Click here to switch to the map view.

The map label for this gene is cofH2 [H]

Identifier: 15606071

GI number: 15606071

Start: 444064

End: 445179

Strand: Direct

Name: cofH2 [H]

Synonym: aq_648

Alternate gene names: 15606071

Gene position: 444064-445179 (Clockwise)

Preceding gene: 15606070

Following gene: 15606076

Centisome position: 28.62

GC content: 45.16

Gene sequence:

>1116_bases
ATGGAACTCCAGGTGGAGACGGTCTTTCAAACGGTTAGAGATAAAGAGTTTTTAAGGATAGCGGATAAAGTTTTAAACGA
AAAGCGTATAAATCAGGAAGAAGCTTTATACCTTTACAGGAGCAATGAGCTTACCGCCCTCGGACTGCTTGCGGAATACA
TAAACAGGAAGAAAAACGGAATGTATGCCTACTTTATAGTGAACAGACAGATAAATCCCACAAATGTATGCATGTATCAG
TGCGACTTTTGCGCCTTCGGAGTTAAGAAGTCGGATCCCAAAGCCTACGAGATGAGTATGGAAGAAATACTGAAAAAAGT
GGAAGAGCTTTACCTCATGGGAGGCAGGGAAGTTCACATAGTAGGAGGGATACCTTCTTACTGGGATTACGATAAGTACG
TAAGTATAGTAAGGGAAATTAAAAAGCGTTTTCCCGAGATAATAATAAAGGCTTGGACCGCGGTTGAGATACACCACATG
GCGAAAATCTCCGGAAAGTCCTACGAAGAAGTTCTGAGTGAATTAAAAGAAGCGGGACTTGACGCTCTTCCGGGGGGCGG
TGCGGAAATATTCTCGGAAAGGGTGAGGAAGATAATAGCTCCTTACAAGGCAAACGCGGAAGAGTACTTAGAAGTTCACA
GAGTTGCTCACAAACTCGGGATTCCCAGTAACGCAACGATGCTTTACGGACATGTGGAAACACTTGAGGAAAGGGTAGAA
CACATGGAGATGCTGAGAAGACTTCAGGACGAAACGGGAGGCTTTCAGGTGTTCATACCTCTCGCTTACTGGCCTGAAGG
GACGAGGCTCGGCGGAACGAGAACCTCTTCCGTAGACGACCTGAAAACTATAGCTATATCGAGGATTTACCTTGATAACT
TTGATCATATAAAAGCTTACTGGGTTACCTTGGGTGAAAAAGTAGCACAGGTTGCCCTGAACTTCGGTGCGGATGATCTG
GACGGCACGATACAGGAGGAAAAAATAGTACACGCTGCAGGAACCAAATCCGCTTACGGGCACTCCGTGGACAAACTCGT
GAACCTCATAAAGAAAGCGGGTAAAACTCCCGCTGAAAGGGATACCTTCTACAGAGTTGTCAGAGTTTACGATTAA

Upstream 100 bases:

>100_bases
AGGAATCTCGCGCTCTATCCGCCTGAGCTACGGGCACTACCACTAAATAAATATTATATATCCTTTCGCCTACAGAAAAA
GGAATTAAAATTTAAGGATT

Downstream 100 bases:

>100_bases
GAATTTTCTCAATTTCTTCTTTTTTTACCTTCCCTTCCCTTAATATCTTTACACCTTCTGTTAGGTCCAGTATAGTTGAT
GGTTCTCCCTTTATAACTCC

Product: hypothetical protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 2 [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MELQVETVFQTVRDKEFLRIADKVLNEKRINQEEALYLYRSNELTALGLLAEYINRKKNGMYAYFIVNRQINPTNVCMYQ
CDFCAFGVKKSDPKAYEMSMEEILKKVEELYLMGGREVHIVGGIPSYWDYDKYVSIVREIKKRFPEIIIKAWTAVEIHHM
AKISGKSYEEVLSELKEAGLDALPGGGAEIFSERVRKIIAPYKANAEEYLEVHRVAHKLGIPSNATMLYGHVETLEERVE
HMEMLRRLQDETGGFQVFIPLAYWPEGTRLGGTRTSSVDDLKTIAISRIYLDNFDHIKAYWVTLGEKVAQVALNFGADDL
DGTIQEEKIVHAAGTKSAYGHSVDKLVNLIKKAGKTPAERDTFYRVVRVYD

