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The map label for this gene is rho

Identifier: 15606215

GI number: 15606215

Start: 603214

End: 604524

Strand: Direct

Name: rho

Synonym: aq_873

Alternate gene names: 15606215

Gene position: 603214-604524 (Clockwise)

Preceding gene: 15606214

Following gene: 15607141

Centisome position: 38.88

GC content: 46.68

Gene sequence:

>1311_bases
ATGACTCAGCAAACCGCTCAACAAGAACAACAAAAGAGTGAAGTAAAAATTTACAGCAGGGAAGAGTTAAAACAAAAGAC
CTTAGCGGAGCTCCAGAAAATCGGGAAGGAGCTCGGACTCACCAGAACCACGGGACTTAAAAAAGAGGAGTTAATAGAGA
AAATACTAAAAGCTCAGCTCGAAAAGAGCAGACTCGTTTTCGTAAAAGGTGTCCTCGAAATTCTCCCCGAAGGCTACGGC
TTCTTAAGGATGCCGGAGAACAACTACATGCCAAGCTGGAACGACGTTTACGTTGCCCCCTCCCAGATAAAGAAGTTCGG
ACTCAGAACGGGAGATACGATTGAGGGCTTTGCGAGACTGCCCAGAGAAAACGAAAAGTACAAGGCGTTGATCAGAATGG
AATCCGTGAACGGACTTCCGCCTGACCCCGAAATACTCAAGAGAAGACCCCAGTTTGAAAAGCTCACACCCCTTCACCCC
ATGGAAAGGTTCAAACTCGAGTACGACCCGAATGAGCTCTCCACCCGTGTGGTCAGCCTGATAGCACCTATAGGTAAGGG
ACAGAGAGGATTAATAGTAGCACCTCCGAAGGCCGGAAAAACGGTTCTCCTCCAGAAAATAGCCCAAGCTATTATAAGAA
ACCACCCAGAAGTTTACCTCATAATTCTCCTGATAGACGAAAGACCCGAAGAAGTCACTGAAATGAGAAGGATAGTGAAG
GACAAGGCTGAAGTAGTAGCATCTACTTTTGACGAACCACCCGAAAGGCACATGCAGGTTGCGGAAATAGTCGTGGAAAA
GGCAAAGAGAATGGTAGAACTCAAGAAAGACGTAGTAATACTTATGGACTCCTTGACAAGGTTTACGAGAGCTTCTAACG
CGGTGACTCCTCCTACGGGCAGAGTTCTCACGGGTGGTATAGAAATCACAGCCTTCCAGAGACCTAAAAAGTTCTTTGGT
GCTGCGCGGAACATAGAAGAGGGAGGAAGCCTCACCATAATCGCAACCGCTCTCGTAGAGACAGGTTCAAAGATGGACGA
TGTAATCTACGAAGAGTTCAAAGGAACGGGTAATATGGAAATACACCTCGACAGAAGACTAATGGAAAGGAGGATATTCC
CCGCCATAAACATAGAAAAGTCAGGAACCAGAAAGGAAGAACTCCTTTTAGAGCCCTGGGAACTCCAGAGGATTTGGGTT
CTCAGGAAGTTCCTGTCAACAATGGACCCTGTGGAGGCTATGGAATTCCTCCTTGAAAAACTGAGAAGGTTCAAAACGAA
CGAGGAGTTCCTTAAAGCCATGAACGCTTGA

Upstream 100 bases:

>100_bases
GGACTACTTGGGAGAAATTTTGGGAGAGATTACCACGGAGGATCTTTTAGGAAAAATCTTTTCCACCTTCTGCATAGGAA
AGTAAGAAGAGGAGGTTATC

Downstream 100 bases:

>100_bases
AAGCGAAGAACTTTTATAATAACTTTTTTATCTTGCGAGGAGGTAGGTAAATGAAGAAGGGTATTCACCCCGAACTAAAA
CCCACAACTTTCGTATGCGG

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MTQQTAQQEQQKSEVKIYSREELKQKTLAELQKIGKELGLTRTTGLKKEELIEKILKAQLEKSRLVFVKGVLEILPEGYG
FLRMPENNYMPSWNDVYVAPSQIKKFGLRTGDTIEGFARLPRENEKYKALIRMESVNGLPPDPEILKRRPQFEKLTPLHP
MERFKLEYDPNELSTRVVSLIAPIGKGQRGLIVAPPKAGKTVLLQKIAQAIIRNHPEVYLIILLIDERPEEVTEMRRIVK
DKAEVVASTFDEPPERHMQVAEIVVEKAKRMVELKKDVVILMDSLTRFTRASNAVTPPTGRVLTGGIEITAFQRPKKFFG
AARNIEEGGSLTIIATALVETGSKMDDVIYEEFKGTGNMEIHLDRRLMERRIFPAINIEKSGTRKEELLLEPWELQRIWV
LRKFLSTMDPVEAMEFLLEKLRRFKTNEEFLKAMNA

Sequences:

