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The map label for this gene is lpdA [H]

Identifier: 15606129

GI number: 15606129

Start: 510161

End: 511558

Strand: Direct

Name: lpdA [H]

Synonym: aq_736

Alternate gene names: 15606129

Gene position: 510161-511558 (Clockwise)

Preceding gene: 15606126

Following gene: 15606130

Centisome position: 32.89

GC content: 48.28

Gene sequence:

>1398_bases
ATGGAGTTTGACTTAATAATCGTGGGTGCGGGTAGTGGAGGTTACGAGGCGGGGCTTTACGCCTTCAGAAGGGGAATGAA
GGTAGCCTTCGTGGAACTCTCTCCGGAGACCGTGGGAGGAAATTGCCTCAACAGAGGTTGCATCCCTTCAAAGTACATGA
GGCACGGGGCTTACCTCCTTGACAAGTTTCAGAAAATGGAGCAATACGGAATAATTTCAAAGGGTTACGACATAGAGTAC
AAAAAACTCAAAGAGGGAAGGGATAACGTCGTAGTGACCATAAGGGAAAACTTTAAGAAGTTCGCCCAGCAACTCAGAAT
TCCCATATACTACGGCAAAGGAGTTTTAAAGGATCCAAACACTGTGTTCGTGGAAGGACCAGAGGAAACTCTGAAGGCTA
AGTACATCCTCGTGGCTACTGGATCCTCTCCTACCTCTGTCGGGAACCTCGTTCCGGACGGAAAGTACGTTATAGACACA
GATCAGATATGGGAAATAGATTACGTACCAAAGAAGGTTCTAATAGTCGGGGGCGGAGCAGTAGGGGTTGAGTTCGCATA
CATATTCAGGAAGTACGGAAGTGAGGTTGTTCTGGTAGAGATAAAAGACAGACTCCTTCCCACTCCCGATATCCCCGAGG
ACAGCGGGAGGTACCTCGCCAGAAAGTTAAGGGAACTGGGAGTTGATATAAGGACGAGAACTTCTGTGGAGAGCTGGGAA
AAGACTCAGAACGGAGTAAAGGCAAAGCTCACGGACGGGAGCGAGGTTGTAGCGGACTTCATCCTCCTCGGTGTAGGAAG
AAAACCAAACACCAAAGGAATAGGCCTTGAGGAACTCGGTATAGAGATGGACGAGAGGGGATTCGTAAAGACGAACGAGT
ACGCCCAAACGAACATTCCAAACATATACGCCTGTGGGGATATAACCTCTCCCTTAATGCTGGCTCACAAGTCTATGTAC
GAGGGTAAAATCGCCGTTTCCCACATACTTGGCGAGAGGGACTGGAAGAAGAACGAGAGGATAATTCCCAAGATTATTTA
CTCCGCACTGGAAGTGGCATCGGTCGGCCTCACTGAAGAGCAGGCGGAAGACGAAGACATAGAAGTAAGGGTGGGAGTAG
CTTCTTTCGTTTCAAACCCCAAAGCCATGGACGACGGAGAGAATGAGGGATTTGTAAGGATAGTGGCGGACGACGAGACT
GGTGAAATACTCGGATGTCACATAGTCGGTCCACATGCGGGAGAACTCATACATCAGGTAGTTCACATGATAAAGGACGG
AAAAACCGTGGAGTTTGCTTCAAAGACTATGTACTCCCACCCATCACTTTCGGAAAACATAGGAATAGCTTCCTCGGAGG
TTTATTACGGACCTATATCGTGGGTAAAAAGGCGGTAA

Upstream 100 bases:

>100_bases
GTTGAACGTCGGCATTCGCTTCCTCCTTTTAAGGCAAATTATTAGTATATCAAAATGCTAAAATTAAAGGCAATTAAAAC
CCCTTTTCAGGAGGAGCTTC

Downstream 100 bases:

