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The map label for this gene is fad-4

Identifier: 11499231

GI number: 11499231

Start: 1471664

End: 1472428

Strand: Reverse

Name: fad-4

Synonym: AF1641

Alternate gene names: 11499231

Gene position: 1472428-1471664 (Counterclockwise)

Preceding gene: 11499232

Following gene: 11499226

Centisome position: 67.59

GC content: 49.67

Gene sequence:

>765_bases
ATGAAAAATCTGAAGTACCAGGAAAGGGGGAAAGTCGGGATAATTTCACTAAACCGGCCCGAAACCAGAAACGCTCTGTC
TCTCGAACTTCTTCAAGAGCTTGAAGACCTGATAAGGAAAATATCCGAGGAAAGGCTTGTGAGAGTCGTAGTAATTAGGG
GAGAGGGAAGGGCTTTCTCCTCCGGGCACGACCTCAAAGAGATTCTCGACAGGCATCCGATAGAGGTTGAGAAACTCTTC
AACCAGTGCTACAGGGCGATGCTCGCCATTAGAGATGCACCTCAGCCCTACATCGCCATGGTTCAGGGTGTTGCCACTGC
CGCAGGCTGTCAACTCGTAGCAGCTTGCGATATGGCTGTAGCGGCAAAATCCGCCCTCTTTGCAACTCCCGGTGTAAAAA
TAGGCCTCTTCTGCTACACGCCAATAGCCTTCGTAAGCAGAGCGGTTGGACGGAAGAAAGCCTTTGAGATGGGCTTCACG
GGGGAATTCATTACTGCTGATGAAGCTTTGCAGTTCGGGCTCGTAAACAGGGTTGTTGAGGATGAAAAGCTTGAGGAGGA
AACCATGAAGCTTGCCGAAAGTGTTGCGAGATACAGCCTAAATGTCCTTGAATCGGGAAAGAGGTTCTTCTACAAGCAGC
TATTTATGGAGGACTTTCAGGCATTGGCCTTTGCAACAGAGTCCATTTCACTGTACAGCTCCACGGAAGATGCTAAAGAG
GGGATAAGGGCCTTCTTTGAGAAAAGGGAGCCGATGTGGGAATGA

Upstream 100 bases:

>100_bases
ATAAAGAACCTCGAAACGCAGGAGCAGGTTGTTGTGGCTGAGGAGGAGCTTTTCAGCATCCTTCAGTAAGTTTTTTAATT
AATCATAACCTTATCCATTC

Downstream 100 bases:

>100_bases
AAAGGAGTGGTAACGATAAAGCCAAAAAATAAAACTAAAATTCAACAAACATTTCCCTCTTTGTGCCGCAAACTGGGCAG
AATTCAGGCGCCTCATCAAC

Product: enoyl-CoA hydratase (fad-4)

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKNLKYQERGKVGIISLNRPETRNALSLELLQELEDLIRKISEERLVRVVVIRGEGRAFSSGHDLKEILDRHPIEVEKLF
NQCYRAMLAIRDAPQPYIAMVQGVATAAGCQLVAACDMAVAAKSALFATPGVKIGLFCYTPIAFVSRAVGRKKAFEMGFT
GEFITADEALQFGLVNRVVEDEKLEEETMKLAESVARYSLNVLESGKRFFYKQLFMEDFQALAFATESISLYSSTEDAKE
GIRAFFEKREPMWE

Sequences:

>Translated_254_residues
MKNLKYQERGKVGIISLNRPETRNALSLELLQELEDLIRKISEERLVRVVVIRGEGRAFSSGHDLKEILDRHPIEVEKLF
NQCYRAMLAIRDAPQPYIAMVQGVATAAGCQLVAACDMAVAAKSALFATPGVKIGLFCYTPIAFVSRAVGRKKAFEMGFT
GEFITADEALQFGLVNRVVEDEKLEEETMKLAESVARYSLNVLESGKRFFYKQLFMEDFQALAFATESISLYSSTEDAKE
GIRAFFEKREPMWE
>Mature_254_residues
MKNLKYQERGKVGIISLNRPETRNALSLELLQELEDLIRKISEERLVRVVVIRGEGRAFSSGHDLKEILDRHPIEVEKLF
NQCYRAMLAIRDAPQPYIAMVQGVATAAGCQLVAACDMAVAAKSALFATPGVKIGLFCYTPIAFVSRAVGRKKAFEMGFT
GEFITADEALQFGLVNRVVEDEKLEEETMKLAESVARYSLNVLESGKRFFYKQLFMEDFQALAFATESISLYSSTEDAKE
GIRAFFEKREPMWE

