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The map label for this gene is trmE

Identifier: 15606214

GI number: 15606214

Start: 601852

End: 603198

Strand: Direct

Name: trmE

Synonym: aq_871

Alternate gene names: 15606214

Gene position: 601852-603198 (Clockwise)

Preceding gene: 15606207

Following gene: 15606215

Centisome position: 38.8

GC content: 44.69

Gene sequence:

>1347_bases
ATGAGAGAACCCATCGTAGCTATAGCCACTCCTTACGGTGAGAGTGCAATAGGTATAGTTCGCCTATCGGGAAAGGGCGT
ACTGGACTTAGTTAAGAAGTTCTTCAAAACGAAGAGGGAAATAAAGCCGAGGTACGCCCACTTCGGCGTTCTCTACGACG
ATAAAGGCGAAGAACTCGACGAAGGAGTCCTTATATACTACAAAGCTCCTCACTCCTATACGGGCGAGGATATGGTAGAA
CTCAACCTCCACGGAAATCCGAGGATTCTCAAAAGAGCTCTGGAACTTTTTGTAAACGCCGGAGCACGGCTCGCAGAACC
CGGAGAGTTTACCAAGAGAGCCTTTTTAAACGGAAAACTGGATTTAACTCAGGCGGAAGCCGTTGCGGAGTTAATATCCG
CAAAAACGGAACTTGCGAGGAAGGTAGCCCTGAAACAACTTCACGGAGAGCTTTCAAAACACATAAGACCTTTGAGAGAA
ACGCTTCTGGAACTCCTCGCCTACGTAGAGGCTGACATAGAGTTCGCGGAGGAAGACATACCGACCCTAACAAAGGAACA
GGTAATCCAGATGGTGGACAAAGTAATAGAGGGAATAAACGAACTCCTGAAAACCGCAAAAACCGGAAAGTTCATAAGGG
AAGGTGTAAAATTAGCTATCGTTGGAAGACCCAACGTAGGAAAGTCCTCCCTCTTTAACGCACTCCTCAAAGAGGAGAGG
GCTATAGTCACGGACATAGCTGGAACCACGAGGGACTTTATAGAGGAAACTCTCCAGATAAAGGGAGTGCCCGTAAGACT
CGTAGACACCGCGGGAATAAGGGAAACTAAAGACCTGGTTGAGAGAATAGGAGTAGAAAGGAGCAAACAAAAGGTTAAGG
AAGCGGATCTTATTCTCTTCGTAATAGACGCAAGTCAGGAAATTACCGAGGAGGATTTAAGGATATACGAGGAGATAAAG
GAAAAGGATCACATCGTAGTGGCTAACAAGGTGGACTTAGGGATTAGGGCAAATCTCGAAAAGTTTAAAGGAGAAAAAAT
TGTAAAGGTGAGTGCTTTAAAGGGAACTGGACTGGAGGAGCTTTCCGAGGAAATTCTGAAAAAAGTCGGTGCAAATCTCG
AAGAAAGTGTTAATATATACATATCCGTTAGACACGAAACTCTTCTCAAAAAGGCAAAAGAAGTTTTAGAAAGGTTTAAG
GAGGAGTTCAGGGAAAAAGATATAAGTCCTGAAATCGCCATGCTTGACCTGAGGGAAGCTTCGGACTACTTGGGAGAAAT
TTTGGGAGAGATTACCACGGAGGATCTTTTAGGAAAAATCTTTTCCACCTTCTGCATAGGAAAGTAA

Upstream 100 bases:

>100_bases
CGCCCAGGGGATTTTAAGTCCCCCGCGTCTGCCAATTCCGCCACACCCCCTAACTAAAGAAATATATTATACTTATAGAA
AGAACTTTTCTCAAGAAATC

Downstream 100 bases:

>100_bases
GAAGAGGAGGTTATCATGACTCAGCAAACCGCTCAACAAGAACAACAAAAGAGTGAAGTAAAAATTTACAGCAGGGAAGA
GTTAAAACAAAAGACCTTAG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MREPIVAIATPYGESAIGIVRLSGKGVLDLVKKFFKTKREIKPRYAHFGVLYDDKGEELDEGVLIYYKAPHSYTGEDMVE
LNLHGNPRILKRALELFVNAGARLAEPGEFTKRAFLNGKLDLTQAEAVAELISAKTELARKVALKQLHGELSKHIRPLRE
TLLELLAYVEADIEFAEEDIPTLTKEQVIQMVDKVIEGINELLKTAKTGKFIREGVKLAIVGRPNVGKSSLFNALLKEER
AIVTDIAGTTRDFIEETLQIKGVPVRLVDTAGIRETKDLVERIGVERSKQKVKEADLILFVIDASQEITEEDLRIYEEIK
EKDHIVVANKVDLGIRANLEKFKGEKIVKVSALKGTGLEELSEEILKKVGANLEESVNIYISVRHETLLKKAKEVLERFK
EEFREKDISPEIAMLDLREASDYLGEILGEITTEDLLGKIFSTFCIGK

Sequences:

>Translated_448_residues
MREPIVAIATPYGESAIGIVRLSGKGVLDLVKKFFKTKREIKPRYAHFGVLYDDKGEELDEGVLIYYKAPHSYTGEDMVE
LNLHGNPRILKRALELFVNAGARLAEPGEFTKRAFLNGKLDLTQAEAVAELISAKTELARKVALKQLHGELSKHIRPLRE
TLLELLAYVEADIEFAEEDIPTLTKEQVIQMVDKVIEGINELLKTAKTGKFIREGVKLAIVGRPNVGKSSLFNALLKEER
AIVTDIAGTTRDFIEETLQIKGVPVRLVDTAGIRETKDLVERIGVERSKQKVKEADLILFVIDASQEITEEDLRIYEEIK
EKDHIVVANKVDLGIRANLEKFKGEKIVKVSALKGTGLEELSEEILKKVGANLEESVNIYISVRHETLLKKAKEVLERFK
EEFREKDISPEIAMLDLREASDYLGEILGEITTEDLLGKIFSTFCIGK
>Mature_448_residues
MREPIVAIATPYGESAIGIVRLSGKGVLDLVKKFFKTKREIKPRYAHFGVLYDDKGEELDEGVLIYYKAPHSYTGEDMVE
LNLHGNPRILKRALELFVNAGARLAEPGEFTKRAFLNGKLDLTQAEAVAELISAKTELARKVALKQLHGELSKHIRPLRE
TLLELLAYVEADIEFAEEDIPTLTKEQVIQMVDKVIEGINELLKTAKTGKFIREGVKLAIVGRPNVGKSSLFNALLKEER
AIVTDIAGTTRDFIEETLQIKGVPVRLVDTAGIRETKDLVERIGVERSKQKVKEADLILFVIDASQEITEEDLRIYEEIK
EKDHIVVANKVDLGIRANLEKFKGEKIVKVSALKGTGLEELSEEILKKVGANLEESVNIYISVRHETLLKKAKEVLERFK
EEFREKDISPEIAMLDLREASDYLGEILGEITTEDLLGKIFSTFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=465, Percent_Identity=33.9784946236559, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI193082988, Length=494, Percent_Identity=31.7813765182186, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI193082993, Length=454, Percent_Identity=33.7004405286344, Blast_Score=226, Evalue=3e-59,
Organism=Escherichia coli, GI2367268, Length=451, Percent_Identity=40.3547671840355, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI87082120, Length=205, Percent_Identity=32.6829268292683, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17507259, Length=454, Percent_Identity=31.2775330396476, Blast_Score=197, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6323665, Length=495, Percent_Identity=32.7272727272727, Blast_Score=226, Evalue=4e-60,
Organism=Drosophila melanogaster, GI45550813, Length=480, Percent_Identity=33.75, Blast_Score=222, Evalue=5e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_AQUAE (O67030)

Other databases:

- EMBL:   AE000657
- PIR:   C70375
- RefSeq:   NP_213591.1
- ProteinModelPortal:   O67030
- SMR:   O67030
- DIP:   DIP-48312N
- GeneID:   1193572
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_871
- NMPDR:   fig|224324.1.peg.606
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- PhylomeDB:   O67030
- ProtClustDB:   PRK05291
- BioCyc:   AAEO224324:AQ_871-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 50305; Mature: 50305

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: BINDING 21-21 BINDING 80-80 BINDING 119-119 BINDING 448-448

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREPIVAIATPYGESAIGIVRLSGKGVLDLVKKFFKTKREIKPRYAHFGVLYDDKGEELD
CCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCC
EGVLIYYKAPHSYTGEDMVELNLHGNPRILKRALELFVNAGARLAEPGEFTKRAFLNGKL
CCEEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCC
DLTQAEAVAELISAKTELARKVALKQLHGELSKHIRPLRETLLELLAYVEADIEFAEEDI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PTLTKEQVIQMVDKVIEGINELLKTAKTGKFIREGVKLAIVGRPNVGKSSLFNALLKEER
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHH
AIVTDIAGTTRDFIEETLQIKGVPVRLVDTAGIRETKDLVERIGVERSKQKVKEADLILF
HHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCHHHHHHHHHCCEEEE
VIDASQEITEEDLRIYEEIKEKDHIVVANKVDLGIRANLEKFKGEKIVKVSALKGTGLEE
EECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHCCCCEEEEEECCCCCHHH
LSEEILKKVGANLEESVNIYISVRHETLLKKAKEVLERFKEEFREKDISPEIAMLDLREA
HHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHH
SDYLGEILGEITTEDLLGKIFSTFCIGK
HHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MREPIVAIATPYGESAIGIVRLSGKGVLDLVKKFFKTKREIKPRYAHFGVLYDDKGEELD
CCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCC
EGVLIYYKAPHSYTGEDMVELNLHGNPRILKRALELFVNAGARLAEPGEFTKRAFLNGKL
CCEEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCC
DLTQAEAVAELISAKTELARKVALKQLHGELSKHIRPLRETLLELLAYVEADIEFAEEDI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PTLTKEQVIQMVDKVIEGINELLKTAKTGKFIREGVKLAIVGRPNVGKSSLFNALLKEER
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHH
AIVTDIAGTTRDFIEETLQIKGVPVRLVDTAGIRETKDLVERIGVERSKQKVKEADLILF
HHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCHHHHHHHHHCCEEEE
VIDASQEITEEDLRIYEEIKEKDHIVVANKVDLGIRANLEKFKGEKIVKVSALKGTGLEE
EECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHCCCCEEEEEECCCCCHHH
LSEEILKKVGANLEESVNIYISVRHETLLKKAKEVLERFKEEFREKDISPEIAMLDLREA
HHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHH
SDYLGEILGEITTEDLLGKIFSTFCIGK
HHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320