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The map label for this gene is hypF [H]

Identifier: 15606085

GI number: 15606085

Start: 457083

End: 459323

Strand: Direct

Name: hypF [H]

Synonym: aq_672

Alternate gene names: 15606085

Gene position: 457083-459323 (Clockwise)

Preceding gene: 15606084

Following gene: 15606086

Centisome position: 29.46

GC content: 44.67

Gene sequence:

>2241_bases
ATGCCCTTCAAAAGGCTAAAGCTTCACCTTAAAGGGGCCGTTCAGGGTGTCGGGTTTCGCCCCTTCGTTTACAGAATAGC
CAAAGAGCTGGGACTAAAAGGTTTCGTTATAAACGACTCAAAAGGTGTTTACATAGAAGTAGAAGGAGAAGAGGAAAGGT
TAAAGAAATTTCTTTTTAAACTAAACAGGGAAAAACCGCCCCTTGCGAGAATTTACTCTCAGGAAATCCAGTTTTTAGAG
CCTGTAAATTACGAGGACTTCGTAATAAGAAAAAGCGAGGAGAAAGGTGAGAAGGAAGTCTTAGTTCTCCCGGACATAGC
AACGTGTGAGGACTGCCTAAGAGAACTCTTTACCCCTGAAGACAGGAGGTATATGTACCCCTTCATAAACTGCACCAACT
GCGGTCCGAGGTTCACTATAATCGAAAGACTGCCCTATGACAGAAAAAACACCACCATGAAAGTCTTTGAAATGTGTCCC
GAGTGCAAAAGGGAGTATGAGAACCCCCTCGACAGACGCTTTCATGCTCAGCCAAACGCCTGCCCGAAGTGCGGTCCATG
GGTCAGCCTCTACAAAGACGGTAAACTAATTGCCGAAAAAAATGAGGCTTTAGAACTCTTAATTGAAGAAATAAAAATAG
GAAAAATAGTAGCAGTTAAGGGAGTAGGAGGTTTTCACCTTATATGCAACGCAACGAACGAAGAGAGCGTAAGAACCCTC
AGGAAGAGAAAGAGGAGAAGTGAAAAGCCCTTCGCGGTAATGTTTAAAAGTCTTGAACAAGTTGAAGCATATGCAAACCC
TACCGAACTTGAAAAGGCACTCTTGATTTCCCCGGAAAGACCTATAGTATTAATACAAAAGAAGAAGGAACTCGCTCCAT
CCGTATCGCCCGGATTAAAAAGAGTTGGTGCGTTTCTCCCCTATTCTCCCCTTCACCACCTTATACTGAACTCCTTAGAT
TTTCCCGTTGTGGCGACTTCTGCAAATATCTCAGAAGAGCCCATAATCAAAGACAACAAGGAAGCACTGGAAAAACTGGG
AGAGCTCGCCGATCTTATCCTCGTTCACAACAGGGATATAAAAAGGAGGTGTGACGACTCGGTAGTTAAAGTCGTGAGTG
GTGTTCCTACACCGATAAGACGATCAAGGGGCTACGCACCACTTCCCGTAGAAGTTCCTTTTGAACTTCCCAAAAGAGTT
CTTGCGGTTGGAGGAATGCTAAAAAACACCTTTGCCCTCGGCTTTAAAAACCAAGTAATCCTGAGTCAGCACATCGGGGA
TATTGAGAACTTAAATACTTTAAAGGTTTTTGAAGAAAGTGTTTTTGACCTTATGGAACTCTACGAGTTTGAGCCCGATG
TAGTAGTTTGCGATATGCACCCGAGGTACGAAACTACGAGGTGGGCTGAGGAGTTTTCCAGAAAAAGGGGAATACCCTTA
ATTAAGGTTCAGCACCACTACGCCCACATGCTCTCCTGCATGGCGGAAAACGGGATAAAAGAAAAGGTTCTCGGAATAGC
TTGGGACGGAACGGGATACGGAGAGGACGGAACCCTCTGGGGAGGTGAGTTTTTAGTGGCAGATTACACTTCTTATGAAA
GAGCATTTAACTTCAAACCCGTAAAACTCATAGGCGGAGAGAAGGCGGTAAAGGAACCGAGGAGGGTAGCCCTCTCTTTA
CTCTTTGATATATTCGGGGAGGAAGCCCTCAACTTGGACCTTCTCCCGGTAAAAAGCTTTAGCGAAAGGGAGCTAAAAAA
CCTGTACCTTGCCTGGAAAAAAGGCATAAACTCACCGCTTAGTTCTTCTGTCGGAAGACTTTTTGATGCTTTGGCTTCCC
TTCTTAATCTCAAACAAATCCTCTCTTACGAAGGGCAGGGAGCCATGATGGTGGAAGATTTATACGACCCTCTTGTAAAG
GATAACTATCCCTATGAGATACGCGGAAAGGAAGTAGATTTAAGGAAGGCCTTCCTAGAAGTTTTAAAGGAAAAAGACAA
GAGTCTCGCGGCAAGCAGGTTTATAAACACGCTCGCGAAGGTTTGCGAAGACATAGCCCTCATGGTTGGTATTGAACGTG
TGTGCCTTTCTGGGGGTGTTATGCAAAACGATCCACTGGTTACAAAGATAAAGGAGCTTCTGGAAAAGGAAAAATTTAAG
GTTTACACGCACCAAAAGGTTCCTCCGAACGACGGAGGTATAGCTCTGGGACAGGCTGTTTTTGGACTTTCTCTGGTATA
A

Upstream 100 bases:

>100_bases
GTAAATCCTTCCCTTGACGTCATTACGCTTTCCGCAAAAACTGGAGAGGGAATGGAATTGTGGCTTGATTATCTGAAGTT
TAAGTTGAAGGTCCTCAGGA

Downstream 100 bases:

>100_bases
TTTAGGGATTATGATACAGGTTGAAATAGTTTCTCCGCAGGGAATGGTTTACTCGGGAGAAGTAGAGAGCGTAAACGTCC
CCACGGTTGAAGGAGAGGTG

Product: transcriptional regulatory protein HypF

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 746; Mature: 745

Protein sequence:

>746_residues
MPFKRLKLHLKGAVQGVGFRPFVYRIAKELGLKGFVINDSKGVYIEVEGEEERLKKFLFKLNREKPPLARIYSQEIQFLE
PVNYEDFVIRKSEEKGEKEVLVLPDIATCEDCLRELFTPEDRRYMYPFINCTNCGPRFTIIERLPYDRKNTTMKVFEMCP
ECKREYENPLDRRFHAQPNACPKCGPWVSLYKDGKLIAEKNEALELLIEEIKIGKIVAVKGVGGFHLICNATNEESVRTL
RKRKRRSEKPFAVMFKSLEQVEAYANPTELEKALLISPERPIVLIQKKKELAPSVSPGLKRVGAFLPYSPLHHLILNSLD
FPVVATSANISEEPIIKDNKEALEKLGELADLILVHNRDIKRRCDDSVVKVVSGVPTPIRRSRGYAPLPVEVPFELPKRV
LAVGGMLKNTFALGFKNQVILSQHIGDIENLNTLKVFEESVFDLMELYEFEPDVVVCDMHPRYETTRWAEEFSRKRGIPL
IKVQHHYAHMLSCMAENGIKEKVLGIAWDGTGYGEDGTLWGGEFLVADYTSYERAFNFKPVKLIGGEKAVKEPRRVALSL
LFDIFGEEALNLDLLPVKSFSERELKNLYLAWKKGINSPLSSSVGRLFDALASLLNLKQILSYEGQGAMMVEDLYDPLVK
DNYPYEIRGKEVDLRKAFLEVLKEKDKSLAASRFINTLAKVCEDIALMVGIERVCLSGGVMQNDPLVTKIKELLEKEKFK
VYTHQKVPPNDGGIALGQAVFGLSLV

Sequences:

>Translated_746_residues
MPFKRLKLHLKGAVQGVGFRPFVYRIAKELGLKGFVINDSKGVYIEVEGEEERLKKFLFKLNREKPPLARIYSQEIQFLE
PVNYEDFVIRKSEEKGEKEVLVLPDIATCEDCLRELFTPEDRRYMYPFINCTNCGPRFTIIERLPYDRKNTTMKVFEMCP
ECKREYENPLDRRFHAQPNACPKCGPWVSLYKDGKLIAEKNEALELLIEEIKIGKIVAVKGVGGFHLICNATNEESVRTL
RKRKRRSEKPFAVMFKSLEQVEAYANPTELEKALLISPERPIVLIQKKKELAPSVSPGLKRVGAFLPYSPLHHLILNSLD
FPVVATSANISEEPIIKDNKEALEKLGELADLILVHNRDIKRRCDDSVVKVVSGVPTPIRRSRGYAPLPVEVPFELPKRV
LAVGGMLKNTFALGFKNQVILSQHIGDIENLNTLKVFEESVFDLMELYEFEPDVVVCDMHPRYETTRWAEEFSRKRGIPL
IKVQHHYAHMLSCMAENGIKEKVLGIAWDGTGYGEDGTLWGGEFLVADYTSYERAFNFKPVKLIGGEKAVKEPRRVALSL
LFDIFGEEALNLDLLPVKSFSERELKNLYLAWKKGINSPLSSSVGRLFDALASLLNLKQILSYEGQGAMMVEDLYDPLVK
DNYPYEIRGKEVDLRKAFLEVLKEKDKSLAASRFINTLAKVCEDIALMVGIERVCLSGGVMQNDPLVTKIKELLEKEKFK
VYTHQKVPPNDGGIALGQAVFGLSLV
>Mature_745_residues
PFKRLKLHLKGAVQGVGFRPFVYRIAKELGLKGFVINDSKGVYIEVEGEEERLKKFLFKLNREKPPLARIYSQEIQFLEP
VNYEDFVIRKSEEKGEKEVLVLPDIATCEDCLRELFTPEDRRYMYPFINCTNCGPRFTIIERLPYDRKNTTMKVFEMCPE
CKREYENPLDRRFHAQPNACPKCGPWVSLYKDGKLIAEKNEALELLIEEIKIGKIVAVKGVGGFHLICNATNEESVRTLR
KRKRRSEKPFAVMFKSLEQVEAYANPTELEKALLISPERPIVLIQKKKELAPSVSPGLKRVGAFLPYSPLHHLILNSLDF
PVVATSANISEEPIIKDNKEALEKLGELADLILVHNRDIKRRCDDSVVKVVSGVPTPIRRSRGYAPLPVEVPFELPKRVL
AVGGMLKNTFALGFKNQVILSQHIGDIENLNTLKVFEESVFDLMELYEFEPDVVVCDMHPRYETTRWAEEFSRKRGIPLI
KVQHHYAHMLSCMAENGIKEKVLGIAWDGTGYGEDGTLWGGEFLVADYTSYERAFNFKPVKLIGGEKAVKEPRRVALSLL
FDIFGEEALNLDLLPVKSFSERELKNLYLAWKKGINSPLSSSVGRLFDALASLLNLKQILSYEGQGAMMVEDLYDPLVKD
NYPYEIRGKEVDLRKAFLEVLKEKDKSLAASRFINTLAKVCEDIALMVGIERVCLSGGVMQNDPLVTKIKELLEKEKFKV
YTHQKVPPNDGGIALGQAVFGLSLV

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=755, Percent_Identity=40, Blast_Score=467, Evalue=1e-132,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 84763; Mature: 84632

