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The map label for this gene is speE

Identifier: 15605658

GI number: 15605658

Start: 39496

End: 40338

Strand: Direct

Name: speE

Synonym: aq_062

Alternate gene names: 15605658

Gene position: 39496-40338 (Clockwise)

Preceding gene: 15605656

Following gene: 15605659

Centisome position: 2.55

GC content: 46.86

Gene sequence:

>843_bases
ATGAGGGACATTGTTTTCATAGAAAGGGATCCTTACGCTCCGATAAGACACTGCTACACCGTTTCAAAAATACTCTACCA
AGGAAAGAGTCCCTACCAGGAAATACAGGTAATTGAAAGTCCTGAGTTCGGGAGAATGCTTATCCTCGACGGAGTGGTTC
AGCTGGACGAGAAGTACGAATTTCTCTACCACGAGTACCTCGCACACGTTCCACTCCACGCACACCCAAACCCCAGAAAC
GTGCTCATAATCGGAGGAGGAGACGGGGGAACATTGAGGGAAGTTCTTAAACACGATATCGTAGAAAGAGCCGTCCTCGT
TGACATAGATAAAGAAGTAATAGAAGTTTCCAAGAAGTACCTCCCCACGCTATCGGTTGGTTTTCAGGACCCGAGAGCCA
TAGTCGTGAACGAAGACGGCTACAAGTATGTCCAAGATTACGAGAACGAGTTTGATGTAATCATCGTAGACTCAACGGAT
CCCGTCGGATTTGCCCACGTCCTCACCACAGAGGACTTCTTCAGACACGTCTACAAGGCGCTAAAGGAAGACGGAATATT
CGTGGCTCAAACGGAGTCCATCCACTACCACCTTGAAATGGTGAGTAACATACAACAAAGACTAAAGAAAGTGTTTCCTA
TTGTTGACCTGTACACTTCCGTAATTCCCATATACGCCGGTTACTGGTGGACCTTCTCAATAGCTTCCAAGAAGTATCCT
GTGAGGACTCCCGCTAGGGAGGTAAAGGTCCAAACGAAGATTTACGACGCGGACATGCACGAGTACGCCTTCCTTCCCGA
GAGCTTTTACAACAAGATTGTAAACGGAGAGTACAAGTATTAA

Upstream 100 bases:

>100_bases
AAATACCTTTGCTCGCCCTGTCGGAAATCGTCACAACTCCTATAACCGCCTTCTTTTCGCTCATGGGAATACATTTTAAC
GTTTAGGTTAGAATAGTGAT

Downstream 100 bases:

>100_bases
AATAAATCTTTCAACCTCGTTTCCCCGCCCTGCGGGGAGACCTTAAAAAGGCCAGGAGGTGAAGTATGCCGAGGTTGAGG
CACTACAAAACCTTAAGGTA

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase 1; PAPT 1; SPDSY 1

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MRDIVFIERDPYAPIRHCYTVSKILYQGKSPYQEIQVIESPEFGRMLILDGVVQLDEKYEFLYHEYLAHVPLHAHPNPRN
VLIIGGGDGGTLREVLKHDIVERAVLVDIDKEVIEVSKKYLPTLSVGFQDPRAIVVNEDGYKYVQDYENEFDVIIVDSTD
PVGFAHVLTTEDFFRHVYKALKEDGIFVAQTESIHYHLEMVSNIQQRLKKVFPIVDLYTSVIPIYAGYWWTFSIASKKYP
VRTPAREVKVQTKIYDADMHEYAFLPESFYNKIVNGEYKY

Sequences:

>Translated_280_residues
MRDIVFIERDPYAPIRHCYTVSKILYQGKSPYQEIQVIESPEFGRMLILDGVVQLDEKYEFLYHEYLAHVPLHAHPNPRN
VLIIGGGDGGTLREVLKHDIVERAVLVDIDKEVIEVSKKYLPTLSVGFQDPRAIVVNEDGYKYVQDYENEFDVIIVDSTD
PVGFAHVLTTEDFFRHVYKALKEDGIFVAQTESIHYHLEMVSNIQQRLKKVFPIVDLYTSVIPIYAGYWWTFSIASKKYP
VRTPAREVKVQTKIYDADMHEYAFLPESFYNKIVNGEYKY
>Mature_280_residues
MRDIVFIERDPYAPIRHCYTVSKILYQGKSPYQEIQVIESPEFGRMLILDGVVQLDEKYEFLYHEYLAHVPLHAHPNPRN
VLIIGGGDGGTLREVLKHDIVERAVLVDIDKEVIEVSKKYLPTLSVGFQDPRAIVVNEDGYKYVQDYENEFDVIIVDSTD
PVGFAHVLTTEDFFRHVYKALKEDGIFVAQTESIHYHLEMVSNIQQRLKKVFPIVDLYTSVIPIYAGYWWTFSIASKKYP
VRTPAREVKVQTKIYDADMHEYAFLPESFYNKIVNGEYKY

