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The map label for this gene is hisH

Identifier: 15606126

GI number: 15606126

Start: 508590

End: 509213

Strand: Direct

Name: hisH

Synonym: aq_732

Alternate gene names: 15606126

Gene position: 508590-509213 (Clockwise)

Preceding gene: 15606125

Following gene: 15606129

Centisome position: 32.78

GC content: 44.71

Gene sequence:

>624_bases
ATGAGGACTGTTGTAATAGACTATGGAATGGGCAACTTAAGGAGTGTCAGTAAAGCTCTTGAGGCGGTAGGATTTCCCGA
AGTAGTAGTCTCAAACGATTACAGAGTGGCTTCCGAGGCGGATGTCCTTGTTCTTCCGGGTGTCGGTGCCTTCGGAGACG
CTATGAAAAATCTGGAGGAACTAAACTTAGTCAGCGTAATAAGGAGACATATAGAAAAAGGAAAGCCCTTTCTTGGAATA
TGCCTCGGTCTTCAACTCCTCTTTGAAAAAAGCTATGAACACGGTGAGCATAGGGGTCTCGGGATTTTAAAGGGTGAGGT
TATACTGCTCCCTTTGGGGGTGAAAATACCCCACATAGGATGGAACCAGCTGTGGTTTAAGAAGGAAAGTGAGATACTGG
AAGGTCTGAAAGAGGGAGATTTCGTTTACTTCGTCCACTCTTACAGAGTAGTTCCGGAAGACGAGAGCGTAGTTCTCACA
AAAACCGATTACGGAGAATACTTCGTCTCTTCAATAGAACTGGATAACGTAGTGGCTTTCCAGTTCCACCCCGAAAAGAG
TCAAAAGAAGGGCTTAAAACTCCTTGAAAATTTTAAAAGGAAGGCGGAAAAGCTTACCACCTGA

Upstream 100 bases:

>100_bases
ACTTCAAGTACGTGGAAATTGCGGGCGGTGTTCTGCTTGTTTCCTTGGGACTTCTTCTCGCACTGGATAAGCTCTCACTT
ATTGCTAACATTACTTTCTG

Downstream 100 bases:

>100_bases
AGTGGGAGAAAACCATGTTCGCATGTGCCATCCTGTGTGTCTCTTCCTTCTTCTTGTACGCTCCTCCCCTCTCGTTCAGC
GCATCCAATAGCTCCGCCTT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MRTVVIDYGMGNLRSVSKALEAVGFPEVVVSNDYRVASEADVLVLPGVGAFGDAMKNLEELNLVSVIRRHIEKGKPFLGI
CLGLQLLFEKSYEHGEHRGLGILKGEVILLPLGVKIPHIGWNQLWFKKESEILEGLKEGDFVYFVHSYRVVPEDESVVLT
KTDYGEYFVSSIELDNVVAFQFHPEKSQKKGLKLLENFKRKAEKLTT

Sequences:

>Translated_207_residues
MRTVVIDYGMGNLRSVSKALEAVGFPEVVVSNDYRVASEADVLVLPGVGAFGDAMKNLEELNLVSVIRRHIEKGKPFLGI
CLGLQLLFEKSYEHGEHRGLGILKGEVILLPLGVKIPHIGWNQLWFKKESEILEGLKEGDFVYFVHSYRVVPEDESVVLT
KTDYGEYFVSSIELDNVVAFQFHPEKSQKKGLKLLENFKRKAEKLTT
>Mature_207_residues
MRTVVIDYGMGNLRSVSKALEAVGFPEVVVSNDYRVASEADVLVLPGVGAFGDAMKNLEELNLVSVIRRHIEKGKPFLGI
CLGLQLLFEKSYEHGEHRGLGILKGEVILLPLGVKIPHIGWNQLWFKKESEILEGLKEGDFVYFVHSYRVVPEDESVVLT
KTDYGEYFVSSIELDNVVAFQFHPEKSQKKGLKLLENFKRKAEKLTT

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=199, Percent_Identity=38.6934673366834, Blast_Score=134, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6319725, Length=209, Percent_Identity=35.4066985645933, Blast_Score=115, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_AQUAE (O66943)

Other databases:

- EMBL:   AE000657
- PIR:   C70364
- RefSeq:   NP_213503.1
- ProteinModelPortal:   O66943
- SMR:   O66943
- GeneID:   1193392
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_732
- NMPDR:   fig|224324.1.peg.518
- HOGENOM:   HBG292341
- OMA:   ASENFED
- PhylomeDB:   O66943
- ProtClustDB:   PRK13141
- BioCyc:   AAEO224324:AQ_732-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 23307; Mature: 23307

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 81-81 ACT_SITE 183-183 ACT_SITE 185-185

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTVVIDYGMGNLRSVSKALEAVGFPEVVVSNDYRVASEADVLVLPGVGAFGDAMKNLEE
CEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCEEECCCCCEEEECCCCHHHHHHHHHHH
LNLVSVIRRHIEKGKPFLGICLGLQLLFEKSYEHGEHRGLGILKGEVILLPLGVKIPHIG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCCCCC
WNQLWFKKESEILEGLKEGDFVYFVHSYRVVPEDESVVLTKTDYGEYFVSSIELDNVVAF
CCHHHCCCHHHHHHHHCCCCEEEEEEEEEECCCCCCEEEEECCCCHHHHHHCCCCCEEEE
QFHPEKSQKKGLKLLENFKRKAEKLTT
EECCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRTVVIDYGMGNLRSVSKALEAVGFPEVVVSNDYRVASEADVLVLPGVGAFGDAMKNLEE
CEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCEEECCCCCEEEECCCCHHHHHHHHHHH
LNLVSVIRRHIEKGKPFLGICLGLQLLFEKSYEHGEHRGLGILKGEVILLPLGVKIPHIG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCCCCC
WNQLWFKKESEILEGLKEGDFVYFVHSYRVVPEDESVVLTKTDYGEYFVSSIELDNVVAF
CCHHHCCCHHHHHHHHCCCCEEEEEEEEEECCCCCCEEEEECCCCHHHHHHCCCCCEEEE
QFHPEKSQKKGLKLLENFKRKAEKLTT
EECCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320