
- NMPDR:   fig|224324.1.peg.666
- HOGENOM:   HBG596607
- OMA:   KIVPIAN
- PhylomeDB:   O67088
- ProtClustDB:   CLSK2299563
- BioCyc:   AAEO224324:AQ_955-MONOMER
- BRENDA:   3.4.21.89
- GO:   GO:0006508
- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019757
- InterPro:   IPR019756
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR019533
- Gene3D:   G3DSA:2.10.109.10
- PRINTS:   PR00727
- TIGRFAMs:   TIGR02227

Pfam domain/function: PF00717 Peptidase_S24; PF10502 Peptidase_S26; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.89

Molecular weight: Translated: 29677; Mature: 29677

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00501 SPASE_I_1; PS00760 SPASE_I_2; PS00761 SPASE_I_3

Important sites: ACT_SITE 32-32 ACT_SITE 75-75

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKQIVELFLIILAVLFIREYIAQAYTIPSASMEPTLLVGDFILVNKLVYSLSEPMRGDM
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCE
IVFKYPKNPDIDFIKRIIARGGDTVEFFPYYDEKNNVLIYKVAVNGKLYELTYEGEKNYS
EEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEEEECCEEEEEEECCCCCCC
YDCYQYREKLYREDGEVIQHSVCFRNTLLKVPGMVYNAISSDLCLKYNEDGFCVKFVVPE
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEECCCCEEEEEEECC
GYYFVMGDNRDNSQDSRFWGFVPRENIEGKAFVIYYSGKVPSLTPEEANPLTAVRQIIYA
CEEEEEECCCCCCCCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
LLNPRPSRIGKPLIDK
HHCCCHHHCCCCCCCC
>Mature Secondary Structure
MRKQIVELFLIILAVLFIREYIAQAYTIPSASMEPTLLVGDFILVNKLVYSLSEPMRGDM
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCE
IVFKYPKNPDIDFIKRIIARGGDTVEFFPYYDEKNNVLIYKVAVNGKLYELTYEGEKNYS
EEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEEEECCEEEEEEECCCCCCC
YDCYQYREKLYREDGEVIQHSVCFRNTLLKVPGMVYNAISSDLCLKYNEDGFCVKFVVPE
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEECCCCEEEEEEECC
GYYFVMGDNRDNSQDSRFWGFVPRENIEGKAFVIYYSGKVPSLTPEEANPLTAVRQIIYA
CEEEEEECCCCCCCCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
LLNPRPSRIGKPLIDK
HHCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9537320