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The map label for this gene is dosC [H]

Identifier: 15606018

GI number: 15606018

Start: 383468

End: 385324

Strand: Direct

Name: dosC [H]

Synonym: aq_563

Alternate gene names: 15606018

Gene position: 383468-385324 (Clockwise)

Preceding gene: 15606014

Following gene: 15606019

Centisome position: 24.72

GC content: 43.4

Gene sequence:

>1857_bases
GTGAGACACAATTTATTGTTAAAACTCCTCGCTCTTTGGGGAGTATCCCTTACGATCCTACTCGTATTCGCCCTTATCTT
TATAAGAAAGTCCACCTTGAAACCTGCAAAAGACAGGGCTTTAACCACGGCACAACTCGTGAGGGATGCCCTCACCACTC
TTATGGTTTCAAACGCTATGGACAAAAGGGAAATTTTCTTAGAGGAAATTAAAAAGGTTCAAGGACTCAAAGAAATTCGC
GTAATAAGGGGGGAGGCTGTAGTAAAGCAGTACGGTCCGGGACTTTACTCGGAAAGACCAAAAGATCCTCTGGAAAAGGA
AGTTCTCGCCACGGGCAAGGTAAAGGAGGAAATTCTGGAGGATATTAACAGAGTTTTGTACAGGGTTATAATACCTTACA
AGGCGGAGCCTTCACCCATTTTAAACTGTATGCAGTGCCACAAAGTAAAGCCCGGAGAGGTTCTCGGAGCTGTCTCCATT
ACGATGGATCTAACTCCTTACAGGAATTTCGGCTTTTTCATTTTCTTCAGTCTCGGTGCTATAGCCCTCACGGTGGCTTT
CATTACTTTCCTTATCGTTTACGGAAACATCAAGAACGTCAGCACATTTATAGACGAACTGGACAAAGTCTTTTCGGCTT
TTGAAAAGGGGATATTCTACGAAAGGCTCAGTTTGGAAACCTTAAAAGGTGAGATAAAAGATTTCGGAAAGAAGATTAAT
AAAGTACTGGACATACTTGACAACGCCTTTAAGGATATAAGGGAAAAGGTTTACGCACTCCTTGGATACTCAATCATGGA
AACGGAAAACGAACTGAGGGACACCGAGAAAGTAATAGACGAGCTCGTGAGGATTTCTCAGTTCAAGAGAACGATAGAAC
TGGACAACTCCCTTGAAGAGGTTTACAAGAGGCTTGAGAGCGTTCTTTCTAACTACATGAGTCTAGATAAGTTCTCTATA
TACGAAGTGACCGACGACAACAGGATTAAAAAGGTTGCCGTACACGGAAGAGAACTCTGGTGTTCGGAAATCATACTGAA
AGACGCAAACGAGTGCAGGGCAAAGAGAACGGGGCAGGACGTGGACTCAGAAGAGTTCGAGTGTGTATGTCCAAACTTTG
CGTACTGCGCTAACGAAAACTCCAATAAAAAGTACTGTTACTACTGCATACCTGTTAACGTAGGAGGAAAAATAGGAAAC
GTGGTTCAATTGGTTTATGAAAGGGAATTGAAGGACTTCGTGAAGCTCCTCATTCCTTACATAAAAGGGTACTTAAGGGA
AGCGACTCCCGTTATAGAGTCCAAGAAATTAATGGAAAAGCTCAAGATGCAGTCTTACATAGACGGGCTTACCGGGGCTT
TTAACAGGCGCTTTCTGGAAGAAATAGCGGACAAAATAGTGGCTCAAACTCTGAGGAGAAACTCAAACCTCGGCATACTT
ATGATAGACGTTGATTTCTTTAAGCAGGTGAACGACACATACGGACACGACGCGGGTGATGAGGTCCTGAGACAAATAGC
AAGGGTAATAAAGGATAACATCAGGAAGGCGGATTACTTGATAAGGTACGGAGGAGAAGAATTTTTGGTTCTCCTTACAG
ATGTAAAGGAAGGTTACGCTGAGAAGGTAGCAGAGAAGCTCAGGAAAATAATTGAAGAAACGCCGATAACACTTCCCAAC
TGTCAGACTATCAGGAAAACAGTAAGCATAGGGATATCCGAATTTCCAAAAGATTGCGAAGGAAAGTTCTGGAAGTGTGT
TAAATTTGCAGACGTTGCCCTCTACAAGGCTAAGGAAGAGGGGAGGAACAGGGTGGTCCGCTTCAAGCCCGAAATGTGGC
AGGAGAAGGAGTATTAA

Upstream 100 bases:

>100_bases
ACCGCTGAGCTACGCCGGCTGAGTATATTAATTTAAACATTCATAATTTCCTTTTCAACACCTGAAAAATTCGGGAAATA
ATTTAAAGTATTAAATTATT

Downstream 100 bases:

>100_bases
AATAATCCCGATGCTATCCGAAGAAAGGGTAAAGGAACTTCTTAAAAAACTCAAAGATTTAAAAATCCTCGTGGTGGGAG
ACGTAATCCTTGACACCTAC

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 618; Mature: 618

Protein sequence:

>618_residues
MRHNLLLKLLALWGVSLTILLVFALIFIRKSTLKPAKDRALTTAQLVRDALTTLMVSNAMDKREIFLEEIKKVQGLKEIR
VIRGEAVVKQYGPGLYSERPKDPLEKEVLATGKVKEEILEDINRVLYRVIIPYKAEPSPILNCMQCHKVKPGEVLGAVSI
TMDLTPYRNFGFFIFFSLGAIALTVAFITFLIVYGNIKNVSTFIDELDKVFSAFEKGIFYERLSLETLKGEIKDFGKKIN
KVLDILDNAFKDIREKVYALLGYSIMETENELRDTEKVIDELVRISQFKRTIELDNSLEEVYKRLESVLSNYMSLDKFSI
YEVTDDNRIKKVAVHGRELWCSEIILKDANECRAKRTGQDVDSEEFECVCPNFAYCANENSNKKYCYYCIPVNVGGKIGN
VVQLVYERELKDFVKLLIPYIKGYLREATPVIESKKLMEKLKMQSYIDGLTGAFNRRFLEEIADKIVAQTLRRNSNLGIL
MIDVDFFKQVNDTYGHDAGDEVLRQIARVIKDNIRKADYLIRYGGEEFLVLLTDVKEGYAEKVAEKLRKIIEETPITLPN
CQTIRKTVSIGISEFPKDCEGKFWKCVKFADVALYKAKEEGRNRVVRFKPEMWQEKEY

Sequences:

>Translated_618_residues
MRHNLLLKLLALWGVSLTILLVFALIFIRKSTLKPAKDRALTTAQLVRDALTTLMVSNAMDKREIFLEEIKKVQGLKEIR
VIRGEAVVKQYGPGLYSERPKDPLEKEVLATGKVKEEILEDINRVLYRVIIPYKAEPSPILNCMQCHKVKPGEVLGAVSI
TMDLTPYRNFGFFIFFSLGAIALTVAFITFLIVYGNIKNVSTFIDELDKVFSAFEKGIFYERLSLETLKGEIKDFGKKIN
KVLDILDNAFKDIREKVYALLGYSIMETENELRDTEKVIDELVRISQFKRTIELDNSLEEVYKRLESVLSNYMSLDKFSI
YEVTDDNRIKKVAVHGRELWCSEIILKDANECRAKRTGQDVDSEEFECVCPNFAYCANENSNKKYCYYCIPVNVGGKIGN
VVQLVYERELKDFVKLLIPYIKGYLREATPVIESKKLMEKLKMQSYIDGLTGAFNRRFLEEIADKIVAQTLRRNSNLGIL
MIDVDFFKQVNDTYGHDAGDEVLRQIARVIKDNIRKADYLIRYGGEEFLVLLTDVKEGYAEKVAEKLRKIIEETPITLPN
CQTIRKTVSIGISEFPKDCEGKFWKCVKFADVALYKAKEEGRNRVVRFKPEMWQEKEY
>Mature_618_residues
MRHNLLLKLLALWGVSLTILLVFALIFIRKSTLKPAKDRALTTAQLVRDALTTLMVSNAMDKREIFLEEIKKVQGLKEIR
VIRGEAVVKQYGPGLYSERPKDPLEKEVLATGKVKEEILEDINRVLYRVIIPYKAEPSPILNCMQCHKVKPGEVLGAVSI
TMDLTPYRNFGFFIFFSLGAIALTVAFITFLIVYGNIKNVSTFIDELDKVFSAFEKGIFYERLSLETLKGEIKDFGKKIN
KVLDILDNAFKDIREKVYALLGYSIMETENELRDTEKVIDELVRISQFKRTIELDNSLEEVYKRLESVLSNYMSLDKFSI
YEVTDDNRIKKVAVHGRELWCSEIILKDANECRAKRTGQDVDSEEFECVCPNFAYCANENSNKKYCYYCIPVNVGGKIGN
VVQLVYERELKDFVKLLIPYIKGYLREATPVIESKKLMEKLKMQSYIDGLTGAFNRRFLEEIADKIVAQTLRRNSNLGIL
MIDVDFFKQVNDTYGHDAGDEVLRQIARVIKDNIRKADYLIRYGGEEFLVLLTDVKEGYAEKVAEKLRKIIEETPITLPN
CQTIRKTVSIGISEFPKDCEGKFWKCVKFADVALYKAKEEGRNRVVRFKPEMWQEKEY

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=168, Percent_Identity=40.4761904761905, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1786584, Length=190, Percent_Identity=34.2105263157895, Blast_Score=116, Evalue=5e-27,
Organism=Escherichia coli, GI87081881, Length=177, Percent_Identity=38.4180790960452, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI87082007, Length=169, Percent_Identity=35.5029585798817, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1787541, Length=190, Percent_Identity=31.0526315789474, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1787262, Length=161, Percent_Identity=38.5093167701863, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1787802, Length=172, Percent_Identity=34.3023255813954, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788381, Length=184, Percent_Identity=32.0652173913043, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1787816, Length=170, Percent_Identity=35.2941176470588, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI87081974, Length=118, Percent_Identity=33.0508474576271, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1788085, Length=181, Percent_Identity=29.2817679558011, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788956, Length=163, Percent_Identity=31.2883435582822, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081977, Length=188, Percent_Identity=27.1276595744681, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1787056, Length=174, Percent_Identity=29.8850574712644, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 71237; Mature: 71237

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHNLLLKLLALWGVSLTILLVFALIFIRKSTLKPAKDRALTTAQLVRDALTTLMVSNAM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
DKREIFLEEIKKVQGLKEIRVIRGEAVVKQYGPGLYSERPKDPLEKEVLATGKVKEEILE
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHH
DINRVLYRVIIPYKAEPSPILNCMQCHKVKPGEVLGAVSITMDLTPYRNFGFFIFFSLGA
HHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHEEEEEEEECCCCCCCHHHHHHHHHH
IALTVAFITFLIVYGNIKNVSTFIDELDKVFSAFEKGIFYERLSLETLKGEIKDFGKKIN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
KVLDILDNAFKDIREKVYALLGYSIMETENELRDTEKVIDELVRISQFKRTIELDNSLEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
VYKRLESVLSNYMSLDKFSIYEVTDDNRIKKVAVHGRELWCSEIILKDANECRAKRTGQD
HHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHCCHHHHHHHHHHHCHHHHHHHHCCCC
VDSEEFECVCPNFAYCANENSNKKYCYYCIPVNVGGKIGNVVQLVYERELKDFVKLLIPY
CCCCCCEEECCCCHHHCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
IKGYLREATPVIESKKLMEKLKMQSYIDGLTGAFNRRFLEEIADKIVAQTLRRNSNLGIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
MIDVDFFKQVNDTYGHDAGDEVLRQIARVIKDNIRKADYLIRYGGEEFLVLLTDVKEGYA
EEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHH
EKVAEKLRKIIEETPITLPNCQTIRKTVSIGISEFPKDCEGKFWKCVKFADVALYKAKEE
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
GRNRVVRFKPEMWQEKEY
CCCCEEEECCHHCCCCCC
>Mature Secondary Structure
MRHNLLLKLLALWGVSLTILLVFALIFIRKSTLKPAKDRALTTAQLVRDALTTLMVSNAM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
DKREIFLEEIKKVQGLKEIRVIRGEAVVKQYGPGLYSERPKDPLEKEVLATGKVKEEILE
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHH
DINRVLYRVIIPYKAEPSPILNCMQCHKVKPGEVLGAVSITMDLTPYRNFGFFIFFSLGA
HHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHEEEEEEEECCCCCCCHHHHHHHHHH
IALTVAFITFLIVYGNIKNVSTFIDELDKVFSAFEKGIFYERLSLETLKGEIKDFGKKIN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
KVLDILDNAFKDIREKVYALLGYSIMETENELRDTEKVIDELVRISQFKRTIELDNSLEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
VYKRLESVLSNYMSLDKFSIYEVTDDNRIKKVAVHGRELWCSEIILKDANECRAKRTGQD
HHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHCCHHHHHHHHHHHCHHHHHHHHCCCC
VDSEEFECVCPNFAYCANENSNKKYCYYCIPVNVGGKIGNVVQLVYERELKDFVKLLIPY
CCCCCCEEECCCCHHHCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
IKGYLREATPVIESKKLMEKLKMQSYIDGLTGAFNRRFLEEIADKIVAQTLRRNSNLGIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
MIDVDFFKQVNDTYGHDAGDEVLRQIARVIKDNIRKADYLIRYGGEEFLVLLTDVKEGYA
EEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHH
EKVAEKLRKIIEETPITLPNCQTIRKTVSIGISEFPKDCEGKFWKCVKFADVALYKAKEE
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
GRNRVVRFKPEMWQEKEY
CCCCEEEECCHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]