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The map label for this gene is folK

Identifier: 15605733

GI number: 15605733

Start: 98536

End: 99018

Strand: Direct

Name: folK

Synonym: aq_162

Alternate gene names: 15605733

Gene position: 98536-99018 (Clockwise)

Preceding gene: 15605731

Following gene: 15605734

Centisome position: 6.35

GC content: 38.51

Gene sequence:

>483_bases
ATGGCTACAGTCTATTTAGGACTCGGGAGCAACGTAGGAGATAGGATATCTTACATACTTAAAGCGATAGAAAAACTTGA
GGAATTTTTGGAAATAGAAAAAATATCCACAGTCTACGAAAGCAAAGCCTGGGGTTTTGAAAACCAAGGAAACTTTCTAA
ATTTCGTTTTAAAGGCAAAGACTTCTCTTCTCCCGCAGGAACTTCTTTTGAAGATAAAAAAAGTGGAAAAGGAAGTTGGA
CGAAAGGAAAGGTTTAAGTGGGGCCCGAGGGAAATAGACATAGATATACTCCTGTACAAAGATGAAGTCATCAGGACCAA
ACTCCTGAAAGTCCCCCATCCCTTTCTGGAAAAAAGGGATTTTTTCGTCTATCCACTACTTGAAATAGAACCGAATGTAA
TACATCCTATCTACAGAAAGCCTCTGAAGGAGTTTAAGCCCGAAAATACACTGAAACCTTTCTGCTGTATCTTAAAAGTA
TGA

Upstream 100 bases:

>100_bases
CTAACCTCTCCTTAGAATATCTTAAAACAAATTTTAAGATAGAATTATTGAACGTAAAGTCCAGTAAGCTTTATCAGGTG
CAATAAAGGAAAGTTCAAGT

Downstream 100 bases:

>100_bases
AGCGTGTTCTCGTTGTGGACGACGAAGAAAGTATTACTTCTTCTCTCTCCGCAATCCTTGAAGAAGAAGGATACCATCCA
GATACGGCAAAGACGTTAAG

Product: folate biosynthesis 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MATVYLGLGSNVGDRISYILKAIEKLEEFLEIEKISTVYESKAWGFENQGNFLNFVLKAKTSLLPQELLLKIKKVEKEVG
RKERFKWGPREIDIDILLYKDEVIRTKLLKVPHPFLEKRDFFVYPLLEIEPNVIHPIYRKPLKEFKPENTLKPFCCILKV

Sequences:

>Translated_160_residues
MATVYLGLGSNVGDRISYILKAIEKLEEFLEIEKISTVYESKAWGFENQGNFLNFVLKAKTSLLPQELLLKIKKVEKEVG
RKERFKWGPREIDIDILLYKDEVIRTKLLKVPHPFLEKRDFFVYPLLEIEPNVIHPIYRKPLKEFKPENTLKPFCCILKV
>Mature_159_residues
ATVYLGLGSNVGDRISYILKAIEKLEEFLEIEKISTVYESKAWGFENQGNFLNFVLKAKTSLLPQELLLKIKKVEKEVGR
KERFKWGPREIDIDILLYKDEVIRTKLLKVPHPFLEKRDFFVYPLLEIEPNVIHPIYRKPLKEFKPENTLKPFCCILKV

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family

Homologues:

Organism=Escherichia coli, GI1786335, Length=139, Percent_Identity=35.2517985611511, Blast_Score=99, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324073, Length=148, Percent_Identity=35.8108108108108, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HPPK_AQUAE (O66550)

Other databases:

- EMBL:   AE000657
- PIR:   C70315
- RefSeq:   NP_213110.1
- ProteinModelPortal:   O66550
- SMR:   O66550
- GeneID:   1192693
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_162
- NMPDR:   fig|224324.1.peg.125
- HOGENOM:   HBG662565
- OMA:   ETDPVGY
- PhylomeDB:   O66550
- ProtClustDB:   CLSK2299313
- BioCyc:   AAEO224324:AQ_162-MONOMER
- BRENDA:   2.7.6.3
- InterPro:   IPR000550
- Gene3D:   G3DSA:3.30.70.560
- TIGRFAMs:   TIGR01498

Pfam domain/function: PF01288 HPPK; SSF55083 Hppk

EC number: =2.7.6.3

Molecular weight: Translated: 18839; Mature: 18707

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATVYLGLGSNVGDRISYILKAIEKLEEFLEIEKISTVYESKAWGFENQGNFLNFVLKAK
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
TSLLPQELLLKIKKVEKEVGRKERFKWGPREIDIDILLYKDEVIRTKLLKVPHPFLEKRD
HHCCHHHHHHHHHHHHHHHCHHHHCCCCCCEEEEEEEEECHHHHHHHHHHCCCHHHHCCC
FFVYPLLEIEPNVIHPIYRKPLKEFKPENTLKPFCCILKV
EEEEEEEECCCCEECHHHHHHHHHCCCCCCCCHHHHEECC
>Mature Secondary Structure 
ATVYLGLGSNVGDRISYILKAIEKLEEFLEIEKISTVYESKAWGFENQGNFLNFVLKAK
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
TSLLPQELLLKIKKVEKEVGRKERFKWGPREIDIDILLYKDEVIRTKLLKVPHPFLEKRD
HHCCHHHHHHHHHHHHHHHCHHHHCCCCCCEEEEEEEEECHHHHHHHHHHCCCHHHHCCC
FFVYPLLEIEPNVIHPIYRKPLKEFKPENTLKPFCCILKV
EEEEEEEECCCCEECHHHHHHHHHCCCCCCCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320