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The map label for this gene is gidA1

Identifier: 15606146

GI number: 15606146

Start: 526457

End: 528310

Strand: Direct

Name: gidA1

Synonym: aq_761

Alternate gene names: 15606146

Gene position: 526457-528310 (Clockwise)

Preceding gene: 15606135

Following gene: 15606147

Centisome position: 33.94

GC content: 48.49

Gene sequence:

>1854_bases
ATGGCTTGGGTAGTTGACGAGTTTGACGTGGTAGTTATAGGAGGCGGACACGCTGGGATAGAGGCAGCCCTTGCGGCAGC
GAGAATGGGCGCAAAGACAGCGATGTTCGTTCTGAACGCGGATACCATAGGACAGATGTCCTGCAACCCCGCAATAGGAG
GAATAGCAAAGGGAATAGTGGTAAGGGAGATAGACGCCCTCGGGGGAGAGATGGGAAAGGCTATAGACCAGACGGGTATT
CAGTTCAAGATGCTGAATACGAGAAAGGGAAAAGCAGTCCAGTCTCCGAGAGCTCAGGCGGACAAAAAACGCTACAGGGA
GTACATGAAAAAGGTATGTGAAAATCAGGAAAACCTTTACATAAAACAGGAAGAAGTTGTGGACATAATAGTGAAGAACA
ATCAAGTTGTGGGTGTAAGGACGAACCTCGGAGTTGAGTACAAAACGAAAGCGGTTGTCGTGACTACGGGAACCTTTCTG
AACGGCGTTATATACATAGGCGACAAGATGATACCCGGGGGAAGGCTCGGAGAACCGAGAAGCGAAGGGCTTTCCGACTT
TTACAGGAGATTTGATTTTCCCCTAATACGCTTTAAGACTGGAACGCCCGCGAGACTTGACAAGAGAACCATAGACTTTT
CCGCTCTAGAAGTAGCTCCCGGAGACGACCCGCCCCCTAAGTTTTCCTTCTGGACCGAGCCCGTGGGCTCTTACTGGTTC
CCTAAAGGAAAAGAACAGGTAAACTGCTGGATTACGTATACGACTCCCAAAACCCACGAAATTATCAGGAAGAACCTCCA
CAGAACGGCCCTTTACGGCGGTCTCATTAAGGGAATAGGACCAAGGTACTGCCCCTCAATTGAGGACAAGATAGTGAAGT
TCCCCGACAAGGAGAGACACCAGATATTCCTAGAGCCCGAAGGTCTGGACACAATAGAAATATACCCGAACGGACTCTCC
ACTTCTTTACCCGAAGAGGTCCAGTGGGAGATGTACCGCTCAATCCCCGGACTTGAAAACGTAGTCCTAATCAGACCCGC
TTACGCCATAGAGTACGACGTTGTTCCGCCCACAGAACTTTACCCAACTCTTGAGACGAAAAAGATAAGGGGTCTCTTCC
ACGCGGGTAATTTCAACGGAACTACGGGATACGAGGAAGCAGCAGGTCAAGGAATAGTGGCGGGAATAAACGCAGCCCTC
AGAGCTTTCGGAAAAGAGCCCATATACCTGAGAAGGGACGAGAGCTACATAGGCGTTATGATAGATGACCTCACGACTAA
AGGAGTTACGGAACCCTACAGACTCTTTACCTCCCGTTCTGAATACAGGCTGTATATAAGGCAGGATAACGCAATACTTA
GACTAGCAAAGCTCGGAAGGGAACTCGGACTCCTCAGTGAGGAGCAGTATAAACTCGTAAAAGAACTCGAAAGGGAAATA
GAGAAGTGGAAGGAGTTTTACAAGAGCGAAAGGGTAAGCGTTGCGGTGGGAGGAGACACGAGGAGTTACAGCGTTGCCAC
ACTAATGACCATGAACTACACCCTTGACGATGTAAAAGAGAAGTTCGGATACGAAGTGCCCCAGCACCCTTACGTAAAAG
AAGAAGTGGAAATCCAGCTAAAGTACGAGCCCTACATAGAGAGGGAGAGGAAATTAAACGAAAAGTTAAAGAAACTTGAA
GACACGAAAATACCACCCGACATTGATTACGACAAAATCCCGGGTCTTACGAAAGAGGCGAGGGAAAAACTTAAGAAATT
CAAACCCATAACCGTAGGTCAGGCCTCCAGAATTGACGGAATTACGCCCGCAGCGATAACGGCACTACTCGTTTACCTCG
GAAAGTTAGACTGA

