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The map label for this gene is mbhS1 [H]

Identifier: 15606077

GI number: 15606077

Start: 450916

End: 451977

Strand: Direct

Name: mbhS1 [H]

Synonym: aq_660

Alternate gene names: 15606077

Gene position: 450916-451977 (Clockwise)

Preceding gene: 15606076

Following gene: 15606078

Centisome position: 29.07

GC content: 48.87

Gene sequence:

>1062_bases
ATGGAAACTTTCTGGGAAGTTTTTAAAAGACACGGAGTAAGTAGAAGGGATTTCTTAAAGTTTGCAACTACGATAACGGG
CCTTATGGGCCTTGCCCCTTCCATGGTTCCCGAAGTCGTAAGGGCCATGGAGACTAAACCCAGAGTTCCCGTTTTATGGA
TACACGGACTCGAGTGCACGTGCTGTTCCGAGTCTTTCATACGTTCAGCTACTCCTCTGGCTTCCGATGTAGTTCTCTCC
ATGATTTCCCTGGAGTACGACGACACACTCTCTGCAGCTGCGGGAGAGGCGGTTGAAAAGCACAGGGAAAGAATCATAAA
GGAATACTGGGGCAACTACATACTCGCTGTTGAAGGAAACCCGCCCCTCGGCGAAGACGGCATGTACTGCATAATAGGCG
GAAGACCTTTTGTGGAAATACTTAAGGAAAGTGCGGAAGGGGCAAAGGCTGTAATAGCGTGGGGAAGTTGTGCTTCCTGG
GGATGCGTTCAGGCGGCAAAACCAAACCCCACGACTGCAGTTCCCATAGATAAAGTAATAAAGGATAAGCCAATAATAAA
GGTTCCCGGATGTCCTCCCATAGCCGAGGTTATGACCGGAGTAATTATGTATATGGTTCTATTCGACAGAATCCCTCCTT
TGGACTCTCAGGGAAGACCTAAGATGTTCTACGGAAACAGGATTCACGATACCTGTTACAGGAGATCCTTCTTCAACGCC
GGTCAATTCGTTGAGCAGTTTGACGACGAAGGGGCTAAGAAAGGTTGGTGCCTTTACAAGGTAGGATGCAGAGGACCCAC
GACTTACAACTCCTGTGGTAACATGAGGTGGTACAACGGACTCTCCTATCCCATCCAGTCGGGACACGGTTGTATAGGCT
GTGCGGAAAACAACTTCTGGGATAACGGACCCTTCTACGAAAGGATCGGTGGAATACCTGTTCCCGGAATAGAAAGCAAG
GCGGACAAGGTTGGAGCTATTGCGGCGGCAGCTGCTGCAGGCGGTGCAATAATTCACGGTATAGCTTCCAAGATAAGAAA
GTCAGGTGAAAAGGAAGAATAA

Upstream 100 bases:

>100_bases
GTTATTTACCTTCCTTAAGGAATTTTTATGATTTTAATAAGAAACTTGAATGAATTATCATTCACGAAAGTGTATACTAA
AAAAATAACAGGAGGTGGGT

Downstream 100 bases:

>100_bases
GGGAGGTGTTATAGATGAAGAGAGTTGTAGTTGATCCTGTAACAAGGATAGAAGGTCATTTGAGAATTGAGATAATGGTT
GACGAGGAAACAGGACAAGT

Product: hydrogenase small subunit

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase small subunit [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
METFWEVFKRHGVSRRDFLKFATTITGLMGLAPSMVPEVVRAMETKPRVPVLWIHGLECTCCSESFIRSATPLASDVVLS
MISLEYDDTLSAAAGEAVEKHRERIIKEYWGNYILAVEGNPPLGEDGMYCIIGGRPFVEILKESAEGAKAVIAWGSCASW
GCVQAAKPNPTTAVPIDKVIKDKPIIKVPGCPPIAEVMTGVIMYMVLFDRIPPLDSQGRPKMFYGNRIHDTCYRRSFFNA
GQFVEQFDDEGAKKGWCLYKVGCRGPTTYNSCGNMRWYNGLSYPIQSGHGCIGCAENNFWDNGPFYERIGGIPVPGIESK
ADKVGAIAAAAAAGGAIIHGIASKIRKSGEKEE

Sequences:

>Translated_353_residues
METFWEVFKRHGVSRRDFLKFATTITGLMGLAPSMVPEVVRAMETKPRVPVLWIHGLECTCCSESFIRSATPLASDVVLS
MISLEYDDTLSAAAGEAVEKHRERIIKEYWGNYILAVEGNPPLGEDGMYCIIGGRPFVEILKESAEGAKAVIAWGSCASW
GCVQAAKPNPTTAVPIDKVIKDKPIIKVPGCPPIAEVMTGVIMYMVLFDRIPPLDSQGRPKMFYGNRIHDTCYRRSFFNA
GQFVEQFDDEGAKKGWCLYKVGCRGPTTYNSCGNMRWYNGLSYPIQSGHGCIGCAENNFWDNGPFYERIGGIPVPGIESK
ADKVGAIAAAAAAGGAIIHGIASKIRKSGEKEE
>Mature_353_residues
METFWEVFKRHGVSRRDFLKFATTITGLMGLAPSMVPEVVRAMETKPRVPVLWIHGLECTCCSESFIRSATPLASDVVLS
MISLEYDDTLSAAAGEAVEKHRERIIKEYWGNYILAVEGNPPLGEDGMYCIIGGRPFVEILKESAEGAKAVIAWGSCASW
GCVQAAKPNPTTAVPIDKVIKDKPIIKVPGCPPIAEVMTGVIMYMVLFDRIPPLDSQGRPKMFYGNRIHDTCYRRSFFNA
GQFVEQFDDEGAKKGWCLYKVGCRGPTTYNSCGNMRWYNGLSYPIQSGHGCIGCAENNFWDNGPFYERIGGIPVPGIESK
ADKVGAIAAAAAAGGAIIHGIASKIRKSGEKEE

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=327, Percent_Identity=64.8318042813456, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1789371, Length=291, Percent_Identity=41.2371134020619, Blast_Score=254, Evalue=5e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 38619; Mature: 38619

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METFWEVFKRHGVSRRDFLKFATTITGLMGLAPSMVPEVVRAMETKPRVPVLWIHGLECT
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEECCCEE
CCSESFIRSATPLASDVVLSMISLEYDDTLSAAAGEAVEKHRERIIKEYWGNYILAVEGN
HHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
PPLGEDGMYCIIGGRPFVEILKESAEGAKAVIAWGSCASWGCVQAAKPNPTTAVPIDKVI
CCCCCCCCEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHH
KDKPIIKVPGCPPIAEVMTGVIMYMVLFDRIPPLDSQGRPKMFYGNRIHDTCYRRSFFNA
CCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHH
GQFVEQFDDEGAKKGWCLYKVGCRGPTTYNSCGNMRWYNGLSYPIQSGHGCIGCAENNFW
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCEECCCCCCCCCCCCCEEEECCCCCC
DNGPFYERIGGIPVPGIESKADKVGAIAAAAAAGGAIIHGIASKIRKSGEKEE
CCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
METFWEVFKRHGVSRRDFLKFATTITGLMGLAPSMVPEVVRAMETKPRVPVLWIHGLECT
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEECCCEE
CCSESFIRSATPLASDVVLSMISLEYDDTLSAAAGEAVEKHRERIIKEYWGNYILAVEGN
HHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
PPLGEDGMYCIIGGRPFVEILKESAEGAKAVIAWGSCASWGCVQAAKPNPTTAVPIDKVI
CCCCCCCCEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHH
KDKPIIKVPGCPPIAEVMTGVIMYMVLFDRIPPLDSQGRPKMFYGNRIHDTCYRRSFFNA
CCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHH
GQFVEQFDDEGAKKGWCLYKVGCRGPTTYNSCGNMRWYNGLSYPIQSGHGCIGCAENNFW
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCEECCCCCCCCCCCCCEEEECCCCCC
DNGPFYERIGGIPVPGIESKADKVGAIAAAAAAGGAIIHGIASKIRKSGEKEE
CCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1383192; 12948488; 2493816 [H]