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=241, Percent_Identity=46.0580912863071, Blast_Score=218, Evalue=4e-57,
Organism=Homo sapiens, GI194097323, Length=263, Percent_Identity=33.4600760456274, Blast_Score=121, Evalue=6e-28,
Organism=Homo sapiens, GI70995211, Length=183, Percent_Identity=31.6939890710383, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI37594471, Length=211, Percent_Identity=26.5402843601896, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI37594469, Length=211, Percent_Identity=26.5402843601896, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI45643119, Length=211, Percent_Identity=29.3838862559242, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI260275230, Length=211, Percent_Identity=29.3838862559242, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI260274832, Length=211, Percent_Identity=29.3838862559242, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI4502327, Length=268, Percent_Identity=27.9850746268657, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI20127408, Length=205, Percent_Identity=26.3414634146341, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI68989263, Length=194, Percent_Identity=26.8041237113402, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=256, Percent_Identity=33.203125, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1788597, Length=265, Percent_Identity=31.3207547169811, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI87082183, Length=254, Percent_Identity=29.9212598425197, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787660, Length=268, Percent_Identity=29.1044776119403, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI221142681, Length=259, Percent_Identity=28.1853281853282, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790281, Length=202, Percent_Identity=28.2178217821782, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788682, Length=189, Percent_Identity=28.042328042328, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17540306, Length=236, Percent_Identity=40.6779661016949, Blast_Score=166, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17554946, Length=255, Percent_Identity=32.9411764705882, Blast_Score=130, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI25145438, Length=245, Percent_Identity=30.6122448979592, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17560910, Length=256, Percent_Identity=27.734375, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=210, Percent_Identity=30, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17534483, Length=195, Percent_Identity=29.2307692307692, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17540714, Length=180, Percent_Identity=30, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=230, Percent_Identity=26.0869565217391, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=204, Percent_Identity=27.9411764705882, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17558304, Length=214, Percent_Identity=23.3644859813084, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI19922422, Length=248, Percent_Identity=37.9032258064516, Blast_Score=177, Evalue=6e-45,
Organism=Drosophila melanogaster, GI20129971, Length=251, Percent_Identity=32.2709163346614, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24653477, Length=251, Percent_Identity=32.2709163346614, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24653139, Length=262, Percent_Identity=29.3893129770992, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24654903, Length=221, Percent_Identity=28.5067873303167, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28571729, Length=181, Percent_Identity=27.6243093922652, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28571730, Length=181, Percent_Identity=27.6243093922652, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21357171, Length=262, Percent_Identity=24.4274809160305, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI19920382, Length=251, Percent_Identity=23.5059760956175, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24650670, Length=244, Percent_Identity=23.7704918032787, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28641; Mature: 28641

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLKYQERGKVGIISLNRPETRNALSLELLQELEDLIRKISEERLVRVVVIRGEGRAFS
CCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCC
SGHDLKEILDRHPIEVEKLFNQCYRAMLAIRDAPQPYIAMVQGVATAAGCQLVAACDMAV
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AAKSALFATPGVKIGLFCYTPIAFVSRAVGRKKAFEMGFTGEFITADEALQFGLVNRVVE
HHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHH
DEKLEEETMKLAESVARYSLNVLESGKRFFYKQLFMEDFQALAFATESISLYSSTEDAKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
GIRAFFEKREPMWE
HHHHHHHHCCCCCC
>Mature Secondary Structure
MKNLKYQERGKVGIISLNRPETRNALSLELLQELEDLIRKISEERLVRVVVIRGEGRAFS
CCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCC
SGHDLKEILDRHPIEVEKLFNQCYRAMLAIRDAPQPYIAMVQGVATAAGCQLVAACDMAV
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AAKSALFATPGVKIGLFCYTPIAFVSRAVGRKKAFEMGFTGEFITADEALQFGLVNRVVE
HHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHH
DEKLEEETMKLAESVARYSLNVLESGKRFFYKQLFMEDFQALAFATESISLYSSTEDAKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
GIRAFFEKREPMWE
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA