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The map label for this gene is apfA [H]

Identifier: 15605731

GI number: 15605731

Start: 96893

End: 97297

Strand: Direct

Name: apfA [H]

Synonym: aq_158

Alternate gene names: 15605731

Gene position: 96893-97297 (Clockwise)

Preceding gene: 15605730

Following gene: 15605733

Centisome position: 6.25

GC content: 42.72

Gene sequence:

>405_bases
ATGAAGAAGGAGTTCTCCGCAGGGGGAGTGCTTTTCAAAGATGGAGAAGTGCTCCTCATAAAGACTCCCTCAAACGTATG
GAGTTTTCCAAAGGGAAACATAGAGCCGGGAGAAAAACCTGAGGAAACGGCGGTAAGGGAAGTGTGGGAGGAAACAGGAG
TAAAGGGAGAGATTCTGGATTACATAGGCGAAATCCACTACTGGTATACGCTAAAGGGGGAGCGGATTTTCAAAACCGTA
AAGTACTACTTGATGAAGTATAAAGAGGGAGAGCCGAGACCCTCATGGGAAGTGAAAGACGCCAAGTTTTTTCCAATTAA
AGAGGCTAAAAAGTTATTAAAGTACAAAGGAGACAAGGAAATCTTTGAAAAGGCTCTAAAACTCAAGGAAAAGTTCAAAC
TTTAA

Upstream 100 bases:

>100_bases
CTAAGAAGAAAAAGATAAAGGCCGTTTTTGACTGCAACTCCATGCTCTACTGGGATAAGAAAATAGACATCACAAATGAA
GTTCTGAAAGAACTCGATAA

Downstream 100 bases:

>100_bases
CCGTCCCTTTTTGTTCCTCTTCTATCCTCTCAAGTGTAGGATCATTTTTCTTTCCAGTAAGGATGTCCAGTAGTTCCTTT
ACGTGTATTATTCCCTGATA

Product: AP4A hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 134; Mature: 134

Protein sequence:

>134_residues
MKKEFSAGGVLFKDGEVLLIKTPSNVWSFPKGNIEPGEKPEETAVREVWEETGVKGEILDYIGEIHYWYTLKGERIFKTV
KYYLMKYKEGEPRPSWEVKDAKFFPIKEAKKLLKYKGDKEIFEKALKLKEKFKL

Sequences:

>Translated_134_residues
MKKEFSAGGVLFKDGEVLLIKTPSNVWSFPKGNIEPGEKPEETAVREVWEETGVKGEILDYIGEIHYWYTLKGERIFKTV
KYYLMKYKEGEPRPSWEVKDAKFFPIKEAKKLLKYKGDKEIFEKALKLKEKFKL
>Mature_134_residues
MKKEFSAGGVLFKDGEVLLIKTPSNVWSFPKGNIEPGEKPEETAVREVWEETGVKGEILDYIGEIHYWYTLKGERIFKTV
KYYLMKYKEGEPRPSWEVKDAKFFPIKEAKKLLKYKGDKEIFEKALKLKEKFKL

Specific function: May be involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8- oxoguanine, 8-oxo-dGTP) from DNA and the nucleotide pool. In vitro has dATPase rather than 8-oxo-dGTPase activity [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.-.- [C]

Molecular weight: Translated: 15757; Mature: 15757

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEFSAGGVLFKDGEVLLIKTPSNVWSFPKGNIEPGEKPEETAVREVWEETGVKGEILD
CCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHH
YIGEIHYWYTLKGERIFKTVKYYLMKYKEGEPRPSWEVKDAKFFPIKEAKKLLKYKGDKE
HHCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCHHHHHHHHHCCCCHH
IFEKALKLKEKFKL
HHHHHHHHHHHHCC
>Mature Secondary Structure
MKKEFSAGGVLFKDGEVLLIKTPSNVWSFPKGNIEPGEKPEETAVREVWEETGVKGEILD
CCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHH
YIGEIHYWYTLKGERIFKTVKYYLMKYKEGEPRPSWEVKDAKFFPIKEAKKLLKYKGDKE
HHCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCHHHHHHHHHCCCCHH
IFEKALKLKEKFKL
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]