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The map label for this gene is ligA

Identifier: 15606063

GI number: 15606063

Start: 433166

End: 435328

Strand: Direct

Name: ligA

Synonym: aq_633

Alternate gene names: 15606063

Gene position: 433166-435328 (Clockwise)

Preceding gene: 15606058

Following gene: 15606066

Centisome position: 27.92

GC content: 44.38

Gene sequence:

>2163_bases
ATGTTTACCCCAGAAAGGGAAAAGGAACTTCAGGAAAAAACACGGGAACTCCTGAGAAAGATTAAGGACGTAAAAGTCCT
GAGCTTTGAGGAAGCTAAAAAACTCGCGGAAGACCTCAGAGAAGTAATCAGGTATCACGATTACAAGTACTACGTCGAGG
CAAACCCAGTAATTCCCGATTACGATTACGACAGACTCTTCAGGGCTTTAAAAGAAATAGAGAAAAAATACCCCGAGCTT
ATCACTCCCGATTCTCCTACTCAGAGAGTGGCGAGCGAAATAAGCGGAGAGTTTCCCACGGTTAAACACTACACCCCTAT
GCTCTCTCTGGACAACGCCTACTCGGAAGATGAACTCAGAGAGTTTGACAGGAGGGTAAGACAGATTACGGGATTGGAAG
TAGTAGAGTACGCGGTAGAGCCAAAACTGGACGGTGCGGGTATAGCTCTTGTTTATGAAAACGATTTGTTCGTCAGGGGG
GCTACGAGGGGCGACGGTGAGTACGGGGAAGACATAACGAATAACCTCAAAACTATTAAAACAATACCACTAAAGGCGGA
ATTTTCCCGTTTCGGAATCAAACTTGCGGAAATCAGGGGAGAAGTAGTTATAAGGAAAGATGAATTTCAAAAATTAAATA
AAGAAAGAATGGAAGAAGGATTGCCTCCGTTTGCCAATCCCAGGAATGCGGCTGCAGGCTCCATTAGGCAGAAGGATCCT
AAGGAAGTTGCTAAGAGAAATCTGGAAGCTATCGTATACCACCTCTCTTACGTAGAACCCCCTGAGACCGAACCGCCAAC
CCACTACGAATCTTTGAAAATGCTCCATACACTCGGTTTCAAGACGCTCTTTAAGGACACAAAAGTCTGCAAGGGCATAG
ACGAAGTTATAGAGTACTGCAAGGAGTGGGAGAAAAAGAGGGACTCTTACCCTTACGAGATAGACGGAATGGTCGTTAAG
GTGAACGACAGAAGGTTGTGGAAGGTTCTCGGTTACACTTCCCACCATCCGAGGTGGGCTATAGCGTATAAGTTCAAGCC
AAGGAGAGCGGTAACAAAACTCGTTGACGTTGTCTTTCAGGTGGGAAGGACGGGAACGATTACACCTGTGGGTAAGCTCG
AGCCTGTAGAGCTGGGAGGCGTTACCGTTTCTTCTGTATCTCTATTTAACGAAGACTTTATAAGGGAAAAGGACATCAGG
ATAGGTGACTGGGTGGTAGTTGAGAGGGCTGGAGACGTTATACCTTACGTGGTTGAAGTCTTAAAGGAAAAGAGAACGGG
TGAAGAGAAACCCGTGGAATTTCCAAAGTACTGTCCTTCCTGCGGTTCTGAGCTCGTAAAACTTCCCGAGGAAGTAGCTA
TAAGGTGTATAAACATATCCTGCCCCGCTCAGAGCGTTTTGAGGATTAAACACTGGGCGAGCAGAGACGCAATGGACATA
AGAGGACTGGGAGACGCCACAATAAAACTTCTATTCAACAGGGGACTCGCAAAGGACGTGGGAGACCTTTACTACCTAAA
GTTGACGGACATTTTAAAGCTTCCTGGATTTGGAGAAAAATCCGCTATGAACTTACTGAAGGCTATTGAGGAGAGCAAAA
ATAGACCTCTTGATAGAGTCCTTTACGGACTCGGTATAAGGTACGTGGGACAAACTACAGCAAAAAAAATAGCCGAAATT
ATAAACAGTGTATGGGATTTAAAAGACATTCCCCTTGAGAAGTTGATGAGATTGGAAGGAATAGGGTACAAGGTAGCAAG
GAGTATAAAGGAGTTCTTCAACATACCTCAAAACCTTGAAGTTCTTAAAAAACTGGAAAAGGCGGGTGTAAACCTCGCCA
AGAAAGTCAAAGAAAAAGTTGCAGACGTTTTGAAAGGAAAAACTTTCGTTTTCACGGGAACGCTCGATTGTTGTTCGAGG
GAAAAGGCGGGGGAGATTGTTGAGATGCTCGGCGGAAAGTTCTCAAACTCTGTGACTTCCAAAACAGATTACCTAGTGGT
GGGTAAAGATCCCGGAGCTACAAAACTCAGTAAAGCTAAGAAGTACGGAGTAAAGACTATAACCGAAGAGGAATTCGTAA
ACATGATAAAAGATTACGTGGATTTGGAGAAAATAAAGAAGGAGGATAAGAAGGAAAAACCGAAAATAGGCAGACTATTT
TGA

