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The map label for this gene is glnB

Identifier: 15605696

GI number: 15605696

Start: 67339

End: 67677

Strand: Direct

Name: glnB

Synonym: aq_109

Alternate gene names: 15605696

Gene position: 67339-67677 (Clockwise)

Preceding gene: 15605694

Following gene: 15605697

Centisome position: 4.34

GC content: 46.31

Gene sequence:

>339_bases
ATGAAAAAGATCGAGGCGATTATTAAACCTTTTAAGCTCGACGAAGTGAAGGACGCCCTCGTTGAGATAGGCATAGGCGG
TATGACCGTAACCGAGGTAAAGGGTTTTGGTCAGCAGAAGGGCCACACGGAGATTTACAGGGGAACTGAGTACGTAATTG
ACTTTCTGCCAAAGGTAAAGATTGAAGTTGTCGTGAGAGACGAAGACGTGGAAAAGGTGGTGGAAACCATTGTGAAAACT
GCCCAGACGGGAAGGGTAGGGGACGGAAAGATATTCATAATTCCTGTTGAGGATGTCATAAGGATCAGGACCGGTGAAAG
AGGTGAACAAGCCATTTAA

Upstream 100 bases:

>100_bases
GTTAACAAAATTGTAAACACGAAAAATTTCCATACCTTTGTAAAAGCGTATTCTGAAATTGGCACGGAAATTGCATAATA
ACTTACAAGGAGGAAGGAAG

Downstream 100 bases:

>100_bases
AAATAAAAGGAGGTAGTAGTATGCCAAAGTACACACCAGAAGAAGTCTTAGACCTTATTCAGAAGGAAGGGGTTCAGTAC
GTTGACCTGAGGTTTTCTGA

Product: nitrogen regulatory PII protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 112; Mature: 112

Protein sequence:

>112_residues
MKKIEAIIKPFKLDEVKDALVEIGIGGMTVTEVKGFGQQKGHTEIYRGTEYVIDFLPKVKIEVVVRDEDVEKVVETIVKT
AQTGRVGDGKIFIIPVEDVIRIRTGERGEQAI

Sequences:

>Translated_112_residues
MKKIEAIIKPFKLDEVKDALVEIGIGGMTVTEVKGFGQQKGHTEIYRGTEYVIDFLPKVKIEVVVRDEDVEKVVETIVKT
AQTGRVGDGKIFIIPVEDVIRIRTGERGEQAI
>Mature_112_residues
MKKIEAIIKPFKLDEVKDALVEIGIGGMTVTEVKGFGQQKGHTEIYRGTEYVIDFLPKVKIEVVVRDEDVEKVVETIVKT
AQTGRVGDGKIFIIPVEDVIRIRTGERGEQAI

Specific function: In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P- II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deur

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family

Homologues:

Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=70.5357142857143, Blast_Score=161, Evalue=9e-42,
Organism=Escherichia coli, GI1786655, Length=112, Percent_Identity=58.0357142857143, Blast_Score=139, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLNB_AQUAE (O66513)

Other databases:

- EMBL:   AE000657
- PIR:   F70310
- RefSeq:   NP_213073.1
- PDB:   2EG1
- PDB:   2EG2
- PDB:   2Z0G
- PDBsum:   2EG1
- PDBsum:   2EG2
- PDBsum:   2Z0G
- ProteinModelPortal:   O66513
- SMR:   O66513
- GeneID:   1192524
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_109
- NMPDR:   fig|224324.1.peg.88
- HOGENOM:   HBG748089
- OMA:   MDDVREA
- PhylomeDB:   O66513
- ProtClustDB:   CLSK2299290
- BioCyc:   AAEO224324:AQ_109-MONOMER
- GO:   GO:0006350
- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- Gene3D:   G3DSA:3.30.70.120
- PRINTS:   PR00340
- SMART:   SM00938

Pfam domain/function: PF00543 P-II; SSF54913 N-reg_PII-like_a/b

EC number: NA

Molecular weight: Translated: 12497; Mature: 12497

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00638 PII_GLNB_CTER; PS51343 PII_GLNB_DOM; PS00496 PII_GLNB_UMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIEAIIKPFKLDEVKDALVEIGIGGMTVTEVKGFGQQKGHTEIYRGTEYVIDFLPKVK
CCCHHHHHCCCCHHHHHHHHHHHCCCCEEEEHHCCCCCCCCCCHHHCCHHHHHHHCCCCE
IEVVVRDEDVEKVVETIVKTAQTGRVGDGKIFIIPVEDVIRIRTGERGEQAI
EEEEEECCCHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHEEECCCCCCCCCC
>Mature Secondary Structure
MKKIEAIIKPFKLDEVKDALVEIGIGGMTVTEVKGFGQQKGHTEIYRGTEYVIDFLPKVK
CCCHHHHHCCCCHHHHHHHHHHHCCCCEEEEHHCCCCCCCCCCHHHCCHHHHHHHCCCCE
IEVVVRDEDVEKVVETIVKTAQTGRVGDGKIFIIPVEDVIRIRTGERGEQAI
EEEEEECCCHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320