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The map label for this gene is topA

Identifier: 15606076

GI number: 15606076

Start: 448782

End: 450404

Strand: Direct

Name: topA

Synonym: aq_657

Alternate gene names: 15606076

Gene position: 448782-450404 (Clockwise)

Preceding gene: 15606071

Following gene: 15606077

Centisome position: 28.93

GC content: 43.68

Gene sequence:

>1623_bases
ATGGAACTCTTTATAGTGGAGTCTCCAACGAAGGCAAAAACCATTCAGAAGTTCCTCGGAAAAGGCTTCTTAGTTAAAGC
CACACTGGGACACGTAAAGGATCTTCCCGAAAAGGAACTCGGCGTTGACCTCAGAACCCTGAAGGCAAAGTACGTATACA
AAAGGGGAAAGAAAAAACTCGTGGAACAACTCAAAAAATTGTCAAGAAGGTCAAGTATAGTCTATCTGGGAACGGACCCC
GACCGTGAAGGGGAAGCCATTGCCTACTTTTTAAAGAAAGATCTCGAAAAGGTAAATAAAAACATAAAGAGAGCTGTCTT
TTACGAAATAACGCCGGAAGCCATAAGGGAAAGCATAAGAAACGCGGGCGACGTGAACATGAACTTAGTTTACGCCCAGT
TTGCAAGGAGGATACTCGACAGACTCATAGGTTACTTAATATCCCCAATACTCTGGAAGGAGTTCAAGAACTACAAACTC
TCGGCGGGAAGAGTGCAGTCGCCTGCCCTCAGACTTATAGTGGAAAGGGAAAGGGAAATTCAGAACTTTAAAGTTAAGAA
GTATTACTACGTAAAGGCCTTACTCAGAAAGGGGAGTGAAGAGTTTTGGGCTATTTACGATTACCGCTACGAAAACCCCT
CGGACGCAAAAATCATAGCCAAAAAGCTGGAGAAGGGATATTTCAGCGTTTACAAAGTGGAAAAGAAAAAGGAAAAAGTG
TCACCTCCCAAGCCCTTTATAACCTCCGATCTCCAGTCAGAAGCAAATGCAAAGTTCGGATTTTCGAGTGAGAGGACACA
GAAGTTGGCTCAGGAACTCTACGAAAAGGGATACATAACCTATCCCAGAACGGACAGCTACAGGATGAACGAAAAAAAGG
CAAAGGAATTTATGAACTACATAGAAAAGAAGTACGGAAAGGAATACGTGGGAAGGTTGAGGAGGTTCAGGGAAAAGGCA
ACCGCGCAGGGTGCCCACGAGTGTATCAGACCAACAAGCCTGAGGGAAGAAATTCCTGAAAGGGAAGAATTGAGACTTCT
TTACGACTTGATTTTCAGGAGAACGATAGCGAGTCTTATGAAAGAAATGCTCCTGGAAAGGGAAAAGGTTACGGTAGAGG
CGATTACACCAGAACTCAAGCACCCTGTTTACCTCGTGGCAAAGGGATTGAAAATCGTCTTTGACGGATGGAGCAGAGTT
TACCCGAGTGAGATAACCGAAGAGAAACTTCCAGAACTTTACGAAGGGGATCTATTAGATCTCGTGAAAACGACACTTGA
GGAAAGGAAGACCCAGCCACCTCCGAGATACACGGAAGGAACTCTCATAAAGACCCTTGAGAAACTGGGAATAGGAAGAC
CTTCCACCTACGCAACTATAGTGAAAACACTGAAAGAGAGAGGGTACGTTGAAGTAAAGAAAAAAGCTTTAGTACCAACC
GAGATAGCCTTTCAGGTCGTTGAATTTTTAATGGAAAGATTTCCCACACTTATGGATTACAAGTTTACCGCCCAGATGGA
GGAAAAACTCGATCTCGTTGAAGAAGGAAAGCTCAATTGGAAGTCTGTAGTTTACGAGTTCATGGAAAAGATCTTCGGTA
AGGAGTTGGAAATGGTAAGATAG

Upstream 100 bases:

>100_bases
CTACTTCGTACTCCTCCCACCGAAAATCACCGTCCGAATAACGCTTTATCTTTACCTTCATACTCAAAATTTTATAGCTT
TAGAATTTATTATCCAGATT

Downstream 100 bases:

>100_bases
TGGTGGCCCAGGGCGGAGTCGAACCGCCGACACCCCGGTTTTCAGCCGGGTGCTCTACCACCTGAGCTACCGGGCCTTAC
GACAAAAATATATTATATAT

Product: topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MELFIVESPTKAKTIQKFLGKGFLVKATLGHVKDLPEKELGVDLRTLKAKYVYKRGKKKLVEQLKKLSRRSSIVYLGTDP
DREGEAIAYFLKKDLEKVNKNIKRAVFYEITPEAIRESIRNAGDVNMNLVYAQFARRILDRLIGYLISPILWKEFKNYKL
SAGRVQSPALRLIVEREREIQNFKVKKYYYVKALLRKGSEEFWAIYDYRYENPSDAKIIAKKLEKGYFSVYKVEKKKEKV
SPPKPFITSDLQSEANAKFGFSSERTQKLAQELYEKGYITYPRTDSYRMNEKKAKEFMNYIEKKYGKEYVGRLRRFREKA
TAQGAHECIRPTSLREEIPEREELRLLYDLIFRRTIASLMKEMLLEREKVTVEAITPELKHPVYLVAKGLKIVFDGWSRV
YPSEITEEKLPELYEGDLLDLVKTTLEERKTQPPPRYTEGTLIKTLEKLGIGRPSTYATIVKTLKERGYVEVKKKALVPT
EIAFQVVEFLMERFPTLMDYKFTAQMEEKLDLVEEGKLNWKSVVYEFMEKIFGKELEMVR

Sequences:

>Translated_540_residues
MELFIVESPTKAKTIQKFLGKGFLVKATLGHVKDLPEKELGVDLRTLKAKYVYKRGKKKLVEQLKKLSRRSSIVYLGTDP
DREGEAIAYFLKKDLEKVNKNIKRAVFYEITPEAIRESIRNAGDVNMNLVYAQFARRILDRLIGYLISPILWKEFKNYKL
SAGRVQSPALRLIVEREREIQNFKVKKYYYVKALLRKGSEEFWAIYDYRYENPSDAKIIAKKLEKGYFSVYKVEKKKEKV
SPPKPFITSDLQSEANAKFGFSSERTQKLAQELYEKGYITYPRTDSYRMNEKKAKEFMNYIEKKYGKEYVGRLRRFREKA
TAQGAHECIRPTSLREEIPEREELRLLYDLIFRRTIASLMKEMLLEREKVTVEAITPELKHPVYLVAKGLKIVFDGWSRV
YPSEITEEKLPELYEGDLLDLVKTTLEERKTQPPPRYTEGTLIKTLEKLGIGRPSTYATIVKTLKERGYVEVKKKALVPT
EIAFQVVEFLMERFPTLMDYKFTAQMEEKLDLVEEGKLNWKSVVYEFMEKIFGKELEMVR
>Mature_540_residues
MELFIVESPTKAKTIQKFLGKGFLVKATLGHVKDLPEKELGVDLRTLKAKYVYKRGKKKLVEQLKKLSRRSSIVYLGTDP
DREGEAIAYFLKKDLEKVNKNIKRAVFYEITPEAIRESIRNAGDVNMNLVYAQFARRILDRLIGYLISPILWKEFKNYKL
SAGRVQSPALRLIVEREREIQNFKVKKYYYVKALLRKGSEEFWAIYDYRYENPSDAKIIAKKLEKGYFSVYKVEKKKEKV
SPPKPFITSDLQSEANAKFGFSSERTQKLAQELYEKGYITYPRTDSYRMNEKKAKEFMNYIEKKYGKEYVGRLRRFREKA
TAQGAHECIRPTSLREEIPEREELRLLYDLIFRRTIASLMKEMLLEREKVTVEAITPELKHPVYLVAKGLKIVFDGWSRV
YPSEITEEKLPELYEGDLLDLVKTTLEERKTQPPPRYTEGTLIKTLEKLGIGRPSTYATIVKTLKERGYVEVKKKALVPT
EIAFQVVEFLMERFPTLMDYKFTAQMEEKLDLVEEGKLNWKSVVYEFMEKIFGKELEMVR

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Homo sapiens, GI4507635, Length=506, Percent_Identity=25.4940711462451, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI10835218, Length=484, Percent_Identity=26.2396694214876, Blast_Score=122, Evalue=1e-27,
Organism=Escherichia coli, GI1787529, Length=580, Percent_Identity=38.448275862069, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1788061, Length=628, Percent_Identity=26.4331210191083, Blast_Score=131, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI32563869, Length=543, Percent_Identity=25.2302025782689, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17555378, Length=455, Percent_Identity=26.3736263736264, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323263, Length=548, Percent_Identity=23.3576642335766, Blast_Score=104, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24585251, Length=579, Percent_Identity=25.5613126079447, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24640096, Length=501, Percent_Identity=23.9520958083832, Blast_Score=109, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOP1_AQUAE (O66893)