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFKRLKLHLKGAVQGVGFRPFVYRIAKELGLKGFVINDSKGVYIEVEGEEERLKKFLFK
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCEEEEECCCHHHHHHHHHH
LNREKPPLARIYSQEIQFLEPVNYEDFVIRKSEEKGEKEVLVLPDIATCEDCLRELFTPE
HCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEECCHHHHHHHHHHHCCCC
DRRYMYPFINCTNCGPRFTIIERLPYDRKNTTMKVFEMCPECKREYENPLDRRFHAQPNA
CCEEEECEEEECCCCCCEEEHHHCCCCCCCCHHHHHHHCHHHHHHHCCHHHHHHCCCCCC
CPKCGPWVSLYKDGKLIAEKNEALELLIEEIKIGKIVAVKGVGGFHLICNATNEESVRTL
CCCCCCCCEEECCCCEEECHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCHHHHHHH
RKRKRRSEKPFAVMFKSLEQVEAYANPTELEKALLISPERPIVLIQKKKELAPSVSPGLK
HHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHEECCCCCEEEEECCHHCCCCCCHHHH
RVGAFLPYSPLHHLILNSLDFPVVATSANISEEPIIKDNKEALEKLGELADLILVHNRDI
HHHHCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCH
KRRCDDSVVKVVSGVPTPIRRSRGYAPLPVEVPFELPKRVLAVGGMLKNTFALGFKNQVI
HHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCH
LSQHIGDIENLNTLKVFEESVFDLMELYEFEPDVVVCDMHPRYETTRWAEEFSRKRGIPL
HHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCE
IKVQHHYAHMLSCMAENGIKEKVLGIAWDGTGYGEDGTLWGGEFLVADYTSYERAFNFKP
EEEHHHHHHHHHHHHHCCCHHHHEEEEECCCCCCCCCCEECCEEEEEECHHHHHHCCCCC
VKLIGGEKAVKEPRRVALSLLFDIFGEEALNLDLLPVKSFSERELKNLYLAWKKGINSPL
EEEECCCHHHHHHHHHHHHHHHHHHCCHHCCEEEEECCCCCHHHHHHHHHHHHHCCCCCH
SSSVGRLFDALASLLNLKQILSYEGQGAMMVEDLYDPLVKDNYPYEIRGKEVDLRKAFLE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHCCCCCEEECCCCCHHHHHHHH
VLKEKDKSLAASRFINTLAKVCEDIALMVGIERVCLSGGVMQNDPLVTKIKELLEKEKFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHEE
VYTHQKVPPNDGGIALGQAVFGLSLV
EEECCCCCCCCCCEEHHHHHHHHCCC
>Mature Secondary Structure 
PFKRLKLHLKGAVQGVGFRPFVYRIAKELGLKGFVINDSKGVYIEVEGEEERLKKFLFK
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCEEEEECCCHHHHHHHHHH
LNREKPPLARIYSQEIQFLEPVNYEDFVIRKSEEKGEKEVLVLPDIATCEDCLRELFTPE
HCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEECCHHHHHHHHHHHCCCC
DRRYMYPFINCTNCGPRFTIIERLPYDRKNTTMKVFEMCPECKREYENPLDRRFHAQPNA
CCEEEECEEEECCCCCCEEEHHHCCCCCCCCHHHHHHHCHHHHHHHCCHHHHHHCCCCCC
CPKCGPWVSLYKDGKLIAEKNEALELLIEEIKIGKIVAVKGVGGFHLICNATNEESVRTL
CCCCCCCCEEECCCCEEECHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCHHHHHHH
RKRKRRSEKPFAVMFKSLEQVEAYANPTELEKALLISPERPIVLIQKKKELAPSVSPGLK
HHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHEECCCCCEEEEECCHHCCCCCCHHHH
RVGAFLPYSPLHHLILNSLDFPVVATSANISEEPIIKDNKEALEKLGELADLILVHNRDI
HHHHCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCH
KRRCDDSVVKVVSGVPTPIRRSRGYAPLPVEVPFELPKRVLAVGGMLKNTFALGFKNQVI
HHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCH
LSQHIGDIENLNTLKVFEESVFDLMELYEFEPDVVVCDMHPRYETTRWAEEFSRKRGIPL
HHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCE
IKVQHHYAHMLSCMAENGIKEKVLGIAWDGTGYGEDGTLWGGEFLVADYTSYERAFNFKP
EEEHHHHHHHHHHHHHCCCHHHHEEEEECCCCCCCCCCEECCEEEEEECHHHHHHCCCCC
VKLIGGEKAVKEPRRVALSLLFDIFGEEALNLDLLPVKSFSERELKNLYLAWKKGINSPL
EEEECCCHHHHHHHHHHHHHHHHHHCCHHCCEEEEECCCCCHHHHHHHHHHHHHCCCCCH
SSSVGRLFDALASLLNLKQILSYEGQGAMMVEDLYDPLVKDNYPYEIRGKEVDLRKAFLE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHCCCCCEEECCCCCHHHHHHHH
VLKEKDKSLAASRFINTLAKVCEDIALMVGIERVCLSGGVMQNDPLVTKIKELLEKEKFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHEE
VYTHQKVPPNDGGIALGQAVFGLSLV
EEECCCCCCCCCCEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]