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The map label for this gene is nth

Identifier: 11499282

GI number: 11499282

Start: 1516877

End: 1517506

Strand: Reverse

Name: nth

Synonym: AF1692

Alternate gene names: 11499282

Gene position: 1517506-1516877 (Counterclockwise)

Preceding gene: 11499284

Following gene: 11499281

Centisome position: 69.66

GC content: 52.86

Gene sequence:

>630_bases
GTGGACCCGATCGAGGTAATAGAGGTCATGGAGAGGGAGGCAATAAAAAGAAAGGCTCCTGTTTACCACCTGAAGGCTGA
GATTAAAACTCCCTTCCAGCACCTCGTTGCGGCGCTGCTGAGCTCAAGAACGAGGGACGAGGCGACGGTTAGAGCAGCCC
AGAACCTTTTTGCCAAGGTAAAGAAACCTGAGGATTTGCTGAAGCTCAGCGAGGAGGAGATAGCTGAGCTGATAAAGGGT
GTGGGCTTTTACAGAGTAAAGGCTAAAAGGCTTAAGGAGCTTGCAAAGAAGCTTGTTGAGGATTACAGCTCCGAGGTGCC
CTTGAGCTTTGAGGAGCTTGTAAAGCTGCCCGGCATAGGCAGGAAGTCTGCAAATGTTGTGCTGGCGTATTCGGACATCC
CTGCAATTCCCGTAGACACTCACGTTCACAGGATAGCCAACAGGCTTGGGTGGGCCAGAACGACAAAACCGGAGGAGACG
GAGGAGGTGTTGAAGAGGCTCTTTCCCTTGGAGTTCTGGGAAAAGGTGAACAGGGCGATGGTTGGCTTCGGCCAGACAGT
CTGCAAGCCTCAGAAGCCTCTCTGCGATGAGTGTCCTATTAAGGGCTGCCCGAGGGTTGGGGTGAAATGA

Upstream 100 bases:

>100_bases
CCCTCTCTTCCTCCCTGATGTCAACTCCAGCCTTGGCGTAGTCGAACTTTGGCATGATTCAAGTCGGAAAAGTCTTTTTA
AAAGGTTGCCCATCTGAAGT

Downstream 100 bases:

>100_bases
ACTACCTCGTTCTCCTAATCCCTGTCCTCTTCAGCTTCCATCAGATTTTCGTAAGGCTTGGAAGCAGGGAGGCGGAGACG
ATAAGCGGGATTTACGTTAG

Product: endonuclease III (nth)

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MDPIEVIEVMEREAIKRKAPVYHLKAEIKTPFQHLVAALLSSRTRDEATVRAAQNLFAKVKKPEDLLKLSEEEIAELIKG
VGFYRVKAKRLKELAKKLVEDYSSEVPLSFEELVKLPGIGRKSANVVLAYSDIPAIPVDTHVHRIANRLGWARTTKPEET
EEVLKRLFPLEFWEKVNRAMVGFGQTVCKPQKPLCDECPIKGCPRVGVK

Sequences:

>Translated_209_residues
MDPIEVIEVMEREAIKRKAPVYHLKAEIKTPFQHLVAALLSSRTRDEATVRAAQNLFAKVKKPEDLLKLSEEEIAELIKG
VGFYRVKAKRLKELAKKLVEDYSSEVPLSFEELVKLPGIGRKSANVVLAYSDIPAIPVDTHVHRIANRLGWARTTKPEET
EEVLKRLFPLEFWEKVNRAMVGFGQTVCKPQKPLCDECPIKGCPRVGVK
>Mature_209_residues
MDPIEVIEVMEREAIKRKAPVYHLKAEIKTPFQHLVAALLSSRTRDEATVRAAQNLFAKVKKPEDLLKLSEEEIAELIKG
VGFYRVKAKRLKELAKKLVEDYSSEVPLSFEELVKLPGIGRKSANVVLAYSDIPAIPVDTHVHRIANRLGWARTTKPEET
EEVLKRLFPLEFWEKVNRAMVGFGQTVCKPQKPLCDECPIKGCPRVGVK

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=196, Percent_Identity=39.7959183673469, Blast_Score=144, Evalue=4e-35,
Organism=Homo sapiens, GI190358497, Length=151, Percent_Identity=32.4503311258278, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI6912520, Length=151, Percent_Identity=32.4503311258278, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI115298650, Length=151, Percent_Identity=32.4503311258278, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI115298648, Length=156, Percent_Identity=32.0512820512821, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI115298654, Length=151, Percent_Identity=32.4503311258278, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI115298652, Length=151, Percent_Identity=32.4503311258278, Blast_Score=78, Evalue=7e-15,
Organism=Escherichia coli, GI1787920, Length=202, Percent_Identity=32.1782178217822, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1789331, Length=182, Percent_Identity=27.4725274725275, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17554540, Length=181, Percent_Identity=41.4364640883978, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6324530, Length=181, Percent_Identity=34.2541436464088, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319304, Length=172, Percent_Identity=34.8837209302326, Blast_Score=100, Evalue=1e-22,
Organism=Drosophila melanogaster, GI45550361, Length=215, Percent_Identity=40.4651162790698, Blast_Score=148, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23668; Mature: 23668

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPIEVIEVMEREAIKRKAPVYHLKAEIKTPFQHLVAALLSSRTRDEATVRAAQNLFAKV
CCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
KKPEDLLKLSEEEIAELIKGVGFYRVKAKRLKELAKKLVEDYSSEVPLSFEELVKLPGIG
CCCHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCC
RKSANVVLAYSDIPAIPVDTHVHRIANRLGWARTTKPEETEEVLKRLFPLEFWEKVNRAM
CCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHH
VGFGQTVCKPQKPLCDECPIKGCPRVGVK
HHCCHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDPIEVIEVMEREAIKRKAPVYHLKAEIKTPFQHLVAALLSSRTRDEATVRAAQNLFAKV
CCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
KKPEDLLKLSEEEIAELIKGVGFYRVKAKRLKELAKKLVEDYSSEVPLSFEELVKLPGIG
CCCHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCC
RKSANVVLAYSDIPAIPVDTHVHRIANRLGWARTTKPEETEEVLKRLFPLEFWEKVNRAM
CCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHH
VGFGQTVCKPQKPLCDECPIKGCPRVGVK
HHCCHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]