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The map label for this gene is glnA

Identifier: 15605697

GI number: 15605697

Start: 67698

End: 69107

Strand: Direct

Name: glnA

Synonym: aq_111

Alternate gene names: 15605697

Gene position: 67698-69107 (Clockwise)

Preceding gene: 15605696

Following gene: 15605698

Centisome position: 4.36

GC content: 47.09

Gene sequence:

>1410_bases
ATGCCAAAGTACACACCAGAAGAAGTCTTAGACCTTATTCAGAAGGAAGGGGTTCAGTACGTTGACCTGAGGTTTTCTGA
CCCCTTCGGTCAGTGGCAGCACTTAACGATCCCCGCCTACGAAATATCTAAGGAAACTTTTGAAGTAGGCAGGGGATTTG
ACGGGTCTTCCATCAGGGGATGGCAGTCCATCAACGAGTCTGACATGCTCGCAAAACCTGACCCCAATACCGCGTTTATA
GACCCATTTATAGAGCCAAAGACGCTCGTAATGATTTGTGATATCTACGATCCCGTTACGGGTGAAAGGTATGGAAGGGA
CACCAGGTACATAGCACAAAAAGCTGAACAGTACCTCAAGCAAACTGGAATAGGAGATACCGCGTACTTTGGACCCGAAG
CGGAGTTCTTCATATTTGACAGTGTGGAGTTTGGCACCGCTGCTAATTACGCCTTCTGGAGAGTAGACTCAGAAGAAGGA
TGGTGGAACAGAGAAGTACCCTCTTCCGGATATAAGATACCCCACAAGAGAGGATACTTCCCCGCACCGCCCGTTGACAA
GATGATGCAACTGAGAAACGAAATGGTTTCTATAATGTCGGACCTCGGTATAATAGTTGAGCTCCACCACCACGAAGTTG
CAACAGCTGGACAGGGCGAAATAGACATAAGGTACGACAGCCTTTTAAATCAGGCGGATAAACTCTTCCTCTACAAGTAC
ATAGTTAGAATGGTTGCCGCAAAACACGGAAAGTACGCAACCTTTATGGCTAAGGTTCTTCCAAACGACAACGGTTCTGG
AATGCACACCCACTTCTCCATATGGAAAAACGGAGAAAACCTCTTTGCAGGAAGCGAGTACGCTGGACTTTCAAAGACAG
CACTCTACGCCATAGGAGGTATACTCAAACACGGTCCCGCAATAGCGGCGTTTACAAACCCCACAGTTAACTCTTACCAC
AGGCTCGTACCCGGATACGAAGCACCCGTAAGATTAGCCTACTCCGCTAGGAACAGATCTGCGGCTATAAGAATACCCAT
GTACTCTCAAAATCCGAAGGCAAAGAGGATAGAAGTAAGGTTCCCGGACGCTACATCCAACCCTTACCTTGCGTTTGCGG
CTATACTCATGGCTGCAATAGATGGAATTGAGAACGAAATAGACCCTGGTGAACCTTTTGACAAGGACATATACTCCCTA
CCTCCCGAAGAGCTCGAAGGAATTCCTCAACTACCCGGTTCCCTTGAAGAAGCCTTAAAGGCTCTGGAGGAAGATTACGA
GTTCCTCTTAAAAGGAAACGTGTTCACAGAAGAGTTCATACAGCTCTGGATAGAGTCCAAGAGAGCCGAAATAGACGAAC
TCAGGTTTATACCCCACCCCAAAGAATTTGAACTTTACTGGGACATTTAA

Upstream 100 bases:

>100_bases
GGGACGGAAAGATATTCATAATTCCTGTTGAGGATGTCATAAGGATCAGGACCGGTGAAAGAGGTGAACAAGCCATTTAA
AAATAAAAGGAGGTAGTAGT

Downstream 100 bases:

>100_bases
ACAAATTCTCGGGGGCGTTTCGCCCCTTTTATAAAAACTTTTTTAAAAAAGGAGGGCAAAAATGAGAGCGCTAGGATTTA
TAGGGATTATTCTATCAATT

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MPKYTPEEVLDLIQKEGVQYVDLRFSDPFGQWQHLTIPAYEISKETFEVGRGFDGSSIRGWQSINESDMLAKPDPNTAFI
DPFIEPKTLVMICDIYDPVTGERYGRDTRYIAQKAEQYLKQTGIGDTAYFGPEAEFFIFDSVEFGTAANYAFWRVDSEEG
WWNREVPSSGYKIPHKRGYFPAPPVDKMMQLRNEMVSIMSDLGIIVELHHHEVATAGQGEIDIRYDSLLNQADKLFLYKY
IVRMVAAKHGKYATFMAKVLPNDNGSGMHTHFSIWKNGENLFAGSEYAGLSKTALYAIGGILKHGPAIAAFTNPTVNSYH
RLVPGYEAPVRLAYSARNRSAAIRIPMYSQNPKAKRIEVRFPDATSNPYLAFAAILMAAIDGIENEIDPGEPFDKDIYSL
PPEELEGIPQLPGSLEEALKALEEDYEFLLKGNVFTEEFIQLWIESKRAEIDELRFIPHPKEFELYWDI

Sequences:

>Translated_469_residues
MPKYTPEEVLDLIQKEGVQYVDLRFSDPFGQWQHLTIPAYEISKETFEVGRGFDGSSIRGWQSINESDMLAKPDPNTAFI
DPFIEPKTLVMICDIYDPVTGERYGRDTRYIAQKAEQYLKQTGIGDTAYFGPEAEFFIFDSVEFGTAANYAFWRVDSEEG
WWNREVPSSGYKIPHKRGYFPAPPVDKMMQLRNEMVSIMSDLGIIVELHHHEVATAGQGEIDIRYDSLLNQADKLFLYKY
IVRMVAAKHGKYATFMAKVLPNDNGSGMHTHFSIWKNGENLFAGSEYAGLSKTALYAIGGILKHGPAIAAFTNPTVNSYH
RLVPGYEAPVRLAYSARNRSAAIRIPMYSQNPKAKRIEVRFPDATSNPYLAFAAILMAAIDGIENEIDPGEPFDKDIYSL
PPEELEGIPQLPGSLEEALKALEEDYEFLLKGNVFTEEFIQLWIESKRAEIDELRFIPHPKEFELYWDI
>Mature_468_residues
PKYTPEEVLDLIQKEGVQYVDLRFSDPFGQWQHLTIPAYEISKETFEVGRGFDGSSIRGWQSINESDMLAKPDPNTAFID
PFIEPKTLVMICDIYDPVTGERYGRDTRYIAQKAEQYLKQTGIGDTAYFGPEAEFFIFDSVEFGTAANYAFWRVDSEEGW
WNREVPSSGYKIPHKRGYFPAPPVDKMMQLRNEMVSIMSDLGIIVELHHHEVATAGQGEIDIRYDSLLNQADKLFLYKYI
VRMVAAKHGKYATFMAKVLPNDNGSGMHTHFSIWKNGENLFAGSEYAGLSKTALYAIGGILKHGPAIAAFTNPTVNSYHR
LVPGYEAPVRLAYSARNRSAAIRIPMYSQNPKAKRIEVRFPDATSNPYLAFAAILMAAIDGIENEIDPGEPFDKDIYSLP
PEELEGIPQLPGSLEEALKALEEDYEFLLKGNVFTEEFIQLWIESKRAEIDELRFIPHPKEFELYWDI

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family

Homologues:

Organism=Homo sapiens, GI221307485, Length=473, Percent_Identity=24.7357293868922, Blast_Score=96, Evalue=9e-20,
Organism=Escherichia coli, GI1790301, Length=469, Percent_Identity=55.6503198294243, Blast_Score=540, Evalue=1e-155,
Organism=Escherichia coli, GI87081870, Length=472, Percent_Identity=27.7542372881356, Blast_Score=118, Evalue=7e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): GLNA_AQUAE (O66514)

Other databases:

- EMBL:   AE000657
- PIR:   G70310
- RefSeq:   NP_213074.1
- ProteinModelPortal:   O66514
- SMR:   O66514
- GeneID:   1192525
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_111
- NMPDR:   fig|224324.1.peg.89
- HOGENOM:   HBG553755
- OMA:   TWIDYKR
- PhylomeDB:   O66514
- ProtClustDB:   CLSK2299291
- BioCyc:   AAEO224324:AQ_111-MONOMER
- BRENDA:   6.3.1.2
- GO:   GO:0005737
- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- Gene3D:   G3DSA:3.30.590.10
- TIGRFAMs:   TIGR00653

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N; SSF54368 Gln_synt_beta

EC number: =6.3.1.2

Molecular weight: Translated: 53295; Mature: 53164

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00180 GLNA_1; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKYTPEEVLDLIQKEGVQYVDLRFSDPFGQWQHLTIPAYEISKETFEVGRGFDGSSIRG
CCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEECHHHHHHHHHHHCCCCCCCCCCC
WQSINESDMLAKPDPNTAFIDPFIEPKTLVMICDIYDPVTGERYGRDTRYIAQKAEQYLK
HHCCCCCCCCCCCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
QTGIGDTAYFGPEAEFFIFDSVEFGTAANYAFWRVDSEEGWWNREVPSSGYKIPHKRGYF
HCCCCCCEEECCCCCEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
PAPPVDKMMQLRNEMVSIMSDLGIIVELHHHEVATAGQGEIDIRYDSLLNQADKLFLYKY
CCCCHHHHHHHHHHHHHHHHCCCEEEEEECCEEECCCCCCEEEEHHHHHHHHHHHHHHHH
IVRMVAAKHGKYATFMAKVLPNDNGSGMHTHFSIWKNGENLFAGSEYAGLSKTALYAIGG
HHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEEEEEECCCCEECCCCCCCCHHHHHHHHHH
ILKHGPAIAAFTNPTVNSYHRLVPGYEAPVRLAYSARNRSAAIRIPMYSQNPKAKRIEVR
HHHCCCEEEEECCCCHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCCEEEEE
FPDATSNPYLAFAAILMAAIDGIENEIDPGEPFDKDIYSLPPEELEGIPQLPGSLEEALK
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHHCCCCCCCCHHHHHHH
ALEEDYEFLLKGNVFTEEFIQLWIESKRAEIDELRFIPHPKEFELYWDI
HHHHHHHEECCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCEEEEECC
>Mature Secondary Structure 
PKYTPEEVLDLIQKEGVQYVDLRFSDPFGQWQHLTIPAYEISKETFEVGRGFDGSSIRG
CCCCHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEECHHHHHHHHHHHCCCCCCCCCCC
WQSINESDMLAKPDPNTAFIDPFIEPKTLVMICDIYDPVTGERYGRDTRYIAQKAEQYLK
HHCCCCCCCCCCCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
QTGIGDTAYFGPEAEFFIFDSVEFGTAANYAFWRVDSEEGWWNREVPSSGYKIPHKRGYF
HCCCCCCEEECCCCCEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
PAPPVDKMMQLRNEMVSIMSDLGIIVELHHHEVATAGQGEIDIRYDSLLNQADKLFLYKY
CCCCHHHHHHHHHHHHHHHHCCCEEEEEECCEEECCCCCCEEEEHHHHHHHHHHHHHHHH
IVRMVAAKHGKYATFMAKVLPNDNGSGMHTHFSIWKNGENLFAGSEYAGLSKTALYAIGG
HHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEEEEEECCCCEECCCCCCCCHHHHHHHHHH
ILKHGPAIAAFTNPTVNSYHRLVPGYEAPVRLAYSARNRSAAIRIPMYSQNPKAKRIEVR
HHHCCCEEEEECCCCHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCCEEEEE
FPDATSNPYLAFAAILMAAIDGIENEIDPGEPFDKDIYSLPPEELEGIPQLPGSLEEALK
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHHCCCCCCCCHHHHHHH
ALEEDYEFLLKGNVFTEEFIQLWIESKRAEIDELRFIPHPKEFELYWDI
HHHHHHHEECCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320