Click here to switch to the map view.

The map label for this gene is trpS

Identifier: 11499284

GI number: 11499284

Start: 1518538

End: 1519800

Strand: Reverse

Name: trpS

Synonym: AF1694

Alternate gene names: 11499284

Gene position: 1519800-1518538 (Counterclockwise)

Preceding gene: 11499294

Following gene: 11499282

Centisome position: 69.77

GC content: 52.73

Gene sequence:

>1263_bases
ATGAACGTTACTCCATGGGAGGTCGAGGGAGTCATAGACTACAGCAAGCTCATAGAGGAGTTCGGGATGCAGCCCTTCAG
CGAGGTGCTGCCTGAAATCGACAACCCCCACATCCTGATGCGGAGAGGGGCGATTTTCGGCCACAGGGATTATTGGAGAA
TTATCGAGGCGATGCAGAAAAAGGAGCCATGGGCGGTTATGTCAGGCTTCATGCCCTCTGGTCTGCCTCACTTCGGCCAC
AAGATGACGATGGATGAAATAGTATGGCATCAGAGCGCGGGAGGTAAAGCTTTTGTAGCAATTGCCGACATGGAGGCTCA
CTCGGTAAGGGGTTTGAGCTGGGAGAAGACGAGGGAGCTCGGAATGCTTTACATCAAATCCATCATCGCCCTCGGCCTTA
GGGAGGATGCTGTAATCTACTTCCAGTCCAAAAGCTCTCACGTAAAGGACTTGGCCTTCGAGCTTTCGGCAGAGGTGAAC
TTCAGCGAGCTGAGGGCAATTTACGGCTTCAACTCCGACACAAGCCTTGCTAAGATGTTTGTCACAGCCATTCAGGCTGC
TGACATCCTCCATCCACAGCTTTCCGATTTTGGAGGGCCAAAGCCAGTGGTGGTGCCTGTTGGGGCTGATCAGGACCCCC
ACATGCGACTGACGAGGGACTTGGCGGCGAGAATCAGCATCTTCAGCTTCGAGCCAGTTGAAGGAGGAGTGAGAGTTAGG
AGCAGGAAGGGGGCGGAGTATCTCAGCAGTTTGCGGGATTTAGAGTTCGACAAGAAAATTTACGAGGAGCACATGGACAT
CTTTGGCGAAGCTGAGGAGATTGAGAGGGCTGTGAGAAAGATAGAGGTTGAAATTGGAGGTTTTGCCTTCATTCCCCCAT
CCTCAACCTACCACCGCTTTACGACAGGGCTTACTGGTGGGAAGATGAGCTCCAGCAAGCCTGAAAGCTACATCTCCCTC
CTCGACCCTCCTGAAGAGGGAGCGAAGAAGGTCATGAAGGCCTTCACGGGTGGAAGGGCGACAGCCGAGGAGCAGAGAAG
GCTTGGCGGTGAGCCGGATAGATGTGTGGTCTTCGAGCTTTACAGCTTCCACCTCATTGATAGCGATGAGGAGCTAAACC
AGATTGAGGCAGAATGCAGGGAGGGGAGGTTGCTCTGCGGGAAGTGCAAGAAGATGGCTGCCGAGCTTGTGAAAAGCTTC
CTGAAAGAGCATCAGGAAAAAATGGAGGCAGTTGATCTTAGCAATTACACCATAATAGGCTAA

Upstream 100 bases:

>100_bases
AGGGAACCGAGAGCTCCTGCACCCATTATCTGAACCCTTTGAACTCTCATTCAGGAATAAGTCTGCACAAAGAATATTAA
CCTGCTGCTTACTGAACCAC

Downstream 100 bases:

>100_bases
GCGTCGAGCCTCAAGTCTTTCACATAAACTCCTTCTCCCTTTTCGACGTAACCTACAACCCTTCCCTCACAGAGCTTTTT
CAGCTTATCAGCTTCATCTT

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MNVTPWEVEGVIDYSKLIEEFGMQPFSEVLPEIDNPHILMRRGAIFGHRDYWRIIEAMQKKEPWAVMSGFMPSGLPHFGH
KMTMDEIVWHQSAGGKAFVAIADMEAHSVRGLSWEKTRELGMLYIKSIIALGLREDAVIYFQSKSSHVKDLAFELSAEVN
FSELRAIYGFNSDTSLAKMFVTAIQAADILHPQLSDFGGPKPVVVPVGADQDPHMRLTRDLAARISIFSFEPVEGGVRVR
SRKGAEYLSSLRDLEFDKKIYEEHMDIFGEAEEIERAVRKIEVEIGGFAFIPPSSTYHRFTTGLTGGKMSSSKPESYISL
LDPPEEGAKKVMKAFTGGRATAEEQRRLGGEPDRCVVFELYSFHLIDSDEELNQIEAECREGRLLCGKCKKMAAELVKSF
LKEHQEKMEAVDLSNYTIIG

Sequences:

>Translated_420_residues
MNVTPWEVEGVIDYSKLIEEFGMQPFSEVLPEIDNPHILMRRGAIFGHRDYWRIIEAMQKKEPWAVMSGFMPSGLPHFGH
KMTMDEIVWHQSAGGKAFVAIADMEAHSVRGLSWEKTRELGMLYIKSIIALGLREDAVIYFQSKSSHVKDLAFELSAEVN
FSELRAIYGFNSDTSLAKMFVTAIQAADILHPQLSDFGGPKPVVVPVGADQDPHMRLTRDLAARISIFSFEPVEGGVRVR
SRKGAEYLSSLRDLEFDKKIYEEHMDIFGEAEEIERAVRKIEVEIGGFAFIPPSSTYHRFTTGLTGGKMSSSKPESYISL
LDPPEEGAKKVMKAFTGGRATAEEQRRLGGEPDRCVVFELYSFHLIDSDEELNQIEAECREGRLLCGKCKKMAAELVKSF
LKEHQEKMEAVDLSNYTIIG
>Mature_420_residues
MNVTPWEVEGVIDYSKLIEEFGMQPFSEVLPEIDNPHILMRRGAIFGHRDYWRIIEAMQKKEPWAVMSGFMPSGLPHFGH
KMTMDEIVWHQSAGGKAFVAIADMEAHSVRGLSWEKTRELGMLYIKSIIALGLREDAVIYFQSKSSHVKDLAFELSAEVN
FSELRAIYGFNSDTSLAKMFVTAIQAADILHPQLSDFGGPKPVVVPVGADQDPHMRLTRDLAARISIFSFEPVEGGVRVR
SRKGAEYLSSLRDLEFDKKIYEEHMDIFGEAEEIERAVRKIEVEIGGFAFIPPSSTYHRFTTGLTGGKMSSSKPESYISL
LDPPEEGAKKVMKAFTGGRATAEEQRRLGGEPDRCVVFELYSFHLIDSDEELNQIEAECREGRLLCGKCKKMAAELVKSF
LKEHQEKMEAVDLSNYTIIG

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI47419920, Length=243, Percent_Identity=29.2181069958848, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI47419918, Length=243, Percent_Identity=29.2181069958848, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI47419916, Length=243, Percent_Identity=29.2181069958848, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI47419914, Length=243, Percent_Identity=29.2181069958848, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI72001474, Length=427, Percent_Identity=23.8875878220141, Blast_Score=101, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6324475, Length=247, Percent_Identity=27.1255060728745, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17864462, Length=242, Percent_Identity=28.5123966942149, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24645295, Length=242, Percent_Identity=28.5123966942149, Blast_Score=89, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYW_ARCFU (O28579)

Other databases:

- EMBL:   AE000782
- PIR:   E69461
- RefSeq:   NP_070522.1
- ProteinModelPortal:   O28579
- SMR:   O28579
- GeneID:   1484917
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF1694
- NMPDR:   fig|224325.1.peg.1683
- TIGR:   AF_1694
- HOGENOM:   HBG435805
- OMA:   ADILHPQ
- ProtClustDB:   PRK12285
- BioCyc:   AFUL224325:AF_1694-MONOMER
- BRENDA:   6.1.1.2
- GO:   GO:0005737
- HAMAP:   MF_00140_A
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306
- InterPro:   IPR020653
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10055
- PRINTS:   PR01039
- TIGRFAMs:   TIGR00233

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.2

Molecular weight: Translated: 47284; Mature: 47284

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVTPWEVEGVIDYSKLIEEFGMQPFSEVLPEIDNPHILMRRGAIFGHRDYWRIIEAMQK
CCCCCEEEHHHHHHHHHHHHHCCCCHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHC
KEPWAVMSGFMPSGLPHFGHKMTMDEIVWHQSAGGKAFVAIADMEAHSVRGLSWEKTREL
CCCHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEEECCCHHHCCCCCHHHHHHH
GMLYIKSIIALGLREDAVIYFQSKSSHVKDLAFELSAEVNFSELRAIYGFNSDTSLAKMF
HHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHH
VTAIQAADILHPQLSDFGGPKPVVVPVGADQDPHMRLTRDLAARISIFSFEPVEGGVRVR
HHHHHHHHHCCCHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHEEEEECCCCCCCEEE
SRKGAEYLSSLRDLEFDKKIYEEHMDIFGEAEEIERAVRKIEVEIGGFAFIPPSSTYHRF
CCCCHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEECCEEECCCCCCCHHH
TTGLTGGKMSSSKPESYISLLDPPEEGAKKVMKAFTGGRATAEEQRRLGGEPDRCVVFEL
HCCCCCCCCCCCCCHHHHEECCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEEEE
YSFHLIDSDEELNQIEAECREGRLLCGKCKKMAAELVKSFLKEHQEKMEAVDLSNYTIIG
EEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MNVTPWEVEGVIDYSKLIEEFGMQPFSEVLPEIDNPHILMRRGAIFGHRDYWRIIEAMQK
CCCCCEEEHHHHHHHHHHHHHCCCCHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHC
KEPWAVMSGFMPSGLPHFGHKMTMDEIVWHQSAGGKAFVAIADMEAHSVRGLSWEKTREL
CCCHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEEECCCHHHCCCCCHHHHHHH
GMLYIKSIIALGLREDAVIYFQSKSSHVKDLAFELSAEVNFSELRAIYGFNSDTSLAKMF
HHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHH
VTAIQAADILHPQLSDFGGPKPVVVPVGADQDPHMRLTRDLAARISIFSFEPVEGGVRVR
HHHHHHHHHCCCHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHEEEEECCCCCCCEEE
SRKGAEYLSSLRDLEFDKKIYEEHMDIFGEAEEIERAVRKIEVEIGGFAFIPPSSTYHRF
CCCCHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEECCEEECCCCCCCHHH
TTGLTGGKMSSSKPESYISLLDPPEEGAKKVMKAFTGGRATAEEQRRLGGEPDRCVVFEL
HCCCCCCCCCCCCCHHHHEECCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEEEE
YSFHLIDSDEELNQIEAECREGRLLCGKCKKMAAELVKSFLKEHQEKMEAVDLSNYTIIG
EEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475