Upstream 100 bases:

>100_bases
CTGTACCCTCTTCCCGTACTTTTCCTTGAAGTAGTCCTTAAGGGAGTAGTAGAGTCTTTGAGTCTTCACTTGAGCCATGA
TTTTATTAATTTAATTCTGT

Downstream 100 bases:

>100_bases
AACCTTTTTCTTTACTTCCTTGTTTACTCCGAAGAGCTTTTTCAGTTCCTCCTCTATCTTATCCCTTATCCTTCTGAGTT
CGGGTATGGAAAGGTTTTTG

Product: DNA ligase (NAD dependent)

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 720; Mature: 720

Protein sequence:

>720_residues
MFTPEREKELQEKTRELLRKIKDVKVLSFEEAKKLAEDLREVIRYHDYKYYVEANPVIPDYDYDRLFRALKEIEKKYPEL
ITPDSPTQRVASEISGEFPTVKHYTPMLSLDNAYSEDELREFDRRVRQITGLEVVEYAVEPKLDGAGIALVYENDLFVRG
ATRGDGEYGEDITNNLKTIKTIPLKAEFSRFGIKLAEIRGEVVIRKDEFQKLNKERMEEGLPPFANPRNAAAGSIRQKDP
KEVAKRNLEAIVYHLSYVEPPETEPPTHYESLKMLHTLGFKTLFKDTKVCKGIDEVIEYCKEWEKKRDSYPYEIDGMVVK
VNDRRLWKVLGYTSHHPRWAIAYKFKPRRAVTKLVDVVFQVGRTGTITPVGKLEPVELGGVTVSSVSLFNEDFIREKDIR
IGDWVVVERAGDVIPYVVEVLKEKRTGEEKPVEFPKYCPSCGSELVKLPEEVAIRCINISCPAQSVLRIKHWASRDAMDI
RGLGDATIKLLFNRGLAKDVGDLYYLKLTDILKLPGFGEKSAMNLLKAIEESKNRPLDRVLYGLGIRYVGQTTAKKIAEI
INSVWDLKDIPLEKLMRLEGIGYKVARSIKEFFNIPQNLEVLKKLEKAGVNLAKKVKEKVADVLKGKTFVFTGTLDCCSR
EKAGEIVEMLGGKFSNSVTSKTDYLVVGKDPGATKLSKAKKYGVKTITEEEFVNMIKDYVDLEKIKKEDKKEKPKIGRLF

Sequences:

>Translated_720_residues
MFTPEREKELQEKTRELLRKIKDVKVLSFEEAKKLAEDLREVIRYHDYKYYVEANPVIPDYDYDRLFRALKEIEKKYPEL
ITPDSPTQRVASEISGEFPTVKHYTPMLSLDNAYSEDELREFDRRVRQITGLEVVEYAVEPKLDGAGIALVYENDLFVRG
ATRGDGEYGEDITNNLKTIKTIPLKAEFSRFGIKLAEIRGEVVIRKDEFQKLNKERMEEGLPPFANPRNAAAGSIRQKDP
KEVAKRNLEAIVYHLSYVEPPETEPPTHYESLKMLHTLGFKTLFKDTKVCKGIDEVIEYCKEWEKKRDSYPYEIDGMVVK
VNDRRLWKVLGYTSHHPRWAIAYKFKPRRAVTKLVDVVFQVGRTGTITPVGKLEPVELGGVTVSSVSLFNEDFIREKDIR
IGDWVVVERAGDVIPYVVEVLKEKRTGEEKPVEFPKYCPSCGSELVKLPEEVAIRCINISCPAQSVLRIKHWASRDAMDI
RGLGDATIKLLFNRGLAKDVGDLYYLKLTDILKLPGFGEKSAMNLLKAIEESKNRPLDRVLYGLGIRYVGQTTAKKIAEI
INSVWDLKDIPLEKLMRLEGIGYKVARSIKEFFNIPQNLEVLKKLEKAGVNLAKKVKEKVADVLKGKTFVFTGTLDCCSR
EKAGEIVEMLGGKFSNSVTSKTDYLVVGKDPGATKLSKAKKYGVKTITEEEFVNMIKDYVDLEKIKKEDKKEKPKIGRLF
>Mature_720_residues
MFTPEREKELQEKTRELLRKIKDVKVLSFEEAKKLAEDLREVIRYHDYKYYVEANPVIPDYDYDRLFRALKEIEKKYPEL
ITPDSPTQRVASEISGEFPTVKHYTPMLSLDNAYSEDELREFDRRVRQITGLEVVEYAVEPKLDGAGIALVYENDLFVRG
ATRGDGEYGEDITNNLKTIKTIPLKAEFSRFGIKLAEIRGEVVIRKDEFQKLNKERMEEGLPPFANPRNAAAGSIRQKDP
KEVAKRNLEAIVYHLSYVEPPETEPPTHYESLKMLHTLGFKTLFKDTKVCKGIDEVIEYCKEWEKKRDSYPYEIDGMVVK
VNDRRLWKVLGYTSHHPRWAIAYKFKPRRAVTKLVDVVFQVGRTGTITPVGKLEPVELGGVTVSSVSLFNEDFIREKDIR
IGDWVVVERAGDVIPYVVEVLKEKRTGEEKPVEFPKYCPSCGSELVKLPEEVAIRCINISCPAQSVLRIKHWASRDAMDI
RGLGDATIKLLFNRGLAKDVGDLYYLKLTDILKLPGFGEKSAMNLLKAIEESKNRPLDRVLYGLGIRYVGQTTAKKIAEI
INSVWDLKDIPLEKLMRLEGIGYKVARSIKEFFNIPQNLEVLKKLEKAGVNLAKKVKEKVADVLKGKTFVFTGTLDCCSR
EKAGEIVEMLGGKFSNSVTSKTDYLVVGKDPGATKLSKAKKYGVKTITEEEFVNMIKDYVDLEKIKKEDKKEKPKIGRLF

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=85, Percent_Identity=37.6470588235294, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1788750, Length=664, Percent_Identity=40.5120481927711, Blast_Score=488, Evalue=1e-139,
Organism=Escherichia coli, GI87082305, Length=515, Percent_Identity=22.7184466019417, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_AQUAE (O66880)

Other databases:

- EMBL:   AE000657
- PIR:   D70356
- RefSeq:   NP_213440.1
- ProteinModelPortal:   O66880
- GeneID:   1193942
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_633
- NMPDR:   fig|224324.1.peg.455
- HOGENOM:   HBG620317
- OMA:   FHELKAL
- PhylomeDB:   O66880
- ProtClustDB:   CLSK2299454
- BioCyc:   AAEO224324:AQ_633-MONOMER
- BRENDA:   6.5.1.2
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 82319; Mature: 82319

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 142-142 BINDING 140-140 BINDING 163-163 BINDING 201-201 BINDING 320-320 BINDING 344-344

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTPEREKELQEKTRELLRKIKDVKVLSFEEAKKLAEDLREVIRYHDYKYYVEANPVIPD
CCCCHHHHHHHHHHHHHHHHHHHCHHCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCC
YDYDRLFRALKEIEKKYPELITPDSPTQRVASEISGEFPTVKHYTPMLSLDNAYSEDELR
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHHCCCCEECCCCCCHHHHH
EFDRRVRQITGLEVVEYAVEPKLDGAGIALVYENDLFVRGATRGDGEYGEDITNNLKTIK
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEECCCCCCCCCHHHHCCCHHEE
TIPLKAEFSRFGIKLAEIRGEVVIRKDEFQKLNKERMEEGLPPFANPRNAAAGSIRQKDP
ECCCHHHHHHHCEEEEEECCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCH
KEVAKRNLEAIVYHLSYVEPPETEPPTHYESLKMLHTLGFKTLFKDTKVCKGIDEVIEYC
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
KEWEKKRDSYPYEIDGMVVKVNDRRLWKVLGYTSHHPRWAIAYKFKPRRAVTKLVDVVFQ
HHHHHHCCCCCEEECCEEEEECCCHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHH
VGRTGTITPVGKLEPVELGGVTVSSVSLFNEDFIREKDIRIGDWVVVERAGDVIPYVVEV
CCCCCCCCCCCCCCCEEECCEEEHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHH
LKEKRTGEEKPVEFPKYCPSCGSELVKLPEEVAIRCINISCPAQSVLRIKHWASRDAMDI
HHHHHCCCCCCCCCHHCCHHHHHHHHHCCHHHEEEEEECCCCHHHHHHHHHHCCCCCCCC
RGLGDATIKLLFNRGLAKDVGDLYYLKLTDILKLPGFGEKSAMNLLKAIEESKNRPLDRV
CCCCHHHHHHHHHCCCHHHHCCEEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHH
LYGLGIRYVGQTTAKKIAEIINSVWDLKDIPLEKLMRLEGIGYKVARSIKEFFNIPQNLE
HHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCHHH
VLKKLEKAGVNLAKKVKEKVADVLKGKTFVFTGTLDCCSREKAGEIVEMLGGKFSNSVTS
HHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCC
KTDYLVVGKDPGATKLSKAKKYGVKTITEEEFVNMIKDYVDLEKIKKEDKKEKPKIGRLF
CCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MFTPEREKELQEKTRELLRKIKDVKVLSFEEAKKLAEDLREVIRYHDYKYYVEANPVIPD
CCCCHHHHHHHHHHHHHHHHHHHCHHCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCC
YDYDRLFRALKEIEKKYPELITPDSPTQRVASEISGEFPTVKHYTPMLSLDNAYSEDELR
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHHCCCCEECCCCCCHHHHH
EFDRRVRQITGLEVVEYAVEPKLDGAGIALVYENDLFVRGATRGDGEYGEDITNNLKTIK
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEECCCCCCCCCHHHHCCCHHEE
TIPLKAEFSRFGIKLAEIRGEVVIRKDEFQKLNKERMEEGLPPFANPRNAAAGSIRQKDP
ECCCHHHHHHHCEEEEEECCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCH
KEVAKRNLEAIVYHLSYVEPPETEPPTHYESLKMLHTLGFKTLFKDTKVCKGIDEVIEYC
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
KEWEKKRDSYPYEIDGMVVKVNDRRLWKVLGYTSHHPRWAIAYKFKPRRAVTKLVDVVFQ
HHHHHHCCCCCEEECCEEEEECCCHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHH
VGRTGTITPVGKLEPVELGGVTVSSVSLFNEDFIREKDIRIGDWVVVERAGDVIPYVVEV
CCCCCCCCCCCCCCCEEECCEEEHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHH
LKEKRTGEEKPVEFPKYCPSCGSELVKLPEEVAIRCINISCPAQSVLRIKHWASRDAMDI
HHHHHCCCCCCCCCHHCCHHHHHHHHHCCHHHEEEEEECCCCHHHHHHHHHHCCCCCCCC
RGLGDATIKLLFNRGLAKDVGDLYYLKLTDILKLPGFGEKSAMNLLKAIEESKNRPLDRV
CCCCHHHHHHHHHCCCHHHHCCEEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHH
LYGLGIRYVGQTTAKKIAEIINSVWDLKDIPLEKLMRLEGIGYKVARSIKEFFNIPQNLE
HHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCHHH
VLKKLEKAGVNLAKKVKEKVADVLKGKTFVFTGTLDCCSREKAGEIVEMLGGKFSNSVTS
HHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCC
KTDYLVVGKDPGATKLSKAKKYGVKTITEEEFVNMIKDYVDLEKIKKEDKKEKPKIGRLF
CCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320