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The map label for this gene is truA

Identifier: 11499319

GI number: 11499319

Start: 1553521

End: 1554318

Strand: Reverse

Name: truA

Synonym: AF1730

Alternate gene names: 11499319

Gene position: 1554318-1553521 (Counterclockwise)

Preceding gene: 11499320

Following gene: 11499315

Centisome position: 71.35

GC content: 50.38

Gene sequence:

>798_bases
GTGATGAAGTTCGCGTTCAAGATAGCGTACTTCGGTGACAACTTTCACGGAAGTCAGTTTCAGCCCGACCAAAGGACGGT
TGAGGGAGAGGTAATCAACGCCCTAAGAAGGCTCGGAGTTGAGAATCCGAGGCTGAGGAGTGCCGGGAGAACTGATGCCG
GAGTTCATGCCTACGGCCAGGTCATTTCCTTCTACTCCGAGGACAAAATCTTTCCGAGAATGCTGAACGCTGAGCTTCCC
GAAGACATAACAGCATGGGCGTGGGCGAAGGTTTCCGAGGACTTCGACCCGAGAAGGGCGAAGAGCAGAGTTTACACATA
CGTTATGTACGGCTCGGACTACGACATTTCTGCGATGAGAAAGGCTGTTAAAGAGCTGATCGGCGTCCACGACTTCTCAA
ACTTCACCAAGAAATTCGGAGAGGGAGAGAGCTGCGTTAGGGAAATTATTAGCGCCGACATTAGAGCGGACAGGGAGTTC
ATAATCTTCGAGATTGAGGGGAACGCCTTTACCTGGAACATGGTTCGCTGCATAGTGACGGCAATAATGGAGATCGGAAA
GCAGCACAGAAGCATTGAGTGGTTCAGGGACCTCCTGAACCCGGAAAAGCACAAGGAGAGGGTCGAGCCCGCTCCACCTT
ACGGTCTAATCCTGAAGGATGTCAAATACGACGACGTGGAATTCGAAATCGACGATTACGCCTTTAAAACACTCCAGTCA
AGAATCGAGGACAGAATAATTTACCACGGTACGATTTTCAAGCTGTTCTCCCTTTTCAGGCAAAGCGGAATTTCCTGA

Upstream 100 bases:

>100_bases
CAGTTGGTCTTTATCAGGGGCAGAAGCAGGTTAAGGAGATACTGCAGAGGAGAATGAGCGCACTTAACGAGTTCGTTTCG
TCTCTCGTCGGGAGAGAGCT

Downstream 100 bases:

>100_bases
TTCCTGAGAATATTAAAACGGCGGAAACTTTCCTCGAACCGGGAATTGGATAGTCCCCGTACCTCACAACGATATCCCTG
TTGATGTTCTCTATCATCGC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MMKFAFKIAYFGDNFHGSQFQPDQRTVEGEVINALRRLGVENPRLRSAGRTDAGVHAYGQVISFYSEDKIFPRMLNAELP
EDITAWAWAKVSEDFDPRRAKSRVYTYVMYGSDYDISAMRKAVKELIGVHDFSNFTKKFGEGESCVREIISADIRADREF
IIFEIEGNAFTWNMVRCIVTAIMEIGKQHRSIEWFRDLLNPEKHKERVEPAPPYGLILKDVKYDDVEFEIDDYAFKTLQS
RIEDRIIYHGTIFKLFSLFRQSGIS

Sequences:

>Translated_265_residues
MMKFAFKIAYFGDNFHGSQFQPDQRTVEGEVINALRRLGVENPRLRSAGRTDAGVHAYGQVISFYSEDKIFPRMLNAELP
EDITAWAWAKVSEDFDPRRAKSRVYTYVMYGSDYDISAMRKAVKELIGVHDFSNFTKKFGEGESCVREIISADIRADREF
IIFEIEGNAFTWNMVRCIVTAIMEIGKQHRSIEWFRDLLNPEKHKERVEPAPPYGLILKDVKYDDVEFEIDDYAFKTLQS
RIEDRIIYHGTIFKLFSLFRQSGIS
>Mature_265_residues
MMKFAFKIAYFGDNFHGSQFQPDQRTVEGEVINALRRLGVENPRLRSAGRTDAGVHAYGQVISFYSEDKIFPRMLNAELP
EDITAWAWAKVSEDFDPRRAKSRVYTYVMYGSDYDISAMRKAVKELIGVHDFSNFTKKFGEGESCVREIISADIRADREF
IIFEIEGNAFTWNMVRCIVTAIMEIGKQHRSIEWFRDLLNPEKHKERVEPAPPYGLILKDVKYDDVEFEIDDYAFKTLQS
RIEDRIIYHGTIFKLFSLFRQSGIS

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI199559785, Length=276, Percent_Identity=30.0724637681159, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1788657, Length=245, Percent_Identity=31.0204081632653, Blast_Score=108, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71985046, Length=285, Percent_Identity=28.7719298245614, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321112, Length=265, Percent_Identity=29.4339622641509, Blast_Score=94, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6325044, Length=120, Percent_Identity=34.1666666666667, Blast_Score=63, Evalue=5e-11,
Organism=Drosophila melanogaster, GI19922728, Length=256, Percent_Identity=30.46875, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281362146, Length=258, Percent_Identity=24.8062015503876, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648455, Length=258, Percent_Identity=24.8062015503876, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_ARCFU (O28544)

Other databases:

- EMBL:   AE000782
- PIR:   A69466
- RefSeq:   NP_070558.1
- ProteinModelPortal:   O28544
- SMR:   O28544
- GeneID:   1484953
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF1730
- NMPDR:   fig|224325.1.peg.1719
- TIGR:   AF_1730
- HOGENOM:   HBG621020
- OMA:   KSFLWEM
- ProtClustDB:   PRK00021
- BioCyc:   AFUL224325:AF_1730-MONOMER
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 30830; Mature: 30830

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKFAFKIAYFGDNFHGSQFQPDQRTVEGEVINALRRLGVENPRLRSAGRTDAGVHAYGQ
CCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
VISFYSEDKIFPRMLNAELPEDITAWAWAKVSEDFDPRRAKSRVYTYVMYGSDYDISAMR
HHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHEEEEEEECCCCCHHHHH
KAVKELIGVHDFSNFTKKFGEGESCVREIISADIRADREFIIFEIEGNAFTWNMVRCIVT
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEEECCEEHHHHHHHHHH
AIMEIGKQHRSIEWFRDLLNPEKHKERVEPAPPYGLILKDVKYDDVEFEIDDYAFKTLQS
HHHHHHHHCCHHHHHHHHCCHHHHHHCCCCCCCCCEEEECCCCCCCEEEEHHHHHHHHHH
RIEDRIIYHGTIFKLFSLFRQSGIS
HHHHHEEEHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMKFAFKIAYFGDNFHGSQFQPDQRTVEGEVINALRRLGVENPRLRSAGRTDAGVHAYGQ
CCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
VISFYSEDKIFPRMLNAELPEDITAWAWAKVSEDFDPRRAKSRVYTYVMYGSDYDISAMR
HHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHEEEEEEECCCCCHHHHH
KAVKELIGVHDFSNFTKKFGEGESCVREIISADIRADREFIIFEIEGNAFTWNMVRCIVT
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEEECCEEHHHHHHHHHH
AIMEIGKQHRSIEWFRDLLNPEKHKERVEPAPPYGLILKDVKYDDVEFEIDDYAFKTLQS
HHHHHHHHCCHHHHHHHHCCHHHHHHCCCCCCCCCEEEECCCCCCCEEEEHHHHHHHHHH
RIEDRIIYHGTIFKLFSLFRQSGIS
HHHHHEEEHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475