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=263, Percent_Identity=40.3041825095057, Blast_Score=210, Evalue=1e-54,
Organism=Escherichia coli, GI1786312, Length=267, Percent_Identity=36.7041198501873, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI25154106, Length=262, Percent_Identity=42.3664122137405, Blast_Score=197, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI212645616, Length=217, Percent_Identity=43.3179723502304, Blast_Score=181, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6325326, Length=262, Percent_Identity=39.3129770992366, Blast_Score=206, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6323175, Length=269, Percent_Identity=38.2899628252788, Blast_Score=186, Evalue=4e-48,
Organism=Drosophila melanogaster, GI24645443, Length=250, Percent_Identity=38.4, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI21355941, Length=204, Percent_Identity=37.7450980392157, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI21358269, Length=232, Percent_Identity=27.1551724137931, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24663238, Length=232, Percent_Identity=27.1551724137931, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE1_AQUAE (O66473)

Other databases:

- EMBL:   AE000657
- PIR:   F70305
- RefSeq:   NP_213033.1
- ProteinModelPortal:   O66473
- SMR:   O66473
- GeneID:   1192486
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_062
- NMPDR:   fig|224324.1.peg.48
- HOGENOM:   HBG635113
- OMA:   FVAQTES
- PhylomeDB:   O66473
- ProtClustDB:   CLSK2299274
- BioCyc:   AAEO224324:AQ_062-MONOMER
- BRENDA:   2.5.1.16
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558
- TIGRFAMs:   TIGR00417

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 32595; Mature: 32595

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2; PS00228 TUBULIN_B_AUTOREG

Important sites: BINDING 33-33 BINDING 88-88 BINDING 108-108 BINDING 157-157 BINDING 160-160

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDIVFIERDPYAPIRHCYTVSKILYQGKSPYQEIQVIESPEFGRMLILDGVVQLDEKYE
CCCEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHEEECCCCCCCEEEEHHHHHHHHHHH
FLYHEYLAHVPLHAHPNPRNVLIIGGGDGGTLREVLKHDIVERAVLVDIDKEVIEVSKKY
HHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHEEECCHHHHHHHHHH
LPTLSVGFQDPRAIVVNEDGYKYVQDYENEFDVIIVDSTDPVGFAHVLTTEDFFRHVYKA
CCEEECCCCCCCEEEECCCCCHHHHHCCCCCCEEEEECCCCCCEEEEECHHHHHHHHHHH
LKEDGIFVAQTESIHYHLEMVSNIQQRLKKVFPIVDLYTSVIPIYAGYWWTFSIASKKYP
HHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
VRTPAREVKVQTKIYDADMHEYAFLPESFYNKIVNGEYKY
CCCCHHHEEEEEEEEECCCHHHCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MRDIVFIERDPYAPIRHCYTVSKILYQGKSPYQEIQVIESPEFGRMLILDGVVQLDEKYE
CCCEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHEEECCCCCCCEEEEHHHHHHHHHHH
FLYHEYLAHVPLHAHPNPRNVLIIGGGDGGTLREVLKHDIVERAVLVDIDKEVIEVSKKY
HHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHEEECCHHHHHHHHHH
LPTLSVGFQDPRAIVVNEDGYKYVQDYENEFDVIIVDSTDPVGFAHVLTTEDFFRHVYKA
CCEEECCCCCCCEEEECCCCCHHHHHCCCCCCEEEEECCCCCCEEEEECHHHHHHHHHHH
LKEDGIFVAQTESIHYHLEMVSNIQQRLKKVFPIVDLYTSVIPIYAGYWWTFSIASKKYP
HHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
VRTPAREVKVQTKIYDADMHEYAFLPESFYNKIVNGEYKY
CCCCHHHEEEEEEEEECCCHHHCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320