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The map label for this gene is narB [H]

Identifier: 15605773

GI number: 15605773

Start: 132582

End: 134582

Strand: Direct

Name: narB [H]

Synonym: aq_217

Alternate gene names: 15605773

Gene position: 132582-134582 (Clockwise)

Preceding gene: 15605772

Following gene: 15605779

Centisome position: 8.55

GC content: 44.53

Gene sequence:

>2001_bases
ATGAAGAGGTTTCAATGTCCTTACTGCGGTGTAGGATGCGGTCTTTACATAAACGAGAAGGGTAAGGTGAAAGGGGATTT
TGAACATCCCGCTAATCTCGGGGACATCTGTAAGAAGCCCTTGTATTTACCGAAGGTACTGAATAGGGGAAGGATAGCGA
AGCCCATGTACAGGGAAAATAAGAGTCACAGCTTCAGGGAAATAAGTTGGGAGGAAGCTTACGAGATACTAAAAGAGAAG
CTCATCTCCTTTTCTCCCGACGAAACTTACTTTTATCTTTCGGGACAACTTTTAACGGAGGATATATACGTAGCAAACAA
GTTCGTTAAAGGTTTTCTCAGAACCAATAACGTTGACGCAAACTCAAGACTTTGTATGGCTTCTGCAGTTACCGCTTACA
AACTCGCTTTTGGTTCCGACGGTGTTCCCTGTACGTACGAGGACGTGGACGATGCGGATGCCTTCCTTTTTATAGGTTCT
AACGCTGCCGTAGCTCATCCTGTTCTTTTTAAAAGGGTTCTGAAGAGGAGGAAGGAAGAAGAAAAAGTAATTATAGTAAC
CGTAGATCCGCTGGAAACGGAAACCGCCAAAAGGAGCGATGTTTTCATTCAAATAAAAGCTGGAACGGATACGGTTTTCC
TCAACTCCGTTTTATACGTTCTGCATAAGGAGGGGTGGATAGATTACCGCTTTATTTACAACTACACGGAGGGATTTGAA
GAGGCTCTGGAAGTTGCCATGAAATTCCCTCCAGATGTGGCCGCCAGCATTTGTAGCGTCAAACCGGAAGACGTATACCT
CGTAGCGGAGCTCTTTGCCCACAGTAAAAAACTTATCTCCTTCTGGTGTCAAGGGTTAAATCAGTCCTTAAATGGGACTA
TGAAAAATCTGGCTTTGATAAACCTCCACTTGGCCACCGGAAGGCTCAACGAAAAGGGATGTCCCTTTTCCTTAACCGGT
CAACCGAACGCCATGGGAGGTAGAGAAGTAGGTTATCTCACAAACGGTCTTCCCGGATACAGAGATGTCAGGAATAAGGC
GGACAGAGCATTTATGGAGAAATTCTGGGGCGTTAAAGGTATAAAGAAAGAGCCGGGACCCACAATTACGGAAGCGATAG
ACATGATTCTAAAAGACAGAATAAAGTTTCTCTGGGTGGTATGCACAAATCCCGCGGTGTCTTTACCCAACTTAGAAAAA
GTTAAAAGGGCTCTTGAGAAGGTTTTCTTAGTAGTTCAGGACGCTTACTGGAATGACACCTGCGAGTTTGCAAATTTAAT
ACTGCCCGCCGCACAACTGGGAGAAAAAGAAGGAGTTATGACTGGTTCCGATAGAACTGTTACATTTTGTGAGCGTTTTT
CCGAGCCTTTTGGTGAATCAAAGCCAGACTGGCTCATATTTACTGAGCTTGCAAGAAAGATGGGAGCCGAAGAGCTTTTC
CCTTACTCTTCTCCAAAGGAAATATTTGAGGAATTTAAAGCCTCAACCAAGGGGAGATTGTGCGACATGAGTAATTTATC
CTACGAAAACCTTCCCGCAAGGTGGGGCAAAAGATGGCTCTATGAAGACCTAAAATTCCCAACGGAAAGCGGAAAAGCAA
GATTTCACCCCGCACGGGTAGATGTGGCAGCTGAAGAAGGGATGTTTATACTTCTGACGGGAAGATTAAAGAACAACTGG
CACACTATGACGAGAACTGGAAAGAGCCCGGAACTGTTGAAAGGTGAAATTCCACCTTTTGTGATAATGAATCCCAGAGA
CGCGGAGGAACTGGGAATAGAGGAAGGAGAGGAAGTGATACTGAGTGCAAAGGGAAAAGAAATAACCAGAGTTGTCAGGT
TCGGAAACGTAAAAAGAGGACACATATTCGCACCTTTCGGTTATCCTTCCGAATACGGAGAGCCTACTAATTTGTTAACG
TCCGACAGAACGGATCCTTACTCAAAGGAGCCGGATCTTAAGTACTCAGGGGTAGATGTGTTTGTAAAAGTAAATAGGTG
A

Upstream 100 bases:

>100_bases
CTCGCTGGATGGAATGGGCCTACGTTCGCTACGACTACGAAAAAGAAACGTTGGTTGGTATTGATCCTAAGAGTGGAAGG
GTAATTATGGAGTTGGCAAG

Downstream 100 bases:

>100_bases
ATTTACCTTTTTTTGGGGAGTTTTATTCCGTACTTTTGTATCCTGTAACTCACCTGTCTCGGCGTCATACCTAGTTTTTT
GGCGGCTTCTTTTATGACAA

Product: nitrate reductase narB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 666; Mature: 666

Protein sequence:

>666_residues
MKRFQCPYCGVGCGLYINEKGKVKGDFEHPANLGDICKKPLYLPKVLNRGRIAKPMYRENKSHSFREISWEEAYEILKEK
LISFSPDETYFYLSGQLLTEDIYVANKFVKGFLRTNNVDANSRLCMASAVTAYKLAFGSDGVPCTYEDVDDADAFLFIGS
NAAVAHPVLFKRVLKRRKEEEKVIIVTVDPLETETAKRSDVFIQIKAGTDTVFLNSVLYVLHKEGWIDYRFIYNYTEGFE
EALEVAMKFPPDVAASICSVKPEDVYLVAELFAHSKKLISFWCQGLNQSLNGTMKNLALINLHLATGRLNEKGCPFSLTG
QPNAMGGREVGYLTNGLPGYRDVRNKADRAFMEKFWGVKGIKKEPGPTITEAIDMILKDRIKFLWVVCTNPAVSLPNLEK
VKRALEKVFLVVQDAYWNDTCEFANLILPAAQLGEKEGVMTGSDRTVTFCERFSEPFGESKPDWLIFTELARKMGAEELF
PYSSPKEIFEEFKASTKGRLCDMSNLSYENLPARWGKRWLYEDLKFPTESGKARFHPARVDVAAEEGMFILLTGRLKNNW
HTMTRTGKSPELLKGEIPPFVIMNPRDAEELGIEEGEEVILSAKGKEITRVVRFGNVKRGHIFAPFGYPSEYGEPTNLLT
SDRTDPYSKEPDLKYSGVDVFVKVNR

Sequences:

>Translated_666_residues
MKRFQCPYCGVGCGLYINEKGKVKGDFEHPANLGDICKKPLYLPKVLNRGRIAKPMYRENKSHSFREISWEEAYEILKEK
LISFSPDETYFYLSGQLLTEDIYVANKFVKGFLRTNNVDANSRLCMASAVTAYKLAFGSDGVPCTYEDVDDADAFLFIGS
NAAVAHPVLFKRVLKRRKEEEKVIIVTVDPLETETAKRSDVFIQIKAGTDTVFLNSVLYVLHKEGWIDYRFIYNYTEGFE
EALEVAMKFPPDVAASICSVKPEDVYLVAELFAHSKKLISFWCQGLNQSLNGTMKNLALINLHLATGRLNEKGCPFSLTG
QPNAMGGREVGYLTNGLPGYRDVRNKADRAFMEKFWGVKGIKKEPGPTITEAIDMILKDRIKFLWVVCTNPAVSLPNLEK
VKRALEKVFLVVQDAYWNDTCEFANLILPAAQLGEKEGVMTGSDRTVTFCERFSEPFGESKPDWLIFTELARKMGAEELF
PYSSPKEIFEEFKASTKGRLCDMSNLSYENLPARWGKRWLYEDLKFPTESGKARFHPARVDVAAEEGMFILLTGRLKNNW
HTMTRTGKSPELLKGEIPPFVIMNPRDAEELGIEEGEEVILSAKGKEITRVVRFGNVKRGHIFAPFGYPSEYGEPTNLLT
SDRTDPYSKEPDLKYSGVDVFVKVNR
>Mature_666_residues
MKRFQCPYCGVGCGLYINEKGKVKGDFEHPANLGDICKKPLYLPKVLNRGRIAKPMYRENKSHSFREISWEEAYEILKEK
LISFSPDETYFYLSGQLLTEDIYVANKFVKGFLRTNNVDANSRLCMASAVTAYKLAFGSDGVPCTYEDVDDADAFLFIGS
NAAVAHPVLFKRVLKRRKEEEKVIIVTVDPLETETAKRSDVFIQIKAGTDTVFLNSVLYVLHKEGWIDYRFIYNYTEGFE
EALEVAMKFPPDVAASICSVKPEDVYLVAELFAHSKKLISFWCQGLNQSLNGTMKNLALINLHLATGRLNEKGCPFSLTG
QPNAMGGREVGYLTNGLPGYRDVRNKADRAFMEKFWGVKGIKKEPGPTITEAIDMILKDRIKFLWVVCTNPAVSLPNLEK
VKRALEKVFLVVQDAYWNDTCEFANLILPAAQLGEKEGVMTGSDRTVTFCERFSEPFGESKPDWLIFTELARKMGAEELF
PYSSPKEIFEEFKASTKGRLCDMSNLSYENLPARWGKRWLYEDLKFPTESGKARFHPARVDVAAEEGMFILLTGRLKNNW
HTMTRTGKSPELLKGEIPPFVIMNPRDAEELGIEEGEEVILSAKGKEITRVVRFGNVKRGHIFAPFGYPSEYGEPTNLLT
SDRTDPYSKEPDLKYSGVDVFVKVNR

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=790, Percent_Identity=28.6075949367089, Blast_Score=275, Evalue=8e-75,
Organism=Escherichia coli, GI3868721, Length=693, Percent_Identity=27.994227994228, Blast_Score=260, Evalue=2e-70,
Organism=Escherichia coli, GI3868720, Length=631, Percent_Identity=24.0887480190174, Blast_Score=135, Evalue=9e-33,
Organism=Escherichia coli, GI3868719, Length=612, Percent_Identity=23.8562091503268, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI1787778, Length=400, Percent_Identity=21.25, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI87081797, Length=721, Percent_Identity=23.0235783633842, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1787231, Length=354, Percent_Identity=23.728813559322, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI171474008, Length=710, Percent_Identity=20.9859154929577, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 75404; Mature: 75404

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFQCPYCGVGCGLYINEKGKVKGDFEHPANLGDICKKPLYLPKVLNRGRIAKPMYREN
CCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHCCCCHHHHHHCCCCCCCCHHHCC
KSHSFREISWEEAYEILKEKLISFSPDETYFYLSGQLLTEDIYVANKFVKGFLRTNNVDA
CCCCHHCCCHHHHHHHHHHHHHCCCCCCEEEEEECCEEHHHHHHHHHHHHHHHHCCCCCC
NSRLCMASAVTAYKLAFGSDGVPCTYEDVDDADAFLFIGSNAAVAHPVLFKRVLKRRKEE
CCCCHHHHHHHHHEEEECCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCC
EKVIIVTVDPLETETAKRSDVFIQIKAGTDTVFLNSVLYVLHKEGWIDYRFIYNYTEGFE
CCEEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEECHHHHHH
EALEVAMKFPPDVAASICSVKPEDVYLVAELFAHSKKLISFWCQGLNQSLNGTMKNLALI
HHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHCCHHHCEEEE
NLHLATGRLNEKGCPFSLTGQPNAMGGREVGYLTNGLPGYRDVRNKADRAFMEKFWGVKG
EEEEEECCCCCCCCCEEECCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCC
IKKEPGPTITEAIDMILKDRIKFLWVVCTNPAVSLPNLEKVKRALEKVFLVVQDAYWNDT
CCCCCCCHHHHHHHHHHHHCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCH
CEFANLILPAAQLGEKEGVMTGSDRTVTFCERFSEPFGESKPDWLIFTELARKMGAEELF
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHCCHHHCC
PYSSPKEIFEEFKASTKGRLCDMSNLSYENLPARWGKRWLYEDLKFPTESGKARFHPARV
CCCCHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEECCCEE
DVAAEEGMFILLTGRLKNNWHTMTRTGKSPELLKGEIPPFVIMNPRDAEELGIEEGEEVI
EEECCCCEEEEEECCCCCCCEEEECCCCCCHHHCCCCCCEEEECCCCHHHCCCCCCCEEE
LSAKGKEITRVVRFGNVKRGHIFAPFGYPSEYGEPTNLLTSDRTDPYSKEPDLKYSGVDV
EECCCHHHHHHHHHCCCCCCCEEECCCCCHHCCCCCCCCCCCCCCCCCCCCCCEECCEEE
FVKVNR
EEEECC
>Mature Secondary Structure
MKRFQCPYCGVGCGLYINEKGKVKGDFEHPANLGDICKKPLYLPKVLNRGRIAKPMYREN
CCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHCCCCHHHHHHCCCCCCCCHHHCC
KSHSFREISWEEAYEILKEKLISFSPDETYFYLSGQLLTEDIYVANKFVKGFLRTNNVDA
CCCCHHCCCHHHHHHHHHHHHHCCCCCCEEEEEECCEEHHHHHHHHHHHHHHHHCCCCCC
NSRLCMASAVTAYKLAFGSDGVPCTYEDVDDADAFLFIGSNAAVAHPVLFKRVLKRRKEE
CCCCHHHHHHHHHEEEECCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCC
EKVIIVTVDPLETETAKRSDVFIQIKAGTDTVFLNSVLYVLHKEGWIDYRFIYNYTEGFE
CCEEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEECHHHHHH
EALEVAMKFPPDVAASICSVKPEDVYLVAELFAHSKKLISFWCQGLNQSLNGTMKNLALI
HHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHCCHHHCEEEE
NLHLATGRLNEKGCPFSLTGQPNAMGGREVGYLTNGLPGYRDVRNKADRAFMEKFWGVKG
EEEEEECCCCCCCCCEEECCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCC
IKKEPGPTITEAIDMILKDRIKFLWVVCTNPAVSLPNLEKVKRALEKVFLVVQDAYWNDT
CCCCCCCHHHHHHHHHHHHCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCH
CEFANLILPAAQLGEKEGVMTGSDRTVTFCERFSEPFGESKPDWLIFTELARKMGAEELF
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHCCHHHCC
PYSSPKEIFEEFKASTKGRLCDMSNLSYENLPARWGKRWLYEDLKFPTESGKARFHPARV
CCCCHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEECCCEE
DVAAEEGMFILLTGRLKNNWHTMTRTGKSPELLKGEIPPFVIMNPRDAEELGIEEGEEVI
EEECCCCEEEEEECCCCCCCEEEECCCCCCHHHCCCCCCEEEECCCCHHHCCCCCCCEEE
LSAKGKEITRVVRFGNVKRGHIFAPFGYPSEYGEPTNLLTSDRTDPYSKEPDLKYSGVDV
EECCCHHHHHHHHHCCCCCCCEEECCCCCHHCCCCCCCCCCCCCCCCCCCCCCEECCEEE
FVKVNR
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]