>100_bases
AACTTGACACCACTCATTGTTTATTCAAAGGGAATACTCATATTTCCTAAAGGAGGTAAGTAAATGGCCGTTCAGGACGT
AATAGAGGCTCTCAAGAAGG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MEFDLIIVGAGSGGYEAGLYAFRRGMKVAFVELSPETVGGNCLNRGCIPSKYMRHGAYLLDKFQKMEQYGIISKGYDIEY
KKLKEGRDNVVVTIRENFKKFAQQLRIPIYYGKGVLKDPNTVFVEGPEETLKAKYILVATGSSPTSVGNLVPDGKYVIDT
DQIWEIDYVPKKVLIVGGGAVGVEFAYIFRKYGSEVVLVEIKDRLLPTPDIPEDSGRYLARKLRELGVDIRTRTSVESWE
KTQNGVKAKLTDGSEVVADFILLGVGRKPNTKGIGLEELGIEMDERGFVKTNEYAQTNIPNIYACGDITSPLMLAHKSMY
EGKIAVSHILGERDWKKNERIIPKIIYSALEVASVGLTEEQAEDEDIEVRVGVASFVSNPKAMDDGENEGFVRIVADDET
GEILGCHIVGPHAGELIHQVVHMIKDGKTVEFASKTMYSHPSLSENIGIASSEVYYGPISWVKRR

Sequences:

>Translated_465_residues
MEFDLIIVGAGSGGYEAGLYAFRRGMKVAFVELSPETVGGNCLNRGCIPSKYMRHGAYLLDKFQKMEQYGIISKGYDIEY
KKLKEGRDNVVVTIRENFKKFAQQLRIPIYYGKGVLKDPNTVFVEGPEETLKAKYILVATGSSPTSVGNLVPDGKYVIDT
DQIWEIDYVPKKVLIVGGGAVGVEFAYIFRKYGSEVVLVEIKDRLLPTPDIPEDSGRYLARKLRELGVDIRTRTSVESWE
KTQNGVKAKLTDGSEVVADFILLGVGRKPNTKGIGLEELGIEMDERGFVKTNEYAQTNIPNIYACGDITSPLMLAHKSMY
EGKIAVSHILGERDWKKNERIIPKIIYSALEVASVGLTEEQAEDEDIEVRVGVASFVSNPKAMDDGENEGFVRIVADDET
GEILGCHIVGPHAGELIHQVVHMIKDGKTVEFASKTMYSHPSLSENIGIASSEVYYGPISWVKRR
>Mature_465_residues
MEFDLIIVGAGSGGYEAGLYAFRRGMKVAFVELSPETVGGNCLNRGCIPSKYMRHGAYLLDKFQKMEQYGIISKGYDIEY
KKLKEGRDNVVVTIRENFKKFAQQLRIPIYYGKGVLKDPNTVFVEGPEETLKAKYILVATGSSPTSVGNLVPDGKYVIDT
DQIWEIDYVPKKVLIVGGGAVGVEFAYIFRKYGSEVVLVEIKDRLLPTPDIPEDSGRYLARKLRELGVDIRTRTSVESWE
KTQNGVKAKLTDGSEVVADFILLGVGRKPNTKGIGLEELGIEMDERGFVKTNEYAQTNIPNIYACGDITSPLMLAHKSMY
EGKIAVSHILGERDWKKNERIIPKIIYSALEVASVGLTEEQAEDEDIEVRVGVASFVSNPKAMDDGENEGFVRIVADDET
GEILGCHIVGPHAGELIHQVVHMIKDGKTVEFASKTMYSHPSLSENIGIASSEVYYGPISWVKRR

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=468, Percent_Identity=31.6239316239316, Blast_Score=235, Evalue=7e-62,
Organism=Homo sapiens, GI50301238, Length=462, Percent_Identity=27.9220779220779, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI33519430, Length=455, Percent_Identity=25.4945054945055, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI33519428, Length=455, Percent_Identity=25.4945054945055, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI33519426, Length=455, Percent_Identity=25.4945054945055, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI148277065, Length=455, Percent_Identity=26.1538461538462, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI148277071, Length=455, Percent_Identity=25.4945054945055, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI291045266, Length=456, Percent_Identity=26.9736842105263, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI22035672, Length=459, Percent_Identity=25.9259259259259, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI291045268, Length=451, Percent_Identity=25.2771618625277, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI65787454, Length=223, Percent_Identity=27.8026905829596, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI21389617, Length=223, Percent_Identity=27.8026905829596, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI226437568, Length=223, Percent_Identity=27.8026905829596, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1786307, Length=467, Percent_Identity=28.4796573875803, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI87082354, Length=456, Percent_Identity=30.9210526315789, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI87081717, Length=455, Percent_Identity=27.2527472527473, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1789915, Length=428, Percent_Identity=26.6355140186916, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1789065, Length=240, Percent_Identity=27.5, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789765, Length=247, Percent_Identity=27.1255060728745, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI32565766, Length=471, Percent_Identity=32.9087048832272, Blast_Score=246, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI17557007, Length=476, Percent_Identity=26.890756302521, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71983429, Length=369, Percent_Identity=26.8292682926829, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71983419, Length=369, Percent_Identity=26.8292682926829, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71982272, Length=491, Percent_Identity=25.050916496945, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI32564386, Length=143, Percent_Identity=33.5664335664336, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17559934, Length=194, Percent_Identity=29.3814432989691, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=470, Percent_Identity=31.063829787234, Blast_Score=208, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6325240, Length=466, Percent_Identity=26.6094420600858, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6325166, Length=464, Percent_Identity=25.2155172413793, Blast_Score=137, Evalue=3e-33,
Organism=Drosophila melanogaster, GI21358499, Length=471, Percent_Identity=32.9087048832272, Blast_Score=255, Evalue=5e-68,
Organism=Drosophila melanogaster, GI24640553, Length=474, Percent_Identity=26.3713080168776, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24640549, Length=474, Percent_Identity=26.3713080168776, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24640551, Length=474, Percent_Identity=26.3713080168776, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI17737741, Length=475, Percent_Identity=25.4736842105263, Blast_Score=111, Evalue=8e-25,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 51565; Mature: 51565

