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The map label for this gene is mrcA

Identifier: 15606057

GI number: 15606057

Start: 423373

End: 425553

Strand: Direct

Name: mrcA

Synonym: aq_624

Alternate gene names: 15606057

Gene position: 423373-425553 (Clockwise)

Preceding gene: 15606055

Following gene: 15606058

Centisome position: 27.29

GC content: 44.34

Gene sequence:

>2181_bases
ATGAAAAAGTTAGTAATAGGTATTTTAGGAATAGTTATAGCCTTATTTGTCGGACTGCTTGTCTTCCTCATACCCATCTA
CAAAAACCTTCCCGATCCGAAACTCTTGGAGAGCTGGACACCGCCTCAAGCCTCGGAGGTTTACGATGCAAAGGGAAGGT
TGTACGGGACGATAGGCATCCAGAAGAGGTTTTACGTGAGTATAGACAAAATTCCCGAACACGTTATAAACGCCTTCGTT
GCGACTGAGGACAGAAACTTCTGGCATCACTTCGGGATTGACCCTGTGGCAATAGTAAGGGCTGCAATTGTGAACTACAG
AGCCGGCAGGATAGTTCAGGGAGGTAGTACGATAACCCAGCAACTCGCTAAAAACTTATTCCTAACTAGAGAAAGGACAC
TGGAGAGAAAGATAAAGGAGGCACTCCTTGCCATAAAGATTGAGAGAACCTTTGACAAAAAGAAGATAATGGAATTGTAT
TTAAACCAGATATATCTAGGGAGCGGAGCTTACGGCGTTGAAGCGGCAGCTCAGGTATACTTCGGAAAACACGTTTGGGA
ACTTTCCTTAGATGAGGCAGCGCTCCTAGCAGCTCTGCCAAAAGCCCCCGCAAAGTACAACCCCTTTTACCACCCCGAGA
GGGCACTCCAGAGAAGAAATCTCGTACTGAAACGGATGCTAGAAGAAGGTTATATCACTCCAGAGCAGTACGAGGAGGCG
GTGAATAAACCCCTGACGGTAAAAAAAGAAAACAAGTACAAATTCTCCGATTACTTCCTCGATATGGTGAAGAGTTACGT
TTTTAACAAGTATGGGGAAATTGCATATAAGGGCAGGTTAAAGATATACACAACGATAGATTTGGACTACCAGAAAATCG
CTCAAAAATCGCTTGAGGAAGGTTTGAAGAGAGTAGCAAAGATTATAGGACTTCCTTTCCTTCCAAAGTCCGAAGAAGAT
ATGGAGCTCGCTTATGAGAAAGAAGCACAGTTAAAGAGATTAAAAAGAGGAAAGATCTACGTAGCTAAGATCTTGAAGTA
CGACGGAAATTTCATGAAAGTAGAAATCCACGGCAAAAAATTAAAGGGAGAAATAAAAGGGCTTAACACCGAAGGACATA
AGTATGTTTTCGTGAAATACCTCGGAGGAAATAGGGCGGAGATAATTCCTGACCTTGAGGGGAGTTTAGTCAGCATAGAC
GTAAAAACTGGAGAAATAAAGGCTATAGTGGGAGGAAGGAGTTACGCCTACTCTCAGTTCAACAGAGCTGTAAAAGCCCT
AAGACAACCCGGTTCAGCTATAAAGCCCGTGATATACCTGTCCGCACTTCTGAAGGGAATGACTCAAATTTCCACGATTG
ACGCTTCTTCCAAACCGTACTACGACCCCTCAAAGGGCGAAGACTGGATTCCCAAAAACTATGACGAAAAAGAATACGGA
AACGTTACCCTCAGGTATGCTCTTGCACACAGTATAAATACCGCGGCAGTAAACTTGCTGGACAAAGTGGGTTTTGAACT
AGTCCTGGAAGTTGGCAAGAAAGTAGGATTAGACAATTTAAAACCTTACTACTCTTTAGCTCTGGGAACGGTAGAGGTCA
CGCCCCTGCAACTCACTGCGGCTTATCAAGTCTTTGCAAACCTCGGAACGGAGTGTAAACCTTTCTTTATAAAGAAGATA
GTGGATGAAAACGGAGAAGTGCTAGAAGAAAACGTACCAGAGTGTGAGGAAGTCCTTCCAAAGCCTGAAACGAGAGTACC
CGTGGATATGCTCAGAGCCGTGGTGCTGGAAGGTACAGCGAGGAGGGCGAGTGTACTGGACAGGATAGTAGCCGGAAAAA
CGGGAACTACCGACGATTTTCAGGATGCATGGTTTGTAGGCTTTTCTCCATACATAGTGACGGGTGTATGGGTGGGGTAC
GACGTGAAAAAGTCTCTCGGAAAGCATATGTCGGGTTCAAGGGTAGCACTTCCAATATGGATAGACTACATGAAAGTTGT
TACAAGGATGTATCCAAACGAGGACTTTGAACTCCCTCCTGAAAATATAGTAGTAAATATAAACCCTAAAGACCTTGTTC
TCGCGGATGAGACTTGCGAAGGTGTCCCTATGGTTTTCGTAAAGGGAACAGAACCTCATATAACGTGCTCTGACTTGAAT
GCCATACTGGGACTCAGATGA

Upstream 100 bases:

>100_bases
CCTCTGGGAGGAGTAGTAATCCTTTACCGCTTTTTCCATAAAGTCTCTGAAACTGATAAAAGCCATTATTTAGAATATAT
TAAATACTTTACGGGAGAAG

Downstream 100 bases:

>100_bases
GGTGGATTGAGGAGGAATTAAAGAGAATTAAGGAAGCAAACCTTTACAGGGAAAGGATACTCCTTGAGGGAGTAAAGGAT
TTCTGCTCCAATGATTACTT

Product: penicillin binding protein 1A

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase

Number of amino acids: Translated: 726; Mature: 726

Protein sequence:

>726_residues
MKKLVIGILGIVIALFVGLLVFLIPIYKNLPDPKLLESWTPPQASEVYDAKGRLYGTIGIQKRFYVSIDKIPEHVINAFV
ATEDRNFWHHFGIDPVAIVRAAIVNYRAGRIVQGGSTITQQLAKNLFLTRERTLERKIKEALLAIKIERTFDKKKIMELY
LNQIYLGSGAYGVEAAAQVYFGKHVWELSLDEAALLAALPKAPAKYNPFYHPERALQRRNLVLKRMLEEGYITPEQYEEA
VNKPLTVKKENKYKFSDYFLDMVKSYVFNKYGEIAYKGRLKIYTTIDLDYQKIAQKSLEEGLKRVAKIIGLPFLPKSEED
MELAYEKEAQLKRLKRGKIYVAKILKYDGNFMKVEIHGKKLKGEIKGLNTEGHKYVFVKYLGGNRAEIIPDLEGSLVSID
VKTGEIKAIVGGRSYAYSQFNRAVKALRQPGSAIKPVIYLSALLKGMTQISTIDASSKPYYDPSKGEDWIPKNYDEKEYG
NVTLRYALAHSINTAAVNLLDKVGFELVLEVGKKVGLDNLKPYYSLALGTVEVTPLQLTAAYQVFANLGTECKPFFIKKI
VDENGEVLEENVPECEEVLPKPETRVPVDMLRAVVLEGTARRASVLDRIVAGKTGTTDDFQDAWFVGFSPYIVTGVWVGY
DVKKSLGKHMSGSRVALPIWIDYMKVVTRMYPNEDFELPPENIVVNINPKDLVLADETCEGVPMVFVKGTEPHITCSDLN
AILGLR

Sequences:

>Translated_726_residues
MKKLVIGILGIVIALFVGLLVFLIPIYKNLPDPKLLESWTPPQASEVYDAKGRLYGTIGIQKRFYVSIDKIPEHVINAFV
ATEDRNFWHHFGIDPVAIVRAAIVNYRAGRIVQGGSTITQQLAKNLFLTRERTLERKIKEALLAIKIERTFDKKKIMELY
LNQIYLGSGAYGVEAAAQVYFGKHVWELSLDEAALLAALPKAPAKYNPFYHPERALQRRNLVLKRMLEEGYITPEQYEEA
VNKPLTVKKENKYKFSDYFLDMVKSYVFNKYGEIAYKGRLKIYTTIDLDYQKIAQKSLEEGLKRVAKIIGLPFLPKSEED
MELAYEKEAQLKRLKRGKIYVAKILKYDGNFMKVEIHGKKLKGEIKGLNTEGHKYVFVKYLGGNRAEIIPDLEGSLVSID
VKTGEIKAIVGGRSYAYSQFNRAVKALRQPGSAIKPVIYLSALLKGMTQISTIDASSKPYYDPSKGEDWIPKNYDEKEYG
NVTLRYALAHSINTAAVNLLDKVGFELVLEVGKKVGLDNLKPYYSLALGTVEVTPLQLTAAYQVFANLGTECKPFFIKKI
VDENGEVLEENVPECEEVLPKPETRVPVDMLRAVVLEGTARRASVLDRIVAGKTGTTDDFQDAWFVGFSPYIVTGVWVGY
DVKKSLGKHMSGSRVALPIWIDYMKVVTRMYPNEDFELPPENIVVNINPKDLVLADETCEGVPMVFVKGTEPHITCSDLN
AILGLR
>Mature_726_residues
MKKLVIGILGIVIALFVGLLVFLIPIYKNLPDPKLLESWTPPQASEVYDAKGRLYGTIGIQKRFYVSIDKIPEHVINAFV
ATEDRNFWHHFGIDPVAIVRAAIVNYRAGRIVQGGSTITQQLAKNLFLTRERTLERKIKEALLAIKIERTFDKKKIMELY
LNQIYLGSGAYGVEAAAQVYFGKHVWELSLDEAALLAALPKAPAKYNPFYHPERALQRRNLVLKRMLEEGYITPEQYEEA
VNKPLTVKKENKYKFSDYFLDMVKSYVFNKYGEIAYKGRLKIYTTIDLDYQKIAQKSLEEGLKRVAKIIGLPFLPKSEED
MELAYEKEAQLKRLKRGKIYVAKILKYDGNFMKVEIHGKKLKGEIKGLNTEGHKYVFVKYLGGNRAEIIPDLEGSLVSID
VKTGEIKAIVGGRSYAYSQFNRAVKALRQPGSAIKPVIYLSALLKGMTQISTIDASSKPYYDPSKGEDWIPKNYDEKEYG
NVTLRYALAHSINTAAVNLLDKVGFELVLEVGKKVGLDNLKPYYSLALGTVEVTPLQLTAAYQVFANLGTECKPFFIKKI
VDENGEVLEENVPECEEVLPKPETRVPVDMLRAVVLEGTARRASVLDRIVAGKTGTTDDFQDAWFVGFSPYIVTGVWVGY
DVKKSLGKHMSGSRVALPIWIDYMKVVTRMYPNEDFELPPENIVVNINPKDLVLADETCEGVPMVFVKGTEPHITCSDLN
AILGLR

Specific function: Cell Wall Formation. Synthesis Of Cross-Linked Peptidoglycan From The Lipid Intermediates. The Enzyme Has A Penicillin-Insensitive Transglycosylase N-Terminal Domain (Formation Of Linear Glycan Strands) And A Penicillin-Sensitive Transpeptidase C-Terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family

Homologues:

Organism=Escherichia coli, GI87082258, Length=833, Percent_Identity=32.6530612244898, Blast_Score=397, Evalue=1e-111,
Organism=Escherichia coli, GI1786343, Length=637, Percent_Identity=29.8273155416013, Blast_Score=242, Evalue=7e-65,
Organism=Escherichia coli, GI1788867, Length=301, Percent_Identity=29.5681063122924, Blast_Score=141, Evalue=2e-34,
Organism=Escherichia coli, GI1789601, Length=166, Percent_Identity=40.3614457831325, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PBPA_AQUAE (O66874)

Other databases:

- EMBL:   AE000657
- PIR:   F70355
- RefSeq:   NP_213434.1
- PDB:   2OQO
- PDB:   3D3H
- PDBsum:   2OQO
- PDBsum:   3D3H
- ProteinModelPortal:   O66874
- GeneID:   1193936
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_624
- NMPDR:   fig|224324.1.peg.449
- HOGENOM:   HBG672432
- OMA:   NISIARH
- PhylomeDB:   O66874
- ProtClustDB:   CLSK2299450
- BioCyc:   AAEO224324:AQ_624-MONOMER
- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460
- TIGRFAMs:   TIGR02074

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase; SSF56601 PBP_transp_fold

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 81825; Mature: 81825

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: ACT_SITE 432-432

Signals:

None

Transmembrane regions:

HASH(0xb06c2f0)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVIGILGIVIALFVGLLVFLIPIYKNLPDPKLLESWTPPQASEVYDAKGRLYGTIGI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHCCCCCEEEEEEC
QKRFYVSIDKIPEHVINAFVATEDRNFWHHFGIDPVAIVRAAIVNYRAGRIVQGGSTITQ
CEEEEEEHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCEEECCHHHHH
QLAKNLFLTRERTLERKIKEALLAIKIERTFDKKKIMELYLNQIYLGSGAYGVEAAAQVY
HHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHH
FGKHVWELSLDEAALLAALPKAPAKYNPFYHPERALQRRNLVLKRMLEEGYITPEQYEEA
HCCEEEEECCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VNKPLTVKKENKYKFSDYFLDMVKSYVFNKYGEIAYKGRLKIYTTIDLDYQKIAQKSLEE
HCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEECEEEEEEEECCCHHHHHHHHHHH
GLKRVAKIIGLPFLPKSEEDMELAYEKEAQLKRLKRGKIYVAKILKYDGNFMKVEIHGKK
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCEEEEEEECCE
LKGEIKGLNTEGHKYVFVKYLGGNRAEIIPDLEGSLVSIDVKTGEIKAIVGGRSYAYSQF
ECCEEECCCCCCCEEEEEEEECCCCCEECCCCCCCEEEEEEECCCEEEEECCCHHHHHHH
NRAVKALRQPGSAIKPVIYLSALLKGMTQISTIDASSKPYYDPSKGEDWIPKNYDEKEYG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCC
NVTLRYALAHSINTAAVNLLDKVGFELVLEVGKKVGLDNLKPYYSLALGTVEVTPLQLTA
CEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHEECEEEECCCHHHH
AYQVFANLGTECKPFFIKKIVDENGEVLEENVPECEEVLPKPETRVPVDMLRAVVLEGTA
HHHHHHHCCCCCCHHHHHHHHCCCCCCHHCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCH
RRASVLDRIVAGKTGTTDDFQDAWFVGFSPYIVTGVWVGYDVKKSLGKHMSGSRVALPIW
HHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHEEEEECCHHHHHHCCCCCCCEEEEHHH
IDYMKVVTRMYPNEDFELPPENIVVNINPKDLVLADETCEGVPMVFVKGTEPHITCSDLN
HHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCCEEEEECCCCCEEHHHHH
AILGLR
HHHCCH
>Mature Secondary Structure
MKKLVIGILGIVIALFVGLLVFLIPIYKNLPDPKLLESWTPPQASEVYDAKGRLYGTIGI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHCCCCCEEEEEEC
QKRFYVSIDKIPEHVINAFVATEDRNFWHHFGIDPVAIVRAAIVNYRAGRIVQGGSTITQ
CEEEEEEHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCEEECCHHHHH
QLAKNLFLTRERTLERKIKEALLAIKIERTFDKKKIMELYLNQIYLGSGAYGVEAAAQVY
HHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHH
FGKHVWELSLDEAALLAALPKAPAKYNPFYHPERALQRRNLVLKRMLEEGYITPEQYEEA
HCCEEEEECCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VNKPLTVKKENKYKFSDYFLDMVKSYVFNKYGEIAYKGRLKIYTTIDLDYQKIAQKSLEE
HCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEECEEEEEEEECCCHHHHHHHHHHH
GLKRVAKIIGLPFLPKSEEDMELAYEKEAQLKRLKRGKIYVAKILKYDGNFMKVEIHGKK
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCEEEEEEECCE
LKGEIKGLNTEGHKYVFVKYLGGNRAEIIPDLEGSLVSIDVKTGEIKAIVGGRSYAYSQF
ECCEEECCCCCCCEEEEEEEECCCCCEECCCCCCCEEEEEEECCCEEEEECCCHHHHHHH
NRAVKALRQPGSAIKPVIYLSALLKGMTQISTIDASSKPYYDPSKGEDWIPKNYDEKEYG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCC
NVTLRYALAHSINTAAVNLLDKVGFELVLEVGKKVGLDNLKPYYSLALGTVEVTPLQLTA
CEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHEECEEEECCCHHHH
AYQVFANLGTECKPFFIKKIVDENGEVLEENVPECEEVLPKPETRVPVDMLRAVVLEGTA
HHHHHHHCCCCCCHHHHHHHHCCCCCCHHCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCH
RRASVLDRIVAGKTGTTDDFQDAWFVGFSPYIVTGVWVGYDVKKSLGKHMSGSRVALPIW
HHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHEEEEECCHHHHHHCCCCCCCEEEEHHH
IDYMKVVTRMYPNEDFELPPENIVVNINPKDLVLADETCEGVPMVFVKGTEPHITCSDLN
HHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCCEEEEECCCCCEEHHHHH
AILGLR
HHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320