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The map label for this gene is pheS

Identifier: 15606271

GI number: 15606271

Start: 656041

End: 657057

Strand: Direct

Name: pheS

Synonym: aq_953

Alternate gene names: 15606271

Gene position: 656041-657057 (Clockwise)

Preceding gene: 15606270

Following gene: 15606272

Centisome position: 42.29

GC content: 45.62

Gene sequence:

>1017_bases
ATGGAAAAGCTCGACAAAATCCTCGAGGAGTTAAAATTACTCCTCTCTTCTGTCTCTTCCCTTAAAGAACTTCAAGAAGT
AAGGAGCAAGTTCTTAGGGAGTAAGGGTGTCATAAAGGAGCTTCTCAAGAAAATCAAGGAAGTTCCCTCCGAAGAGAGGA
AGGAGTACGGAAAAAGAGTTAACCTACTCAAGGAAGAGGCTGAAAAGCTAATAAAGGAAAAGGAAGAGGAACTCAAGGAA
AGGGAACTTGAAGAAAAGCTAAAAGGCGAATGGGTTGACCTGAGCATTCCTCCGGCTAGGACTGTAGGTTCTCTTCACCC
CATAACAGTTACGCTCGAGAGGATTGTGACGATATTCAGAGGAATGGGATTTGAGGTTGAGGAAGGTCCCGAGGTAGAGA
GGGAAGAGTATAACTTTGATATGCTCAACATTCCCAAGGAACACCCTGCAAGGGACATGCAGGATACTTTTTACGTAAAC
AGGGAGGGCTACCTTCTGAGGACTCACACCTCTCCCGTCCAGATAAGAACCATGCTCAAGAAAAAGCCTCCCATCCAGAT
AATAGCCCCCGGGAAGGTTTACAGGAGGGACGACGATCCCACTCACTCTCCTATGTTCCATCAGGTTGAAGGGCTTGTTG
TAAACGAGTATGCAAACTTCAGACACATGAAGTACGTAATTGAGGAATTCCTGAAGAAGTTCTTTGAAACTGACCTTCCT
GTGAGGTTCAGAACCTCATACTTTCCCTTCACGGAGCCCTCCGCGGAAGTTGACATAGGTTGTGTTATATGTCATCAGGA
GGGTTGCAGGGTTTGTAAACATACGGGATGGCTTGAAGTAATGGGTTGCGGAATGGTTCACCCTAAGGTTCTCGAAAACT
GCGGTATAGACACGGATTTTTACCAGGGATTTGCCTTCGGAATGGGTGTTGAGAGACTCGCGATGCTCCTCTTTGGCATA
GATAACATAAAGCTCTTTTACGAAAACGACCTCAGGTTCATAAAGCAGTTCTTCTGA

Upstream 100 bases:

>100_bases
TAGAACTAGACAGGAAGATCCTCGCGGATATGGCGGTAAGAGATCCCCAAGCTTTTGAACAGGTCGTTAACAAAGTTAAG
GAAGCCCTGCAAGTTCAGTG

Downstream 100 bases:

>100_bases
AGGTTAAAATGAGAAAGCAGATCGTAGAACTATTTTTAATAATCCTCGCTGTTTTATTCATAAGGGAGTACATAGCACAG
GCTTACACCATTCCCTCCGC

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MEKLDKILEELKLLLSSVSSLKELQEVRSKFLGSKGVIKELLKKIKEVPSEERKEYGKRVNLLKEEAEKLIKEKEEELKE
RELEEKLKGEWVDLSIPPARTVGSLHPITVTLERIVTIFRGMGFEVEEGPEVEREEYNFDMLNIPKEHPARDMQDTFYVN
REGYLLRTHTSPVQIRTMLKKKPPIQIIAPGKVYRRDDDPTHSPMFHQVEGLVVNEYANFRHMKYVIEEFLKKFFETDLP
VRFRTSYFPFTEPSAEVDIGCVICHQEGCRVCKHTGWLEVMGCGMVHPKVLENCGIDTDFYQGFAFGMGVERLAMLLFGI
DNIKLFYENDLRFIKQFF

Sequences:

>Translated_338_residues
MEKLDKILEELKLLLSSVSSLKELQEVRSKFLGSKGVIKELLKKIKEVPSEERKEYGKRVNLLKEEAEKLIKEKEEELKE
RELEEKLKGEWVDLSIPPARTVGSLHPITVTLERIVTIFRGMGFEVEEGPEVEREEYNFDMLNIPKEHPARDMQDTFYVN
REGYLLRTHTSPVQIRTMLKKKPPIQIIAPGKVYRRDDDPTHSPMFHQVEGLVVNEYANFRHMKYVIEEFLKKFFETDLP
VRFRTSYFPFTEPSAEVDIGCVICHQEGCRVCKHTGWLEVMGCGMVHPKVLENCGIDTDFYQGFAFGMGVERLAMLLFGI
DNIKLFYENDLRFIKQFF
>Mature_338_residues
MEKLDKILEELKLLLSSVSSLKELQEVRSKFLGSKGVIKELLKKIKEVPSEERKEYGKRVNLLKEEAEKLIKEKEEELKE
RELEEKLKGEWVDLSIPPARTVGSLHPITVTLERIVTIFRGMGFEVEEGPEVEREEYNFDMLNIPKEHPARDMQDTFYVN
REGYLLRTHTSPVQIRTMLKKKPPIQIIAPGKVYRRDDDPTHSPMFHQVEGLVVNEYANFRHMKYVIEEFLKKFFETDLP
VRFRTSYFPFTEPSAEVDIGCVICHQEGCRVCKHTGWLEVMGCGMVHPKVLENCGIDTDFYQGFAFGMGVERLAMLLFGI
DNIKLFYENDLRFIKQFF

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=236, Percent_Identity=32.2033898305085, Blast_Score=121, Evalue=7e-28,
Organism=Homo sapiens, GI4758340, Length=307, Percent_Identity=30.9446254071661, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1788007, Length=338, Percent_Identity=48.2248520710059, Blast_Score=337, Evalue=9e-94,
Organism=Caenorhabditis elegans, GI32566635, Length=253, Percent_Identity=33.596837944664, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17508957, Length=289, Percent_Identity=29.4117647058824, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI32563657, Length=289, Percent_Identity=29.4117647058824, Blast_Score=118, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6321087, Length=268, Percent_Identity=30.2238805970149, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6325304, Length=280, Percent_Identity=29.2857142857143, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17137424, Length=294, Percent_Identity=28.9115646258503, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI18858079, Length=348, Percent_Identity=28.735632183908, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_AQUAE (O67087)

Other databases:

- EMBL:   AE000657
- PIR:   D70382
- RefSeq:   NP_213650.1
- ProteinModelPortal:   O67087
- SMR:   O67087
- GeneID:   1193629
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_953
- NMPDR:   fig|224324.1.peg.665
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- PhylomeDB:   O67087
- ProtClustDB:   PRK00488
- BioCyc:   AAEO224324:AQ_953-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 39504; Mature: 39504

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLDKILEELKLLLSSVSSLKELQEVRSKFLGSKGVIKELLKKIKEVPSEERKEYGKRV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
NLLKEEAEKLIKEKEEELKERELEEKLKGEWVDLSIPPARTVGSLHPITVTLERIVTIFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHCCCCCHHHHHHHHHHHHHH
GMGFEVEEGPEVEREEYNFDMLNIPKEHPARDMQDTFYVNREGYLLRTHTSPVQIRTMLK
CCCCCCCCCCCCCHHHCCCCEECCCCCCCCCCCCHHEEECCCCEEEEECCCHHHHHHHHH
KKPPIQIIAPGKVYRRDDDPTHSPMFHQVEGLVVNEYANFRHMKYVIEEFLKKFFETDLP
CCCCEEEECCCCCEECCCCCCCCCHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCC
VRFRTSYFPFTEPSAEVDIGCVICHQEGCRVCKHTGWLEVMGCGMVHPKVLENCGIDTDF
EEEECCCCCCCCCCCCCEEEEEEECCHHHHHHHHCCCHHHHCCCCCCHHHHHHCCCCHHH
YQGFAFGMGVERLAMLLFGIDNIKLFYENDLRFIKQFF
HHHHHHHCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHC
>Mature Secondary Structure
MEKLDKILEELKLLLSSVSSLKELQEVRSKFLGSKGVIKELLKKIKEVPSEERKEYGKRV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
NLLKEEAEKLIKEKEEELKERELEEKLKGEWVDLSIPPARTVGSLHPITVTLERIVTIFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHCCCCCHHHHHHHHHHHHHH
GMGFEVEEGPEVEREEYNFDMLNIPKEHPARDMQDTFYVNREGYLLRTHTSPVQIRTMLK
CCCCCCCCCCCCCHHHCCCCEECCCCCCCCCCCCHHEEECCCCEEEEECCCHHHHHHHHH
KKPPIQIIAPGKVYRRDDDPTHSPMFHQVEGLVVNEYANFRHMKYVIEEFLKKFFETDLP
CCCCEEEECCCCCEECCCCCCCCCHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCC
VRFRTSYFPFTEPSAEVDIGCVICHQEGCRVCKHTGWLEVMGCGMVHPKVLENCGIDTDF
EEEECCCCCCCCCCCCCEEEEEEECCHHHHHHHHCCCHHHHCCCCCCHHHHHHCCCCHHH
YQGFAFGMGVERLAMLLFGIDNIKLFYENDLRFIKQFF
HHHHHHHCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320