Click here to switch to the map view.

The map label for this gene is nasA [H]

Identifier: 15605772

GI number: 15605772

Start: 131158

End: 132585

Strand: Direct

Name: nasA [H]

Synonym: aq_215

Alternate gene names: 15605772

Gene position: 131158-132585 (Clockwise)

Preceding gene: 15605771

Following gene: 15605773

Centisome position: 8.45

GC content: 46.15

Gene sequence:

>1428_bases
ATGAATTTCCTGAAGGAGATAATTGAAGCAATTAAGAAGGGAAATCCAAAAGCTCTGTTTGCCAGTTTTATATACTTTGA
CCATTCATTCAGTATATGGCTACTCTTAGGAGCTCTTGGACCTTTTATAGCTGAGTCCTTCGGACTCTCAGGAGCCCAAA
AAGGTTTTATGGTTGCAATTCCCGTAATAGCCGCAGCCATATTCAGACTCAACTTCGGACATATGTTCCAGTATATAGAC
GGGAAGTACATAGCGATAATGGGCATAATCCTTTCCTCTATCCCTCACCTTTACATGATACTCAGCGGATATCGTGTAAC
CTACGATGACCTTCTCTGGATGGGGGTTTTTCTCGGTATCGCAGGAGCAAGCTTTGCGATAGCCCTCCCTATGGCGGGAA
GTAACTACCCGAAAGAGGTTCAAGGACTCGTACTTGGACTTGCCGCGGCGGGAAACATAGGAGCTGTTTTAGACGGTATA
CTCTTTCCTCCTATTGCAAGGTCCATCGGTTGGGAAAATACCTTCGTTCTTTCGGGAATACTCCTTCTTATAGCCCTGTT
TTTTGTCGTTATCTGGGCGCGGGACCTTACCAAGAAGAACGAAGGGAATAGGTTTTATCCCGTATTTGCATTCTTTGCCA
CTATAGCTTTTATGGTAATACTGGCTCTCGGTTTTCAAAAGGGATGGTTCGGTATAACCGGAAACGCTGGAAAACTCCTC
ATACCGGTACTTGGTTCTGTATTTGCTATACTCCTTATGCCTCTTGCCTTTAAAAAGGTGTTCCTGGAAAGGGACACGTG
GGTACTTATGCTCGCTTACTCCATAACTTTCGGCGGTTTTGTAGGAATGTCCTCGTACGTGGCTATGCTCCTTAGGGACA
TATACGGTATATCCAAAGTGGAGGCAGGAGCTCTTATGTCCCTTTTTGCCTTTACGGGTGCGATGATAAGACCTCTGGGG
GGACATATCGCGGATAAAATAAGCGGAGCTACAGCACTTCTCTTTTTCCTCGCGGGCATAGCGGGAGTTAACTTCATATT
TTCCCTCGTAACACCGCCTCTGGCGGTAGGTATAGTTTTATTCCTTGCACTCTACTCCTTCTATGGACTTGGAAACGGTG
CTGTATTTCAGCTCGTTCCTCACAGGTGGCCATATCAAACTGGACTTATGACGGGAATAATCGGAGCTGCCGGAGGTATC
GGTGGTTTTTACCTGCCCGTTGTAAACGGAATAGTCTTTGAGTCCACGGGAGCTTACAACCTGGCGTTTGCACTCTTCGG
AGGAATAGCCCTGCTGTGTCTCCTTATAGTTAAGCTTCTTCATGCTCGCTGGATGGAATGGGCCTACGTTCGCTACGACT
ACGAAAAAGAAACGTTGGTTGGTATTGATCCTAAGAGTGGAAGGGTAATTATGGAGTTGGCAAGATGA

Upstream 100 bases:

>100_bases
GCATAACGAAAGCTGCGCACACGGGTGCTTACGGAGATGGGAAGGTGTTCGTTTGCAGCATTTCAAAAGTTTGGACCATA
AGGACTAAGGAGGAGGAGCT

Downstream 100 bases:

>100_bases
AGAGGTTTCAATGTCCTTACTGCGGTGTAGGATGCGGTCTTTACATAAACGAGAAGGGTAAGGTGAAAGGGGATTTTGAA
CATCCCGCTAATCTCGGGGA

Product: nitrate transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MNFLKEIIEAIKKGNPKALFASFIYFDHSFSIWLLLGALGPFIAESFGLSGAQKGFMVAIPVIAAAIFRLNFGHMFQYID
GKYIAIMGIILSSIPHLYMILSGYRVTYDDLLWMGVFLGIAGASFAIALPMAGSNYPKEVQGLVLGLAAAGNIGAVLDGI
LFPPIARSIGWENTFVLSGILLLIALFFVVIWARDLTKKNEGNRFYPVFAFFATIAFMVILALGFQKGWFGITGNAGKLL
IPVLGSVFAILLMPLAFKKVFLERDTWVLMLAYSITFGGFVGMSSYVAMLLRDIYGISKVEAGALMSLFAFTGAMIRPLG
GHIADKISGATALLFFLAGIAGVNFIFSLVTPPLAVGIVLFLALYSFYGLGNGAVFQLVPHRWPYQTGLMTGIIGAAGGI
GGFYLPVVNGIVFESTGAYNLAFALFGGIALLCLLIVKLLHARWMEWAYVRYDYEKETLVGIDPKSGRVIMELAR

Sequences:

>Translated_475_residues
MNFLKEIIEAIKKGNPKALFASFIYFDHSFSIWLLLGALGPFIAESFGLSGAQKGFMVAIPVIAAAIFRLNFGHMFQYID
GKYIAIMGIILSSIPHLYMILSGYRVTYDDLLWMGVFLGIAGASFAIALPMAGSNYPKEVQGLVLGLAAAGNIGAVLDGI
LFPPIARSIGWENTFVLSGILLLIALFFVVIWARDLTKKNEGNRFYPVFAFFATIAFMVILALGFQKGWFGITGNAGKLL
IPVLGSVFAILLMPLAFKKVFLERDTWVLMLAYSITFGGFVGMSSYVAMLLRDIYGISKVEAGALMSLFAFTGAMIRPLG
GHIADKISGATALLFFLAGIAGVNFIFSLVTPPLAVGIVLFLALYSFYGLGNGAVFQLVPHRWPYQTGLMTGIIGAAGGI
GGFYLPVVNGIVFESTGAYNLAFALFGGIALLCLLIVKLLHARWMEWAYVRYDYEKETLVGIDPKSGRVIMELAR
>Mature_475_residues
MNFLKEIIEAIKKGNPKALFASFIYFDHSFSIWLLLGALGPFIAESFGLSGAQKGFMVAIPVIAAAIFRLNFGHMFQYID
GKYIAIMGIILSSIPHLYMILSGYRVTYDDLLWMGVFLGIAGASFAIALPMAGSNYPKEVQGLVLGLAAAGNIGAVLDGI
LFPPIARSIGWENTFVLSGILLLIALFFVVIWARDLTKKNEGNRFYPVFAFFATIAFMVILALGFQKGWFGITGNAGKLL
IPVLGSVFAILLMPLAFKKVFLERDTWVLMLAYSITFGGFVGMSSYVAMLLRDIYGISKVEAGALMSLFAFTGAMIRPLG
GHIADKISGATALLFFLAGIAGVNFIFSLVTPPLAVGIVLFLALYSFYGLGNGAVFQLVPHRWPYQTGLMTGIIGAAGGI
GGFYLPVVNGIVFESTGAYNLAFALFGGIALLCLLIVKLLHARWMEWAYVRYDYEKETLVGIDPKSGRVIMELAR

Specific function: May function as a nitrate transporter [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=451, Percent_Identity=23.059866962306, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51528; Mature: 51528

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFLKEIIEAIKKGNPKALFASFIYFDHSFSIWLLLGALGPFIAESFGLSGAQKGFMVAI
CCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHH
PVIAAAIFRLNFGHMFQYIDGKYIAIMGIILSSIPHLYMILSGYRVTYDDLLWMGVFLGI
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AGASFAIALPMAGSNYPKEVQGLVLGLAAAGNIGAVLDGILFPPIARSIGWENTFVLSGI
CCCHHEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LLLIALFFVVIWARDLTKKNEGNRFYPVFAFFATIAFMVILALGFQKGWFGITGNAGKLL
HHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHH
IPVLGSVFAILLMPLAFKKVFLERDTWVLMLAYSITFGGFVGMSSYVAMLLRDIYGISKV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
EAGALMSLFAFTGAMIRPLGGHIADKISGATALLFFLAGIAGVNFIFSLVTPPLAVGIVL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
FLALYSFYGLGNGAVFQLVPHRWPYQTGLMTGIIGAAGGIGGFYLPVVNGIVFESTGAYN
HHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCEEEECCCCHH
LAFALFGGIALLCLLIVKLLHARWMEWAYVRYDYEKETLVGIDPKSGRVIMELAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEECCCCCCCEEEEECC
>Mature Secondary Structure
MNFLKEIIEAIKKGNPKALFASFIYFDHSFSIWLLLGALGPFIAESFGLSGAQKGFMVAI
CCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHH
PVIAAAIFRLNFGHMFQYIDGKYIAIMGIILSSIPHLYMILSGYRVTYDDLLWMGVFLGI
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AGASFAIALPMAGSNYPKEVQGLVLGLAAAGNIGAVLDGILFPPIARSIGWENTFVLSGI
CCCHHEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LLLIALFFVVIWARDLTKKNEGNRFYPVFAFFATIAFMVILALGFQKGWFGITGNAGKLL
HHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHH
IPVLGSVFAILLMPLAFKKVFLERDTWVLMLAYSITFGGFVGMSSYVAMLLRDIYGISKV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
EAGALMSLFAFTGAMIRPLGGHIADKISGATALLFFLAGIAGVNFIFSLVTPPLAVGIVL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
FLALYSFYGLGNGAVFQLVPHRWPYQTGLMTGIIGAAGGIGGFYLPVVNGIVFESTGAYN
HHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCEEEECCCCHH
LAFALFGGIALLCLLIVKLLHARWMEWAYVRYDYEKETLVGIDPKSGRVIMELAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEECCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]