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFDLIIVGAGSGGYEAGLYAFRRGMKVAFVELSPETVGGNCLNRGCIPSKYMRHGAYLL
CCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCHHCCCCCCHHHHHHHHHHH
DKFQKMEQYGIISKGYDIEYKKLKEGRDNVVVTIRENFKKFAQQLRIPIYYGKGVLKDPN
HHHHHHHHHCCCCCCCCCCHHHHHCCCCCEEEEEHHHHHHHHHHCCCEEEECCCEECCCC
TVFVEGPEETLKAKYILVATGSSPTSVGNLVPDGKYVIDTDQIWEIDYVPKKVLIVGGGA
EEEEECCHHHCCEEEEEEEECCCCCCCCCCCCCCCEEECCCCEEEEECCCCEEEEEECCH
VGVEFAYIFRKYGSEVVLVEIKDRLLPTPDIPEDSGRYLARKLRELGVDIRTRTSVESWE
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCHHHHH
KTQNGVKAKLTDGSEVVADFILLGVGRKPNTKGIGLEELGIEMDERGFVKTNEYAQTNIP
HHCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHCCEECCCCCEECCCCCCCCCC
NIYACGDITSPLMLAHKSMYEGKIAVSHILGERDWKKNERIIPKIIYSALEVASVGLTEE
CEEECCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHH
QAEDEDIEVRVGVASFVSNPKAMDDGENEGFVRIVADDETGEILGCHIVGPHAGELIHQV
CCCCCCEEEEEEHHHHHCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCHHHHHHHH
VHMIKDGKTVEFASKTMYSHPSLSENIGIASSEVYYGPISWVKRR
HHHHCCCCEEEEHHHHHCCCCCCCCCCCCCCCCEEECCHHHHCCC
>Mature Secondary Structure
MEFDLIIVGAGSGGYEAGLYAFRRGMKVAFVELSPETVGGNCLNRGCIPSKYMRHGAYLL
CCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCHHCCCCCCHHHHHHHHHHH
DKFQKMEQYGIISKGYDIEYKKLKEGRDNVVVTIRENFKKFAQQLRIPIYYGKGVLKDPN
HHHHHHHHHCCCCCCCCCCHHHHHCCCCCEEEEEHHHHHHHHHHCCCEEEECCCEECCCC
TVFVEGPEETLKAKYILVATGSSPTSVGNLVPDGKYVIDTDQIWEIDYVPKKVLIVGGGA
EEEEECCHHHCCEEEEEEEECCCCCCCCCCCCCCCEEECCCCEEEEECCCCEEEEEECCH
VGVEFAYIFRKYGSEVVLVEIKDRLLPTPDIPEDSGRYLARKLRELGVDIRTRTSVESWE
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCHHHHH
KTQNGVKAKLTDGSEVVADFILLGVGRKPNTKGIGLEELGIEMDERGFVKTNEYAQTNIP
HHCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHCCEECCCCCEECCCCCCCCCC
NIYACGDITSPLMLAHKSMYEGKIAVSHILGERDWKKNERIIPKIIYSALEVASVGLTEE
CEEECCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHH
QAEDEDIEVRVGVASFVSNPKAMDDGENEGFVRIVADDETGEILGCHIVGPHAGELIHQV
CCCCCCEEEEEEHHHHHCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCHHHHHHHH
VHMIKDGKTVEFASKTMYSHPSLSENIGIASSEVYYGPISWVKRR
HHHHCCCCEEEEHHHHHCCCCCCCCCCCCCCCCEEECCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]