Upstream 100 bases:

>100_bases
AAATTTTTGCAATAATTCTTATCATAAAACTTTATAAAATTGTATTAAGACTTTGTCCCATACACTATTTTGCTAAAGAG
GCTAAATTCTATTAATTGCG

Downstream 100 bases:

>100_bases
TGGATTTGTTAGATGCTTGGGATTACTTCATAAACGAGGTTAGAAAATTTTTCAAAGAGAAGGGCTACACCGAGGTCTCC
ACCCCCCTCCTTCTCGACTT

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 617; Mature: 616

Protein sequence:

>617_residues
MAWVVDEFDVVVIGGGHAGIEAALAAARMGAKTAMFVLNADTIGQMSCNPAIGGIAKGIVVREIDALGGEMGKAIDQTGI
QFKMLNTRKGKAVQSPRAQADKKRYREYMKKVCENQENLYIKQEEVVDIIVKNNQVVGVRTNLGVEYKTKAVVVTTGTFL
NGVIYIGDKMIPGGRLGEPRSEGLSDFYRRFDFPLIRFKTGTPARLDKRTIDFSALEVAPGDDPPPKFSFWTEPVGSYWF
PKGKEQVNCWITYTTPKTHEIIRKNLHRTALYGGLIKGIGPRYCPSIEDKIVKFPDKERHQIFLEPEGLDTIEIYPNGLS
TSLPEEVQWEMYRSIPGLENVVLIRPAYAIEYDVVPPTELYPTLETKKIRGLFHAGNFNGTTGYEEAAGQGIVAGINAAL
RAFGKEPIYLRRDESYIGVMIDDLTTKGVTEPYRLFTSRSEYRLYIRQDNAILRLAKLGRELGLLSEEQYKLVKELEREI
EKWKEFYKSERVSVAVGGDTRSYSVATLMTMNYTLDDVKEKFGYEVPQHPYVKEEVEIQLKYEPYIERERKLNEKLKKLE
DTKIPPDIDYDKIPGLTKEAREKLKKFKPITVGQASRIDGITPAAITALLVYLGKLD

Sequences:

>Translated_617_residues
MAWVVDEFDVVVIGGGHAGIEAALAAARMGAKTAMFVLNADTIGQMSCNPAIGGIAKGIVVREIDALGGEMGKAIDQTGI
QFKMLNTRKGKAVQSPRAQADKKRYREYMKKVCENQENLYIKQEEVVDIIVKNNQVVGVRTNLGVEYKTKAVVVTTGTFL
NGVIYIGDKMIPGGRLGEPRSEGLSDFYRRFDFPLIRFKTGTPARLDKRTIDFSALEVAPGDDPPPKFSFWTEPVGSYWF
PKGKEQVNCWITYTTPKTHEIIRKNLHRTALYGGLIKGIGPRYCPSIEDKIVKFPDKERHQIFLEPEGLDTIEIYPNGLS
TSLPEEVQWEMYRSIPGLENVVLIRPAYAIEYDVVPPTELYPTLETKKIRGLFHAGNFNGTTGYEEAAGQGIVAGINAAL
RAFGKEPIYLRRDESYIGVMIDDLTTKGVTEPYRLFTSRSEYRLYIRQDNAILRLAKLGRELGLLSEEQYKLVKELEREI
EKWKEFYKSERVSVAVGGDTRSYSVATLMTMNYTLDDVKEKFGYEVPQHPYVKEEVEIQLKYEPYIERERKLNEKLKKLE
DTKIPPDIDYDKIPGLTKEAREKLKKFKPITVGQASRIDGITPAAITALLVYLGKLD
>Mature_616_residues
AWVVDEFDVVVIGGGHAGIEAALAAARMGAKTAMFVLNADTIGQMSCNPAIGGIAKGIVVREIDALGGEMGKAIDQTGIQ
FKMLNTRKGKAVQSPRAQADKKRYREYMKKVCENQENLYIKQEEVVDIIVKNNQVVGVRTNLGVEYKTKAVVVTTGTFLN
GVIYIGDKMIPGGRLGEPRSEGLSDFYRRFDFPLIRFKTGTPARLDKRTIDFSALEVAPGDDPPPKFSFWTEPVGSYWFP
KGKEQVNCWITYTTPKTHEIIRKNLHRTALYGGLIKGIGPRYCPSIEDKIVKFPDKERHQIFLEPEGLDTIEIYPNGLST
SLPEEVQWEMYRSIPGLENVVLIRPAYAIEYDVVPPTELYPTLETKKIRGLFHAGNFNGTTGYEEAAGQGIVAGINAALR
AFGKEPIYLRRDESYIGVMIDDLTTKGVTEPYRLFTSRSEYRLYIRQDNAILRLAKLGRELGLLSEEQYKLVKELEREIE
KWKEFYKSERVSVAVGGDTRSYSVATLMTMNYTLDDVKEKFGYEVPQHPYVKEEVEIQLKYEPYIERERKLNEKLKKLED
TKIPPDIDYDKIPGLTKEAREKLKKFKPITVGQASRIDGITPAAITALLVYLGKLD

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=639, Percent_Identity=42.566510172144, Blast_Score=457, Evalue=1e-128,
Organism=Homo sapiens, GI19882217, Length=664, Percent_Identity=40.9638554216867, Blast_Score=443, Evalue=1e-124,
Organism=Homo sapiens, GI183227703, Length=679, Percent_Identity=40.0589101620029, Blast_Score=438, Evalue=1e-123,
Organism=Escherichia coli, GI2367273, Length=623, Percent_Identity=51.2038523274478, Blast_Score=609, Evalue=1e-175,
Organism=Caenorhabditis elegans, GI17534255, Length=622, Percent_Identity=40.5144694533762, Blast_Score=452, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6321202, Length=631, Percent_Identity=44.5324881141046, Blast_Score=516, Evalue=1e-147,
Organism=Drosophila melanogaster, GI24658174, Length=637, Percent_Identity=41.4442700156986, Blast_Score=466, Evalue=1e-131,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_AQUAE (O66962)

Other databases:

- EMBL:   AE000657
- PIR:   G70366
- RefSeq:   NP_213523.1
- PDB:   2ZXH
- PDB:   2ZXI
- PDBsum:   2ZXH
- PDBsum:   2ZXI
- ProteinModelPortal:   O66962
- SMR:   O66962
- DIP:   DIP-48311N
- GeneID:   1193412
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_761
- NMPDR:   fig|224324.1.peg.538
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- PhylomeDB:   O66962
- ProtClustDB:   PRK05192
- BioCyc:   AAEO224324:AQ_761-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR013027
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- PRINTS:   PR00368
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 69645; Mature: 69514

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: BINDING 126-126 BINDING 181-181 BINDING 377-377

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWVVDEFDVVVIGGGHAGIEAALAAARMGAKTAMFVLNADTIGQMSCNPAIGGIAKGIV
CCEEEECCCEEEEECCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHH
VREIDALGGEMGKAIDQTGIQFKMLNTRKGKAVQSPRAQADKKRYREYMKKVCENQENLY
HHHHHHHCHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEE
IKQEEVVDIIVKNNQVVGVRTNLGVEYKTKAVVVTTGTFLNGVIYIGDKMIPGGRLGEPR
EEHHHEEEEEEECCEEEEEEECCCCEEEEEEEEEEECHHHCCEEEECCEECCCCCCCCCH
SEGLSDFYRRFDFPLIRFKTGTPARLDKRTIDFSALEVAPGDDPPPKFSFWTEPVGSYWF
HHHHHHHHHHCCCCEEEEECCCCCCCCCCEECEEEEEECCCCCCCCCCCCCCCCCCCCCC
PKGKEQVNCWITYTTPKTHEIIRKNLHRTALYGGLIKGIGPRYCPSIEDKIVKFPDKERH
CCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCC
QIFLEPEGLDTIEIYPNGLSTSLPEEVQWEMYRSIPGLENVVLIRPAYAIEYDVVPPTEL
EEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCEEEEECCCCCHHH
YPTLETKKIRGLFHAGNFNGTTGYEEAAGQGIVAGINAALRAFGKEPIYLRRDESYIGVM
CCCCCHHHHHEEEECCCCCCCCCCHHHCCCCEEEHHHHHHHHHCCCCEEEEECCCEEEEE
IDDLTTKGVTEPYRLFTSRSEYRLYIRQDNAILRLAKLGRELGLLSEEQYKLVKELEREI
EECCCCCCCCCHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
EKWKEFYKSERVSVAVGGDTRSYSVATLMTMNYTLDDVKEKFGYEVPQHPYVKEEVEIQL
HHHHHHHHCCCEEEEECCCCCCEEEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCEEEEE
KYEPYIERERKLNEKLKKLEDTKIPPDIDYDKIPGLTKEAREKLKKFKPITVGQASRIDG
EECCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCC
ITPAAITALLVYLGKLD
CCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AWVVDEFDVVVIGGGHAGIEAALAAARMGAKTAMFVLNADTIGQMSCNPAIGGIAKGIV
CEEEECCCEEEEECCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHH
VREIDALGGEMGKAIDQTGIQFKMLNTRKGKAVQSPRAQADKKRYREYMKKVCENQENLY
HHHHHHHCHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEE
IKQEEVVDIIVKNNQVVGVRTNLGVEYKTKAVVVTTGTFLNGVIYIGDKMIPGGRLGEPR
EEHHHEEEEEEECCEEEEEEECCCCEEEEEEEEEEECHHHCCEEEECCEECCCCCCCCCH
SEGLSDFYRRFDFPLIRFKTGTPARLDKRTIDFSALEVAPGDDPPPKFSFWTEPVGSYWF
HHHHHHHHHHCCCCEEEEECCCCCCCCCCEECEEEEEECCCCCCCCCCCCCCCCCCCCCC
PKGKEQVNCWITYTTPKTHEIIRKNLHRTALYGGLIKGIGPRYCPSIEDKIVKFPDKERH
CCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCC
QIFLEPEGLDTIEIYPNGLSTSLPEEVQWEMYRSIPGLENVVLIRPAYAIEYDVVPPTEL
EEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCEEEEECCCCCHHH
YPTLETKKIRGLFHAGNFNGTTGYEEAAGQGIVAGINAALRAFGKEPIYLRRDESYIGVM
CCCCCHHHHHEEEECCCCCCCCCCHHHCCCCEEEHHHHHHHHHCCCCEEEEECCCEEEEE
IDDLTTKGVTEPYRLFTSRSEYRLYIRQDNAILRLAKLGRELGLLSEEQYKLVKELEREI
EECCCCCCCCCHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
EKWKEFYKSERVSVAVGGDTRSYSVATLMTMNYTLDDVKEKFGYEVPQHPYVKEEVEIQL
HHHHHHHHCCCEEEEECCCCCCEEEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCEEEEE
KYEPYIERERKLNEKLKKLEDTKIPPDIDYDKIPGLTKEAREKLKKFKPITVGQASRIDG
EECCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCC
ITPAAITALLVYLGKLD
CCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320