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The map label for this gene is hisS

Identifier: 11499232

GI number: 11499232

Start: 1472460

End: 1473689

Strand: Reverse

Name: hisS

Synonym: AF1642

Alternate gene names: 11499232

Gene position: 1473689-1472460 (Counterclockwise)

Preceding gene: 11499242

Following gene: 11499231

Centisome position: 67.65

GC content: 51.71

Gene sequence:

>1230_bases
GTGAAAATCGAACGACCCAGAGGCACGAGAGATTTTCTTCCTGACGAGATGGAGAGAAGGAGAGAAATAGAGAAGCGAAT
GAGAAAAATCGCCGAGAGCTTCGGATACAGGGAGGTTGCAACGCCAACTTTCGAGTACCTCGAGCTTTTCACGAGGAAGT
CCGGGGAGGGGATTATTGAGGAGATGTACGTTTTCAAGGACAAATCTGGCAGAGACCTCGCTCTAAGGCCTGAGCTTACC
GCTCCGGTAATGAGGATGTTTGTAAACGAGTGCAGCGTGATGCCAAAGCCCCTCCGCTTTTACTACTTCGCAAACTGCTT
CCGCTACGAGAGGCCGCAGAAGGGAAGGTATCGAGAATTCTGGCAGTTCGGCGTTGAGCTGATTGGTTCTGAATCGTACC
TCGCAGATGCTGAAGTCATAATCCTTGCAGATAAAATCCTGAAAGATGTGGGAGTGAATTTCAGCCTTGAAATAGGCCAC
GTCGGCATCATGAGGCACCTCCTGAAGCCCATCGGTGAGGATAGGGCTTCAAAGGTGATGAGGCTGATTGATAAGGGGGA
CAGGGAGGGGCTTGAAAGCTACCTTGCCGAAATCAGGGTCAATGAAGATTTGAGGGACAAAATCTTCAGCCTGCTTGAGC
TAAAGGGAGACGAGTCGGTTATTGAAGAGGCCAAGGAGATTATTGACTACGACTTCGGGCATCTTGAGAGTCTTTCCGCA
CTTCTCAGAGATGTTGGCGTGGACTTCACGCTCAACCTCGGAATAGCGAGGGGGCTCGACTACTACACGGGCGTCGTATT
TGAGTGCTACGCTGAAGGGCTCGGAGCGCAGAAGCAGGTTTGCGGTGGAGGGAGCTACGAGCTTTCCTCACTTTTCGGCG
GGCCTGTAACTCCTTCGACGGGATTCGCTATAGGCTTTGACAGGGTTTGTGAGGCTTGCAGCGTTGAAGCGGGAGAAAAG
AGTGTTGTTGCCGTCGTCTCCTTTAAGGGGCTGGAAAGCCAGGCCTTCAGGGTTGCAAGCATGCTGAGGGAAAGGGGCTT
CACTGCTGTTGTCGATGTGATGGGGAGAAATCTGAAAAAGCAGATGAGCTTTGCCAGCGAAATGGGGGTAAAGTATGCGG
TCATACTCGGCCCGGATGAGGTTAAGAGTGGGAGAGTTGCGATAAAGAACCTCGAAACGCAGGAGCAGGTTGTTGTGGCT
GAGGAGGAGCTTTTCAGCATCCTTCAGTAA

Upstream 100 bases:

>100_bases
CTGAATGTGCGCTTCAAACAAATAAAGTTTTTTTGAGTTTTTATGCCCACAAATTCGCTCAACTCTAAAACCTTTTAAAC
ACCCTTGCCGAAGCTTGAGC

Downstream 100 bases:

>100_bases
GTTTTTTAATTAATCATAACCTTATCCATTCATGAAAAATCTGAAGTACCAGGAAAGGGGGAAAGTCGGGATAATTTCAC
TAAACCGGCCCGAAACCAGA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MKIERPRGTRDFLPDEMERRREIEKRMRKIAESFGYREVATPTFEYLELFTRKSGEGIIEEMYVFKDKSGRDLALRPELT
APVMRMFVNECSVMPKPLRFYYFANCFRYERPQKGRYREFWQFGVELIGSESYLADAEVIILADKILKDVGVNFSLEIGH
VGIMRHLLKPIGEDRASKVMRLIDKGDREGLESYLAEIRVNEDLRDKIFSLLELKGDESVIEEAKEIIDYDFGHLESLSA
LLRDVGVDFTLNLGIARGLDYYTGVVFECYAEGLGAQKQVCGGGSYELSSLFGGPVTPSTGFAIGFDRVCEACSVEAGEK
SVVAVVSFKGLESQAFRVASMLRERGFTAVVDVMGRNLKKQMSFASEMGVKYAVILGPDEVKSGRVAIKNLETQEQVVVA
EEELFSILQ

Sequences:

>Translated_409_residues
MKIERPRGTRDFLPDEMERRREIEKRMRKIAESFGYREVATPTFEYLELFTRKSGEGIIEEMYVFKDKSGRDLALRPELT
APVMRMFVNECSVMPKPLRFYYFANCFRYERPQKGRYREFWQFGVELIGSESYLADAEVIILADKILKDVGVNFSLEIGH
VGIMRHLLKPIGEDRASKVMRLIDKGDREGLESYLAEIRVNEDLRDKIFSLLELKGDESVIEEAKEIIDYDFGHLESLSA
LLRDVGVDFTLNLGIARGLDYYTGVVFECYAEGLGAQKQVCGGGSYELSSLFGGPVTPSTGFAIGFDRVCEACSVEAGEK
SVVAVVSFKGLESQAFRVASMLRERGFTAVVDVMGRNLKKQMSFASEMGVKYAVILGPDEVKSGRVAIKNLETQEQVVVA
EEELFSILQ
>Mature_409_residues
MKIERPRGTRDFLPDEMERRREIEKRMRKIAESFGYREVATPTFEYLELFTRKSGEGIIEEMYVFKDKSGRDLALRPELT
APVMRMFVNECSVMPKPLRFYYFANCFRYERPQKGRYREFWQFGVELIGSESYLADAEVIILADKILKDVGVNFSLEIGH
VGIMRHLLKPIGEDRASKVMRLIDKGDREGLESYLAEIRVNEDLRDKIFSLLELKGDESVIEEAKEIIDYDFGHLESLSA
LLRDVGVDFTLNLGIARGLDYYTGVVFECYAEGLGAQKQVCGGGSYELSSLFGGPVTPSTGFAIGFDRVCEACSVEAGEK
SVVAVVSFKGLESQAFRVASMLRERGFTAVVDVMGRNLKKQMSFASEMGVKYAVILGPDEVKSGRVAIKNLETQEQVVVA
EEELFSILQ

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI15029520, Length=454, Percent_Identity=26.6519823788546, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI6996014, Length=446, Percent_Identity=26.2331838565022, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1788861, Length=434, Percent_Identity=32.9493087557604, Blast_Score=209, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI71993693, Length=455, Percent_Identity=24.3956043956044, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71993686, Length=452, Percent_Identity=24.5575221238938, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6325290, Length=458, Percent_Identity=24.4541484716157, Blast_Score=102, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24643061, Length=462, Percent_Identity=27.7056277056277, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24643059, Length=481, Percent_Identity=26.6112266112266, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_ARCFU (O28631)

Other databases:

- EMBL:   AE000782
- PIR:   A69455
- RefSeq:   NP_070470.1
- ProteinModelPortal:   O28631
- SMR:   O28631
- GeneID:   1484865
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF1642
- NMPDR:   fig|224325.1.peg.1631
- TIGR:   AF_1642
- HOGENOM:   HBG616575
- OMA:   YYFANCF
- ProtClustDB:   PRK00037
- BioCyc:   AFUL224325:AF_1642-MONOMER
- BRENDA:   6.1.1.21
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 46218; Mature: 46218

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIERPRGTRDFLPDEMERRREIEKRMRKIAESFGYREVATPTFEYLELFTRKSGEGIIE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHCCCCHHHH
EMYVFKDKSGRDLALRPELTAPVMRMFVNECSVMPKPLRFYYFANCFRYERPQKGRYREF
HHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHH
WQFGVELIGSESYLADAEVIILADKILKDVGVNFSLEIGHVGIMRHLLKPIGEDRASKVM
HHHHHHHHCCCCHHCCCHHEEHHHHHHHHHCCCEEEECCHHHHHHHHHHHHCCHHHHHHH
RLIDKGDREGLESYLAEIRVNEDLRDKIFSLLELKGDESVIEEAKEIIDYDFGHLESLSA
HHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHH
LLRDVGVDFTLNLGIARGLDYYTGVVFECYAEGLGAQKQVCGGGSYELSSLFGGPVTPST
HHHHHCCCEEEECHHHHCCHHHHHHHHHHHHHCCCCCHHHCCCCCEEHHHHCCCCCCCCC
GFAIGFDRVCEACSVEAGEKSVVAVVSFKGLESQAFRVASMLRERGFTAVVDVMGRNLKK
CCEECHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
QMSFASEMGVKYAVILGPDEVKSGRVAIKNLETQEQVVVAEEELFSILQ
HHHHHHHCCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKIERPRGTRDFLPDEMERRREIEKRMRKIAESFGYREVATPTFEYLELFTRKSGEGIIE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHCCCCHHHH
EMYVFKDKSGRDLALRPELTAPVMRMFVNECSVMPKPLRFYYFANCFRYERPQKGRYREF
HHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHH
WQFGVELIGSESYLADAEVIILADKILKDVGVNFSLEIGHVGIMRHLLKPIGEDRASKVM
HHHHHHHHCCCCHHCCCHHEEHHHHHHHHHCCCEEEECCHHHHHHHHHHHHCCHHHHHHH
RLIDKGDREGLESYLAEIRVNEDLRDKIFSLLELKGDESVIEEAKEIIDYDFGHLESLSA
HHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHH
LLRDVGVDFTLNLGIARGLDYYTGVVFECYAEGLGAQKQVCGGGSYELSSLFGGPVTPST
HHHHHCCCEEEECHHHHCCHHHHHHHHHHHHHCCCCCHHHCCCCCEEHHHHCCCCCCCCC
GFAIGFDRVCEACSVEAGEKSVVAVVSFKGLESQAFRVASMLRERGFTAVVDVMGRNLKK
CCEECHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
QMSFASEMGVKYAVILGPDEVKSGRVAIKNLETQEQVVVAEEELFSILQ
HHHHHHHCCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475