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The map label for this gene is rpoN [H]

Identifier: 15606041

GI number: 15606041

Start: 408354

End: 409550

Strand: Direct

Name: rpoN [H]

Synonym: aq_599

Alternate gene names: 15606041

Gene position: 408354-409550 (Clockwise)

Preceding gene: 15606037

Following gene: 15606043

Centisome position: 26.32

GC content: 37.01

Gene sequence:

>1197_bases
ATGTTAAATCAGAGATTAGAAGTAAGGCAAAAACTAAACTTAAAACTTTTACTGAAACAAGATCTTGAACTCCTCACTTA
TCAAACTCAGGAACTTGAAAAACTCATACATGAAGAGGTTCTCGTAAACCCTCTAATAAAAGGCGTTTTCAAGAAAATAC
CGAAAAGTTTTGAAGTAAAGGAAACGGTTCCCTACCAGATACCATACACACCCTCAGAACTGGAAGAACTCCAGCAGAAT
ATAAAACTGGAACTTGAAGGAAAAGAGCAGGAACTTGCTTTAGAGCTTTTGAATTATCTGAATGAAAAGGGCTTCCTATC
AAAAAGTGTTGAGGAGATATCCGACGTGCTACGGTGTTCTGTTGAAGAACTGGAAAAAGTTCGTCAAAAGGTGCTAAGAC
TTGAACCTTTGGGAGTTTGCTCAAAAGACGTGTGGGAGTTCTTAGAGTTACAGATTGAAGAAATTTACCCAGAAGAAGAG
GAAATTTTAAAAAAAGCCCTTCGGGATTTAAAAAGAGGAAAAAAGCTAAAACCAGAAATTAAGGGAAAACTCTCAAGGTT
AAGACTCTTTCCTCTTTCCAGTTCAGCGGAGAAAGTTTACACCTTTGCAAAAGTTGACGCAATTATAGAGGAAGAAAACG
GAGAGTTTTTTATATACCTTTATGAAGACTTTATAGATATAGACTTAAACGAAGAGTACTGGGAACTTTACAAAAAGTCA
AGAAATCTCCAAAAGGAACTAAAAGAGGCCTTTGAGAGGTACGAAAGTATAAGGAAGGTTCTGGATATAAGAAGAAGGAA
TTTAAGGAAAGTTTTAGAGAAAATCGTTGAAAGGCAAAAGGATTTTTTAACGGGAAAGGGAAGTTTAAAACCGCTGACTT
TGCGTGAAGTTTCAAGTGAGATAGGAATACACGAATCTACATTGAGCAGGATAGTGAACTCAAAGTACGTGAAAACTCCC
GTGGGAACTTACTCCTTGAGAACTTTCTTTGTGAGAGAGTCGGCGGAGGGATTAACGCAAGGTGAGTTAATGAAATTAAT
CAAGGAAATTGTAGAAAACGAAGATAAAAGGAAACCTTACAGTGACCAAGAAATAGCCAATATTCTAAAAGAGAAAGGCT
TTAAAGTTGCGAGGAGAACGGTTGCAAAATACAGGGAAATGTTAGGAATTCCCTCCTCAAGGGAAAGAAGGATTTAA

Upstream 100 bases:

>100_bases
CCTCCTTATAGATTTTTTAAAAATAGTATTCACTAACCATTCTATCATTTCTATGACTTTAAACATTAACCGAAAGGGAT
AAAATTTTTAATCGTGGGAG

Downstream 100 bases:

>100_bases
AAGGGAGAAAGGGAGATTAAGCCACTTCTCCTTGTTCTTCTAGTTTCTTTATGGCTATGCTGAGTTGGTGAAGAAGGTCT
GCAGGTGATTCTACTTGTTC

Product: RNA polymerase sigma factor RpoN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MLNQRLEVRQKLNLKLLLKQDLELLTYQTQELEKLIHEEVLVNPLIKGVFKKIPKSFEVKETVPYQIPYTPSELEELQQN
IKLELEGKEQELALELLNYLNEKGFLSKSVEEISDVLRCSVEELEKVRQKVLRLEPLGVCSKDVWEFLELQIEEIYPEEE
EILKKALRDLKRGKKLKPEIKGKLSRLRLFPLSSSAEKVYTFAKVDAIIEEENGEFFIYLYEDFIDIDLNEEYWELYKKS
RNLQKELKEAFERYESIRKVLDIRRRNLRKVLEKIVERQKDFLTGKGSLKPLTLREVSSEIGIHESTLSRIVNSKYVKTP
VGTYSLRTFFVRESAEGLTQGELMKLIKEIVENEDKRKPYSDQEIANILKEKGFKVARRTVAKYREMLGIPSSRERRI

Sequences:

>Translated_398_residues
MLNQRLEVRQKLNLKLLLKQDLELLTYQTQELEKLIHEEVLVNPLIKGVFKKIPKSFEVKETVPYQIPYTPSELEELQQN
IKLELEGKEQELALELLNYLNEKGFLSKSVEEISDVLRCSVEELEKVRQKVLRLEPLGVCSKDVWEFLELQIEEIYPEEE
EILKKALRDLKRGKKLKPEIKGKLSRLRLFPLSSSAEKVYTFAKVDAIIEEENGEFFIYLYEDFIDIDLNEEYWELYKKS
RNLQKELKEAFERYESIRKVLDIRRRNLRKVLEKIVERQKDFLTGKGSLKPLTLREVSSEIGIHESTLSRIVNSKYVKTP
VGTYSLRTFFVRESAEGLTQGELMKLIKEIVENEDKRKPYSDQEIANILKEKGFKVARRTVAKYREMLGIPSSRERRI
>Mature_398_residues
MLNQRLEVRQKLNLKLLLKQDLELLTYQTQELEKLIHEEVLVNPLIKGVFKKIPKSFEVKETVPYQIPYTPSELEELQQN
IKLELEGKEQELALELLNYLNEKGFLSKSVEEISDVLRCSVEELEKVRQKVLRLEPLGVCSKDVWEFLELQIEEIYPEEE
EILKKALRDLKRGKKLKPEIKGKLSRLRLFPLSSSAEKVYTFAKVDAIIEEENGEFFIYLYEDFIDIDLNEEYWELYKKS
RNLQKELKEAFERYESIRKVLDIRRRNLRKVLEKIVERQKDFLTGKGSLKPLTLREVSSEIGIHESTLSRIVNSKYVKTP
VGTYSLRTFFVRESAEGLTQGELMKLIKEIVENEDKRKPYSDQEIANILKEKGFKVARRTVAKYREMLGIPSSRERRI

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the levanase operon. The open complex (sigma-54 and core RNA poly

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=338, Percent_Identity=27.5147928994083, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 47005; Mature: 47005

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNQRLEVRQKLNLKLLLKQDLELLTYQTQELEKLIHEEVLVNPLIKGVFKKIPKSFEVK
CCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ETVPYQIPYTPSELEELQQNIKLELEGKEQELALELLNYLNEKGFLSKSVEEISDVLRCS
CCCCCCCCCCHHHHHHHHHCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
VEELEKVRQKVLRLEPLGVCSKDVWEFLELQIEEIYPEEEEILKKALRDLKRGKKLKPEI
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHH
KGKLSRLRLFPLSSSAEKVYTFAKVDAIIEEENGEFFIYLYEDFIDIDLNEEYWELYKKS
HHHHHHHEEECCCCCHHHHHHHHHHHHHHEECCCCEEEEEECCHHCCCCCHHHHHHHHHH
RNLQKELKEAFERYESIRKVLDIRRRNLRKVLEKIVERQKDFLTGKGSLKPLTLREVSSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
IGIHESTLSRIVNSKYVKTPVGTYSLRTFFVRESAEGLTQGELMKLIKEIVENEDKRKPY
HCCCHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCC
SDQEIANILKEKGFKVARRTVAKYREMLGIPSSRERRI
CHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLNQRLEVRQKLNLKLLLKQDLELLTYQTQELEKLIHEEVLVNPLIKGVFKKIPKSFEVK
CCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ETVPYQIPYTPSELEELQQNIKLELEGKEQELALELLNYLNEKGFLSKSVEEISDVLRCS
CCCCCCCCCCHHHHHHHHHCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
VEELEKVRQKVLRLEPLGVCSKDVWEFLELQIEEIYPEEEEILKKALRDLKRGKKLKPEI
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHH
KGKLSRLRLFPLSSSAEKVYTFAKVDAIIEEENGEFFIYLYEDFIDIDLNEEYWELYKKS
HHHHHHHEEECCCCCHHHHHHHHHHHHHHEECCCCEEEEEECCHHCCCCCHHHHHHHHHH
RNLQKELKEAFERYESIRKVLDIRRRNLRKVLEKIVERQKDFLTGKGSLKPLTLREVSSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
IGIHESTLSRIVNSKYVKTPVGTYSLRTFFVRESAEGLTQGELMKLIKEIVENEDKRKPY
HCCCHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCC
SDQEIANILKEKGFKVARRTVAKYREMLGIPSSRERRI
CHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1924373; 8969506; 9384377 [H]