>Translated_436_residues
MTQQTAQQEQQKSEVKIYSREELKQKTLAELQKIGKELGLTRTTGLKKEELIEKILKAQLEKSRLVFVKGVLEILPEGYG
FLRMPENNYMPSWNDVYVAPSQIKKFGLRTGDTIEGFARLPRENEKYKALIRMESVNGLPPDPEILKRRPQFEKLTPLHP
MERFKLEYDPNELSTRVVSLIAPIGKGQRGLIVAPPKAGKTVLLQKIAQAIIRNHPEVYLIILLIDERPEEVTEMRRIVK
DKAEVVASTFDEPPERHMQVAEIVVEKAKRMVELKKDVVILMDSLTRFTRASNAVTPPTGRVLTGGIEITAFQRPKKFFG
AARNIEEGGSLTIIATALVETGSKMDDVIYEEFKGTGNMEIHLDRRLMERRIFPAINIEKSGTRKEELLLEPWELQRIWV
LRKFLSTMDPVEAMEFLLEKLRRFKTNEEFLKAMNA
>Mature_435_residues
TQQTAQQEQQKSEVKIYSREELKQKTLAELQKIGKELGLTRTTGLKKEELIEKILKAQLEKSRLVFVKGVLEILPEGYGF
LRMPENNYMPSWNDVYVAPSQIKKFGLRTGDTIEGFARLPRENEKYKALIRMESVNGLPPDPEILKRRPQFEKLTPLHPM
ERFKLEYDPNELSTRVVSLIAPIGKGQRGLIVAPPKAGKTVLLQKIAQAIIRNHPEVYLIILLIDERPEEVTEMRRIVKD
KAEVVASTFDEPPERHMQVAEIVVEKAKRMVELKKDVVILMDSLTRFTRASNAVTPPTGRVLTGGIEITAFQRPKKFFGA
ARNIEEGGSLTIIATALVETGSKMDDVIYEEFKGTGNMEIHLDRRLMERRIFPAINIEKSGTRKEELLLEPWELQRIWVL
RKFLSTMDPVEAMEFLLEKLRRFKTNEEFLKAMNA

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family

Homologues:

Organism=Escherichia coli, GI1790217, Length=416, Percent_Identity=56.9711538461538, Blast_Score=485, Evalue=1e-138,
Organism=Escherichia coli, GI1788251, Length=263, Percent_Identity=28.1368821292776, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): RHO_AQUAE (O67031)

Other databases:

- EMBL:   AE000657
- PIR:   D70375
- RefSeq:   NP_213592.1
- ProteinModelPortal:   O67031
- SMR:   O67031
- GeneID:   1193573
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_873
- NMPDR:   fig|224324.1.peg.607
- HOGENOM:   HBG335262
- OMA:   SPSQIKR
- PhylomeDB:   O67031
- ProtClustDB:   PRK09376
- BioCyc:   AAEO224324:AQ_873-MONOMER
- HAMAP:   MF_01884
- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00382
- SMART:   SM00357
- SMART:   SM00959
- TIGRFAMs:   TIGR00767

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind; SSF50249 Nucleic_acid_OB; SSF68912 Rho_N

EC number: NA

Molecular weight: Translated: 50092; Mature: 49961

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: BINDING 228-228

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQTAQQEQQKSEVKIYSREELKQKTLAELQKIGKELGLTRTTGLKKEELIEKILKAQL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
EKSRLVFVKGVLEILPEGYGFLRMPENNYMPSWNDVYVAPSQIKKFGLRTGDTIEGFARL
HHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCEEECHHHHHHHCCCCCCHHHHHHHC
PRENEKYKALIRMESVNGLPPDPEILKRRPQFEKLTPLHPMERFKLEYDPNELSTRVVSL
CCCCCHHHHHHEECCCCCCCCCHHHHHCCCCCCCCCCCCCHHHCEECCCCHHHHHHHHHH
IAPIGKGQRGLIVAPPKAGKTVLLQKIAQAIIRNHPEVYLIILLIDERPEEVTEMRRIVK
HHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHH
DKAEVVASTFDEPPERHMQVAEIVVEKAKRMVELKKDVVILMDSLTRFTRASNAVTPPTG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
RVLTGGIEITAFQRPKKFFGAARNIEEGGSLTIIATALVETGSKMDDVIYEEFKGTGNME
CEEECCEEEEEECCCHHHHHHHCCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHCCCCCEE
IHLDRRLMERRIFPAINIEKSGTRKEELLLEPWELQRIWVLRKFLSTMDPVEAMEFLLEK
EEEHHHHHHHHCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LRRFKTNEEFLKAMNA
HHHHCCCHHHHHHHCC
>Mature Secondary Structure 
TQQTAQQEQQKSEVKIYSREELKQKTLAELQKIGKELGLTRTTGLKKEELIEKILKAQL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
EKSRLVFVKGVLEILPEGYGFLRMPENNYMPSWNDVYVAPSQIKKFGLRTGDTIEGFARL
HHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCEEECHHHHHHHCCCCCCHHHHHHHC
PRENEKYKALIRMESVNGLPPDPEILKRRPQFEKLTPLHPMERFKLEYDPNELSTRVVSL
CCCCCHHHHHHEECCCCCCCCCHHHHHCCCCCCCCCCCCCHHHCEECCCCHHHHHHHHHH
IAPIGKGQRGLIVAPPKAGKTVLLQKIAQAIIRNHPEVYLIILLIDERPEEVTEMRRIVK
HHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHH
DKAEVVASTFDEPPERHMQVAEIVVEKAKRMVELKKDVVILMDSLTRFTRASNAVTPPTG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
RVLTGGIEITAFQRPKKFFGAARNIEEGGSLTIIATALVETGSKMDDVIYEEFKGTGNME
CEEECCEEEEEECCCHHHHHHHCCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHCCCCCEE
IHLDRRLMERRIFPAINIEKSGTRKEELLLEPWELQRIWVLRKFLSTMDPVEAMEFLLEK
EEEHHHHHHHHCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LRRFKTNEEFLKAMNA
HHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320