Other databases:

- EMBL:   AE000657
- PIR:   A70358
- RefSeq:   NP_213453.1
- ProteinModelPortal:   O66893
- SMR:   O66893
- GeneID:   1193955
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_657
- NMPDR:   fig|224324.1.peg.468
- HOGENOM:   HBG512935
- OMA:   WICEREE
- ProtClustDB:   CLSK2748336
- BioCyc:   AAEO224324:AQ_657-MONOMER
- BRENDA:   5.99.1.2
- GO:   GO:0005694
- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013826
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005733
- InterPro:   IPR006171
- Gene3D:   G3DSA:1.10.460.10
- Gene3D:   G3DSA:1.10.290.10
- PANTHER:   PTHR11390
- PRINTS:   PR00417
- SMART:   SM00437
- SMART:   SM00436
- SMART:   SM00493
- TIGRFAMs:   TIGR01051

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; SSF56712 Topo_IA_core

EC number: =5.99.1.2

Molecular weight: Translated: 63428; Mature: 63428

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: ACT_SITE 281-281

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELFIVESPTKAKTIQKFLGKGFLVKATLGHVKDLPEKELGVDLRTLKAKYVYKRGKKKL
CEEEEECCCHHHHHHHHHHCCCEEEEHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHH
VEQLKKLSRRSSIVYLGTDPDREGEAIAYFLKKDLEKVNKNIKRAVFYEITPEAIRESIR
HHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHH
NAGDVNMNLVYAQFARRILDRLIGYLISPILWKEFKNYKLSAGRVQSPALRLIVEREREI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
QNFKVKKYYYVKALLRKGSEEFWAIYDYRYENPSDAKIIAKKLEKGYFSVYKVEKKKEKV
HHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
SPPKPFITSDLQSEANAKFGFSSERTQKLAQELYEKGYITYPRTDSYRMNEKKAKEFMNY
CCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHHH
IEKKYGKEYVGRLRRFREKATAQGAHECIRPTSLREEIPEREELRLLYDLIFRRTIASLM
HHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHH
KEMLLEREKVTVEAITPELKHPVYLVAKGLKIVFDGWSRVYPSEITEEKLPELYEGDLLD
HHHHHHHHHHHHHHHCHHHCCCHHHHHCCCHHEECCHHHCCCHHCCHHHCHHHHCCHHHH
LVKTTLEERKTQPPPRYTEGTLIKTLEKLGIGRPSTYATIVKTLKERGYVEVKKKALVPT
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEHHHHCCHH
EIAFQVVEFLMERFPTLMDYKFTAQMEEKLDLVEEGKLNWKSVVYEFMEKIFGKELEMVR
HHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHCC
>Mature Secondary Structure
MELFIVESPTKAKTIQKFLGKGFLVKATLGHVKDLPEKELGVDLRTLKAKYVYKRGKKKL
CEEEEECCCHHHHHHHHHHCCCEEEEHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHH
VEQLKKLSRRSSIVYLGTDPDREGEAIAYFLKKDLEKVNKNIKRAVFYEITPEAIRESIR
HHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHH
NAGDVNMNLVYAQFARRILDRLIGYLISPILWKEFKNYKLSAGRVQSPALRLIVEREREI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
QNFKVKKYYYVKALLRKGSEEFWAIYDYRYENPSDAKIIAKKLEKGYFSVYKVEKKKEKV
HHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
SPPKPFITSDLQSEANAKFGFSSERTQKLAQELYEKGYITYPRTDSYRMNEKKAKEFMNY
CCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHHH
IEKKYGKEYVGRLRRFREKATAQGAHECIRPTSLREEIPEREELRLLYDLIFRRTIASLM
HHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHH
KEMLLEREKVTVEAITPELKHPVYLVAKGLKIVFDGWSRVYPSEITEEKLPELYEGDLLD
HHHHHHHHHHHHHHHCHHHCCCHHHHHCCCHHEECCHHHCCCHHCCHHHCHHHHCCHHHH
LVKTTLEERKTQPPPRYTEGTLIKTLEKLGIGRPSTYATIVKTLKERGYVEVKKKALVPT
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEHHHHCCHH
EIAFQVVEFLMERFPTLMDYKFTAQMEEKLDLVEEGKLNWKSVVYEFMEKIFGKELEMVR
HHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320