Sequences:

>Translated_371_residues
MELQVETVFQTVRDKEFLRIADKVLNEKRINQEEALYLYRSNELTALGLLAEYINRKKNGMYAYFIVNRQINPTNVCMYQ
CDFCAFGVKKSDPKAYEMSMEEILKKVEELYLMGGREVHIVGGIPSYWDYDKYVSIVREIKKRFPEIIIKAWTAVEIHHM
AKISGKSYEEVLSELKEAGLDALPGGGAEIFSERVRKIIAPYKANAEEYLEVHRVAHKLGIPSNATMLYGHVETLEERVE
HMEMLRRLQDETGGFQVFIPLAYWPEGTRLGGTRTSSVDDLKTIAISRIYLDNFDHIKAYWVTLGEKVAQVALNFGADDL
DGTIQEEKIVHAAGTKSAYGHSVDKLVNLIKKAGKTPAERDTFYRVVRVYD
>Mature_371_residues
MELQVETVFQTVRDKEFLRIADKVLNEKRINQEEALYLYRSNELTALGLLAEYINRKKNGMYAYFIVNRQINPTNVCMYQ
CDFCAFGVKKSDPKAYEMSMEEILKKVEELYLMGGREVHIVGGIPSYWDYDKYVSIVREIKKRFPEIIIKAWTAVEIHHM
AKISGKSYEEVLSELKEAGLDALPGGGAEIFSERVRKIIAPYKANAEEYLEVHRVAHKLGIPSNATMLYGHVETLEERVE
HMEMLRRLQDETGGFQVFIPLAYWPEGTRLGGTRTSSVDDLKTIAISRIYLDNFDHIKAYWVTLGEKVAQVALNFGADDL
DGTIQEEKIVHAAGTKSAYGHSVDKLVNLIKKAGKTPAERDTFYRVVRVYD

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 42452; Mature: 42452

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELQVETVFQTVRDKEFLRIADKVLNEKRINQEEALYLYRSNELTALGLLAEYINRKKNG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEECCCCHHHHHHHHHHHHCCCC
MYAYFIVNRQINPTNVCMYQCDFCAFGVKKSDPKAYEMSMEEILKKVEELYLMGGREVHI
EEEEEEEECCCCCCEEEEEEECHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
VGGIPSYWDYDKYVSIVREIKKRFPEIIIKAWTAVEIHHMAKISGKSYEEVLSELKEAGL
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
DALPGGGAEIFSERVRKIIAPYKANAEEYLEVHRVAHKLGIPSNATMLYGHVETLEERVE
CCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHH
HMEMLRRLQDETGGFQVFIPLAYWPEGTRLGGTRTSSVDDLKTIAISRIYLDNFDHIKAY
HHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHH
WVTLGEKVAQVALNFGADDLDGTIQEEKIVHAAGTKSAYGHSVDKLVNLIKKAGKTPAER
HHHHHHHHHHHHHHCCCCCCCCCHHHHHEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCH
DTFYRVVRVYD
HHHHHEEEECC
>Mature Secondary Structure
MELQVETVFQTVRDKEFLRIADKVLNEKRINQEEALYLYRSNELTALGLLAEYINRKKNG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEECCCCHHHHHHHHHHHHCCCC
MYAYFIVNRQINPTNVCMYQCDFCAFGVKKSDPKAYEMSMEEILKKVEELYLMGGREVHI
EEEEEEEECCCCCCEEEEEEECHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
VGGIPSYWDYDKYVSIVREIKKRFPEIIIKAWTAVEIHHMAKISGKSYEEVLSELKEAGL
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
DALPGGGAEIFSERVRKIIAPYKANAEEYLEVHRVAHKLGIPSNATMLYGHVETLEERVE
CCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHH
HMEMLRRLQDETGGFQVFIPLAYWPEGTRLGGTRTSSVDDLKTIAISRIYLDNFDHIKAY
HHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHH
WVTLGEKVAQVALNFGADDLDGTIQEEKIVHAAGTKSAYGHSVDKLVNLIKKAGKTPAER
HHHHHHHHHHHHHHCCCCCCCCCHHHHHEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCH
DTFYRVVRVYD
HHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA